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The map label for this gene is oprM [H]

Identifier: 120597958

GI number: 120597958

Start: 1283212

End: 1284705

Strand: Direct

Name: oprM [H]

Synonym: Sputw3181_1131

Alternate gene names: 120597958

Gene position: 1283212-1284705 (Clockwise)

Preceding gene: 120597957

Following gene: 120597959

Centisome position: 27.25

GC content: 59.77

Gene sequence:

>1494_bases
ATGAAGAAACCTTTCGCACTCACACAACCTGCCAAGAGCTTGGCACCGGTAGCTTTCGCGATAGCGTTAGCCGGTTGTTC
CATGGCACCCAAGTACGATCGGCCGCCAGCGCCGATTGATATAGTATATCCTTCGGGCGCGGCCTATGCAGAACCGGCGA
AAGCGACGCCAGAGGCCCCAGTCACGGACGCGGCCGATATCGGCTGGCGAGATTTCTTCCGCGACCCGCTGTTGCAACAA
TTGATCGGTATCGCGCTGGAGAGCAACCGCGACATGCGCAAAGCTGCGCTCAACGTTGAGGCGGCGCAGGCACTGTACCG
CATTCAGCGCGCTGAGGTGCTCCCGAACTTGGGGGTATCCGGCAGAGGGGCCGCAGAACGACTGCCAGCCGATCTCAGCA
ACACCGGCGCTGCCGACGTCGTGCGTCGTTACGAGGTTGCTGGGGTGACCGCTGCCTGGGAACTGGACGTCTGGGGCCGT
ATCCGCAGCCTCAGCAACCAAGCGCTGGCTTCCTACTTGGCTATGGACGAGACTCGCATAGCGACGCAGATGAGTTTGAT
CTCTGAGGTTGCTAACGCCTATCTCACGTTGCGGGCTGACCAGGAGTTACTGCAGTTAACAGGCGAGACGCTCGGCACGC
AAAAGCGTTCGCATGAACTTACTGCCCAACTGTTCGAGGCTGGCAATGCCACGCAACTTGACGTGCGCCGCTCGGAAATT
GTACTGCGTACAGCCGAGGCCAACTTCGCGGACTACACACGTCAAACGGCCAAAGACCGAAACGCGCTAGTGCTACTGCT
CGGCCAGCCGCTGACGCTTGAGCTGTCGCAGCAACTTGACAAAGCCATAGTGCTGCCGGATGGCATCGTGCCGACCGAAC
TGCCCAGTGGCCTACCTTCTGAGTTGCTCGTGCGCCGACCGGATGTGCGCGCGGCCGAGCAGGTACTTCTGGGTGCCAAC
GCCAGTATCGGAGCAGCGCGAGCCGCTTTCTTCCCCACCATCAGCTTGACGGGTTCGGCGGGGACGGCAAGCGCATCGCT
TGATGGGCTCTTCGAATCGGGTTCGCGCGCCTGGAGCTTCTTGCCGCAGATTACGGTGCCTATTTTCCGGGGTGGCGCGT
TGCGCGCCAATCTGGATGTGGCGCACGTAAAAAAGCGCATCGAGATCGCCAACTACGAGAAGTCCATTCAAGTTGCTTTT
AGTGAAGTGGCTGATGGCCTTGCCGGCAAGCGAACACTTGATGAGCAGATTCGTTCAGAGCAATTCCTGGTTGCTGCTAG
TCAGGGCATGTATGACCTGGCCGAGCAACGTTTCAGGGAAGGTGTGGACGATAACCTCACCTTGCTTGACGCGCAGCGTA
CGCTGTACAGCGCGCAGCAGACGTTGGTGCGTACGCGTCTGGCTCGGTTGAGCAACCTTATCGCGCTCTACAAGGCTCTG
GGAGGCGGGTGGACAGAGAGCACACCAAGTGGTGTGGCGCAAGCGAGTCGATAG

Upstream 100 bases:

>100_bases
GTGTTCTACGTCGTAGTGCGCAAGCTGGCGTTGCGTCGTGAAGCCAGTCAAGCCCGCGTCGGTATGAGCGACAAGCATAA
CGGCCAGGAGGAATTTTGAG

Downstream 100 bases:

>100_bases
TTAGTTTTTCCCTACTGGCACTGGTATTGGATATTCGTACCAAGAAATGACGCATGGTGGCAGTCGCCTCCATGCGTGTG
TAGATGAGTATGAAAACCGG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKKPFALTQPAKSLAPVAFAIALAGCSMAPKYDRPPAPIDIVYPSGAAYAEPAKATPEAPVTDAADIGWRDFFRDPLLQQ
LIGIALESNRDMRKAALNVEAAQALYRIQRAEVLPNLGVSGRGAAERLPADLSNTGAADVVRRYEVAGVTAAWELDVWGR
IRSLSNQALASYLAMDETRIATQMSLISEVANAYLTLRADQELLQLTGETLGTQKRSHELTAQLFEAGNATQLDVRRSEI
VLRTAEANFADYTRQTAKDRNALVLLLGQPLTLELSQQLDKAIVLPDGIVPTELPSGLPSELLVRRPDVRAAEQVLLGAN
ASIGAARAAFFPTISLTGSAGTASASLDGLFESGSRAWSFLPQITVPIFRGGALRANLDVAHVKKRIEIANYEKSIQVAF
SEVADGLAGKRTLDEQIRSEQFLVAASQGMYDLAEQRFREGVDDNLTLLDAQRTLYSAQQTLVRTRLARLSNLIALYKAL
GGGWTESTPSGVAQASR

