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The map label for this gene is sdhA

Identifier: 120598554

GI number: 120598554

Start: 1999285

End: 2001051

Strand: Direct

Name: sdhA

Synonym: Sputw3181_1737

Alternate gene names: 120598554

Gene position: 1999285-2001051 (Clockwise)

Preceding gene: 120598553

Following gene: 120598555

Centisome position: 42.46

GC content: 47.37

Gene sequence:

>1767_bases
GTGAGTATTCCAGTACGCGAATTTGATGCAGTAGTGATAGGAGCGGGCGGTGCGGGTATGCGCGCAGCTCTGCAGATTTC
TAAAGAAGGTAAAAGCTGTGCGCTTTTATCAAAAGTTTTCCCAACACGTTCTCATACTGTGTCTGCACAGGGAGGTATTA
CCGTTGCGTTAGGTAATGCCCATGAAGATCACTGGGAACAACATATGTACGATACCGTTAAAGGTTCCGATTTTATCGGC
GACCAAGATGCTATCGAATTTATGTGTCAAACTGGTCCAGAAGCCATTATTGAACTTGAGCATATGGGGTTGCCTTTCTC
TCGTTTTGAAAATGGTAAGATTTATCAACGTCCTTTCGGCGGTCAATCTAAGAACTTTGGTGGTGAGCAGGCGGCGCGTA
CGGCGGCAGCTGCCGACCGTACAGGTCATGCTTTACTGCATTGTTTGTATCAGCAAAACGTTAAACACAAAACGCAAGTC
TTCTCTGAGTGGTATGCCTTAGATTTAGTGAAGAACGCTGATGGTGCTATCGTAGGCTGTACCGCGATTGAAATCGAAAC
AGGCGATATCGTTTACTTCAAAGCCAAAGCCACCGTTTTAGCAACGGGTGGTGCAGGTCGTATTTATGCGTCTACAACAA
ACGCACATATTAACACCGGTGACGGTGTCGGTATGGCAATGCGTGCTGGCGTACAAATGCAAGATATGGAAATGTGGCAA
TTCCACCCAACGGGCATCGCCGGCGCGGGTGTACTTGTGACTGAAGGTTGTCGTGGAGAAGGTGGATATCTTCTAAATAA
AGACGGCGAGCGTTTTATGGAGCGTTATGCGCCCAACGCGAAGGATTTAGCATCACGCGACGTAGTAGCACGTTCTATGA
TGACTGAAATCCGTGAAGGCCGTGGTTTAGATGGTCCATTAGGTCCACACTGTTTACTGAAGCTTGATCACTTAGGTAAA
GAAACATTAGAAGCACGTTTACCAGGCGTGTGTGAATTGTCTCGTACCTTTGCCCATATCGATCCGGCCGATGGCCCAAT
CCCAGTATTACCAACGTGTCACTATATGATGGGCGGTCTGCCAACTAAGGTTAGCGGTCAAGTGATTCGTAAGCATGAAG
ACGGCACTGAACAGGATGTTATCGGTTTATTCGCGGTAGGTGAGATTGCCTGTGTATCTGTACACGGTGCTAACCGTTTA
GGTGGCAACTCACTGCTTGACTTAGTGGTATTTGGTCGTGCAGCGGGACAACATTTAGGTAAGGCGCTCGATGAGACAGC
CGATCCTAAAGATGCGACCGATGCGGATATTCAAGCGTCACTTGCACGTCTGAACCGTTGGGAAAACAACAAAGATGGTG
AAGATCCAGCGCAAATCCGTAAGGATTTACAACTTTGCATGCAATTAAACTTCTCTGTATTCCGCCGTGGTGATGCTATG
GCTGAAGGCTTAGAGCAGTTAAAAGCGATTCGTAAGCGGTTAGAAAATGCTAAGTTGTCAGATAACTCCCGTGAATTCAA
CACGCAACGTATTGAATGTTTAGAGTTAGATAACTTAATGGCGACGGCGATTGCGACTGCTTATGCTGCCAACTTCCGTA
CTGAGAGCCGCGGCGCTCATTCGCGTGAAGATTATTTAGAGCGTGACGATGAAAATTGGTTATGCCACAGCTTGTTTGAC
CCTGTGACAGAGACCATGGACCGTCGTGCTGTGAATATGGCACCTAAGTTACGTGAAGCATTCCCACCGAAGAAGCGTAC
CTACTAG

Upstream 100 bases:

>100_bases
CGTTAAGTGCACGTCGTTACGTGGTGTTTTACAGTTCACCTTTGTCGTTACCGTCTTTTGTTATCTGGCGGCTGGCATTG
TTATTGTGTGGGGTGTCTAA

Downstream 100 bases:

>100_bases
GAGTTGCAAAGATGAAATTGGAATTTGCAGTTTATCGTTACAATCCTGATGTAGATACTAAACCATACATGCAGGATTAC
AGCATAGAAGTGGCAGATGG

Product: succinate dehydrogenase flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MSIPVREFDAVVIGAGGAGMRAALQISKEGKSCALLSKVFPTRSHTVSAQGGITVALGNAHEDHWEQHMYDTVKGSDFIG
DQDAIEFMCQTGPEAIIELEHMGLPFSRFENGKIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHCLYQQNVKHKTQV
FSEWYALDLVKNADGAIVGCTAIEIETGDIVYFKAKATVLATGGAGRIYASTTNAHINTGDGVGMAMRAGVQMQDMEMWQ
FHPTGIAGAGVLVTEGCRGEGGYLLNKDGERFMERYAPNAKDLASRDVVARSMMTEIREGRGLDGPLGPHCLLKLDHLGK
ETLEARLPGVCELSRTFAHIDPADGPIPVLPTCHYMMGGLPTKVSGQVIRKHEDGTEQDVIGLFAVGEIACVSVHGANRL
GGNSLLDLVVFGRAAGQHLGKALDETADPKDATDADIQASLARLNRWENNKDGEDPAQIRKDLQLCMQLNFSVFRRGDAM
AEGLEQLKAIRKRLENAKLSDNSREFNTQRIECLELDNLMATAIATAYAANFRTESRGAHSREDYLERDDENWLCHSLFD
PVTETMDRRAVNMAPKLREAFPPKKRTY

Sequences:

>Translated_588_residues
MSIPVREFDAVVIGAGGAGMRAALQISKEGKSCALLSKVFPTRSHTVSAQGGITVALGNAHEDHWEQHMYDTVKGSDFIG
DQDAIEFMCQTGPEAIIELEHMGLPFSRFENGKIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHCLYQQNVKHKTQV
FSEWYALDLVKNADGAIVGCTAIEIETGDIVYFKAKATVLATGGAGRIYASTTNAHINTGDGVGMAMRAGVQMQDMEMWQ
FHPTGIAGAGVLVTEGCRGEGGYLLNKDGERFMERYAPNAKDLASRDVVARSMMTEIREGRGLDGPLGPHCLLKLDHLGK
ETLEARLPGVCELSRTFAHIDPADGPIPVLPTCHYMMGGLPTKVSGQVIRKHEDGTEQDVIGLFAVGEIACVSVHGANRL
GGNSLLDLVVFGRAAGQHLGKALDETADPKDATDADIQASLARLNRWENNKDGEDPAQIRKDLQLCMQLNFSVFRRGDAM
AEGLEQLKAIRKRLENAKLSDNSREFNTQRIECLELDNLMATAIATAYAANFRTESRGAHSREDYLERDDENWLCHSLFD
PVTETMDRRAVNMAPKLREAFPPKKRTY
>Mature_587_residues
SIPVREFDAVVIGAGGAGMRAALQISKEGKSCALLSKVFPTRSHTVSAQGGITVALGNAHEDHWEQHMYDTVKGSDFIGD
QDAIEFMCQTGPEAIIELEHMGLPFSRFENGKIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHCLYQQNVKHKTQVF
SEWYALDLVKNADGAIVGCTAIEIETGDIVYFKAKATVLATGGAGRIYASTTNAHINTGDGVGMAMRAGVQMQDMEMWQF
HPTGIAGAGVLVTEGCRGEGGYLLNKDGERFMERYAPNAKDLASRDVVARSMMTEIREGRGLDGPLGPHCLLKLDHLGKE
TLEARLPGVCELSRTFAHIDPADGPIPVLPTCHYMMGGLPTKVSGQVIRKHEDGTEQDVIGLFAVGEIACVSVHGANRLG
GNSLLDLVVFGRAAGQHLGKALDETADPKDATDADIQASLARLNRWENNKDGEDPAQIRKDLQLCMQLNFSVFRRGDAMA
EGLEQLKAIRKRLENAKLSDNSREFNTQRIECLELDNLMATAIATAYAANFRTESRGAHSREDYLERDDENWLCHSLFDP
VTETMDRRAVNMAPKLREAFPPKKRTY

Specific function: Two distinct, membrane-bound, FAD-containing enzymes are responsible for the catalysis of fumarate and succinate interconversion; the fumarate reductase is used in anaerobic growth, and the succinate dehydrogenase is used in aerobic growth [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=550, Percent_Identity=54.1818181818182, Blast_Score=534, Evalue=1e-151,
Organism=Escherichia coli, GI1786942, Length=588, Percent_Identity=80.1020408163265, Blast_Score=930, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=573, Percent_Identity=39.4415357766143, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1788928, Length=564, Percent_Identity=29.2553191489362, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=592, Percent_Identity=48.6486486486487, Blast_Score=535, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI17550100, Length=588, Percent_Identity=48.469387755102, Blast_Score=529, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71986328, Length=78, Percent_Identity=47.4358974358974, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322701, Length=593, Percent_Identity=52.2765598650928, Blast_Score=576, Evalue=1e-165,
Organism=Saccharomyces cerevisiae, GI6322416, Length=554, Percent_Identity=54.6931407942238, Blast_Score=574, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6320788, Length=490, Percent_Identity=26.9387755102041, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322511, Length=487, Percent_Identity=24.0246406570842, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI17137288, Length=545, Percent_Identity=53.394495412844, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24655642, Length=545, Percent_Identity=53.394495412844, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24655647, Length=545, Percent_Identity=53.394495412844, Blast_Score=540, Evalue=1e-154,
Organism=Drosophila melanogaster, GI24663005, Length=548, Percent_Identity=48.9051094890511, Blast_Score=503, Evalue=1e-142,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR011281
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 64223; Mature: 64091

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPVREFDAVVIGAGGAGMRAALQISKEGKSCALLSKVFPTRSHTVSAQGGITVALGNA
CCCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCEEEEECCC
HEDHWEQHMYDTVKGSDFIGDQDAIEFMCQTGPEAIIELEHMGLPFSRFENGKIYQRPFG
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHEEEHHHCCCCHHHCCCCEEEECCCC
GQSKNFGGEQAARTAAAADRTGHALLHCLYQQNVKHKTQVFSEWYALDLVKNADGAIVGC
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCEEEE
TAIEIETGDIVYFKAKATVLATGGAGRIYASTTNAHINTGDGVGMAMRAGVQMQDMEMWQ
EEEEEECCCEEEEEEEEEEEEECCCCEEEEECCCCEEECCCCCCHHHHCCCCEECCHHEE
FHPTGIAGAGVLVTEGCRGEGGYLLNKDGERFMERYAPNAKDLASRDVVARSMMTEIREG
ECCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RGLDGPLGPHCLLKLDHLGKETLEARLPGVCELSRTFAHIDPADGPIPVLPTCHYMMGGL
CCCCCCCCCHHEEEEHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC
PTKVSGQVIRKHEDGTEQDVIGLFAVGEIACVSVHGANRLGGNSLLDLVVFGRAAGQHLG
CCCCCCHHHCCCCCCCCHHHEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
KALDETADPKDATDADIQASLARLNRWENNKDGEDPAQIRKDLQLCMQLNFSVFRRGDAM
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHH
AEGLEQLKAIRKRLENAKLSDNSREFNTQRIECLELDNLMATAIATAYAANFRTESRGAH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
SREDYLERDDENWLCHSLFDPVTETMDRRAVNMAPKLREAFPPKKRTY
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIPVREFDAVVIGAGGAGMRAALQISKEGKSCALLSKVFPTRSHTVSAQGGITVALGNA
CCCCCCCCEEEEECCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEECCCCEEEEECCC
HEDHWEQHMYDTVKGSDFIGDQDAIEFMCQTGPEAIIELEHMGLPFSRFENGKIYQRPFG
CHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHEEEHHHCCCCHHHCCCCEEEECCCC
GQSKNFGGEQAARTAAAADRTGHALLHCLYQQNVKHKTQVFSEWYALDLVKNADGAIVGC
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCCEEEE
TAIEIETGDIVYFKAKATVLATGGAGRIYASTTNAHINTGDGVGMAMRAGVQMQDMEMWQ
EEEEEECCCEEEEEEEEEEEEECCCCEEEEECCCCEEECCCCCCHHHHCCCCEECCHHEE
FHPTGIAGAGVLVTEGCRGEGGYLLNKDGERFMERYAPNAKDLASRDVVARSMMTEIREG
ECCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RGLDGPLGPHCLLKLDHLGKETLEARLPGVCELSRTFAHIDPADGPIPVLPTCHYMMGGL
CCCCCCCCCHHEEEEHHCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCC
PTKVSGQVIRKHEDGTEQDVIGLFAVGEIACVSVHGANRLGGNSLLDLVVFGRAAGQHLG
CCCCCCHHHCCCCCCCCHHHEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
KALDETADPKDATDADIQASLARLNRWENNKDGEDPAQIRKDLQLCMQLNFSVFRRGDAM
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCHHH
AEGLEQLKAIRKRLENAKLSDNSREFNTQRIECLELDNLMATAIATAYAANFRTESRGAH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
SREDYLERDDENWLCHSLFDPVTETMDRRAVNMAPKLREAFPPKKRTY
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]