Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 120598389

GI number: 120598389

Start: 1790754

End: 1792268

Strand: Direct

Name: purF [H]

Synonym: Sputw3181_1571

Alternate gene names: 120598389

Gene position: 1790754-1792268 (Clockwise)

Preceding gene: 120598388

Following gene: 120598390

Centisome position: 38.03

GC content: 46.8

Gene sequence:

>1515_bases
ATGTGTGGTATCGTCGGAATAGTTGGCCAATCATCGGTTAATCAGACCATTTATGATGCACTGACCGTGCTTCAGCACAG
AGGTCAGGATGCGGCAGGTATTGTGACCGTTGATCGTGGTGCTTTCAGACTACGTAAAGCCAACGGTCTGGTAAAAGATG
TATTTGAAGTCAAGCATATGCAGCGCTTACAGGGCAATGCAGGGATTGGCCATGTACGTTATCCAACTGCGGGCAGTTCA
AGCGCATCAGAAGCGCAGCCTTTCTATGTTAACTCGCCATTTGGGATTTCATTAGCCCACAACGGTAACTTAACCAACAC
AGTCGAATTAGCCGAAGGGCTGATTAAAAAACGTCGTCATGTGAATACCACCTCAGATTCTGAAGTGTTATTAAATCTCT
TGGCTGATGAGCTGCAAAAAACCACGAGTTTGACTCTGACGTCGGAAGAAGTGTTCGATGCGGTCGCAAATGTGCACCAG
CAAGCCCGTGGTGCCTACGCTGTGGTTGCCATGATTATTGGTCAAGGCTTAGTCGCGTTTCGTGATCCATTTGGTATTCG
TCCTTTGGTGTTAGGTAAGCACGAAACTGCTACTGGCACTGAATACATGGTCGCTTCAGAAAGTGTGGCACTCGATGCCG
TTGGCTTTGAAGTGATGCGTGATGTCGCGCCGGGTGAAGCGATTTATGTGTCAATTGACGGCCAGCTGTATACCCGTCAA
TGCGCGGCAGAACCGAGCTATGCGCCTTGTATCTTCGAATTCGTTTATTTTGCCCGTCCAGATTCGACTATCGACAAAGT
GTCTGTGTATGCAAGTCGCGTCAACATGGGCGCTAAGCTTGGCGAGAAGATTAAGAAGGAATGGTACGATCACGATATCG
ATGTGGTGATCCCTATTCCTGAAACCTCCTGCGATATCGCGCTGGAAATTGCCCGTTGCATGGACTTACCGTACCGCCAA
GGTTTTGTGAAAAACCGTTATATTGGCCGTACCTTCATTATGCCTGGTCAACAGGAACGTAAGAAATCAGTGCGCCGTAA
ACTCAATGCAATCAACACTGAGTTTAAAGGTAAAAATGTACTGTTAGTGGATGACTCTATCGTTCGCGGGACGACCTCTG
AGCAGATCATCGAAATGGCTCGTGAAGCGGGTGCTAAGAAAGTGTATTTTGCTTCTGCTGCGCCAGAGATTCGTTTCCCG
AACGTGTATGGTATCGACATGCCGACCTCGAATGAGCTGATCGCCCATGGACGTGATGCCGATGAAATCGCTAAACTGAT
TGGTGCAGATGGTATTATTTTCCAAAATCTGTCTGATCTAGTTGAAGCGGTGAGAATGGAAAACCCTGAGATTAAACGCT
TCGAGACCTCAGTATTCGATGGTCACTATATTACCAATGACGTTGATCAAGCGTATTTAGATCACTTAACGCAACTGCGT
AACGATGATGCAAAAGCGGATCGTAATAAAGATATTGGTACTAACTTAGAGTTGCATAACGTCTGTCACCCTTAA

Upstream 100 bases:

>100_bases
TATAAACCTAAGTCAAAATGTCTATTCAACGTCATTTTTCTTTTTGCTTATATTTCAACATGAAAGGACATAGAACATCT
TACAATGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
CGGCAAGTGTTAGGCAGATCATGGGCCAGTGATGATATCACTGGCTTTTTTTATGGTTCTTTAAGCATATTGATTATACC
AATCGTATTAAAAACCAGTG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLSDLVEAVRMENPEIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Sequences:

>Translated_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLSDLVEAVRMENPEIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP
>Mature_504_residues
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHMQRLQGNAGIGHVRYPTAGSS
SASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRHVNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQ
QARGAYAVVAMIIGQGLVAFRDPFGIRPLVLGKHETATGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIPETSCDIALEIARCMDLPYRQ
GFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFP
NVYGIDMPTSNELIAHGRDADEIAKLIGADGIIFQNLSDLVEAVRMENPEIKRFETSVFDGHYITNDVDQAYLDHLTQLR
NDDAKADRNKDIGTNLELHNVCHP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=469, Percent_Identity=36.2473347547974, Blast_Score=268, Evalue=6e-72,
Organism=Escherichia coli, GI1788651, Length=503, Percent_Identity=73.1610337972167, Blast_Score=758, Evalue=0.0,
Organism=Escherichia coli, GI1790167, Length=173, Percent_Identity=33.5260115606936, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=462, Percent_Identity=32.6839826839827, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6323958, Length=510, Percent_Identity=49.8039215686275, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24659598, Length=499, Percent_Identity=34.6693386773547, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24659604, Length=475, Percent_Identity=35.3684210526316, Blast_Score=258, Evalue=5e-69,
Organism=Drosophila melanogaster, GI28573187, Length=470, Percent_Identity=34.0425531914894, Blast_Score=253, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55573; Mature: 55573

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETATGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHH
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHCCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQNLSDLVEAVRMENPEIKRFETSVFDGHYITNDVDQAYLDHLTQLR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGIVGIVGQSSVNQTIYDALTVLQHRGQDAAGIVTVDRGAFRLRKANGLVKDVFEVKHM
CCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEHHHCCHHHHHHHHHHH
QRLQGNAGIGHVRYPTAGSSSASEAQPFYVNSPFGISLAHNGNLTNTVELAEGLIKKRRH
HHHCCCCCCCEEECCCCCCCCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHC
VNTTSDSEVLLNLLADELQKTTSLTLTSEEVFDAVANVHQQARGAYAVVAMIIGQGLVAF
CCCCCHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEE
RDPFGIRPLVLGKHETATGTEYMVASESVALDAVGFEVMRDVAPGEAIYVSIDGQLYTRQ
CCCCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEHH
CAAEPSYAPCIFEFVYFARPDSTIDKVSVYASRVNMGAKLGEKIKKEWYDHDIDVVIPIP
HCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETSCDIALEIARCMDLPYRQGFVKNRYIGRTFIMPGQQERKKSVRRKLNAINTEFKGKNV
CCCCHHHHHHHHHHCCHHHCCCHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
LLVDDSIVRGTTSEQIIEMAREAGAKKVYFASAAPEIRFPNVYGIDMPTSNELIAHGRDA
EEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCCEECCCCH
DEIAKLIGADGIIFQNLSDLVEAVRMENPEIKRFETSVFDGHYITNDVDQAYLDHLTQLR
HHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHC
NDDAKADRNKDIGTNLELHNVCHP
CCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3047685; 6443594; 6277938; 9205837; 9278503; 3040734; 7037784; 9333323; 9514258 [H]