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The map label for this gene is flgJ

Identifier: 120598237

GI number: 120598237

Start: 1600164

End: 1601321

Strand: Direct

Name: flgJ

Synonym: Sputw3181_1414

Alternate gene names: 120598237

Gene position: 1600164-1601321 (Clockwise)

Preceding gene: 120598236

Following gene: 120598238

Centisome position: 33.99

GC content: 44.65

Gene sequence:

>1158_bases
ATGGAAAAGCTTTCAAGTTCATCACATTTTCTTGACCTGGGAGGCTTAGATTCACTTCGCGCTCAGGCACAGAAAGATGA
GAAGGGCACTTTAAAACAAGTTGCCCAACAATTTGAAGGCATTTTCGTGCAAATGTTGATGAAAAGCATGCGCGATGCTA
ATGCGGTATTTGAATCGGATAGTCCCCTCAATAGCCAATACACTAAGTTCTATGAACAAATGCGTGATCAGCAATTGTCT
GTGGATTTGTCAGATAAAGGAGTATTAGGACTGGCTGACATGATGGTGCAGCAGCTCTCGCCTGAATCTAGCCAGCTTAC
ACCAGCCTCAGTATTACGTAATGATGGTGGTGAAAAACTACAGCGAGGTGATAAAGCATTCACAGCTCCTGCACAAAATA
CATCAACCCAAGATGTACTTGATGCTTCAACACCACCTGTGAGTTCTTCAACACAGATCCCAACGTATATCGCTCGTCCA
ACATTTGAGTCCGATAGACCCGAGGCTGTGACATCATCATTGGATATCGACACTCCAATACCTTCACTCGCGATAAATAC
GCCCAAACCTGCGTGGTCAGAGCAACCATTATCGCCGATTGAGACCGTCATCAGTGGCCAGATTTTGCCGACTGTGGCAT
TTAAAGAAACTCAAAAAACGCTCAAGTTTGGTAGCCGAGAAGAGTTTTTGGCGACCTTGTATCCCCATGCTGAAAAAGCG
GCTAAGGCTTTAGGTACGAAACCAGAAGTGCTACTTGCACAATCTGCACTCGAAACCGGTTGGGGACAAAAAATAGTACG
TGGCAGTAATGGTGCGCCTAGCCACAACTTATTCAATATTAAAGCTGATCGCCGTTGGCTTGGAGATAAAGCTAACGTGA
GTACCTTGGAATTTGAACAGGGTATTGCCGTGAGACAGAAAGCCGATTTTCGTGTTTACACCGACTTTGAGCATAGTTTC
AATGATTTTGTCACCTTTATTGCTGAAGGTGAGCGTTATCAAGGTGCTACAAAAGTCGCCGCTTCACCAACCCAATTTAT
TCGTGCATTGAAGGATGCGGGCTATGCCACTGATCCTAAATATGCTGAGAAAGTCATTAAAGTGATGCAGACCATAAGCC
AAGAGCTTAAGTCGATACTGCCAGGAGAAGACAAATGA

Upstream 100 bases:

>100_bases
GTGCGGCCCCTTCAGATGTACTTGCTATCCTAGAAGCGTTAAAAGTGGCAGGTGCTTTGCATGGTGAGCTTATCATCATC
TAATTTCTGTCGGGAAAACT

Downstream 100 bases:

>100_bases
TAAATCTTCATAGGATATTCGTTCTGAGGAGTGTCGGCTAATGGCGATTGACCTATTAAATATTGCACGTACTGGTGTGC
TTGCCTCTCAATCGCAGCTA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQLTPASVLRNDGGEKLQRGDKAFTAPAQNTSTQDVLDASTPPVSSSTQIPTYIARP
TFESDRPEAVTSSLDIDTPIPSLAINTPKPAWSEQPLSPIETVISGQILPTVAFKETQKTLKFGSREEFLATLYPHAEKA
AKALGTKPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDFEHSF
NDFVTFIAEGERYQGATKVAASPTQFIRALKDAGYATDPKYAEKVIKVMQTISQELKSILPGEDK

Sequences:

>Translated_385_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQLTPASVLRNDGGEKLQRGDKAFTAPAQNTSTQDVLDASTPPVSSSTQIPTYIARP
TFESDRPEAVTSSLDIDTPIPSLAINTPKPAWSEQPLSPIETVISGQILPTVAFKETQKTLKFGSREEFLATLYPHAEKA
AKALGTKPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDFEHSF
NDFVTFIAEGERYQGATKVAASPTQFIRALKDAGYATDPKYAEKVIKVMQTISQELKSILPGEDK
>Mature_385_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQLTPASVLRNDGGEKLQRGDKAFTAPAQNTSTQDVLDASTPPVSSSTQIPTYIARP
TFESDRPEAVTSSLDIDTPIPSLAINTPKPAWSEQPLSPIETVISGQILPTVAFKETQKTLKFGSREEFLATLYPHAEKA
AKALGTKPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQGIAVRQKADFRVYTDFEHSF
NDFVTFIAEGERYQGATKVAASPTQFIRALKDAGYATDPKYAEKVIKVMQTISQELKSILPGEDK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=186, Percent_Identity=39.7849462365591, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 42320; Mature: 42320

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQLTPASVLRNDGGEKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
QRGDKAFTAPAQNTSTQDVLDASTPPVSSSTQIPTYIARPTFESDRPEAVTSSLDIDTPI
HCCCCCEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCC
PSLAINTPKPAWSEQPLSPIETVISGQILPTVAFKETQKTLKFGSREEFLATLYPHAEKA
CCCEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
AKALGTKPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQ
HHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEHHC
GIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQGATKVAASPTQFIRALKDAGYATDPK
CCHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHCCCHHHCCCHHHHHHHHHHCCCCCCHH
YAEKVIKVMQTISQELKSILPGEDK
HHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGTLKQVAQQFEGIFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQLTPASVLRNDGGEKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHH
QRGDKAFTAPAQNTSTQDVLDASTPPVSSSTQIPTYIARPTFESDRPEAVTSSLDIDTPI
HCCCCCEECCCCCCCHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHCCCCCCCC
PSLAINTPKPAWSEQPLSPIETVISGQILPTVAFKETQKTLKFGSREEFLATLYPHAEKA
CCCEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHH
AKALGTKPEVLLAQSALETGWGQKIVRGSNGAPSHNLFNIKADRRWLGDKANVSTLEFEQ
HHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCEEEEECCHHHCCCCCCCCEEEHHC
GIAVRQKADFRVYTDFEHSFNDFVTFIAEGERYQGATKVAASPTQFIRALKDAGYATDPK
CCHHHCCCCEEEEECHHHHHHHHHHHHHCCCHHCCCHHHCCCHHHHHHHHHHCCCCCCHH
YAEKVIKVMQTISQELKSILPGEDK
HHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]