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The map label for this gene is sdhB

Identifier: 120598555

GI number: 120598555

Start: 2001063

End: 2001770

Strand: Direct

Name: sdhB

Synonym: Sputw3181_1738

Alternate gene names: 120598555

Gene position: 2001063-2001770 (Clockwise)

Preceding gene: 120598554

Following gene: 120598556

Centisome position: 42.5

GC content: 45.06

Gene sequence:

>708_bases
ATGAAATTGGAATTTGCAGTTTATCGTTACAATCCTGATGTAGATACTAAACCATACATGCAGGATTACAGCATAGAAGT
GGCAGATGGCTCAGACATGATGGTGCTTGATGCACTGATCAAGTTAAAAGAGCAAGATCCGACGCTATCATTCCGCCGCT
CATGCCGTGAAGGTGTGTGTGGTAGCGATGGCGTGAACATGAATGGTAAAAATGGTCTGGCGTGTATTACCCCGATTTCA
ACCTTTAAAGGTAAGAAATTGGAGATCCGTCCGTTACCAGGTATGCCTGTTGTGCGTGACTTAGTGGTTGATCTTACTCA
GTTCTATAAGCAGTATGAGAAGATCAAGCCTTATCTCATCAATGATGAGAAAATGCCTGCCCGTGAACATTTACAATCCC
CTGAAGAGCGTGCTCATTTAGACGGGTTGTACGAATGTATCATGTGTGCATGCTGTTCTACGGCTTGTCCATCATTCTGG
TGGAACCCTGATAAGTTTATCGGTCCTAGCGGTTTGCTCCATGCATACCGTTTCTTGATCGACAGTCGCGATACAGCTAC
AGAAGAGCGTTTATCTGAGCTGGACGACGCCTATAGCGTGTTCCGTTGCCACGGCATCATGAACTGCGTTGATGTGTGTC
CAAAAGGTCTTAATCCGACTAAAGCAATTGGTCATATTAAGTCCATGCTGTTGAAGCGAGCAGTGTAA

Upstream 100 bases:

>100_bases
ACCCTGTGACAGAGACCATGGACCGTCGTGCTGTGAATATGGCACCTAAGTTACGTGAAGCATTCCCACCGAAGAAGCGT
ACCTACTAGGAGTTGCAAAG

Downstream 100 bases:

>100_bases
TGCGAGAACCCGAGCTAAAACCAATAATAATATAGCTCTTGAAAGAGTCACTTTCTGAGTTTAGAAGGTGGCTCTTTTGC
GTATGTAATGGAAGACAACG

Product: succinate dehydrogenase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKLEFAVYRYNPDVDTKPYMQDYSIEVADGSDMMVLDALIKLKEQDPTLSFRRSCREGVCGSDGVNMNGKNGLACITPIS
TFKGKKLEIRPLPGMPVVRDLVVDLTQFYKQYEKIKPYLINDEKMPAREHLQSPEERAHLDGLYECIMCACCSTACPSFW
WNPDKFIGPSGLLHAYRFLIDSRDTATEERLSELDDAYSVFRCHGIMNCVDVCPKGLNPTKAIGHIKSMLLKRAV

Sequences:

>Translated_235_residues
MKLEFAVYRYNPDVDTKPYMQDYSIEVADGSDMMVLDALIKLKEQDPTLSFRRSCREGVCGSDGVNMNGKNGLACITPIS
TFKGKKLEIRPLPGMPVVRDLVVDLTQFYKQYEKIKPYLINDEKMPAREHLQSPEERAHLDGLYECIMCACCSTACPSFW
WNPDKFIGPSGLLHAYRFLIDSRDTATEERLSELDDAYSVFRCHGIMNCVDVCPKGLNPTKAIGHIKSMLLKRAV
>Mature_235_residues
MKLEFAVYRYNPDVDTKPYMQDYSIEVADGSDMMVLDALIKLKEQDPTLSFRRSCREGVCGSDGVNMNGKNGLACITPIS
TFKGKKLEIRPLPGMPVVRDLVVDLTQFYKQYEKIKPYLINDEKMPAREHLQSPEERAHLDGLYECIMCACCSTACPSFW
WNPDKFIGPSGLLHAYRFLIDSRDTATEERLSELDDAYSVFRCHGIMNCVDVCPKGLNPTKAIGHIKSMLLKRAV

Specific function: Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth [H]

COG id: COG0479

COG function: function code C; Succinate dehydrogenase/fumarate reductase, Fe-S protein subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI115387094, Length=231, Percent_Identity=57.5757575757576, Blast_Score=270, Evalue=6e-73,
Organism=Escherichia coli, GI1786943, Length=236, Percent_Identity=77.1186440677966, Blast_Score=392, Evalue=1e-111,
Organism=Escherichia coli, GI1790596, Length=228, Percent_Identity=38.1578947368421, Blast_Score=153, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17533915, Length=232, Percent_Identity=53.448275862069, Blast_Score=256, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6322987, Length=230, Percent_Identity=56.0869565217391, Blast_Score=263, Evalue=2e-71,
Organism=Drosophila melanogaster, GI17137106, Length=233, Percent_Identity=55.793991416309, Blast_Score=274, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24643156, Length=231, Percent_Identity=51.5151515151515, Blast_Score=251, Evalue=2e-67,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR004489 [H]

Pfam domain/function: NA

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 26631; Mature: 26631

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEFAVYRYNPDVDTKPYMQDYSIEVADGSDMMVLDALIKLKEQDPTLSFRRSCREGVC
CCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCC
GSDGVNMNGKNGLACITPISTFKGKKLEIRPLPGMPVVRDLVVDLTQFYKQYEKIKPYLI
CCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
NDEKMPAREHLQSPEERAHLDGLYECIMCACCSTACPSFWWNPDKFIGPSGLLHAYRFLI
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DSRDTATEERLSELDDAYSVFRCHGIMNCVDVCPKGLNPTKAIGHIKSMLLKRAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLEFAVYRYNPDVDTKPYMQDYSIEVADGSDMMVLDALIKLKEQDPTLSFRRSCREGVC
CCCEEEEEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCC
GSDGVNMNGKNGLACITPISTFKGKKLEIRPLPGMPVVRDLVVDLTQFYKQYEKIKPYLI
CCCCCCCCCCCCEEEECCHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEE
NDEKMPAREHLQSPEERAHLDGLYECIMCACCSTACPSFWWNPDKFIGPSGLLHAYRFLI
CCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
DSRDTATEERLSELDDAYSVFRCHGIMNCVDVCPKGLNPTKAIGHIKSMLLKRAV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 6388571; 8905232; 9278503; 6376123; 9298646 [H]