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The map label for this gene is cobS

Identifier: 120597817

GI number: 120597817

Start: 1126307

End: 1127095

Strand: Direct

Name: cobS

Synonym: Sputw3181_0987

Alternate gene names: 120597817

Gene position: 1126307-1127095 (Clockwise)

Preceding gene: 120597816

Following gene: 120597818

Centisome position: 23.92

GC content: 45.75

Gene sequence:

>789_bases
ATGTCAGAACGCGAAAGCTGGCACAAAGAAATAGATTTATTTTTAGTTGCTATGGGCTACTTTACCCGCATCCCAATGCC
TAAATGGGTTGAGGTTGATAGCGATAAACTCAATAAGGCGAGCCGTTATTTTGGCTTAGTGGGCCTGTTAATTGGCCTGT
TAAGTGCGATTGTCTTTTGGTTAACACAGAATTGGCTTCCTGCAGGGGTATCAGTGCTGTTAGCCATGTTAGTTGGCGTT
TTACTGACAGGTGGATTCCACGAGGATGGCCTAGCCGATACCTTCGACGGTTTTGGCGGTGGTTGGACGGCAGAAGATAA
ACTGCGGATCATGAAGGATTCGCGGCTTGGTAGTTATGGCGCTATCGCCTTGATATTAGCGTTATTGCTGAAATGGCAAT
TGTTGGTTGAATTGGCACTTTATGATCCCGTGGTTGCAGGCTCGGCGCTTATCGTGGCGCATACAGTGAGTCGCGTGGTT
GCTGCGAGCATTATTTTTACCGAAAAATATGTGCGCGATGATGAAACCAGCAAATCTAAACCACTATCTCAGCATCAAGG
CATTAATGAGCTATTTATCTTGGTTGCCAGCGGTGTATTGGTGTTGTTATTCCTTAAGGGATTAGCAGCCCTCTCATTGT
TGTTGGTGATGATCGGGTTACGCCGTTTGATTGTCGTTATTTTTAGGCGTCAGATTGGGGGATACACGGGCGATACTTTA
GGCGCGGCACAACAAATATGCGAGATTGTTTGTTATCTTGTGCTGCTGATTGTTGGAAGTATTCTGTGA

Upstream 100 bases:

>100_bases
CAGCTAAGCTGTTTATTTTACTTATTTAAAATAATAACTTTTTAGCCAACCGTTTTAATTTTAAACCTTGCAGCAAGTGT
GGATTCACTAAGGATGCTTT

Downstream 100 bases:

>100_bases
TCCATTTAGTCGTAGGAGGTGCCCGTAGCGGCAAGAGCCGTTACGGCGAATCCTTAGTGCGCCAATATACGGCGCTGGGA
TTTGATGCGTGCTTTGTTGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLIGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGAIALILALLLKWQLLVELALYDPVVAGSALIVAHTVSRVV
AASIIFTEKYVRDDETSKSKPLSQHQGINELFILVASGVLVLLFLKGLAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
GAAQQICEIVCYLVLLIVGSIL

Sequences:

>Translated_262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLIGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGAIALILALLLKWQLLVELALYDPVVAGSALIVAHTVSRVV
AASIIFTEKYVRDDETSKSKPLSQHQGINELFILVASGVLVLLFLKGLAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
GAAQQICEIVCYLVLLIVGSIL
>Mature_261_residues
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLIGLLSAIVFWLTQNWLPAGVSVLLAMLVGVL
LTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGAIALILALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVA
ASIIFTEKYVRDDETSKSKPLSQHQGINELFILVASGVLVLLFLKGLAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTLG
AAQQICEIVCYLVLLIVGSIL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=36.0323886639676, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28663; Mature: 28531

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLIGLLSAIVFW
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLAMLVGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
AIALILALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVAASIIFTEKYVRDDETSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLSQHQGINELFILVASGVLVLLFLKGLAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQICEIVCYLVLLIVGSIL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SERESWHKEIDLFLVAMGYFTRIPMPKWVEVDSDKLNKASRYFGLVGLLIGLLSAIVFW
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLAMLVGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
AIALILALLLKWQLLVELALYDPVVAGSALIVAHTVSRVVAASIIFTEKYVRDDETSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLSQHQGINELFILVASGVLVLLFLKGLAALSLLLVMIGLRRLIVVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQICEIVCYLVLLIVGSIL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA