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The map label for this gene is cynR [H]

Identifier: 120598039

GI number: 120598039

Start: 1374105

End: 1374995

Strand: Direct

Name: cynR [H]

Synonym: Sputw3181_1216

Alternate gene names: 120598039

Gene position: 1374105-1374995 (Clockwise)

Preceding gene: 120598037

Following gene: 120598041

Centisome position: 29.18

GC content: 44.44

Gene sequence:

>891_bases
ATGCTCGATCTTCATTGGTTAAAGACTTTTGTCACTTTGGCTGAATGTAAGCATTTTGGTAAGGCAGCCGCTGTGCTACA
TATGACTCAGCCCAATGTTAGCTTGCATATTAAGCAGTTAGAGCAAACCACTCGGGTAAAGTTGATTGACCGTAATCCCT
TTCGATTGACACAGGCGGGATTTCGTTTGTTAGAAAGCAGCCAAAAAACTCTGATGGAATTACAAATTTGCCAGGCGGAT
TTGAATGCAATTAACGATCTTAGCCAAGGCACTCTCACCATAGCAGCGAGCGATATTATCTCGCGGTTACTCTTGATCCA
ACCGTTTCAGCTCTTTAAGGCGGAGTTCCCAGGAATTGACTTTTCACTGCTTAATACCACGTCTTCACAGGCCTCTGAGT
TAGTGACCAATGCCGAAGCCGATTTAGGCTTTGTGATCGCGCAAAAGGAGAGTCAACCACTGCATTTTACTGAATTACAA
CAGATTAAATGGTGTGCCATCGGTGACCATTTGCAGCAATGGCAGCAAACAAAACTGTCCGTGGATACACCCTTAGCCGA
GCAACCGACCTTGATTTTATTAGGCCATGATACGCGAACCCGCGAGTTATTGGACCCTGCGCTACCTTCCTTGAACTTAC
CCAATTATAGGATTATGGAAGTTGGCAGTGTTGACGCACAAATCGATTGGGCAGAGGCGGGATTTGGGGTAGCGATAGTG
CCAGAGTTTTCTGTATATACGAAAGCGAATTTGACCACGAAAGTCACTCCTTTGCCGCAATTCCCTACAACGAGTTTAGG
TTATATTGTGCGTCAAAATCAGATTTTGTCTAAAGCTATCAAGCAGTTGTTATATTGGGTGAATCAAGAAATTATTCGCT
CGCAGCGATAG

Upstream 100 bases:

>100_bases
TCAGTAGGAATAAGAGAAATTAATTATAGTTATCGTGCTGATAAGAATTTCATATATAATTGGCGATAATTAACTCTATT
TCTGTCGGGATGGTGGTGCA

Downstream 100 bases:

>100_bases
GACTCAATGAGAATAGTTTTTCATACTGGCCAGTGGCTTTGAGTTGGGATAAACCAGCATCAAGTTGGCTTGCTAGGGTA
TGTGCCTGTGGATTTTCCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Cyn operon transcriptional activator [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLDLHWLKTFVTLAECKHFGKAAAVLHMTQPNVSLHIKQLEQTTRVKLIDRNPFRLTQAGFRLLESSQKTLMELQICQAD
LNAINDLSQGTLTIAASDIISRLLLIQPFQLFKAEFPGIDFSLLNTTSSQASELVTNAEADLGFVIAQKESQPLHFTELQ
QIKWCAIGDHLQQWQQTKLSVDTPLAEQPTLILLGHDTRTRELLDPALPSLNLPNYRIMEVGSVDAQIDWAEAGFGVAIV
PEFSVYTKANLTTKVTPLPQFPTTSLGYIVRQNQILSKAIKQLLYWVNQEIIRSQR

Sequences:

>Translated_296_residues
MLDLHWLKTFVTLAECKHFGKAAAVLHMTQPNVSLHIKQLEQTTRVKLIDRNPFRLTQAGFRLLESSQKTLMELQICQAD
LNAINDLSQGTLTIAASDIISRLLLIQPFQLFKAEFPGIDFSLLNTTSSQASELVTNAEADLGFVIAQKESQPLHFTELQ
QIKWCAIGDHLQQWQQTKLSVDTPLAEQPTLILLGHDTRTRELLDPALPSLNLPNYRIMEVGSVDAQIDWAEAGFGVAIV
PEFSVYTKANLTTKVTPLPQFPTTSLGYIVRQNQILSKAIKQLLYWVNQEIIRSQR
>Mature_296_residues
MLDLHWLKTFVTLAECKHFGKAAAVLHMTQPNVSLHIKQLEQTTRVKLIDRNPFRLTQAGFRLLESSQKTLMELQICQAD
LNAINDLSQGTLTIAASDIISRLLLIQPFQLFKAEFPGIDFSLLNTTSSQASELVTNAEADLGFVIAQKESQPLHFTELQ
QIKWCAIGDHLQQWQQTKLSVDTPLAEQPTLILLGHDTRTRELLDPALPSLNLPNYRIMEVGSVDAQIDWAEAGFGVAIV
PEFSVYTKANLTTKVTPLPQFPTTSLGYIVRQNQILSKAIKQLLYWVNQEIIRSQR

Specific function: Positively regulates the cynTSX operon, and negatively regulates its own transcription. Binds specifically to the cynR- cynTSX intergenic region [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=235, Percent_Identity=28.0851063829787, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788748, Length=307, Percent_Identity=26.7100977198697, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=23.5494880546075, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1790208, Length=246, Percent_Identity=27.6422764227642, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=24.7933884297521, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33315; Mature: 33315

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLHWLKTFVTLAECKHFGKAAAVLHMTQPNVSLHIKQLEQTTRVKLIDRNPFRLTQAG
CCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHCCEEEEEECCCCHHHHHH
FRLLESSQKTLMELQICQADLNAINDLSQGTLTIAASDIISRLLLIQPFQLFKAEFPGID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHCCCCC
FSLLNTTSSQASELVTNAEADLGFVIAQKESQPLHFTELQQIKWCAIGDHLQQWQQTKLS
EEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VDTPLAEQPTLILLGHDTRTRELLDPALPSLNLPNYRIMEVGSVDAQIDWAEAGFGVAIV
CCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEE
PEFSVYTKANLTTKVTPLPQFPTTSLGYIVRQNQILSKAIKQLLYWVNQEIIRSQR
CCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDLHWLKTFVTLAECKHFGKAAAVLHMTQPNVSLHIKQLEQTTRVKLIDRNPFRLTQAG
CCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEHHHHHHCCEEEEEECCCCHHHHHH
FRLLESSQKTLMELQICQADLNAINDLSQGTLTIAASDIISRLLLIQPFQLFKAEFPGID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCHHHHHHHCCCCC
FSLLNTTSSQASELVTNAEADLGFVIAQKESQPLHFTELQQIKWCAIGDHLQQWQQTKLS
EEEECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
VDTPLAEQPTLILLGHDTRTRELLDPALPSLNLPNYRIMEVGSVDAQIDWAEAGFGVAIV
CCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCCCEEEEEECCCCCEEEHHCCCCCEEEE
PEFSVYTKANLTTKVTPLPQFPTTSLGYIVRQNQILSKAIKQLLYWVNQEIIRSQR
CCCEEEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]