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The map label for this gene is glyA

Identifier: 120598055

GI number: 120598055

Start: 1394885

End: 1396138

Strand: Direct

Name: glyA

Synonym: Sputw3181_1232

Alternate gene names: 120598055

Gene position: 1394885-1396138 (Clockwise)

Preceding gene: 120598053

Following gene: 120598056

Centisome position: 29.63

GC content: 47.37

Gene sequence:

>1254_bases
ATGCTGAAAAAAGATATGAATATCGCAGATTATGATCCGGAACTGTTTAACGCAATTCAGAATGAAACTCTGCGTCAAGA
AGAGCATATTGAGCTGATTGCTTCTGAAAACTACACCAGTCCACGCGTGATGGAAGCGCAAGGTTCACAGTTAACCAATA
AGTACGCTGAAGGCTATCCAGGCAAGCGTTACTACGGTGGTTGTGAGTATGTGGACGTAGTTGAAACCTTAGCGATTGAA
CGTGCTAAACAACTGTTTGGCGCAACTTACGCTAACGTACAACCTCATTCAGGTTCACAGGCAAACAGCGCTGTTTACAT
GGCATTGTTAAAACCTGGCGATACCGTATTGGGCATGAACTTAGCCCACGGTGGTCACTTGACCCACGGTTCACCCGTTA
ACTTCTCTGGCAAACTGTACAACATCATTCCTTACGGTATCGACGAGTCTGGCAAAATTGACTACGAAGAGATGGAACGT
ATTGCCATAGAACACAAGCCTAAGATGATGATCGGCGGTTTCTCTGCCTATTCAGGTATCGTTGACTGGGCAAAAATGCG
TGAAATCGCAGATAAAATAGGTGCTTACCTATTTGTTGATATGGCGCACGTTGCCGGTCTTATTGCTGCGGGCGTTTATC
CAAACCCAGTACCACACGCGCACGTTGTGACATCTACGACCCACAAAACCTTAGCGGGTCCTCGTGGTGGCGTGATCTTA
TCTGCTGCTGACGATGAAGACTTGTACAAAAAGTTGAACTCTGCGGTATTCCCAGGCGGTCAAGGTGGCCCATTGATGCA
CGTTATCGCTGGTAAAGCGGTGGCGTTCAAAGAAGCCCTAGAGCCAGAATTTAAAGTTTACCAACAGCAAGTGGTTAACA
ACGCTAAAGCTATGGTTGAAGTGTTCCTTGAGCGCGGTTACAAAATCGTCTCTGGCGGAACCAGCAACCACTTAATGCTA
GTTGACCTGATTGGTCGTGACTTAACGGGTAAAGAAGCCGATGCCGCCTTAGGTAGTGCTAACATTACCGTGAACAAAAA
CTCAGTGCCAAATGATCCACGTTCACCCTTTGTGACATCAGGTGTGCGTATCGGTACGCCAGCCATTACGCGCCGCGGCT
TTAAAGAAGCGGAATCGAAAGAGCTGACCGGCTGGATTTGTGACATCCTTGATGATGCTAACAATCCTGCAGTGATTGAG
CGTGTTAAAGGCCAAGTATTGGCACTGTGCGCACGCTTCCCTGTTTACGGTTAA

Upstream 100 bases:

>100_bases
ATCTGCAGGAATTTCATGTTTCAAATACTGGGATTGTGGTCACGATCCGAGTAGAATACCGCGCAACCCTACCTATAAGA
ATTGGCAGCTGGAGTAAGCA

Downstream 100 bases:

>100_bases
TCTGTAAATAGTCTCCTTTTTGTGGTGCTGAGCCTAACAAGCATCAGATGGTTAACCTCAGCCCATAAATAGGATAAAAT
CCCATGGCCGCATTAAGCGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYEEMER
IAIEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIE
RVKGQVLALCARFPVYG

Sequences:

>Translated_417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYEEMER
IAIEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIE
RVKGQVLALCARFPVYG
>Mature_417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYEEMER
IAIEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIE
RVKGQVLALCARFPVYG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=45.771144278607, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=45.771144278607, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=45.771144278607, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=45.771144278607, Blast_Score=351, Evalue=8e-97,
Organism=Homo sapiens, GI261862346, Length=410, Percent_Identity=44.390243902439, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=47.1321695760599, Blast_Score=332, Evalue=6e-91,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=44.987146529563, Blast_Score=302, Evalue=4e-82,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=83.1730769230769, Blast_Score=719, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=45.1371571072319, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=45.1371571072319, Blast_Score=339, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6319739, Length=415, Percent_Identity=44.0963855421687, Blast_Score=358, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.273631840796, Blast_Score=338, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=46.75, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=46.75, Blast_Score=360, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45220; Mature: 45220

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYEEMERIAIEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYEEMERIAIEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEAESKELTGWICDILDDANNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA