Click here to switch to the map view.

The map label for this gene is lysS

Identifier: 120597726

GI number: 120597726

Start: 1021858

End: 1023360

Strand: Direct

Name: lysS

Synonym: Sputw3181_0896

Alternate gene names: 120597726

Gene position: 1021858-1023360 (Clockwise)

Preceding gene: 120597725

Following gene: 120597728

Centisome position: 21.7

GC content: 47.84

Gene sequence:

>1503_bases
ATGACTGAACAAGTACAAGATGAAAACAAACTGATCGCCGAGCGTCGTGCCAAGCTAGACAGCATTCGCCCAAATTGTAG
CGCTAATGCTCACCCTAATACCTTCCGTCGCACCCATAAAGCGGCTGAGTTACAGGAGAAATACGGCCAAAACACTAAGG
AAGAACTCGAAGCCTTAGGTTTTAGAACCAGCATTGCTGGTCGTATCATGGCAAAACGTGGCCCATTCCTCGTAATTCAA
GACGTGTCTGGTCGTATCCAAGCTTATGCTGAAAAGGGCGTTCAAGCGGATCTGAAGGATCGTTACCAAGGTCTGGATAT
CGGTGACATCATCGGCGTAACTGGTCAGTTACACTTGTCTGGTAAAGGCGATCTGTACGTCAATATGGAAGAGTACCAAC
TGCTGACCAAAGCACTGCGTCCACTGCCAGAGAAATTCCACGGCTTAACCGACCAAGAAACCCGTTACCGCCAACGTTAT
GTTGACTTAATCGTTAACGAAGAATCTCGTCAAGCCTTCGTGATGCGCTCAAAAGTGGTTGCAGCTATCCGTAACTTCAT
GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCTGGCGGCGCTTCAGCCCGTCCATTTATCA
CGCACCACAATGCGCTGGACATGCCAATGTACCTGCGTATCGCGCCAGAGCTATACCTCAAGCGTTTAGTGGTCGGTGGT
TTTGAGCGTGTGTTCGAAATCAACCGTAACTTCCGTAACGAAGGTCTGTCGCCACGCCATAACCCAGAATTCACTATGAT
GGAATTCTATATGGCTTACGCTGATTACAAAGATTTGATGGACTTAACCGAAGAACTGTTAAGCTCAATCGCGATCGAAT
TGTTAGGCAGTGCGCAAATGCCATACGGCGAGCACACCGTTGATTTCGGCGGCCCATATGCCCGTCTGAGCATGTTAGAA
GCGATTCAGAAGTACAATCCTGATAACGCGACTATCCAAGCCATGACCTACGAGCAAGTGAAAGATCTCGAATTTATGCG
TGAATTGGCTATCAGCCTAGGTATCAAGATCGAGAAGTTCTGGACCTGTGGTCAGCTATTAGAAGAAATCTTCGGTGAAA
CTGCTGAATGGCAATTAATGCAACCGACCTTCATCACTGGCTACCCAGCGGACATTTCGCCACTGGCACGTCGTAACGAT
GACAATCACTTCATCACCGACCGTTTTGAATTCTTCATCGGTGGTCGTGAAGTAGCTAACGGTTTCAGCGAGTTAAATGA
TGCTGAAGATCAAGACAGCCGCTTTAAAGCGCAGGTTGATGCCAAAGACGCGGGTGACGATGAAGCCATGTTCTACGATG
CGGACTATATCACTGCACTGGAACACGGTTTACCACCAACAGCGGGTCAAGGTATTGGTATCGACCGTTTAGTGATGCTA
TTCACTAACACTCATACAATCCGTGATGTGATCCTATTCCCTGCAATGCGCCCACAAGCCTAA

Upstream 100 bases:

>100_bases
GCAATACCCAAGCGGTACTCGATGGCGATCTGGACAAGTTTATCGAAGCCAGTTTGAAATCAGGGCTGTAATCAATTCAA
AGGTATAGACGAGAAATAAC

Downstream 100 bases:

>100_bases
GTGATTCATTGGGTTGCAGATAAAACCTGCGACCCAATTTGCATTGGATAAATATAAAAACCAGCCTTTTGGCTGGTTTT
TATATGTGCGATTCAAGGGG

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFRTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

Sequences:

>Translated_500_residues
MTEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFRTSIAGRIMAKRGPFLVIQ
DVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLE
AIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRND
DNHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA
>Mature_499_residues
TEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALGFRTSIAGRIMAKRGPFLVIQD
VSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLSGKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYV
DLIVNEESRQAFVMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGGF
ERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQMPYGEHTVDFGGPYARLSMLEA
IQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKFWTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDD
NHFITDRFEFFIGGREVANGFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVMLF
TNTHTIRDVILFPAMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=513, Percent_Identity=40.7407407407407, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI194272210, Length=513, Percent_Identity=40.7407407407407, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI45439306, Length=350, Percent_Identity=24.5714285714286, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40789249, Length=254, Percent_Identity=26.3779527559055, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=61.9238476953908, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=502, Percent_Identity=60.3585657370518, Blast_Score=616, Evalue=1e-178,
Organism=Escherichia coli, GI87082379, Length=338, Percent_Identity=31.3609467455621, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788173, Length=276, Percent_Identity=25, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=511, Percent_Identity=41.6829745596869, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994340, Length=511, Percent_Identity=41.6829745596869, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535927, Length=505, Percent_Identity=41.5841584158416, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32566633, Length=304, Percent_Identity=27.3026315789474, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=362, Percent_Identity=25.6906077348066, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320242, Length=520, Percent_Identity=40.9615384615385, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=384, Percent_Identity=31.7708333333333, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=358, Percent_Identity=27.9329608938547, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6325153, Length=278, Percent_Identity=25.5395683453237, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24640849, Length=510, Percent_Identity=40.5882352941176, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24640851, Length=510, Percent_Identity=40.5882352941176, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24584738, Length=335, Percent_Identity=25.9701492537313, Blast_Score=83, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57062; Mature: 56931

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALG
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
FRTSIAGRIMAKRGPFLVIQDVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLS
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCEEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEESRQAFVMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHEEECHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHTVDFGGPYARLSMLEAIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
WTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQVQDENKLIAERRAKLDSIRPNCSANAHPNTFRRTHKAAELQEKYGQNTKEELEALG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHC
FRTSIAGRIMAKRGPFLVIQDVSGRIQAYAEKGVQADLKDRYQGLDIGDIIGVTGQLHLS
HHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHCCCCHHHHHCCCEEEEEC
GKGDLYVNMEEYQLLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEESRQAFVMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFITHHNALDMPMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECCCCCCCHHEEECHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLMDLTEELLSSIAIELLGSAQM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHTVDFGGPYARLSMLEAIQKYNPDNATIQAMTYEQVKDLEFMRELAISLGIKIEKF
CCCCCEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCHHHH
WTCGQLLEEIFGETAEWQLMQPTFITGYPADISPLARRNDDNHFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCHHHHCCCCCCEEHHHHHHEECCHHHHH
GFSELNDAEDQDSRFKAQVDAKDAGDDEAMFYDADYITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEECCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA