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The map label for this gene is thiL [H]

Identifier: 120598062

GI number: 120598062

Start: 1400978

End: 1401949

Strand: Direct

Name: thiL [H]

Synonym: Sputw3181_1239

Alternate gene names: 120598062

Gene position: 1400978-1401949 (Clockwise)

Preceding gene: 120598061

Following gene: 120598063

Centisome position: 29.75

GC content: 50.51

Gene sequence:

>972_bases
GTGCCAAACTGTACAGTGAAAGAATTCCAATTAATTGAATGTTATTTTAGCAACCGCGGCCCCACTCGCCGTGATGTGAA
GCTTGGCATCGGTGACGATTGCGCTTTAGTGCAACCCGCAGAAAACAAGTCGATTGCTATCTCCTGTGACACCTTAGTCG
AAAATGTCCATTTCTTCCCCGATATGCCTCCTGCAGCCTTAGGTTATAAAGCCTTAGCGGTAAATTTATCTGATCTGGCA
GCCATGGGCGCTGAACCTGCGTGGATGACGCTTGCCTTAACGCTACCCGAAGTCGATGAAACCTGGCTAAGTGGTTTTAG
TGAAGGCTTGTTTGAAGCCGCCGAGTACTATGGCATCGCCTTAATTGGTGGTGATACGACTCGTGGTCCACGGGCGATTA
ACATCACAGTGCACGGCCAAGTCCCACAGGGTAAGGCACTCACTCGTCATGGTGCTAAAGTGGGAGATTGGATCTATGTT
ACGGGCACACTCGGAGATTCGGCCTTAGGTCTGGATATCATTCGAGGCGCGCAACAGGCCAGAGCCGAGCATAAAGAATT
CCTGATTAATCGCCATTATCGTCCAACACCAAGAGTTTTAGCGGGCCAAGCTTTGCGTTCGCTTGCCTCCAGTGCTATCG
ACCTTTCTGACGGGTTTATCTCGGATATAGGCCATATTCTTAAGGCTTCTGGTGTGGGAGCCATTGTCGATGTGGCTAAT
ATTCCTCTGTCCCGTGCGATGCAAGATACCGTTAGTGAGGAGCATGCCTTAGGTTATGCGCTGACCGGTGGCGAAGATTA
TGAACTGCTGTTTACGGTTCCTGAGTCCCAAAAGGGCGCGCTTGAAACCGCCTTGAGCCATGCGGGGGCAAAGTTTGTTC
GTGTTGGACAAATTTGTGCGGGCAGTAAGCTCAAGTTGCAACGCAATGGTGAGCCGTTTACGCCGCCGTATCACGGTTTC
GAGCACTTTTAA

Upstream 100 bases:

>100_bases
GAACGGACTGCTCGACAAACTGGTTGCACGCAAGTAATAAAGACAAACGGTATCTTAGGATACCGTTTTTCATTGCTAAC
AAAGGTGGCCGATGTAAGTC

Downstream 100 bases:

>100_bases
GCGAAGATCATATTAATGAAATGGTTGTCAAAGGATCAGGTGCTAACGCGCCTATCATTAAAAAATCCTGTCCACTTTTT
GGCACTCGGTTTCGGTAGTG

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPNCTVKEFQLIECYFSNRGPTRRDVKLGIGDDCALVQPAENKSIAISCDTLVENVHFFPDMPPAALGYKALAVNLSDLA
AMGAEPAWMTLALTLPEVDETWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKVGDWIYV
TGTLGDSALGLDIIRGAQQARAEHKEFLINRHYRPTPRVLAGQALRSLASSAIDLSDGFISDIGHILKASGVGAIVDVAN
IPLSRAMQDTVSEEHALGYALTGGEDYELLFTVPESQKGALETALSHAGAKFVRVGQICAGSKLKLQRNGEPFTPPYHGF
EHF

Sequences:

>Translated_323_residues
MPNCTVKEFQLIECYFSNRGPTRRDVKLGIGDDCALVQPAENKSIAISCDTLVENVHFFPDMPPAALGYKALAVNLSDLA
AMGAEPAWMTLALTLPEVDETWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKVGDWIYV
TGTLGDSALGLDIIRGAQQARAEHKEFLINRHYRPTPRVLAGQALRSLASSAIDLSDGFISDIGHILKASGVGAIVDVAN
IPLSRAMQDTVSEEHALGYALTGGEDYELLFTVPESQKGALETALSHAGAKFVRVGQICAGSKLKLQRNGEPFTPPYHGF
EHF
>Mature_322_residues
PNCTVKEFQLIECYFSNRGPTRRDVKLGIGDDCALVQPAENKSIAISCDTLVENVHFFPDMPPAALGYKALAVNLSDLAA
MGAEPAWMTLALTLPEVDETWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKVGDWIYVT
GTLGDSALGLDIIRGAQQARAEHKEFLINRHYRPTPRVLAGQALRSLASSAIDLSDGFISDIGHILKASGVGAIVDVANI
PLSRAMQDTVSEEHALGYALTGGEDYELLFTVPESQKGALETALSHAGAKFVRVGQICAGSKLKLQRNGEPFTPPYHGFE
HF

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=318, Percent_Identity=59.4339622641509, Blast_Score=373, Evalue=1e-105,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 34677; Mature: 34546

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNCTVKEFQLIECYFSNRGPTRRDVKLGIGDDCALVQPAENKSIAISCDTLVENVHFFP
CCCCCCHHEEEEEEEECCCCCCCCEEEECCCCCCEEECCCCCCEEEEEHHHHHHHHHCCC
DMPPAALGYKALAVNLSDLAAMGAEPAWMTLALTLPEVDETWLSGFSEGLFEAAEYYGIA
CCCHHHHCCEEEEEEHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEE
LIGGDTTRGPRAINITVHGQVPQGKALTRHGAKVGDWIYVTGTLGDSALGLDIIRGAQQA
EECCCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RAEHKEFLINRHYRPTPRVLAGQALRSLASSAIDLSDGFISDIGHILKASGVGAIVDVAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECC
IPLSRAMQDTVSEEHALGYALTGGEDYELLFTVPESQKGALETALSHAGAKFVRVGQICA
CCHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCHHEEEECHHCC
GSKLKLQRNGEPFTPPYHGFEHF
CCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNCTVKEFQLIECYFSNRGPTRRDVKLGIGDDCALVQPAENKSIAISCDTLVENVHFFP
CCCCCHHEEEEEEEECCCCCCCCEEEECCCCCCEEECCCCCCEEEEEHHHHHHHHHCCC
DMPPAALGYKALAVNLSDLAAMGAEPAWMTLALTLPEVDETWLSGFSEGLFEAAEYYGIA
CCCHHHHCCEEEEEEHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEE
LIGGDTTRGPRAINITVHGQVPQGKALTRHGAKVGDWIYVTGTLGDSALGLDIIRGAQQA
EECCCCCCCCEEEEEEEECCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHH
RAEHKEFLINRHYRPTPRVLAGQALRSLASSAIDLSDGFISDIGHILKASGVGAIVDVAN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECC
IPLSRAMQDTVSEEHALGYALTGGEDYELLFTVPESQKGALETALSHAGAKFVRVGQICA
CCHHHHHHHHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHCCHHEEEECHHCC
GSKLKLQRNGEPFTPPYHGFEHF
CCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]