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The map label for this gene is mlaA [H]

Identifier: 120598279

GI number: 120598279

Start: 1642680

End: 1643462

Strand: Direct

Name: mlaA [H]

Synonym: Sputw3181_1456

Alternate gene names: 120598279

Gene position: 1642680-1643462 (Clockwise)

Preceding gene: 120598276

Following gene: 120598280

Centisome position: 34.89

GC content: 44.06

Gene sequence:

>783_bases
ATGAAGTTGAAATGGATAGGGCTATCGTTAGGATTTGTATTATTGCCCCAAGCATATGGGGCAGAAGTCTCCGTACCTGA
TACCACTTCTCAAGACATTACAGCAGTGACCATTACCTATGATGATCCACGGGATCCTTTCGAGGGTTTTAACCGTGCAA
TGTGGGATTTTAACTATCTGTATCTTGATAAATATCTCTATCGTCCAGTTGCCCATGGTTATAACGACTATATTCCCCGG
CCCGCTAAAACGGGTATTAATAATTTTGTACAGAACTTAGAAGAGCCAAGTAGCTTAGTTAACAATGTGTTGCAAGGCAA
GTGGGGATGGGCTGCGAATGCTGGCGGGCGTTTTACCGTTAACTCCACAATAGGTTTGTTAGGCGTTTTTGATGTGGCCG
ATATGATGGGAATGCCGCGTAAGCAAGACGCATTTAACGAAGTGCTGGGTTACTATGGTGTGCCAAATGGTCCTTATTTT
ATGGCGCCATTTGCGGGCCCCTATGTTGTGCGTGAACTCGCAACAGATTGGGTTGATGGTCTATACTTTCCACTATCAGA
GCTCACCATGTGGCAATCCGTTGTTAGGTGGGGGCTTAAGAGTCTTCATGCGAGAGCTTCAGCGATTGATCAAGAAAGGC
TCGTTGATAATGCGCTTGATCCCTATGCTTTCGTGAAAGATGCGTATTTACAACACATGGACTATAAAGTCTATGATGGC
AATGTTCCTCAGAAACAAGAAGATGATGAGTTGCTTGATCAATATATGCAGGAGCTTGATTAA

Upstream 100 bases:

>100_bases
ATATACGGCTAAGAGGAATGTTACTTATATCGTCACTTGTCTATAGTCACTTTAGCGAAAACTTTGCACCTAAGCAGTAC
ATTTATATTAAGAGATCAAT

Downstream 100 bases:

>100_bases
CGTTACGCAACATGGCTATCGATGTTGCTTTCACAACCTAGTCCTTGCCGTATCGAATGATTCTTCCGATGTCATGATTG
AGTTATTGCTATAGCAATGA

Product: VacJ family lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKLKWIGLSLGFVLLPQAYGAEVSVPDTTSQDITAVTITYDDPRDPFEGFNRAMWDFNYLYLDKYLYRPVAHGYNDYIPR
PAKTGINNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTVNSTIGLLGVFDVADMMGMPRKQDAFNEVLGYYGVPNGPYF
MAPFAGPYVVRELATDWVDGLYFPLSELTMWQSVVRWGLKSLHARASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYDG
NVPQKQEDDELLDQYMQELD

Sequences:

>Translated_260_residues
MKLKWIGLSLGFVLLPQAYGAEVSVPDTTSQDITAVTITYDDPRDPFEGFNRAMWDFNYLYLDKYLYRPVAHGYNDYIPR
PAKTGINNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTVNSTIGLLGVFDVADMMGMPRKQDAFNEVLGYYGVPNGPYF
MAPFAGPYVVRELATDWVDGLYFPLSELTMWQSVVRWGLKSLHARASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYDG
NVPQKQEDDELLDQYMQELD
>Mature_260_residues
MKLKWIGLSLGFVLLPQAYGAEVSVPDTTSQDITAVTITYDDPRDPFEGFNRAMWDFNYLYLDKYLYRPVAHGYNDYIPR
PAKTGINNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTVNSTIGLLGVFDVADMMGMPRKQDAFNEVLGYYGVPNGPYF
MAPFAGPYVVRELATDWVDGLYFPLSELTMWQSVVRWGLKSLHARASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYDG
NVPQKQEDDELLDQYMQELD

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=265, Percent_Identity=36.9811320754717, Blast_Score=161, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29577; Mature: 29577

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKWIGLSLGFVLLPQAYGAEVSVPDTTSQDITAVTITYDDPRDPFEGFNRAMWDFNYL
CCEEEEEHHHHHHHHCHHCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
YLDKYLYRPVAHGYNDYIPRPAKTGINNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTV
HHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEE
NSTIGLLGVFDVADMMGMPRKQDAFNEVLGYYGVPNGPYFMAPFAGPYVVRELATDWVDG
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LYFPLSELTMWQSVVRWGLKSLHARASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECC
NVPQKQEDDELLDQYMQELD
CCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKLKWIGLSLGFVLLPQAYGAEVSVPDTTSQDITAVTITYDDPRDPFEGFNRAMWDFNYL
CCEEEEEHHHHHHHHCHHCCCEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
YLDKYLYRPVAHGYNDYIPRPAKTGINNFVQNLEEPSSLVNNVLQGKWGWAANAGGRFTV
HHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEEE
NSTIGLLGVFDVADMMGMPRKQDAFNEVLGYYGVPNGPYFMAPFAGPYVVRELATDWVDG
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHH
LYFPLSELTMWQSVVRWGLKSLHARASAIDQERLVDNALDPYAFVKDAYLQHMDYKVYDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECC
NVPQKQEDDELLDQYMQELD
CCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]