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The map label for this gene is truA

Identifier: 120598385

GI number: 120598385

Start: 1787130

End: 1787915

Strand: Direct

Name: truA

Synonym: Sputw3181_1567

Alternate gene names: 120598385

Gene position: 1787130-1787915 (Clockwise)

Preceding gene: 120598384

Following gene: 120598386

Centisome position: 37.96

GC content: 45.55

Gene sequence:

>786_bases
ATGCGAATTGCATTAGGTATTGAATATGATGGCAGTGGTTATTTTGGTTGGCAACGTCAGGCCGAAGTCGATTCAGTACA
AGGGCAATTAGAGCGTGCTTTATCGATAGTGGCAAATGAACCTATAGGGTTATTTTGTGCCGGACGTACCGATGCGGGTG
TGCATGCCACAGGCCAAGTGGTGCATTTTGATACGAATGCAATTCGAAATGAAGGCGCTTGGACTTTAGGGGTAAATGCT
AATTTACCTGATAATATTGCCGTACGTTGGGTCAAGGAAGTCGACGACAGTTTTCATGCGCGCTTTTCGGCCACCGCCAG
ACGCTACCGTTATGTGATTTATAATCACAGTTTTCGCCCTGGGATTTTACGCCATGGTGTAAGCCATTATCACGGTAATA
TTGATGCCGACAAAATGCACCAAGCGGCGCAGGTACTTTTGGGTGAGCAGGATTTTACCAGTTTTCGCGCGGTGCAGTGT
CAGTCGAAAACACCGTTTCGTAATGTACATAGTGTCAATGTTACGCGCCAAGGCATGTATGTGATTGTCGATATCGCCGC
CAATGCTTTCTTGCATCATATGGTGCGTAATATCGTCGGTTCATTGTTAGAAATCGGTCTAGGTAATCAGCCACTGACTT
GGATGGGTGATTTGCTCGCGTTAAAAGATAGAAACCAAGCCGCAGCAACGGCTAAACCTCATGGACTCTATCTAGTTGAT
GTGACTTATCCAGAACAATATCAATTGCCTAAGCTCGCGCTTGGGCCATTATTTATGTTGGATTAA

Upstream 100 bases:

>100_bases
TCGATTAACCGAGTTTCAATCTAACGGCGCTTCGGGCGCCGTTTTTTTTAAGGGATGCTGTGATAAAATGCGCCGCTAAA
TTCATTGTGAGGGTGTGTGG

Downstream 100 bases:

>100_bases
TGTCGTTGGGATAATTAGACCAATATCTGAATATGATTATCGAGCGTGATTAAAGGAAGGAAATAACAGATGCTAACAGC
CCCCGCGTCAAATGCAGCAC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRIALGIEYDGSGYFGWQRQAEVDSVQGQLERALSIVANEPIGLFCAGRTDAGVHATGQVVHFDTNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGNIDADKMHQAAQVLLGEQDFTSFRAVQC
QSKTPFRNVHSVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD

Sequences:

>Translated_261_residues
MRIALGIEYDGSGYFGWQRQAEVDSVQGQLERALSIVANEPIGLFCAGRTDAGVHATGQVVHFDTNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGNIDADKMHQAAQVLLGEQDFTSFRAVQC
QSKTPFRNVHSVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD
>Mature_261_residues
MRIALGIEYDGSGYFGWQRQAEVDSVQGQLERALSIVANEPIGLFCAGRTDAGVHATGQVVHFDTNAIRNEGAWTLGVNA
NLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRPGILRHGVSHYHGNIDADKMHQAAQVLLGEQDFTSFRAVQC
QSKTPFRNVHSVNVTRQGMYVIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
VTYPEQYQLPKLALGPLFMLD

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=26.5682656826568, Blast_Score=74, Evalue=9e-14,
Organism=Escherichia coli, GI1788657, Length=260, Percent_Identity=71.1538461538462, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI116007752, Length=306, Percent_Identity=28.4313725490196, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 29070; Mature: 29070

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALGIEYDGSGYFGWQRQAEVDSVQGQLERALSIVANEPIGLFCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCEE
VHFDTNAIRNEGAWTLGVNANLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRP
EEEECHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHEEEEEEEECCCCC
GILRHGVSHYHGNIDADKMHQAAQVLLGEQDFTSFRAVQCQSKTPFRNVHSVNVTRQGMY
HHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCE
VIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEE
VTYPEQYQLPKLALGPLFMLD
ECCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MRIALGIEYDGSGYFGWQRQAEVDSVQGQLERALSIVANEPIGLFCAGRTDAGVHATGQV
CEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEECCEE
VHFDTNAIRNEGAWTLGVNANLPDNIAVRWVKEVDDSFHARFSATARRYRYVIYNHSFRP
EEEECHHHCCCCCEEEEECCCCCCHHHHHHHHHHCHHHHHHHHHHHHEEEEEEEECCCCC
GILRHGVSHYHGNIDADKMHQAAQVLLGEQDFTSFRAVQCQSKTPFRNVHSVNVTRQGMY
HHHHHCHHHHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCE
VIVDIAANAFLHHMVRNIVGSLLEIGLGNQPLTWMGDLLALKDRNQAAATAKPHGLYLVD
EEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCCCCCCCCCCEEEEE
VTYPEQYQLPKLALGPLFMLD
ECCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA