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The map label for this gene is topB [H]

Identifier: 120598390

GI number: 120598390

Start: 1792824

End: 1794950

Strand: Direct

Name: topB [H]

Synonym: Sputw3181_1573

Alternate gene names: 120598390

Gene position: 1792824-1794950 (Clockwise)

Preceding gene: 120598389

Following gene: 120598391

Centisome position: 38.08

GC content: 46.69

Gene sequence:

>2127_bases
ATGATCCTCTATATCGCAGAAAAACCTAGCCTAGGGCGGGCCATTGCCGATGTATTGCCTAAACCCCATAAAAAAGGCGA
TGGATTTATCGAAGCCGCTAATGGCGATTGCGTGTCTTGGTGTGTCGGTCACTTGCTCGAACAAGCAGAACCTGATGCCT
ATAATCCTGAATTTAAAGCATGGAAATTTGAGCACTTACCGATAGTGCCAGACAAGTGGCAACTGACACCTAAGGCAGCA
ACCCGCAGTCAACTCACTGTGCTTAAACGATTAGTTAAACAAGCGAGTATGCTGGTTAATGCGGGCGATCCCGACCGTGA
AGGGCAGTTACTCGTGGATGAAGTGATTGCCTATCTTGGTGTGACGGGGGACAAGCTACATCAAACCCAGCGTTTACTGA
TCAGTGACTTAAACCCACAAGCTGTAAAACGGGCTCTAACGCAGCTACGCAGTAATCGCGAATTTATCCCATTATCTACC
TCTGCTTTAGCCCGTAGCCGTGCCGATTGGCTCTATGGTATGAATATGACGCGTGCGTACACCATCCAAGGTAAAAAGGT
CGGTTATCAAGGCGTGTTATCAGTCGGCCGAGTACAAACGCCATTGCTTGGGCTAGTGGTGCGCCGTGATAAAGAAATTG
CTAATTTTCAGTCTAAGCCTTTTTATGAAGTACTGGCACATTTGGCAACGGACAAGCAAGAAACCTTCAGCGCCAAGTGG
CAACCGAGTGAAGCTTGTCAGCCTTATATGGATGAAGAAGGACGAGTGTTAGCGAGAGGGTTAGCGCAAAATGTGGTGAG
TCGCATCAGCGATAAACCCGCCTTAGTCACTCAATTAGTGGCTAAGGATAAGAAACAGCATCCGCCTTTACCTTACAGTT
TATCAGCGTTGCAGATTGATGCGGCTAAACGGTTTGGTATGAGTGCTAAGGATGTGCTCGATACCTGCCAAAGTTTGTAT
GAACGTCATAAACTCATTACTTACCCACGCTCCGACAGTCGTTATTTACCCGTTGAGCAACATAATCTTGCGCCTTCGGT
ACTTAAGGCGATAAGTGACGGTGCTGCTGAGTTATTGCAAGGTGCTGATGCACCCGATCCCCGTCTTAAATCTAAAGCAT
GGGATGATAAAAAAGTGGACGCCCACCACGCAATCGTTCCAACAGAGAAGACGGCTAACTTATCTAGCTTGAGTCAGCGT
GAGAGGCAGTTATATCTTCATATTGCCCGCCAGTACTTAGCCCAGTTTTATCCGGCTTATTGTTACAGTGAAACCACAGT
GCAAGTGACAATCGAGGGTGGCTTATTTAATACCAAGGCGCGTCAGGATAAATCCCTCGGCTGGAAGCAACTCTTTGCCC
GCCAAGAGCCAAATGGCAGTAAGGCGAGTGGCAACAAGTCGACGGAAGAAAGCGCAGGTAAGGATGATGAGGAGCATGAC
GAGTTTATTGGCCAATTACCGCCTTTGAAATTGGGGCAAGCTTTGCATTGTACTCGAGGCGAATTGCTTGAAAAAAATAC
TCAGCCACCAAAGGCCTTTACCGATGCGACCTTACTCAGTGCGATGACGGGCATTAGTCGTTATGTCACTGACCCTGAGA
TCCGTAAAATTCTTAAAGAAACCGATGGATTAGGTACTGAGGCCACCCGTGCAGGTATTATCGAATTGCTATTCAAACGC
GGTTTTTTACAACGCCTAGGTAAATCTATTGTCTCGACAGACGTGGGGAAAGGATTGATTAACAGCTTGCCTTTGAGCGC
CACTACGCCGGATATGACGGCGCTTTGGGAAGCGAGCTTGAACGGCATTTGTCATAAAGAAACCTCCTATCAAGGATTTA
TGCAGCCGTTGCTCGGCACCTTATCCACACTTATCCAAAATGCGGGAGCACAGTTGCCAACTGCACTTAATGGATTAAAA
GGGCAAGGTTATCGTAAAACGGCAAGTCATAAATCCGGTTATCGTAAGTCGTATTCTAAAAAATCAAGTTCAGCGAAAGG
CATAGGTACAACAAAAAACGATGTTTCCAACAAATATGGTTCCCAAAAGACCAGTTCCAAAAGTTCGGTAACTCGATCAA
CCAGTCGTTCAGCTTCTAATAGCCGTAAATCTACAGCAGCAGTGTAA

