Click here to switch to the map view.

The map label for this gene is rfbD [C]

Identifier: 120598287

GI number: 120598287

Start: 1656059

End: 1656985

Strand: Direct

Name: rfbD [C]

Synonym: Sputw3181_1465

Alternate gene names: 120598287

Gene position: 1656059-1656985 (Clockwise)

Preceding gene: 120598286

Following gene: 120598288

Centisome position: 35.17

GC content: 43.91

Gene sequence:

>927_bases
ATGTTGAACCCTGCATACGTGGGGATGATGGACCTAATCCCGAGAACAATTATGAAAATCCTTATTACCGGAAGTAATGG
TCAGGTAGGTAGCAGCCTTGTAAAACAATTAAACCAGATGCCAGAGATTGAATTTTTGGCAGTGGATCGTCAACAATTGG
ATATTACTGACTGCGAAGCGGTAAACAAACTTGTTAATGAATTGAAACCAAACGCAATTATTAATGCGGCGGCGCATACT
GCTGTAGATAGAGCTGAGCAAGAAGTTGAGCTTTCCTACGCCATTAATCGTGACGGACCACAGTTTTTAGCACAAGCGGC
TAACAGTGTGGGTGCAACAATACTGCATATCTCAACTGACTATGTGTTTGCTGGTGATAAAGACGGTGAGTATATTGAAA
CAGATACCGTTGCTCCGCAAGGTGTATATGGTCAAAGTAAACTGGCAGGCGAACTCGCCGTTACAGCGGCTTGCCCGCGC
CATATCATCTTGCGTACCGCCTGGGTGTTTTATGGGACAGGCAATAATTTTGTTAAAACCATGCTGCGCCTTGCGCATAA
TAGAGATGAACTCGGTGTGGTGGCGGATCAATTTGGTGGCCCAACCTATGCTGGAGATATTGCCAATGCGCTTATCGTAA
TGGCTAAAGCACTGCAAGCAGGCAACACCCACTACGGAGTTTATCATTTTGCAGGTAAGCCGCATGTCAGCTGGCACCAA
TTCGCCAGAGAAATTTTCAAAAAGGCAAATGAGCAAAATATATTAAATAAACAGATGAAGGTGAACGCTATCACTTCAGA
GCAATATCTTACACCCGCAAAACGCCCTGCTAATTCCAAATTGGATTGCCTCAAAATTGAGCAAAGCTTTGGTATTAAGC
CCAGTAATTGGCAAGCGGCTTTGAATAATTTAAGTGGTTATGTGTGA

Upstream 100 bases:

>100_bases
AGCGCAATAATTAACGTTATTTCGTAGCCAGCGCACAACTTACACCCTTTATTTGTCATTCCCGCGAAAGCGGAAATCCA
TTCTTTTACATTGTGCTTAT

Downstream 100 bases:

>100_bases
GTGGCTTGTTGGGAATGATTACGCGATAACTATTATCTCACCATTCACCATTCACCATTCACCATTCATTATTAACAACA
CACGAATTAAAACATGAAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MLNPAYVGMMDLIPRTIMKILITGSNGQVGSSLVKQLNQMPEIEFLAVDRQQLDITDCEAVNKLVNELKPNAIINAAAHT
AVDRAEQEVELSYAINRDGPQFLAQAANSVGATILHISTDYVFAGDKDGEYIETDTVAPQGVYGQSKLAGELAVTAACPR
HIILRTAWVFYGTGNNFVKTMLRLAHNRDELGVVADQFGGPTYAGDIANALIVMAKALQAGNTHYGVYHFAGKPHVSWHQ
FAREIFKKANEQNILNKQMKVNAITSEQYLTPAKRPANSKLDCLKIEQSFGIKPSNWQAALNNLSGYV

Sequences:

>Translated_308_residues
MLNPAYVGMMDLIPRTIMKILITGSNGQVGSSLVKQLNQMPEIEFLAVDRQQLDITDCEAVNKLVNELKPNAIINAAAHT
AVDRAEQEVELSYAINRDGPQFLAQAANSVGATILHISTDYVFAGDKDGEYIETDTVAPQGVYGQSKLAGELAVTAACPR
HIILRTAWVFYGTGNNFVKTMLRLAHNRDELGVVADQFGGPTYAGDIANALIVMAKALQAGNTHYGVYHFAGKPHVSWHQ
FAREIFKKANEQNILNKQMKVNAITSEQYLTPAKRPANSKLDCLKIEQSFGIKPSNWQAALNNLSGYV
>Mature_308_residues
MLNPAYVGMMDLIPRTIMKILITGSNGQVGSSLVKQLNQMPEIEFLAVDRQQLDITDCEAVNKLVNELKPNAIINAAAHT
AVDRAEQEVELSYAINRDGPQFLAQAANSVGATILHISTDYVFAGDKDGEYIETDTVAPQGVYGQSKLAGELAVTAACPR
HIILRTAWVFYGTGNNFVKTMLRLAHNRDELGVVADQFGGPTYAGDIANALIVMAKALQAGNTHYGVYHFAGKPHVSWHQ
FAREIFKKANEQNILNKQMKVNAITSEQYLTPAKRPANSKLDCLKIEQSFGIKPSNWQAALNNLSGYV

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=306, Percent_Identity=26.4705882352941, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI11034825, Length=306, Percent_Identity=26.4705882352941, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1788352, Length=291, Percent_Identity=41.2371134020619, Blast_Score=228, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 33705; Mature: 33705

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNPAYVGMMDLIPRTIMKILITGSNGQVGSSLVKQLNQMPEIEFLAVDRQQLDITDCEA
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
VNKLVNELKPNAIINAAAHTAVDRAEQEVELSYAINRDGPQFLAQAANSVGATILHISTD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCEEEEEEEEC
YVFAGDKDGEYIETDTVAPQGVYGQSKLAGELAVTAACPRHIILRTAWVFYGTGNNFVKT
EEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEEEEEEECCCHHHHH
MLRLAHNRDELGVVADQFGGPTYAGDIANALIVMAKALQAGNTHYGVYHFAGKPHVSWHQ
HHHHHCCCCHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
FAREIFKKANEQNILNKQMKVNAITSEQYLTPAKRPANSKLDCLKIEQSFGIKPSNWQAA
HHHHHHHHCCHHHHHHHHHEEEEECCHHHCCCCCCCCCCCCCEEEEHHHCCCCCCHHHHH
LNNLSGYV
HHHCCCCC
>Mature Secondary Structure
MLNPAYVGMMDLIPRTIMKILITGSNGQVGSSLVKQLNQMPEIEFLAVDRQQLDITDCEA
CCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHH
VNKLVNELKPNAIINAAAHTAVDRAEQEVELSYAINRDGPQFLAQAANSVGATILHISTD
HHHHHHHHCCCCEEHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHCCEEEEEEEEC
YVFAGDKDGEYIETDTVAPQGVYGQSKLAGELAVTAACPRHIILRTAWVFYGTGNNFVKT
EEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHEEEEEEEEECCCHHHHH
MLRLAHNRDELGVVADQFGGPTYAGDIANALIVMAKALQAGNTHYGVYHFAGKPHVSWHQ
HHHHHCCCCHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
FAREIFKKANEQNILNKQMKVNAITSEQYLTPAKRPANSKLDCLKIEQSFGIKPSNWQAA
HHHHHHHHCCHHHHHHHHHEEEEECCHHHCCCCCCCCCCCCCEEEEHHHCCCCCCHHHHH
LNNLSGYV
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]