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The map label for this gene is pdxB [H]

Identifier: 120598382

GI number: 120598382

Start: 1781407

End: 1782537

Strand: Direct

Name: pdxB [H]

Synonym: Sputw3181_1564

Alternate gene names: 120598382

Gene position: 1781407-1782537 (Clockwise)

Preceding gene: 120598381

Following gene: 120598383

Centisome position: 37.83

GC content: 46.95

Gene sequence:

>1131_bases
ATGAAGATTTTAGTCGATGAAAATATGCCCTTTGTTGAACCGTTATTTGGCGATCTCGGGGAAATTATTCCTGTTAATGG
CCGCACGTTAACCGTAGCGCAAGTGCGCGATGCCGATGTGTTATTAGTGCGCTCTGTCACCAAAGTGAATGCCGAGTTGC
TCTCGGACAATCATCAACTGAAGTTTGTCGGCAGTGCCACTATAGGCACAGATCATGTGGATTTAGCCTACCTTGGCGAG
CGTAATATTCCCTTCTCTAATGCGCCAGGTTGCAATGCGACTGCGGTTGGGGAGTTTGCTTTTATCGCTATGCTGGAGTT
AGCACAGCGGTTTGATTCGCCGCTTAAGGGCAAGGTCGTCGGCATAGTGGGCGCGGGCAATACTGGTACTGCAACGGCTA
AATGCTTGCAAGCCTATGGTATTAAGGTGTTGCTTAATGATCCCATCAAAGAGAAACAGGGCGATCCGCGCAGTTTTGTG
AGCTTAGAAACCATTATGGCGCAGGCCGATATCATCAGTTTACATGTGCCAATCACGCGCACGGGCGAGCATAAAACCAA
ACATTTATTAGATGAGGCGCGCTTAACGGCGCTCAAACCCAATACATGGTTAGTGAATTGCTGTCGTGGCGATGTGATAG
ATAACAAAGCACTTGTCAGAGTTAAACGCCAGCGTGATGATCTCAGACTTGTACTCGATGTGTGGGAAGGCGAGCCAACA
CCTATGCCAGAACTCGTGCCACTGGCTGAATTTGCCACGCCGCACATTGCAGGTTACAGCCTAGAAGGTAAGGCGCGCGG
CACTTTTATGCTTTACCAAAAACTGTGTCAGTTACTTAATATTGCTGCCGATAAAAGCCTGCTGGATCTCTTGCCAAGCT
TTAATATTAAGGCAGTTGAACTGGCGACAGCACCTGACGAAAAGGCACTCTTACAGTTGGCACGTTTTGTTTATGATTTG
CGCGATGATGACAATATGTTCCGAAATATATTCTTAAATGAGAATGGCTTTGACACTATGAGAAAAAATCACCGACATCG
ACGTGAATTTAGTGCATTGGCGCTGGCTTATGACCGTCAACTTGAGGTAGATTGGTTATCCAATTTAGGGTTTTCAGGAG
TCGGTCAGTAG

Upstream 100 bases:

>100_bases
TTGTAAACCTGTTGCATCGACAAGGATTTATCCCATTAGTCTTGTTAGAATACCGAGCATCTTGATGCCTTCCATTCCCA
TTTTTTCAAATCAGGTGCCC

Downstream 100 bases:

>100_bases
CTATGTCACAGGAATTTAATGTTGTCGTTTTAGGTGCATCGGGCGCAGTGGGTCAAACTATGATTGAGATCCTCGAAGAG
CGTAACTTCCCGGTTGCCAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKILVDENMPFVEPLFGDLGEIIPVNGRTLTVAQVRDADVLLVRSVTKVNAELLSDNHQLKFVGSATIGTDHVDLAYLGE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFDSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKEKQGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKHLLDEARLTALKPNTWLVNCCRGDVIDNKALVRVKRQRDDLRLVLDVWEGEPT
PMPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNIAADKSLLDLLPSFNIKAVELATAPDEKALLQLARFVYDL
RDDDNMFRNIFLNENGFDTMRKNHRHRREFSALALAYDRQLEVDWLSNLGFSGVGQ

Sequences:

>Translated_376_residues
MKILVDENMPFVEPLFGDLGEIIPVNGRTLTVAQVRDADVLLVRSVTKVNAELLSDNHQLKFVGSATIGTDHVDLAYLGE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFDSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKEKQGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKHLLDEARLTALKPNTWLVNCCRGDVIDNKALVRVKRQRDDLRLVLDVWEGEPT
PMPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNIAADKSLLDLLPSFNIKAVELATAPDEKALLQLARFVYDL
RDDDNMFRNIFLNENGFDTMRKNHRHRREFSALALAYDRQLEVDWLSNLGFSGVGQ
>Mature_376_residues
MKILVDENMPFVEPLFGDLGEIIPVNGRTLTVAQVRDADVLLVRSVTKVNAELLSDNHQLKFVGSATIGTDHVDLAYLGE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFDSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKEKQGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKHLLDEARLTALKPNTWLVNCCRGDVIDNKALVRVKRQRDDLRLVLDVWEGEPT
PMPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNIAADKSLLDLLPSFNIKAVELATAPDEKALLQLARFVYDL
RDDDNMFRNIFLNENGFDTMRKNHRHRREFSALALAYDRQLEVDWLSNLGFSGVGQ

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=311, Percent_Identity=27.3311897106109, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI145580578, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4557499, Length=226, Percent_Identity=26.9911504424779, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI145580575, Length=155, Percent_Identity=31.6129032258064, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI61743967, Length=225, Percent_Identity=24.8888888888889, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4557497, Length=225, Percent_Identity=24.8888888888889, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1788660, Length=374, Percent_Identity=45.7219251336898, Blast_Score=290, Evalue=8e-80,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=28.957528957529, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787645, Length=201, Percent_Identity=27.363184079602, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=24.8, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=252, Percent_Identity=28.968253968254, Blast_Score=104, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=318, Percent_Identity=24.8427672955975, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=318, Percent_Identity=25.1572327044025, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=227, Percent_Identity=25.5506607929515, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324964, Length=281, Percent_Identity=22.7758007117438, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19921140, Length=259, Percent_Identity=27.4131274131274, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24646446, Length=230, Percent_Identity=28.2608695652174, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646448, Length=230, Percent_Identity=28.2608695652174, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646452, Length=230, Percent_Identity=28.2608695652174, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24646450, Length=230, Percent_Identity=28.2608695652174, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI62472511, Length=230, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574286, Length=268, Percent_Identity=25.3731343283582, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41664; Mature: 41664

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPFVEPLFGDLGEIIPVNGRTLTVAQVRDADVLLVRSVTKVNAELLSDNHQL
CEEEECCCCCHHHHHHHCCCCEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHCCCCEE
KFVGSATIGTDHVDLAYLGERNIPFSNAPGCNATAVGEFAFIAMLELAQRFDSPLKGKVV
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTATAKCLQAYGIKVLLNDPIKEKQGDPRSFVSLETIMAQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCEEEEEECEEC
TGEHKTKHLLDEARLTALKPNTWLVNCCRGDVIDNKALVRVKRQRDDLRLVLDVWEGEPT
CCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHEEEHHCCCCEEEEEEEECCCCC
PMPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNIAADKSLLDLLPSFNIKAVE
CCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEE
LATAPDEKALLQLARFVYDLRDDDNMFRNIFLNENGFDTMRKNHRHRREFSALALAYDRQ
EECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
LEVDWLSNLGFSGVGQ
CCHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPFVEPLFGDLGEIIPVNGRTLTVAQVRDADVLLVRSVTKVNAELLSDNHQL
CEEEECCCCCHHHHHHHCCCCEEECCCCEEEEEEECCCCEEEEHHHHHHHHHHHCCCCEE
KFVGSATIGTDHVDLAYLGERNIPFSNAPGCNATAVGEFAFIAMLELAQRFDSPLKGKVV
EEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTATAKCLQAYGIKVLLNDPIKEKQGDPRSFVSLETIMAQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCHHHCCCCCHHHHHHHHHHHHCCEEEEEECEEC
TGEHKTKHLLDEARLTALKPNTWLVNCCRGDVIDNKALVRVKRQRDDLRLVLDVWEGEPT
CCCHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCHHHEEEHHCCCCEEEEEEEECCCCC
PMPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNIAADKSLLDLLPSFNIKAVE
CCCCHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEE
LATAPDEKALLQLARFVYDLRDDDNMFRNIFLNENGFDTMRKNHRHRREFSALALAYDRQ
EECCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
LEVDWLSNLGFSGVGQ
CCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA