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The map label for this gene is bepF [H]

Identifier: 120598043

GI number: 120598043

Start: 1377585

End: 1378823

Strand: Direct

Name: bepF [H]

Synonym: Sputw3181_1220

Alternate gene names: 120598043

Gene position: 1377585-1378823 (Clockwise)

Preceding gene: 120598041

Following gene: 120598044

Centisome position: 29.26

GC content: 49.23

Gene sequence:

>1239_bases
ATGATGAGTAACAAACCTTTACGAATAATGATGTTAACGGCGGTGGCGGCTTTCGTCCTTTCAGCTTGTGGTGAGCAAAC
AACGCAGCAGGGGCCTGCACCGACTGCACCTAAGGTGGATGTTGCTCAAGTATTGCATGAGCGAGTTACCGAATGGGATG
AGTTTACTGGCCGTTTACAGGCACCAGAAAGTGTCACCCTTATCCCACGAGTATCTGGTTACATTGAATCAGTAAATTTT
AAAGAAGGCGCCTTAGTGAAGAAAGGTGACGTACTCTTTCGCATCGATCCAAGCGTGTTTGAAGTGGAAGTGGCCCGGTT
AAAAGCGGATCTTGCTAGCGCTATTTCTGCTGAGCAACTGGCCACTAATGATTTGGAGCGTGCCCGTAAGTTATTTGATC
AAAAGGCAGTTTCTGCAGAATTACTCGATACCCGTGAGTCAAACAAGCGCCAAACTGCGGCGGCGGTTGCGTCGGTGAAA
GCAGCACTCATGCGCGCCGAGCTTGATTTAGCTTATACCCAAGTGCAAGCGCCGATTGATGGCCGCGTGTCCTACGCCAA
CGTCACAACGGGTAACTATGTCACTGCAGGGCAGAGTGTGCTAACCAGCTTGGTTTCTACGGCCAGCATGTATGCCTATT
TCGATGTGGATGAACAAACCTACCTTAAATACGTAAAGCTAACAGCCGAGAAGAAACGTAACGATCCTCGTGCTGGTGAT
AACCCCGTGTATATGGCTTTAGCCAATGAGCGCGATTATCACCATATTGGCATGGTCGACTTTGTTGACAATGCGATGGA
TAAGCAGACGGGCACAATTCGAGTTAGAGCCACTTTTGACAATGAAGACAATAGATTATTGCCTGGCTTGTTTGCGCGTC
TTCGCACCGCGGGCAGCGGCGCCTATGAGGGGATCTTGATCGACGATAAAGCCGTTGGCACAGATCTCAATAATAAGTTT
GTGCTGGTCGTCGGTGATGATGGCACGGTCGAATATCGTGGCGTGACCTTAGGTGAGAAAGTTCAAGGGCTTCGTATCGT
AACTCAAGGTTTAGCTGCTGAAGACAAGATCGTCGTCAACGGTATGCAGCGTGTTCGCCCTAAGATGCAAATTGAGCCAA
ATATGGTGGATATGGTCGACAGCGAGAAACTCGAAGCATTGCGCCAAGCCCAGTTAATGCTCGATAAAAACCAAGATCAT
TTGACGGCGCAAGCGGTTGAAACGGCGAGCCGCGGTTAA

Upstream 100 bases:

>100_bases
TATTTTTTACCAGTCGGTATTTAATCGGTTTGGTTTGTAACACAGCGGCATGTGTGTGGTTCGAACAGCGTGAAATGCCC
GCCACCTTGAGGATAGTAAT

Downstream 100 bases:

>100_bases
GGAATAACACACTATGTTGTCGCAATTTTTCATTAAAAGACCGATATTTGCAGCCGTGCTGTCATTGCTGTTTCTTATCA
CTGGGGCGATTGCCGTTTGG

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MMSNKPLRIMMLTAVAAFVLSACGEQTTQQGPAPTAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLIPRVSGYIESVNF
KEGALVKKGDVLFRIDPSVFEVEVARLKADLASAISAEQLATNDLERARKLFDQKAVSAELLDTRESNKRQTAAAVASVK
AALMRAELDLAYTQVQAPIDGRVSYANVTTGNYVTAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRNDPRAGD
NPVYMALANERDYHHIGMVDFVDNAMDKQTGTIRVRATFDNEDNRLLPGLFARLRTAGSGAYEGILIDDKAVGTDLNNKF
VLVVGDDGTVEYRGVTLGEKVQGLRIVTQGLAAEDKIVVNGMQRVRPKMQIEPNMVDMVDSEKLEALRQAQLMLDKNQDH
LTAQAVETASRG

Sequences:

>Translated_412_residues
MMSNKPLRIMMLTAVAAFVLSACGEQTTQQGPAPTAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLIPRVSGYIESVNF
KEGALVKKGDVLFRIDPSVFEVEVARLKADLASAISAEQLATNDLERARKLFDQKAVSAELLDTRESNKRQTAAAVASVK
AALMRAELDLAYTQVQAPIDGRVSYANVTTGNYVTAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRNDPRAGD
NPVYMALANERDYHHIGMVDFVDNAMDKQTGTIRVRATFDNEDNRLLPGLFARLRTAGSGAYEGILIDDKAVGTDLNNKF
VLVVGDDGTVEYRGVTLGEKVQGLRIVTQGLAAEDKIVVNGMQRVRPKMQIEPNMVDMVDSEKLEALRQAQLMLDKNQDH
LTAQAVETASRG
>Mature_412_residues
MMSNKPLRIMMLTAVAAFVLSACGEQTTQQGPAPTAPKVDVAQVLHERVTEWDEFTGRLQAPESVTLIPRVSGYIESVNF
KEGALVKKGDVLFRIDPSVFEVEVARLKADLASAISAEQLATNDLERARKLFDQKAVSAELLDTRESNKRQTAAAVASVK
AALMRAELDLAYTQVQAPIDGRVSYANVTTGNYVTAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRNDPRAGD
NPVYMALANERDYHHIGMVDFVDNAMDKQTGTIRVRATFDNEDNRLLPGLFARLRTAGSGAYEGILIDDKAVGTDLNNKF
VLVVGDDGTVEYRGVTLGEKVQGLRIVTQGLAAEDKIVVNGMQRVRPKMQIEPNMVDMVDSEKLEALRQAQLMLDKNQDH
LTAQAVETASRG

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=375, Percent_Identity=33.0666666666667, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1789929, Length=370, Percent_Identity=28.3783783783784, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789665, Length=378, Percent_Identity=30.4232804232804, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI87082036, Length=356, Percent_Identity=28.0898876404494, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081951, Length=172, Percent_Identity=36.046511627907, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789637, Length=164, Percent_Identity=34.7560975609756, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790519, Length=216, Percent_Identity=26.8518518518519, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45211; Mature: 45211

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSNKPLRIMMLTAVAAFVLSACGEQTTQQGPAPTAPKVDVAQVLHERVTEWDEFTGRLQ
CCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
APESVTLIPRVSGYIESVNFKEGALVKKGDVLFRIDPSVFEVEVARLKADLASAISAEQL
CCCCEEEEECHHHHHHCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ATNDLERARKLFDQKAVSAELLDTRESNKRQTAAAVASVKAALMRAELDLAYTQVQAPID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GRVSYANVTTGNYVTAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRNDPRAGD
CCEEEEEECCCCEEECCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC
NPVYMALANERDYHHIGMVDFVDNAMDKQTGTIRVRATFDNEDNRLLPGLFARLRTAGSG
CCEEEEEECCCCCEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
AYEGILIDDKAVGTDLNNKFVLVVGDDGTVEYRGVTLGEKVQGLRIVTQGLAAEDKIVVN
CCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHCCHHHHHHCCCCCCHHEEC
GMQRVRPKMQIEPNMVDMVDSEKLEALRQAQLMLDKNQDHLTAQAVETASRG
CHHHCCCCCEECCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MMSNKPLRIMMLTAVAAFVLSACGEQTTQQGPAPTAPKVDVAQVLHERVTEWDEFTGRLQ
CCCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
APESVTLIPRVSGYIESVNFKEGALVKKGDVLFRIDPSVFEVEVARLKADLASAISAEQL
CCCCEEEEECHHHHHHCCCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ATNDLERARKLFDQKAVSAELLDTRESNKRQTAAAVASVKAALMRAELDLAYTQVQAPID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
GRVSYANVTTGNYVTAGQSVLTSLVSTASMYAYFDVDEQTYLKYVKLTAEKKRNDPRAGD
CCEEEEEECCCCEEECCHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCCCCC
NPVYMALANERDYHHIGMVDFVDNAMDKQTGTIRVRATFDNEDNRLLPGLFARLRTAGSG
CCEEEEEECCCCCEEECHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCC
AYEGILIDDKAVGTDLNNKFVLVVGDDGTVEYRGVTLGEKVQGLRIVTQGLAAEDKIVVN
CCCEEEEECCCCCCCCCCEEEEEECCCCCEEEECCCCCCHHCCHHHHHHCCCCCCHHEEC
GMQRVRPKMQIEPNMVDMVDSEKLEALRQAQLMLDKNQDHLTAQAVETASRG
CHHHCCCCCEECCCHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]