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The map label for this gene is holB [H]

Identifier: 120598612

GI number: 120598612

Start: 2066368

End: 2067324

Strand: Direct

Name: holB [H]

Synonym: Sputw3181_1796

Alternate gene names: 120598612

Gene position: 2066368-2067324 (Clockwise)

Preceding gene: 120598611

Following gene: 120598613

Centisome position: 43.89

GC content: 45.77

Gene sequence:

>957_bases
ATGGTAGCCGAGTCCGGATTTACGGCTGAGCAATTGCCTTGGCTCGAAGGGCCAAGGCAAGCATTTTTAGCACAATTACA
ATGCCAGAAAATCCCCCATGCCCAACTCGTTGGTATCGATGCTGCGTTCGGTGGTGAGCTGTTTAGTGTGTTTATGGCGC
GTGCTGCCCTGTGTTTAGCGCCTAATTCGGCAGGCGCATGTGGTTTTTGTAAGTCGTGCCAGTTATTCGCTGCGGGCAAT
CATCCCGATTTTTACCAAATAGAAGCCGATGGTAATCAAATTAAGGTCGATCAAATTCGTGAACTTTGCGTGCGCCTTAC
GGCAACCGCGCAGCAAAGTGGTCGCCGTGTAGCGATTATTCATCACAGTGAGCGCCTTAATTCTGCATCGGCTAATGCTC
TGCTTAAAACCCTTGAAGAGCCGGGTAAGGACACGCTGTTAATATTACAGTCGGATACACCGGCACGTTTAATGGCTACC
ATCAGTAGTCGCTGTCAGCGCATTCCTTTTGTGGCGCCGAATAAGGCGATGATCAAAACCTGGTTAGCTCAGCATTGTCA
TATTCAAGAGGATGTGACTTGGTGCCTAAGTGTGGTGGGTGGACCATTAAAATTGGCCGAGAGTTTGCTAACAGATGGTA
CACAATCGAGCCGCTATCAATCCCTGCTTCAGTTTCGCAAAGATTGGGCGCAAAGTTTGTCGTCTGGTCATCTGTGTGCT
AGTTTATTAACAGTGAACGAACAGCAAATAATTGATGCACTTAAGGTTTTATATCTACTACTGCGACAAATTTTATTGAA
GAACAGTCACCAAGATGCGTTTATTCAGGCGCAAGTAGGGAACTTGGCGGCAAAGATCATGGGAATGTGTCATAGCTTAA
GCACAATGCCGACTGTCAATTATTTGGGCTTATGTCAAAGTTATGTCTTAGAATATAGAGAAATAACAAAAAAGTAA

Upstream 100 bases:

>100_bases
TAAAGCTTGCCAGCGAAGATGACAGTATTGTGGTTATCGATGCGAGTCAAACCATGGCTGAAGTTCATAAAGATATTTTA
GCCGTGCTACAGGCGATGGC

Downstream 100 bases:

>100_bases
CATGCCATGGTCGATCTTATTGTTAATTTTGACACCTTACATCAGCTCTATCGGGCTTATATGCCTTTTATCAAGCCTGC
GGGATTGTTTGTTACCACCA

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVAESGFTAEQLPWLEGPRQAFLAQLQCQKIPHAQLVGIDAAFGGELFSVFMARAALCLAPNSAGACGFCKSCQLFAAGN
HPDFYQIEADGNQIKVDQIRELCVRLTATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLILQSDTPARLMAT
ISSRCQRIPFVAPNKAMIKTWLAQHCHIQEDVTWCLSVVGGPLKLAESLLTDGTQSSRYQSLLQFRKDWAQSLSSGHLCA
SLLTVNEQQIIDALKVLYLLLRQILLKNSHQDAFIQAQVGNLAAKIMGMCHSLSTMPTVNYLGLCQSYVLEYREITKK

Sequences:

>Translated_318_residues
MVAESGFTAEQLPWLEGPRQAFLAQLQCQKIPHAQLVGIDAAFGGELFSVFMARAALCLAPNSAGACGFCKSCQLFAAGN
HPDFYQIEADGNQIKVDQIRELCVRLTATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLILQSDTPARLMAT
ISSRCQRIPFVAPNKAMIKTWLAQHCHIQEDVTWCLSVVGGPLKLAESLLTDGTQSSRYQSLLQFRKDWAQSLSSGHLCA
SLLTVNEQQIIDALKVLYLLLRQILLKNSHQDAFIQAQVGNLAAKIMGMCHSLSTMPTVNYLGLCQSYVLEYREITKK
>Mature_318_residues
MVAESGFTAEQLPWLEGPRQAFLAQLQCQKIPHAQLVGIDAAFGGELFSVFMARAALCLAPNSAGACGFCKSCQLFAAGN
HPDFYQIEADGNQIKVDQIRELCVRLTATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLILQSDTPARLMAT
ISSRCQRIPFVAPNKAMIKTWLAQHCHIQEDVTWCLSVVGGPLKLAESLLTDGTQSSRYQSLLQFRKDWAQSLSSGHLCA
SLLTVNEQQIIDALKVLYLLLRQILLKNSHQDAFIQAQVGNLAAKIMGMCHSLSTMPTVNYLGLCQSYVLEYREITKK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=276, Percent_Identity=30.0724637681159, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786676, Length=140, Percent_Identity=30.7142857142857, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34986; Mature: 34986

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAESGFTAEQLPWLEGPRQAFLAQLQCQKIPHAQLVGIDAAFGGELFSVFMARAALCLA
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHCC
PNSAGACGFCKSCQLFAAGNHPDFYQIEADGNQIKVDQIRELCVRLTATAQQSGRRVAII
CCCCCCCCHHHCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLILQSDTPARLMATISSRCQRIPFVAPNKAMIKT
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WLAQHCHIQEDVTWCLSVVGGPLKLAESLLTDGTQSSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTVNEQQIIDALKVLYLLLRQILLKNSHQDAFIQAQVGNLAAKIMGMCHSLSTMPTVN
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLGLCQSYVLEYREITKK
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVAESGFTAEQLPWLEGPRQAFLAQLQCQKIPHAQLVGIDAAFGGELFSVFMARAALCLA
CCCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHCC
PNSAGACGFCKSCQLFAAGNHPDFYQIEADGNQIKVDQIRELCVRLTATAQQSGRRVAII
CCCCCCCCHHHCCEEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLILQSDTPARLMATISSRCQRIPFVAPNKAMIKT
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
WLAQHCHIQEDVTWCLSVVGGPLKLAESLLTDGTQSSRYQSLLQFRKDWAQSLSSGHLCA
HHHHHCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
SLLTVNEQQIIDALKVLYLLLRQILLKNSHQDAFIQAQVGNLAAKIMGMCHSLSTMPTVN
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCHH
YLGLCQSYVLEYREITKK
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]