Click here to switch to the map view.

The map label for this gene is trmB

Identifier: 120598135

GI number: 120598135

Start: 1495375

End: 1496091

Strand: Direct

Name: trmB

Synonym: Sputw3181_1312

Alternate gene names: 120598135

Gene position: 1495375-1496091 (Clockwise)

Preceding gene: 120598132

Following gene: 120598136

Centisome position: 31.76

GC content: 46.58

Gene sequence:

>717_bases
ATGAGCGAAGTCACTACCGCTGAATTTAATGAAGAAGGCAAGTATCTGCGCAAGATCAGAAGCTTTGTCCTGAGAGAAGG
TCGCTTAACTAAAGGCCAAGCCCAAGCTATTGAAAGCCAGTGGCCAACAATGGGTTTAGATTACAGCCCAGTGCCATTAA
ACCTTAGCGAGGTTTTTGGTCGTGAAGCCGATACTGTGCTGGAAATTGGCTTTGGCATGGGTGCCTCTTTAGTACAAATG
GCAAAAGATGCACCGGAGCAAAATTTCATTGGTATTGAAGTACATAAACCGGGTGTAGGCTCTTGCTTAAGTGATGCTGC
CATTGCCGGTGTGACTAATTTACGTGTATATCATCACGATGCCATGGAAGTATTAGAACATGCGATTGCTGACGGCTCAT
TAGCTCGAGTGCAATTGTTTTTCCCCGATCCTTGGCATAAAAAACGTCACCATAAGCGCCGCATCGTACAAGCTGAATTT
GCCGAACTTGTTCGTAGCAAGCTAAAAATTGGTGGTGTGTTCCATATGGCCACTGACTGGGAAGAATATAGCGAGCATAT
GCTCGAGGTAATGAATGCGGCGCCCGGTTATAAAAACCAATCGGTCGATGACACCGTTGTGCCACGTCCTGATCATCGCC
CACTGACAAAGTTTGAAGCCCGCGGCCATAGATTGGGTCACGGTGTATGGGATCTGATGTTTGAGCGTATCGTTTAA

Upstream 100 bases:

>100_bases
AATCTTGGTATACTTTCGCCCCATTAGTGCTTGCACTGTATGTTGAACTTTGGATAATGCCCTTCTTTTATGATGGTGCT
GTCCACTAGACGAGGCAAAA

Downstream 100 bases:

>100_bases
TCACGAACTCAATTTTAGCTAATTTTAAACACAAAAGGGAAAATCACTATGGCAACTAATCGTAGCCGTCGTTTACGTAA
GAAAATGCGCGTCGATGAAT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPVPLNLSEVFGREADTVLEIGFGMGASLVQM
AKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELVRSKLKIGGVFHMATDWEEYSEHMLEVMNAAPGYKNQSVDDTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIV

Sequences:

>Translated_238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPVPLNLSEVFGREADTVLEIGFGMGASLVQM
AKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELVRSKLKIGGVFHMATDWEEYSEHMLEVMNAAPGYKNQSVDDTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIV
>Mature_237_residues
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPVPLNLSEVFGREADTVLEIGFGMGASLVQMA
KDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFA
ELVRSKLKIGGVFHMATDWEEYSEHMLEVMNAAPGYKNQSVDDTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIV

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=66.5254237288136, Blast_Score=331, Evalue=2e-92,
Organism=Saccharomyces cerevisiae, GI6320000, Length=146, Percent_Identity=28.7671232876712, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26859; Mature: 26728

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPVPLNLSEVFG
CCCCCCCHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
READTVLEIGFGMGASLVQMAKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELVRSKLKIGGVFHMATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCH
EEYSEHMLEVMNAAPGYKNQSVDDTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPVPLNLSEVFG
CCCCCCHHCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
READTVLEIGFGMGASLVQMAKDAPEQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCCHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELVRSKLKIGGVFHMATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCH
EEYSEHMLEVMNAAPGYKNQSVDDTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA