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The map label for this gene is folC [H]

Identifier: 120598386

GI number: 120598386

Start: 1787985

End: 1789256

Strand: Direct

Name: folC [H]

Synonym: Sputw3181_1568

Alternate gene names: 120598386

Gene position: 1787985-1789256 (Clockwise)

Preceding gene: 120598385

Following gene: 120598387

Centisome position: 37.97

GC content: 47.41

Gene sequence:

>1272_bases
ATGCTAACAGCCCCCGCGTCAAATGCAGCACTGGAACAGTGGTTGGATTATTTGTTGTCCATTCATCCAAGTGAAATCGA
TATGGGGTTAACCCGTGTCGCTAAGGTCGCGCAAATTCTCAATCTTTTGGATTTAGGTCAAACCAAAATAGTGACAGTGG
CGGGAACGAACGGTAAAGGCACCACTTGTGCCATGCTCGAAAATATTCTGCGTTTATCTGGCTTAACCGTCGGTGTATAT
AGCTCGCCGCACATGCTGAAGTACAATGAACGAGTGCGAATCAATGGTAGCGATGCCAGTGATGGTGCTTTTGTTGCGGC
ATTTGAGCAAATTGAAGCCGCGCGCGGCGATATTTCTCTGACCTTTTTTGAGTACGCTACGCTTGCAGGCTTGATACTCT
TTAAAGCCGCTCAGCTTGACGTGGTTATCTTAGAAGTCGGTTTAGGTGGGCGGTTAGATGCCACCAATATTATCGATGCC
GATATCAGTGTGGTCACGTCAGTCGACTTAGATCATGAAAGTTATTTAGGTAATACCCGTGAGCTTGTGGGCCGTGAAAA
GGCAGGTATTTTCCGCTGTGGTAAACCTGCGATTGTGGGGGAGCCTAACTTGCCACACACAGTTAATGAAGTCGCCAATG
AGGTGGGCGCAATCTTATATCGCGTTGGCAACGAGTTCAGTTATCAAGTCCATGGCAAGCTGTGGGATTTTAAAGGCCAA
GTACTCAAACTGCACGATTTAGCGTTACCTGCTTTGCCATTACCTAATGCGGCGACTGTATTGGCTGTGATTGAACAGGG
GTGGCCGAATATCGCGCCTGCTGTTATTGCAGAGGGGATTAACTCGGCTCGCTTAACGGGTCGACTGGAACGGGTGAATG
AGCAACCGTTAGTGCTACTCGATGTGGCTCATAATCCCCATGCGGCGCGGTTTTTGGTACAACAGTTAGCATCAATGGTT
GTGGGTAAGCGCGTCTTTGCCCTTTGCGGTATGTTGAAGGATAAAGATATCGCGGGTGTATTACCTGTGGTTGATCCTTT
AGTGGATACTTGGTATTTAGTCAGCTTGCATGGTGAACGTGGTGCGAGTGCAAGCCAATTACGTAAAACCTTAGCCGCAG
AGGCTAATGCGTTTGAATTTGAGGGAATAGATGCCGCTTGGTCGGCATTAAAAGCGCAAATTAGCCCTGATGATGTGGTA
ATTGTGTTTGGCTCCTTTTACACTGTGGCAGGATTTAAATCTCTATTTAAGCAGGATGTCAGTTTTGTCTAG

Upstream 100 bases:

>100_bases
CGCGCTTGGGCCATTATTTATGTTGGATTAATGTCGTTGGGATAATTAGACCAATATCTGAATATGATTATCGAGCGTGA
TTAAAGGAAGGAAATAACAG

Downstream 100 bases:

>100_bases
CCAGTTTCATAATCGTTTAGTCGGCACTGTGGTGCTGGTCGCCCTCGGGGTGATATTTTTACCGGATATTTTGGACGGCA
AAAAAGAGCGTCAAGAAGAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLTAPASNAALEQWLDYLLSIHPSEIDMGLTRVAKVAQILNLLDLGQTKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDGAFVAAFEQIEAARGDISLTFFEYATLAGLILFKAAQLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRCGKPAIVGEPNLPHTVNEVANEVGAILYRVGNEFSYQVHGKLWDFKGQ
VLKLHDLALPALPLPNAATVLAVIEQGWPNIAPAVIAEGINSARLTGRLERVNEQPLVLLDVAHNPHAARFLVQQLASMV
VGKRVFALCGMLKDKDIAGVLPVVDPLVDTWYLVSLHGERGASASQLRKTLAAEANAFEFEGIDAAWSALKAQISPDDVV
IVFGSFYTVAGFKSLFKQDVSFV

Sequences:

>Translated_423_residues
MLTAPASNAALEQWLDYLLSIHPSEIDMGLTRVAKVAQILNLLDLGQTKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDGAFVAAFEQIEAARGDISLTFFEYATLAGLILFKAAQLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRCGKPAIVGEPNLPHTVNEVANEVGAILYRVGNEFSYQVHGKLWDFKGQ
VLKLHDLALPALPLPNAATVLAVIEQGWPNIAPAVIAEGINSARLTGRLERVNEQPLVLLDVAHNPHAARFLVQQLASMV
VGKRVFALCGMLKDKDIAGVLPVVDPLVDTWYLVSLHGERGASASQLRKTLAAEANAFEFEGIDAAWSALKAQISPDDVV
IVFGSFYTVAGFKSLFKQDVSFV
>Mature_423_residues
MLTAPASNAALEQWLDYLLSIHPSEIDMGLTRVAKVAQILNLLDLGQTKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDGAFVAAFEQIEAARGDISLTFFEYATLAGLILFKAAQLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRCGKPAIVGEPNLPHTVNEVANEVGAILYRVGNEFSYQVHGKLWDFKGQ
VLKLHDLALPALPLPNAATVLAVIEQGWPNIAPAVIAEGINSARLTGRLERVNEQPLVLLDVAHNPHAARFLVQQLASMV
VGKRVFALCGMLKDKDIAGVLPVVDPLVDTWYLVSLHGERGASASQLRKTLAAEANAFEFEGIDAAWSALKAQISPDDVV
IVFGSFYTVAGFKSLFKQDVSFV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=191, Percent_Identity=37.696335078534, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI66932990, Length=191, Percent_Identity=37.696335078534, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=52.2167487684729, Blast_Score=394, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71984923, Length=450, Percent_Identity=26, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553148, Length=450, Percent_Identity=26, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17553150, Length=450, Percent_Identity=26, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323760, Length=318, Percent_Identity=30.188679245283, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324815, Length=182, Percent_Identity=33.5164835164835, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24641571, Length=326, Percent_Identity=30.0613496932515, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24581568, Length=191, Percent_Identity=34.5549738219895, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45497; Mature: 45497

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAPASNAALEQWLDYLLSIHPSEIDMGLTRVAKVAQILNLLDLGQTKIVTVAGTNGKG
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGSDASDGAFVAAFEQIEAARGDISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
TFFEYATLAGLILFKAAQLDVVILEVGLGGRLDATNIIDADISVVTSVDLDHESYLGNTR
EHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHCCCHH
ELVGREKAGIFRCGKPAIVGEPNLPHTVNEVANEVGAILYRVGNEFSYQVHGKLWDFKGQ
HHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCC
VLKLHDLALPALPLPNAATVLAVIEQGWPNIAPAVIAEGINSARLTGRLERVNEQPLVLL
EEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCEEEE
DVAHNPHAARFLVQQLASMVVGKRVFALCGMLKDKDIAGVLPVVDPLVDTWYLVSLHGER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCC
GASASQLRKTLAAEANAFEFEGIDAAWSALKAQISPDDVVIVFGSFYTVAGFKSLFKQDV
CCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHC
SFV
CCC
>Mature Secondary Structure
MLTAPASNAALEQWLDYLLSIHPSEIDMGLTRVAKVAQILNLLDLGQTKIVTVAGTNGKG
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
TTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGSDASDGAFVAAFEQIEAARGDISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCCHHHHHHHHHHHCCCCEEE
TFFEYATLAGLILFKAAQLDVVILEVGLGGRLDATNIIDADISVVTSVDLDHESYLGNTR
EHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHCCCHH
ELVGREKAGIFRCGKPAIVGEPNLPHTVNEVANEVGAILYRVGNEFSYQVHGKLWDFKGQ
HHHCCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCC
VLKLHDLALPALPLPNAATVLAVIEQGWPNIAPAVIAEGINSARLTGRLERVNEQPLVLL
EEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCEEEE
DVAHNPHAARFLVQQLASMVVGKRVFALCGMLKDKDIAGVLPVVDPLVDTWYLVSLHGER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCEEEEEEECCC
GASASQLRKTLAAEANAFEFEGIDAAWSALKAQISPDDVVIVFGSFYTVAGFKSLFKQDV
CCCHHHHHHHHHHCCCCEEECCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHC
SFV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]