Sequences:

>Translated_497_residues
MKKPFALTQPAKSLAPVAFAIALAGCSMAPKYDRPPAPIDIVYPSGAAYAEPAKATPEAPVTDAADIGWRDFFRDPLLQQ
LIGIALESNRDMRKAALNVEAAQALYRIQRAEVLPNLGVSGRGAAERLPADLSNTGAADVVRRYEVAGVTAAWELDVWGR
IRSLSNQALASYLAMDETRIATQMSLISEVANAYLTLRADQELLQLTGETLGTQKRSHELTAQLFEAGNATQLDVRRSEI
VLRTAEANFADYTRQTAKDRNALVLLLGQPLTLELSQQLDKAIVLPDGIVPTELPSGLPSELLVRRPDVRAAEQVLLGAN
ASIGAARAAFFPTISLTGSAGTASASLDGLFESGSRAWSFLPQITVPIFRGGALRANLDVAHVKKRIEIANYEKSIQVAF
SEVADGLAGKRTLDEQIRSEQFLVAASQGMYDLAEQRFREGVDDNLTLLDAQRTLYSAQQTLVRTRLARLSNLIALYKAL
GGGWTESTPSGVAQASR
>Mature_497_residues
MKKPFALTQPAKSLAPVAFAIALAGCSMAPKYDRPPAPIDIVYPSGAAYAEPAKATPEAPVTDAADIGWRDFFRDPLLQQ
LIGIALESNRDMRKAALNVEAAQALYRIQRAEVLPNLGVSGRGAAERLPADLSNTGAADVVRRYEVAGVTAAWELDVWGR
IRSLSNQALASYLAMDETRIATQMSLISEVANAYLTLRADQELLQLTGETLGTQKRSHELTAQLFEAGNATQLDVRRSEI
VLRTAEANFADYTRQTAKDRNALVLLLGQPLTLELSQQLDKAIVLPDGIVPTELPSGLPSELLVRRPDVRAAEQVLLGAN
ASIGAARAAFFPTISLTGSAGTASASLDGLFESGSRAWSFLPQITVPIFRGGALRANLDVAHVKKRIEIANYEKSIQVAF
SEVADGLAGKRTLDEQIRSEQFLVAASQGMYDLAEQRFREGVDDNLTLLDAQRTLYSAQQTLVRTRLARLSNLIALYKAL
GGGWTESTPSGVAQASR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=478, Percent_Identity=36.8200836820084, Blast_Score=295, Evalue=7e-81,
Organism=Escherichia coli, GI2367348, Length=435, Percent_Identity=26.4367816091954, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53585; Mature: 53585

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPFALTQPAKSLAPVAFAIALAGCSMAPKYDRPPAPIDIVYPSGAAYAEPAKATPEAP
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
VTDAADIGWRDFFRDPLLQQLIGIALESNRDMRKAALNVEAAQALYRIQRAEVLPNLGVS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRGAAERLPADLSNTGAADVVRRYEVAGVTAAWELDVWGRIRSLSNQALASYLAMDETRI
CCCHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATQMSLISEVANAYLTLRADQELLQLTGETLGTQKRSHELTAQLFEAGNATQLDVRRSEI
HHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCEE
VLRTAEANFADYTRQTAKDRNALVLLLGQPLTLELSQQLDKAIVLPDGIVPTELPSGLPS
EEEECCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCH
ELLVRRPDVRAAEQVLLGANASIGAARAAFFPTISLTGSAGTASASLDGLFESGSRAWSF
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCHHHHH
LPQITVPIFRGGALRANLDVAHVKKRIEIANYEKSIQVAFSEVADGLAGKRTLDEQIRSE
CCHHHHHEECCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
QFLVAASQGMYDLAEQRFREGVDDNLTLLDAQRTLYSAQQTLVRTRLARLSNLIALYKAL
CEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGWTESTPSGVAQASR
CCCCCCCCCCCHHCCCC
>Mature Secondary Structure
MKKPFALTQPAKSLAPVAFAIALAGCSMAPKYDRPPAPIDIVYPSGAAYAEPAKATPEAP
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCC
VTDAADIGWRDFFRDPLLQQLIGIALESNRDMRKAALNVEAAQALYRIQRAEVLPNLGVS
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GRGAAERLPADLSNTGAADVVRRYEVAGVTAAWELDVWGRIRSLSNQALASYLAMDETRI
CCCHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
ATQMSLISEVANAYLTLRADQELLQLTGETLGTQKRSHELTAQLFEAGNATQLDVRRSEI
HHHHHHHHHHHHHEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCEE
VLRTAEANFADYTRQTAKDRNALVLLLGQPLTLELSQQLDKAIVLPDGIVPTELPSGLPS
EEEECCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCEEECCCCCCCCCCCCCCCH
ELLVRRPDVRAAEQVLLGANASIGAARAAFFPTISLTGSAGTASASLDGLFESGSRAWSF
HHHHCCCCHHHHHHHHHCCCCCCCHHHHHHCCEEEECCCCCCCCHHHHHHHHCCCHHHHH
LPQITVPIFRGGALRANLDVAHVKKRIEIANYEKSIQVAFSEVADGLAGKRTLDEQIRSE
CCHHHHHEECCCCEEECCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHCCC
QFLVAASQGMYDLAEQRFREGVDDNLTLLDAQRTLYSAQQTLVRTRLARLSNLIALYKAL
CEEEEECCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGGWTESTPSGVAQASR
CCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]