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The map label for this gene is pnp [H]

Identifier: 120597905

GI number: 120597905

Start: 1222201

End: 1224300

Strand: Direct

Name: pnp [H]

Synonym: Sputw3181_1075

Alternate gene names: 120597905

Gene position: 1222201-1224300 (Clockwise)

Preceding gene: 120597903

Following gene: 120597906

Centisome position: 25.96

GC content: 44.43

Gene sequence:

>2100_bases
GTGAATCCAATTGTAAAGAGTTTTGAGTATGGTCAACATACAGTCACCCTGGAAACAGGCGTTATAGCGCGTCAAGCTGA
TGCCGCTGTGTTAGCAAGTATGGGTGACACAACAGTGTTAGTCACTGTTGTTGGTAAAAAAGAAGCCGAAGCCGGTCGTG
ATTTTTTCCCTCTGACTGTTAACTATCAAGAAAAAACCTACGCAGCAGGTAAAATTCCTGGCGGTTTCTTTAAGCGTGAG
GGTCGTCCTTCAGAAGATGAGACATTAATTGCTCGTCTTATTGACCGCCCAATTCGTCCTCTTTTCCCTAATGGTTTCAA
AAACGAAGTTCAAGTTATCATCACAGTTGTATCTGTCGACCCACAAATTGAGCCAGATATCATCTCTATGATTGGTACTT
CTGCTGCATTGGCCATTTCTGGTATCCCGTTTAGCGGTCCTTTGGGCGCAGCTCGTGTCGGTTATATCAATGGTGAATAT
GTATTGAACCCAACAGTGGCTCAAATTGAAACGAGCCAATTGAACTTAGTGGTTGCTGGTACAGCAAGTGCAGTGTTAAT
GGTGGAATCAGAAGCCCAAGCATTACCAGAAGAAGTGATGTTAGGTTCTGTTGTTTATGGTCACGATCAACAACAAGTGG
TTATTAACGCGATTGCTGAGTTTAAAGCTGAAGCGGGTAAACCAATGTGGGATTGGACTGCGCCAGTGCAAGATGAAACG
CTTGTTGCTCAAATTAAAGAGTTGGCTGAAGCGGGTTTAGGCGATGCGTATAAAATCCAAGTTAAGCAAGATCGTTATGC
TCAAGTTGCTGTAGTTAAAGCTGCAACGAAAGAAGCGTTATTAGCAAGCAATCCAAATATTGATTTACGTGAAGTAGACA
ATTTACTGGGCAGCTTAGAGAAGAAAGTCGTTCGTGGTCGTATTATTCGTGGCGAGCCGCGTATCGATGGTCGTGAACCC
GATATGATCCGTGCGTTAAGTGTATTAGCGGGCGTATTGCCACGTACTCACGGTAGCGCCTTGTTCACTCGCGGCGAAAC
CCAAGCCTTAGTAACTTGTACTTTAGGTACTGAACGTGATGCACAGAAGATCGATAGCATCATGGGTGAGCGCACTAATC
GTTTTATGTTGCACTATAACTTCCCTCCTTACTCTGTTGGTGAAACCGGCATGGTGGGTTCACCTAAGCGCCGCGAAATC
GGTCATGGAAAGTTAGCATGGCGTGGTATTAACGCTGTGATGCCATCGGCAGAAGAATTTCCATACAGCGTGCGCGTTGT
ATCTGAAATTACTGAATCTAATGGTTCAAGTTCTATGGCGTCTGTCTGTGGTACTTCCCTTGCGCTGATGGATGCGGGTG
TACCTATCAAGACTTCTGTAGCGGGTATTGCTATGGGCCTTGTAAAAGAAGGCGATGATTTTGTCGTACTTTCAGACATC
TTAGGTGATGAAGATCATTTAGGTGATATGGACTTTAAAGTGGCGGGTACCCGTGGTGGTATTACTGCACTGCAAATGGA
TATCAAGATCGAAGGTATCACCAAAGAAATTATGGAAATTGCACTACAACAAGCCTATGGCGCACGTGTGCATATTCTAA
ATGTGATGGATCAAGCGATTGGTTCGCACCGTGATGATATTTCTGATCATGCTCCACGTATCACTACAATCAAAATTAAC
CCAGAGAAAATCCGTGATGTTATCGGTAAAGGTGGCGCAGTTATTCGTGCTCTGACCGAAGAAACTGGCACTACGATTGA
GCTGGAAGATGATGGTACGGTAAGAATTGCCTCTTCAAATGGTGAAGCGACCAAAGAAGCTATCCGCCGTATTGAAGAAA
TCACTTCAGAAGTCGAAGTGGGCCGTATCTATAATGGTAAAGTGATCCGTATCGTTGATTTCGGTGCATTCGTGAACATT
CTGCCGGGCAAAGATGGTTTAGTACACATTTCACAAATCAGTGATGAACGTGTGGCTAACGTGTCTGATCACCTTGAACT
TAACCAAGAAGTTGCTGTTAAGGTAATGGAAGTGGATCGTCAAGGTCGTGTACGCTTGTCAATCAAAGAAGCACAAACGA
AAGAAGCTGCAGCAGAATAA

