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The map label for this gene is proA

Identifier: 120597802

GI number: 120597802

Start: 1104842

End: 1106110

Strand: Direct

Name: proA

Synonym: Sputw3181_0972

Alternate gene names: 120597802

Gene position: 1104842-1106110 (Clockwise)

Preceding gene: 120597801

Following gene: 120597806

Centisome position: 23.47

GC content: 50.2

Gene sequence:

>1269_bases
ATGAATCAAGCGCAGTATTTACAGCAACTTGGCGCTAATGCCAAACAGGCAAGTTATGCCTTAGCCAACTTAACGGCGAG
TCAAAAGGCCGATTTGCTCGAGGCTATTGCCGATGCGCTGACGGAAAACTCTCCGGCCATTTTAGCGGCTAACGCTAAGG
ATGTTGCCGCTGCTAAGGCCGATGGATTAACGGAGGCGATGATCGACAGATTATTGCTCGATGATGCGCGCCTTGCGGGC
ATTATCGGTGACATCAGCGATGTCATTCGCCTTGCCGATCCCGTCGGTGAAGAATTTGGCAGTAAGTTATTGGATAACGG
GTTAAGGCTGACTCGCCGCCGTGTACCGCTTGGGGTGATTGGGGTGATTTATGAAGCACGGCCTAATGTGACCGTGGATA
TCGCGGTGCTTGCCCTTAAAACTGGTAATGCGGTGATCCTCCGTGGCGGTAAAGAAACCCTTGAATCGAATAAACTTATT
AGTGATGTGATCCGCAGCGCGATTGTCGCCCAAGGTTTGCCCATAGATGCGGTGCAATTTATTGATTCGCCTGATAGAGC
CTTAGTGACGGGACTGCTAAAGCTCGATCAATATGTTGACATGATAGTGCCGCGCGGCGGTCAAGCTCTGCAGCGCCTAT
GTGCTGAGCAAGCGACCATTCCGGTGATCTTAGGCGGCATTGGCATTTGCCATTTATATGTCGATAAAAATGCTGACTTA
CAACGTGCCCTCGATGTTATTGCTAACGCCAAAGTGCAGCGTCCGACCGTGTGTAATGCGCTCGATACCTTGCTGGTGGA
TGAGCGCGTGGCGAGCAGCTTTGTACCGCAAGTTGCCGAGTATTTGCATCGTTTAGGTGTGCGTTTTTCAGTCTGTGAGA
CAAGTTTTGCCCTTCTCGATGGTTTAGGGTTTGATGTGATGCTTGCTCAGTCTGAGGATTTTGGCATTGAATGGTTGTCA
CTCACCTTAGGCATCAAAGTCGTGGCTGATATCGATACTGCCGTTGACCATATTCGTACTTATTCTAGTGGGCATTCGGA
AGCGATTCTGACCGATGATATTCATGCTGCGGCGCACTTTATGAACCAAGTGAATTCAGCCGCTGTGTATGTCAATGCCA
GCACGCGTTTTACTGATGGTGGCCAGTTTGGTTTAGGTGCCGAAGTTGCGGTAAGCACGCAAAAGTTGCACGCCCGCGGC
CCAATGGGGCTTGAGGCATTAACCACCTATAAATGGTTAGCTTGGGGCGATTACACCAGCCGTATTTAA

Upstream 100 bases:

>100_bases
TTCCGACGAGATCGAGTCAGTACTCGGTTACGATTATGGTGACGCAATAGTGCACCGAAACGACATGGTGGTCCTATAAG
CGAGGTCTGTTTTGAGTGAA

Downstream 100 bases:

>100_bases
AAGACGGTAAACAAAAGCTATTGATGGATAACATCAATGGCTTTTTATTGTGAATATGCGCGATTAAATCTTTTGTGGAT
AGGTTTAGCGAATAAGAGGG

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNQAQYLQQLGANAKQASYALANLTASQKADLLEAIADALTENSPAILAANAKDVAAAKADGLTEAMIDRLLLDDARLAG
IIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLI
SDVIRSAIVAQGLPIDAVQFIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNADL
QRALDVIANAKVQRPTVCNALDTLLVDERVASSFVPQVAEYLHRLGVRFSVCETSFALLDGLGFDVMLAQSEDFGIEWLS
LTLGIKVVADIDTAVDHIRTYSSGHSEAILTDDIHAAAHFMNQVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARG
PMGLEALTTYKWLAWGDYTSRI

Sequences:

>Translated_422_residues
MNQAQYLQQLGANAKQASYALANLTASQKADLLEAIADALTENSPAILAANAKDVAAAKADGLTEAMIDRLLLDDARLAG
IIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLI
SDVIRSAIVAQGLPIDAVQFIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNADL
QRALDVIANAKVQRPTVCNALDTLLVDERVASSFVPQVAEYLHRLGVRFSVCETSFALLDGLGFDVMLAQSEDFGIEWLS
LTLGIKVVADIDTAVDHIRTYSSGHSEAILTDDIHAAAHFMNQVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARG
PMGLEALTTYKWLAWGDYTSRI
>Mature_422_residues
MNQAQYLQQLGANAKQASYALANLTASQKADLLEAIADALTENSPAILAANAKDVAAAKADGLTEAMIDRLLLDDARLAG
IIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLI
SDVIRSAIVAQGLPIDAVQFIDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNADL
QRALDVIANAKVQRPTVCNALDTLLVDERVASSFVPQVAEYLHRLGVRFSVCETSFALLDGLGFDVMLAQSEDFGIEWLS
LTLGIKVVADIDTAVDHIRTYSSGHSEAILTDDIHAAAHFMNQVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARG
PMGLEALTTYKWLAWGDYTSRI

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=412, Percent_Identity=35.9223300970874, Blast_Score=246, Evalue=4e-65,
Organism=Homo sapiens, GI21361368, Length=412, Percent_Identity=35.9223300970874, Blast_Score=246, Evalue=4e-65,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=58.7951807228916, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=35.1807228915663, Blast_Score=251, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=35.1807228915663, Blast_Score=251, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=38.2830626450116, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI21357643, Length=410, Percent_Identity=36.8292682926829, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45143; Mature: 45143

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAQYLQQLGANAKQASYALANLTASQKADLLEAIADALTENSPAILAANAKDVAAAKA
CCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
DGLTEAMIDRLLLDDARLAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEE
GVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISDVIRSAIVAQGLPIDAVQF
EEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNADL
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCH
QRALDVIANAKVQRPTVCNALDTLLVDERVASSFVPQVAEYLHRLGVRFSVCETSFALLD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHH
GLGFDVMLAQSEDFGIEWLSLTLGIKVVADIDTAVDHIRTYSSGHSEAILTDDIHAAAHF
CCCCEEEEECCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
MNQVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGDYTS
HHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECCHHC
RI
CC
>Mature Secondary Structure
MNQAQYLQQLGANAKQASYALANLTASQKADLLEAIADALTENSPAILAANAKDVAAAKA
CCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
DGLTEAMIDRLLLDDARLAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVI
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEE
GVIYEARPNVTVDIAVLALKTGNAVILRGGKETLESNKLISDVIRSAIVAQGLPIDAVQF
EEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IDSPDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNADL
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCH
QRALDVIANAKVQRPTVCNALDTLLVDERVASSFVPQVAEYLHRLGVRFSVCETSFALLD
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHH
GLGFDVMLAQSEDFGIEWLSLTLGIKVVADIDTAVDHIRTYSSGHSEAILTDDIHAAAHF
CCCCEEEEECCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHH
MNQVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGDYTS
HHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHHEEECCHHC
RI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]