Click here to switch to the map view.

The map label for this gene is tmk

Identifier: 120598611

GI number: 120598611

Start: 2065748

End: 2066374

Strand: Direct

Name: tmk

Synonym: Sputw3181_1795

Alternate gene names: 120598611

Gene position: 2065748-2066374 (Clockwise)

Preceding gene: 120598610

Following gene: 120598612

Centisome position: 43.87

GC content: 47.53

Gene sequence:

>627_bases
ATGTCAGTTCTTGCAAAATTTATCGTTATTGAAGGTTTAGAAGGCGCTGGTAAGTCGAGTGCAATTACCCTTATTCGGGA
TTTTATTGAGAAGCACACTGGGCGAGCACCTATTTGTACCCGAGAGCCCGGAGGTACACCGCTCGCTGAACGGATCCGTG
ATTTAGTCAAAATTGCCGATCCGAGCGATCCCCTCTGCGACGAGTCTGAATGTTTGCTTATATATGCCGCTAGAGCGCAG
TTGGTTGCTAATGTAATTAAACCTGCACTTGCGCAAGGGCAATGGGTACTTGGAGACAGACACAATCTATCATCACTGGC
ATATCAAGGTGGTGGACGAGGGTTAATGCCCTTAGTGGAGGCGGTGAGTGCTGCAACACTAAAGGGATTTAAACCCGATC
TCACCTTATATTTAGATTTAGATCCTAAGCTTGGACTCACCCGCGCGGCTAAACGTGGTGAACTCGATAGAATCGAGCAG
CAGACCATTGATTTCTTTGAGCGCGCCCGCGCAACTTATCTAAAGCTTGCCAGCGAAGATGACAGTATTGTGGTTATCGA
TGCGAGTCAAACCATGGCTGAAGTTCATAAAGATATTTTAGCCGTGCTACAGGCGATGGCATGGTAG

Upstream 100 bases:

>100_bases
CTAGAAGAACACAACCATGCTGTAGACATATATCAGCGTAAGATAAAACCGGCGCCCCCAGAAAAATCACCAAAGTAAAA
ATGCAGAGAAAGAAAATCTA

Downstream 100 bases:

>100_bases
CCGAGTCCGGATTTACGGCTGAGCAATTGCCTTGGCTCGAAGGGCCAAGGCAAGCATTTTTAGCACAATTACAATGCCAG
AAAATCCCCCATGCCCAACT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSVLAKFIVIEGLEGAGKSSAITLIRDFIEKHTGRAPICTREPGGTPLAERIRDLVKIADPSDPLCDESECLLIYAARAQ
LVANVIKPALAQGQWVLGDRHNLSSLAYQGGGRGLMPLVEAVSAATLKGFKPDLTLYLDLDPKLGLTRAAKRGELDRIEQ
QTIDFFERARATYLKLASEDDSIVVIDASQTMAEVHKDILAVLQAMAW

Sequences:

>Translated_208_residues
MSVLAKFIVIEGLEGAGKSSAITLIRDFIEKHTGRAPICTREPGGTPLAERIRDLVKIADPSDPLCDESECLLIYAARAQ
LVANVIKPALAQGQWVLGDRHNLSSLAYQGGGRGLMPLVEAVSAATLKGFKPDLTLYLDLDPKLGLTRAAKRGELDRIEQ
QTIDFFERARATYLKLASEDDSIVVIDASQTMAEVHKDILAVLQAMAW
>Mature_207_residues
SVLAKFIVIEGLEGAGKSSAITLIRDFIEKHTGRAPICTREPGGTPLAERIRDLVKIADPSDPLCDESECLLIYAARAQL
VANVIKPALAQGQWVLGDRHNLSSLAYQGGGRGLMPLVEAVSAATLKGFKPDLTLYLDLDPKLGLTRAAKRGELDRIEQQ
TIDFFERARATYLKLASEDDSIVVIDASQTMAEVHKDILAVLQAMAW

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=194, Percent_Identity=55.1546391752577, Blast_Score=207, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 22612; Mature: 22481

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLAKFIVIEGLEGAGKSSAITLIRDFIEKHTGRAPICTREPGGTPLAERIRDLVKIAD
CHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PSDPLCDESECLLIYAARAQLVANVIKPALAQGQWVLGDRHNLSSLAYQGGGRGLMPLVE
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCHHHHHH
AVSAATLKGFKPDLTLYLDLDPKLGLTRAAKRGELDRIEQQTIDFFERARATYLKLASED
HHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCC
DSIVVIDASQTMAEVHKDILAVLQAMAW
CCEEEEECHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVLAKFIVIEGLEGAGKSSAITLIRDFIEKHTGRAPICTREPGGTPLAERIRDLVKIAD
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
PSDPLCDESECLLIYAARAQLVANVIKPALAQGQWVLGDRHNLSSLAYQGGGRGLMPLVE
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHCCCCCCHHHHHH
AVSAATLKGFKPDLTLYLDLDPKLGLTRAAKRGELDRIEQQTIDFFERARATYLKLASED
HHHHHHHCCCCCCEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCC
DSIVVIDASQTMAEVHKDILAVLQAMAW
CCEEEEECHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]