Upstream 100 bases:

>100_bases
AGATAGGTCTTTGTCATCATTATCTGCTCTTTATCTATTGTCCTTAATTGCAGTATACTTACGCGCGTAATTCAAGGTCA
TTAATTAAGGAATGGGTCAC

Downstream 100 bases:

>100_bases
CTGAGCCATAGGCTCAATATTCCATGTGCTTTTAAGTGATTGCATAGAAAGGAGATCAATTGATCTCCTTTTTCTTGGGT
CGAATTTAATATAATTGTGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVAQIETSQLNLVVAGTASAVLMVESEAQALPEEVMLGSVVYGHDQQQVVINAIAEFKAEAGKPMWDWTAPVQDET
LVAQIKELAEAGLGDAYKIQVKQDRYAQVAVVKAATKEALLASNPNIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRGGITALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVRIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKEAAAE

Sequences:

>Translated_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVAQIETSQLNLVVAGTASAVLMVESEAQALPEEVMLGSVVYGHDQQQVVINAIAEFKAEAGKPMWDWTAPVQDET
LVAQIKELAEAGLGDAYKIQVKQDRYAQVAVVKAATKEALLASNPNIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRGGITALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVRIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKEAAAE
>Mature_699_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEY
VLNPTVAQIETSQLNLVVAGTASAVLMVESEAQALPEEVMLGSVVYGHDQQQVVINAIAEFKAEAGKPMWDWTAPVQDET
LVAQIKELAEAGLGDAYKIQVKQDRYAQVAVVKAATKEALLASNPNIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAEEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRGGITALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITTIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVRIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKEAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=38.0084151472651, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=73.5166425470333, Blast_Score=1045, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=680, Percent_Identity=33.0882352941176, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=36.7088607594937, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=36.7088607594937, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=36.7088607594937, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.3774733637747, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75454; Mature: 75454

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVAQIETSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHEEEECEEEEEEEE
TASAVLMVESEAQALPEEVMLGSVVYGHDQQQVVINAIAEFKAEAGKPMWDWTAPVQDET
CCEEEEEEECHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEAGLGDAYKIQVKQDRYAQVAVVKAATKEALLASNPNIDLREVDNLLGSLE
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRGGITALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVRIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHHCCHHHEEEEEEECCCCCEEEEEEHHHCHHHCCC
>Mature Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEAEAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYINGEYVLNPTVAQIETSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHEEEECEEEEEEEE
TASAVLMVESEAQALPEEVMLGSVVYGHDQQQVVINAIAEFKAEAGKPMWDWTAPVQDET
CCEEEEEEECHHHHCCHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEAGLGDAYKIQVKQDRYAQVAVVKAATKEALLASNPNIDLREVDNLLGSLE
HHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAEEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHCCCHHHC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
CEEEEEHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRGGITALQMDIKIEGITKEIMEIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCEEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITTIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVRIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKEAAAE
CHHHHHCCHHHEEEEEEECCCCCEEEEEEHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA