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The map label for this gene is engD [H]

Identifier: 120597882

GI number: 120597882

Start: 1196025

End: 1197116

Strand: Direct

Name: engD [H]

Synonym: Sputw3181_1052

Alternate gene names: 120597882

Gene position: 1196025-1197116 (Clockwise)

Preceding gene: 120597881

Following gene: 120597884

Centisome position: 25.4

GC content: 45.6

Gene sequence:

>1092_bases
ATGGGTTTTAAATGCGGCATTGTTGGTCTTCCTAACGTAGGCAAATCAACGCTTTTTAATGCGTTAACTAAGGCTGGCAT
CGAAGCGGCTAACTTCCCGTTTTGTACTATTGAACCTAACACGGGTGTGGTACCTGTGCCAGATCCACGTTTGGATGCGT
TAGCTGCAATTGTCAATCCCCAACGTGTATTGCCAACAACGATGGAGTTTGTGGATATCGCAGGCTTAGTTGCTGGCGCA
TCTAAAGGCGAAGGTTTAGGTAACAAGTTCTTAGCTAACATCCGTGAAACCGATGCAATCGGTCATGTTGTGCGCTGTTT
TGATGATGACAACATTGTTCACGTGGCCAATAAGGTTGATCCTGCGGGCGATATTGAAGTGATTAACACTGAATTAGCCT
TGGCTGACTTAGACAGTTTAGAGCGTGCCGTTCTGCGTCAGCAAAAACGGGCTAAAGGTGGCGATAAAGACGCTAAGTTT
GAAGTGGAAGTATTAGAAAAAATGCGTCCAGTGCTCGATGAAGCCAAAATGTTGCGTTCAATGGATTTATCAAAAGAAGA
ATTGGAAGCCGTTGCTTACCTTAACTTCTTAACCTTAAAACCGACAATGTACATTGCCAACGTGGCGGAAGATGGTTTTG
AAAATAACCCGCATTTAGATGCAGTTCGCGCCATTGCGGCGACAGAAAATGCCATTGTTGTTGCCGTGTGTGCGGCGATT
GAATCTGAACTGGCTGAGATGGAAATCGAGGAGCGCGATGAGTTTATGGCCGATCTTGGGTTAGAAGAGCCAGGTTTAGA
TCGCGTTATTCGTGCGGGATACCAGTTACTTAATCTGCAAACGTACTTTACTGCTGGCGTAAAAGAGGTTCGCGCTTGGA
CTATTCCTGTAGGAGCGAGTGCTCCACAAGCTGCGGGCGTTATTCATACTGACTTTGAACGTGGCTTTATTCGTGCGCAG
GTGATGGCGTTTGAAGACTTCATTACCTACAAAGGGGAAGCTGGTGCCAAAGAAGCGGGCAAACTACGCGTTGAAGGTAA
AACTTATATCGTGAAAGATGGCGATGTTATGCATTTCTTGTTTAACGTATAA

Upstream 100 bases:

>100_bases
ACAGTTTTATGATTCAGTCATGTGTTGCCATTGGGTAGCACAACTAAAACTCGCTGGTGAGTGTGACTTGCTAGCTAATA
ATTGAGAGAAAGGTAAGCAT

Downstream 100 bases:

>100_bases
GCACGATTATTCAATGGCTAAGCATTCGTAAATTAGACTAGATAAGGGCAATAGGGCGCAATTTAGCGTATCTGTTGCTC
AGTTTGGTGAAGTAATAGCC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPVPDPRLDALAAIVNPQRVLPTTMEFVDIAGLVAGA
SKGEGLGNKFLANIRETDAIGHVVRCFDDDNIVHVANKVDPAGDIEVINTELALADLDSLERAVLRQQKRAKGGDKDAKF
EVEVLEKMRPVLDEAKMLRSMDLSKEELEAVAYLNFLTLKPTMYIANVAEDGFENNPHLDAVRAIAATENAIVVAVCAAI
ESELAEMEIEERDEFMADLGLEEPGLDRVIRAGYQLLNLQTYFTAGVKEVRAWTIPVGASAPQAAGVIHTDFERGFIRAQ
VMAFEDFITYKGEAGAKEAGKLRVEGKTYIVKDGDVMHFLFNV

Sequences:

>Translated_363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPVPDPRLDALAAIVNPQRVLPTTMEFVDIAGLVAGA
SKGEGLGNKFLANIRETDAIGHVVRCFDDDNIVHVANKVDPAGDIEVINTELALADLDSLERAVLRQQKRAKGGDKDAKF
EVEVLEKMRPVLDEAKMLRSMDLSKEELEAVAYLNFLTLKPTMYIANVAEDGFENNPHLDAVRAIAATENAIVVAVCAAI
ESELAEMEIEERDEFMADLGLEEPGLDRVIRAGYQLLNLQTYFTAGVKEVRAWTIPVGASAPQAAGVIHTDFERGFIRAQ
VMAFEDFITYKGEAGAKEAGKLRVEGKTYIVKDGDVMHFLFNV
>Mature_362_residues
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPVPDPRLDALAAIVNPQRVLPTTMEFVDIAGLVAGAS
KGEGLGNKFLANIRETDAIGHVVRCFDDDNIVHVANKVDPAGDIEVINTELALADLDSLERAVLRQQKRAKGGDKDAKFE
VEVLEKMRPVLDEAKMLRSMDLSKEELEAVAYLNFLTLKPTMYIANVAEDGFENNPHLDAVRAIAATENAIVVAVCAAIE
SELAEMEIEERDEFMADLGLEEPGLDRVIRAGYQLLNLQTYFTAGVKEVRAWTIPVGASAPQAAGVIHTDFERGFIRAQV
MAFEDFITYKGEAGAKEAGKLRVEGKTYIVKDGDVMHFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=374, Percent_Identity=44.6524064171123, Blast_Score=306, Evalue=1e-83,
Organism=Homo sapiens, GI58761502, Length=233, Percent_Identity=39.4849785407725, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=80.7162534435262, Blast_Score=609, Evalue=1e-176,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=33.0708661417323, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=43.2876712328767, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6319499, Length=368, Percent_Identity=40.2173913043478, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6321773, Length=379, Percent_Identity=35.3562005277045, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6321649, Length=165, Percent_Identity=36.969696969697, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640873, Length=372, Percent_Identity=44.0860215053763, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24640877, Length=372, Percent_Identity=44.0860215053763, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24640875, Length=372, Percent_Identity=44.0860215053763, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24640879, Length=334, Percent_Identity=41.9161676646707, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24585318, Length=197, Percent_Identity=29.4416243654822, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39441; Mature: 39309

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPVPDPRLDALAAIVNP
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHCCC
QRVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGHVVRCFDDDNIVHVANKVD
CHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCC
PAGDIEVINTELALADLDSLERAVLRQQKRAKGGDKDAKFEVEVLEKMRPVLDEAKMLRS
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
MDLSKEELEAVAYLNFLTLKPTMYIANVAEDGFENNPHLDAVRAIAATENAIVVAVCAAI
CCCCHHHHHHHHHHHHEEECCHHEEEEHHHCCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
ESELAEMEIEERDEFMADLGLEEPGLDRVIRAGYQLLNLQTYFTAGVKEVRAWTIPVGAS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCCC
APQAAGVIHTDFERGFIRAQVMAFEDFITYKGEAGAKEAGKLRVEGKTYIVKDGDVMHFL
CCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCEEEEE
FNV
ECC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPVPDPRLDALAAIVNP
CEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCHHHHHHHHCCC
QRVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGHVVRCFDDDNIVHVANKVD
CHHCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCEEEEECCCC
PAGDIEVINTELALADLDSLERAVLRQQKRAKGGDKDAKFEVEVLEKMRPVLDEAKMLRS
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
MDLSKEELEAVAYLNFLTLKPTMYIANVAEDGFENNPHLDAVRAIAATENAIVVAVCAAI
CCCCHHHHHHHHHHHHEEECCHHEEEEHHHCCCCCCCCHHHHHHHHHCCCEEEEHHHHHH
ESELAEMEIEERDEFMADLGLEEPGLDRVIRAGYQLLNLQTYFTAGVKEVRAWTIPVGAS
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEEEEEECCCC
APQAAGVIHTDFERGFIRAQVMAFEDFITYKGEAGAKEAGKLRVEGKTYIVKDGDVMHFL
CCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEECCCEEEEE
FNV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]