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The map label for this gene is rffG [H]

Identifier: 120598295

GI number: 120598295

Start: 1663835

End: 1664164

Strand: Direct

Name: rffG [H]

Synonym: Sputw3181_1473

Alternate gene names: 120598295

Gene position: 1663835-1664164 (Clockwise)

Preceding gene: 120598293

Following gene: 120598296

Centisome position: 35.34

GC content: 42.73

Gene sequence:

>330_bases
TTGTTTTTAGTCGCAACTCAGGGGCGACTAGGGCAGACCTATAATATTGGTGGTTCGAATGAGCGCAGTAATATTGAGGT
AGTACAGAAAATTTGTGATTTATTGGAGGAGTTAGCGCCTACTCATCCCCATGCATTTGCTGTAAATGGGATTGGTTTTC
GAGGTTTAATACAGCATGTTGAAGATCGTCCCGGCTATGATGTTCGTTATGCAATTGATGCCAGTAAACTTGAGCATGAA
CTTGGGTGGCAACCGTATGAGTCTTTTGAGTCTGGCCTAAGAAAAACCGTTAAATGGATAGTTGAACAGAGCGATGAAGT
ACGCTCGTAA

Upstream 100 bases:

>100_bases
TAGTTTTTGGAGAAACCATTTGATCATGCAACAAGATGCGCTTCCACCCTAATTTGATGATTTATTTGTAAATCATCAGG
GGATTCTTCAGGTTCGAGCG

Downstream 100 bases:

>100_bases
GCTGGCGGCTAAATGGCTTTGTTGAAAGCTAAGAGAAGCATTCTCAAATTTTATAGCTAGGGCGTCACTTTGCTTTTTTA
GAGCGCTTCGCTTGCCCTGG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 109

Protein sequence:

>109_residues
MFLVATQGRLGQTYNIGGSNERSNIEVVQKICDLLEELAPTHPHAFAVNGIGFRGLIQHVEDRPGYDVRYAIDASKLEHE
LGWQPYESFESGLRKTVKWIVEQSDEVRS

Sequences:

>Translated_109_residues
MFLVATQGRLGQTYNIGGSNERSNIEVVQKICDLLEELAPTHPHAFAVNGIGFRGLIQHVEDRPGYDVRYAIDASKLEHE
LGWQPYESFESGLRKTVKWIVEQSDEVRS
>Mature_109_residues
MFLVATQGRLGQTYNIGGSNERSNIEVVQKICDLLEELAPTHPHAFAVNGIGFRGLIQHVEDRPGYDVRYAIDASKLEHE
LGWQPYESFESGLRKTVKWIVEQSDEVRS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994969, Length=101, Percent_Identity=60.3960396039604, Blast_Score=133, Evalue=2e-33,
Organism=Escherichia coli, GI1788353, Length=112, Percent_Identity=49.1071428571429, Blast_Score=114, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568069, Length=101, Percent_Identity=37.6237623762376, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 12344; Mature: 12344

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLVATQGRLGQTYNIGGSNERSNIEVVQKICDLLEELAPTHPHAFAVNGIGFRGLIQHV
CEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
EDRPGYDVRYAIDASKLEHELGWQPYESFESGLRKTVKWIVEQSDEVRS
HCCCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHCCCC
>Mature Secondary Structure
MFLVATQGRLGQTYNIGGSNERSNIEVVQKICDLLEELAPTHPHAFAVNGIGFRGLIQHV
CEEEECCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHH
EDRPGYDVRYAIDASKLEHELGWQPYESFESGLRKTVKWIVEQSDEVRS
HCCCCCCEEEEECHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]