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The map label for this gene is kinB [H]

Identifier: 120598301

GI number: 120598301

Start: 1672265

End: 1673614

Strand: Direct

Name: kinB [H]

Synonym: Sputw3181_1482

Alternate gene names: 120598301

Gene position: 1672265-1673614 (Clockwise)

Preceding gene: 120598298

Following gene: 120598302

Centisome position: 35.52

GC content: 40.74

Gene sequence:

>1350_bases
ATGACACGCATGTTTGACAGGATTTTATTTGATGTTAGTCAGTCCCCTTTGATTGATAAAGGAGACTTAACTGTGGCTGC
GCAGTTGATTGTATCTTCTGTTTGTAAAGGGCTGAATGTTAAGCGGGTAGGTATTTGGTTATTGAGTGAAGATAGGCAAT
CGATATCGTGTTTTTATCTGAGCCATCATCCCCAATTAGAGCAAACCTTGACACTGGCTCGTGTGCAATATCCCCATTAT
TTTGACTTATTAGATACCGAGCGAACGATAGTGCTAGATCATAGGCAACTGGATTTTACCACTCAAGAATTAGCGTCGAC
TTATTTCAACGCTAACAACATTACTGCCATATTAGCTAGCCCAATACGACACGATGGAAAAATGGTAGGGATTATTTGTG
TTGAGCATCAAGGCGAGCCTCGCGAATGGATGCCAGATGACAAAGCGTTTGTGTCGACTTTGGCGGAAATATTTGCCCGA
TCGATAAGCGCAAGGGACATTCTAGATTATCAACGGCAGTTAAAAGTGGTCAATGCAGATCTTGAACTGCGGACACAGGA
GCTAGAAGCCGCTTTAGCTCATTTAAGCGCGACGCAAAATCATTTGATTGAAAAAGAAAAAATGGCAGCATTAGGCAGTT
TAGTGGCAGGGGTTGCCCATGAAGTGAATACCCCTCTAGGGATAGCGGTGACGTCTGTGACTCATTGTTTAGATGAACTT
GCCTTACTTAAGAGATTCTTTGAATCTAATGAACTCGATCAGGAAAAATTTTTAAATTTTATGACTACGATGCAAGACGG
CTTGTCTCTTGTTGATCGTAATCTTGCGCGGGCAGCAGAGTTAGTGCACAACTTTAAGCGAACGGCTGCGGATCAATCAA
TTTGGGAGCGGGAACGATTTAATTTAAAAGCGTATATTTTGCAGGTTTTTAGCTCATTAAAACCGTTAATGCGTAAAAAA
AGTATTAGCTTAACGATTAATGTTGATGACGGTATTTATCTTTATTCTTACCCTGGGGCGATTGCTCAGATTTTTACTAA
TTTAGTGTCTAATAGTTTTCGCCATGGTTTCCCTGATAATTTTACGGGGGAGAAACAGATCATCGTTGAGGCAGATAAAA
CGAGTTCTACTATCAATATCATCTATCAGGATAGTGGTGTTGGAATGTCTGATGATATTAAAGCAAGAGCCTTTGAACCT
TTTTTTACGACAGCGAAGCAATCGGGTGGTACAGGTTTAGGGATGTCTATTATTCATAATCTTGTCACTCAAAAACTTAA
TGGCCGTATCTCGCTTATTTCGGCTCCAAACCAAGGTGTAAAAATCGAAATGCAGCTCCCAGAGGAGTAA

Upstream 100 bases:

>100_bases
GTTTTTAAAAATGTTAATATTTGGCGAATTTGACCCGTCATACCTAAGCAAATTAGTAATAAAGAGACTTACCCTACTAG
TGCTAACAGGATGAGCAATC

Downstream 100 bases:

>100_bases
AGTTAAAGCACTTATTGTAAGATCCCTCGCTATTTCTATGTAGGCATAAATTCAGAACCTTTGTTATTCACTCGAAGGCT
GGAATCTAGCGCCTTATGTC

Product: GAF sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTRMFDRILFDVSQSPLIDKGDLTVAAQLIVSSVCKGLNVKRVGIWLLSEDRQSISCFYLSHHPQLEQTLTLARVQYPHY
FDLLDTERTIVLDHRQLDFTTQELASTYFNANNITAILASPIRHDGKMVGIICVEHQGEPREWMPDDKAFVSTLAEIFAR
SISARDILDYQRQLKVVNADLELRTQELEAALAHLSATQNHLIEKEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCLDEL
ALLKRFFESNELDQEKFLNFMTTMQDGLSLVDRNLARAAELVHNFKRTAADQSIWERERFNLKAYILQVFSSLKPLMRKK
SISLTINVDDGIYLYSYPGAIAQIFTNLVSNSFRHGFPDNFTGEKQIIVEADKTSSTINIIYQDSGVGMSDDIKARAFEP
FFTTAKQSGGTGLGMSIIHNLVTQKLNGRISLISAPNQGVKIEMQLPEE

Sequences:

>Translated_449_residues
MTRMFDRILFDVSQSPLIDKGDLTVAAQLIVSSVCKGLNVKRVGIWLLSEDRQSISCFYLSHHPQLEQTLTLARVQYPHY
FDLLDTERTIVLDHRQLDFTTQELASTYFNANNITAILASPIRHDGKMVGIICVEHQGEPREWMPDDKAFVSTLAEIFAR
SISARDILDYQRQLKVVNADLELRTQELEAALAHLSATQNHLIEKEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCLDEL
ALLKRFFESNELDQEKFLNFMTTMQDGLSLVDRNLARAAELVHNFKRTAADQSIWERERFNLKAYILQVFSSLKPLMRKK
SISLTINVDDGIYLYSYPGAIAQIFTNLVSNSFRHGFPDNFTGEKQIIVEADKTSSTINIIYQDSGVGMSDDIKARAFEP
FFTTAKQSGGTGLGMSIIHNLVTQKLNGRISLISAPNQGVKIEMQLPEE
>Mature_448_residues
TRMFDRILFDVSQSPLIDKGDLTVAAQLIVSSVCKGLNVKRVGIWLLSEDRQSISCFYLSHHPQLEQTLTLARVQYPHYF
DLLDTERTIVLDHRQLDFTTQELASTYFNANNITAILASPIRHDGKMVGIICVEHQGEPREWMPDDKAFVSTLAEIFARS
ISARDILDYQRQLKVVNADLELRTQELEAALAHLSATQNHLIEKEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCLDELA
LLKRFFESNELDQEKFLNFMTTMQDGLSLVDRNLARAAELVHNFKRTAADQSIWERERFNLKAYILQVFSSLKPLMRKKS
ISLTINVDDGIYLYSYPGAIAQIFTNLVSNSFRHGFPDNFTGEKQIIVEADKTSSTINIIYQDSGVGMSDDIKARAFEPF
FTTAKQSGGTGLGMSIIHNLVTQKLNGRISLISAPNQGVKIEMQLPEE

Specific function: Phosphorylates the sporulation-regulatory proteins spo0A and spo0F. Spo0F is required for the kinB activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=270, Percent_Identity=28.1481481481481, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788393, Length=134, Percent_Identity=33.5820895522388, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693157, Length=241, Percent_Identity=25.7261410788382, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=274, Percent_Identity=26.2773722627737, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081816, Length=286, Percent_Identity=25.8741258741259, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 50412; Mature: 50281

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMFDRILFDVSQSPLIDKGDLTVAAQLIVSSVCKGLNVKRVGIWLLSEDRQSISCFYL
CCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEEEE
SHHPQLEQTLTLARVQYPHYFDLLDTERTIVLDHRQLDFTTQELASTYFNANNITAILAS
ECCCCHHHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEC
PIRHDGKMVGIICVEHQGEPREWMPDDKAFVSTLAEIFARSISARDILDYQRQLKVVNAD
HHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCC
LELRTQELEAALAHLSATQNHLIEKEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCLDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALLKRFFESNELDQEKFLNFMTTMQDGLSLVDRNLARAAELVHNFKRTAADQSIWERERF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLKAYILQVFSSLKPLMRKKSISLTINVDDGIYLYSYPGAIAQIFTNLVSNSFRHGFPDN
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
FTGEKQIIVEADKTSSTINIIYQDSGVGMSDDIKARAFEPFFTTAKQSGGTGLGMSIIHN
CCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCCHHHHHHHH
LVTQKLNGRISLISAPNQGVKIEMQLPEE
HHHHHHCCCEEEEECCCCCEEEEEECCCC
>Mature Secondary Structure 
TRMFDRILFDVSQSPLIDKGDLTVAAQLIVSSVCKGLNVKRVGIWLLSEDRQSISCFYL
CHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEEEEE
SHHPQLEQTLTLARVQYPHYFDLLDTERTIVLDHRQLDFTTQELASTYFNANNITAILAS
ECCCCHHHHHHHHHHCCCCEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEEC
PIRHDGKMVGIICVEHQGEPREWMPDDKAFVSTLAEIFARSISARDILDYQRQLKVVNAD
HHCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEECCC
LELRTQELEAALAHLSATQNHLIEKEKMAALGSLVAGVAHEVNTPLGIAVTSVTHCLDEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALLKRFFESNELDQEKFLNFMTTMQDGLSLVDRNLARAAELVHNFKRTAADQSIWERERF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLKAYILQVFSSLKPLMRKKSISLTINVDDGIYLYSYPGAIAQIFTNLVSNSFRHGFPDN
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHCCCCCCC
FTGEKQIIVEADKTSSTINIIYQDSGVGMSDDIKARAFEPFFTTAKQSGGTGLGMSIIHN
CCCCCEEEEEECCCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHCCCCCCHHHHHHHH
LVTQKLNGRISLISAPNQGVKIEMQLPEE
HHHHHHCCCEEEEECCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]