Upstream 100 bases:

>100_bases
CAATTTAATAATTGCTTTCCTGTCAGGCTTGCTGTGATTTGCACTTAGAAGGACTTTGAGCTAGATTGTACTGTAATTAT
ATCCAGTGTTTGGTGTTTGT

Downstream 100 bases:

>100_bases
TGACACACTTGTTGCATACATGTCGTTATTGTCTTTTGATGTAATAACGACATTTAATTTATCTAACGGTATTTAACAAC
CGATAATCACGATATTTCGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKAWKFEHLPIVPDKWQLTPKAA
TRSQLTVLKRLVKQASMLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDKEIANFQSKPFYEVLAHLATDKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQHPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHNLAPSVLKAISDGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHIARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEESAGKDDEEHD
EFIGQLPPLKLGQALHCTRGELLEKNTQPPKAFTDATLLSAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPLSATTPDMTALWEASLNGICHKETSYQGFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTASHKSGYRKSYSKKSSSAKGIGTTKNDVSNKYGSQKTSSKSSVTRSTSRSASNSRKSTAAV

Sequences:

>Translated_708_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKAWKFEHLPIVPDKWQLTPKAA
TRSQLTVLKRLVKQASMLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDKEIANFQSKPFYEVLAHLATDKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQHPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHNLAPSVLKAISDGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHIARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEESAGKDDEEHD
EFIGQLPPLKLGQALHCTRGELLEKNTQPPKAFTDATLLSAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPLSATTPDMTALWEASLNGICHKETSYQGFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTASHKSGYRKSYSKKSSSAKGIGTTKNDVSNKYGSQKTSSKSSVTRSTSRSASNSRKSTAAV
>Mature_708_residues
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKAWKFEHLPIVPDKWQLTPKAA
TRSQLTVLKRLVKQASMLVNAGDPDREGQLLVDEVIAYLGVTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLST
SALARSRADWLYGMNMTRAYTIQGKKVGYQGVLSVGRVQTPLLGLVVRRDKEIANFQSKPFYEVLAHLATDKQETFSAKW
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQHPPLPYSLSALQIDAAKRFGMSAKDVLDTCQSLY
ERHKLITYPRSDSRYLPVEQHNLAPSVLKAISDGAAELLQGADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQR
ERQLYLHIARQYLAQFYPAYCYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEESAGKDDEEHD
EFIGQLPPLKLGQALHCTRGELLEKNTQPPKAFTDATLLSAMTGISRYVTDPEIRKILKETDGLGTEATRAGIIELLFKR
GFLQRLGKSIVSTDVGKGLINSLPLSATTPDMTALWEASLNGICHKETSYQGFMQPLLGTLSTLIQNAGAQLPTALNGLK
GQGYRKTASHKSGYRKSYSKKSSSAKGIGTTKNDVSNKYGSQKTSSKSSVTRSTSRSASNSRKSTAAV

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=650, Percent_Identity=23.5384615384615, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1788061, Length=671, Percent_Identity=56.6318926974665, Blast_Score=748, Evalue=0.0,
Organism=Escherichia coli, GI1787529, Length=573, Percent_Identity=24.4328097731239, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=573, Percent_Identity=22.5130890052356, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585251, Length=538, Percent_Identity=25.4646840148699, Blast_Score=102, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 77952; Mature: 77952

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKA
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDKWQLTPKAATRSQLTVLKRLVKQASMLVNAGDPDREGQLLVDEVIAYLG
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLVVRRDKEIANFQSKPFYEVLAHLATDKQETFSAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCHHHHCCCC
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQHPPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPVEQHNLAPSVLKAISDGAAELLQ
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
GADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQRERQLYLHIARQYLAQFYPAY
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEESAGKDDEEHD
HCCCCEEEEEEECCEECCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHH
EFIGQLPPLKLGQALHCTRGELLEKNTQPPKAFTDATLLSAMTGISRYVTDPEIRKILKE
HHHHCCCCHHHCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TDGLGTEATRAGIIELLFKRGFLQRLGKSIVSTDVGKGLINSLPLSATTPDMTALWEASL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
NGICHKETSYQGFMQPLLGTLSTLIQNAGAQLPTALNGLKGQGYRKTASHKSGYRKSYSK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
KSSSAKGIGTTKNDVSNKYGSQKTSSKSSVTRSTSRSASNSRKSTAAV
CCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MILYIAEKPSLGRAIADVLPKPHKKGDGFIEAANGDCVSWCVGHLLEQAEPDAYNPEFKA
CEEEEECCCCHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCC
WKFEHLPIVPDKWQLTPKAATRSQLTVLKRLVKQASMLVNAGDPDREGQLLVDEVIAYLG
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHC
VTGDKLHQTQRLLISDLNPQAVKRALTQLRSNREFIPLSTSALARSRADWLYGMNMTRAY
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCCCCEEE
TIQGKKVGYQGVLSVGRVQTPLLGLVVRRDKEIANFQSKPFYEVLAHLATDKQETFSAKW
EECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHCCCHHHHCCCC
QPSEACQPYMDEEGRVLARGLAQNVVSRISDKPALVTQLVAKDKKQHPPLPYSLSALQID
CCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHEEH
AAKRFGMSAKDVLDTCQSLYERHKLITYPRSDSRYLPVEQHNLAPSVLKAISDGAAELLQ
HHHHHCCCHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHH
GADAPDPRLKSKAWDDKKVDAHHAIVPTEKTANLSSLSQRERQLYLHIARQYLAQFYPAY
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
CYSETTVQVTIEGGLFNTKARQDKSLGWKQLFARQEPNGSKASGNKSTEESAGKDDEEHD
HCCCCEEEEEEECCEECCCHHHHHCCCHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHH
EFIGQLPPLKLGQALHCTRGELLEKNTQPPKAFTDATLLSAMTGISRYVTDPEIRKILKE
HHHHCCCCHHHCCHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TDGLGTEATRAGIIELLFKRGFLQRLGKSIVSTDVGKGLINSLPLSATTPDMTALWEASL
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
NGICHKETSYQGFMQPLLGTLSTLIQNAGAQLPTALNGLKGQGYRKTASHKSGYRKSYSK
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHH
KSSSAKGIGTTKNDVSNKYGSQKTSSKSSVTRSTSRSASNSRKSTAAV
CCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2553698; 9097039; 9278503; 10574789 [H]