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The map label for this gene is prfB [H]

Identifier: 120597725

GI number: 120597725

Start: 1020770

End: 1021828

Strand: Direct

Name: prfB [H]

Synonym: Sputw3181_0895

Alternate gene names: 120597725

Gene position: 1020770-1021828 (Clockwise)

Preceding gene: 120597723

Following gene: 120597726

Centisome position: 21.68

GC content: 49.48

Gene sequence:

>1059_bases
TTGCCGAGCGTACGCAGCTTCTTAGGGGGTATCTTTGACTACGATGCTAAACATGAGCGTCTAGAAGAAGTTACCCGTGA
GCTTGAAAGTTCCGATGTGTGGAACGAGCCTGAGCGCGCCCAAGCCCTAGGCAAAGAGCGTGCAAGCCTCGAAGCCGTAG
TAAAAACAATCGACGATATGGACTCAGGTCTTGAGGATGTCGAAGGGCTACTCGAGCTGGCCGTTGAAGAAGAAGACGAA
GACACCTTCAATGAAACCACCAAAGAGCTGGATTATTTAGAAAGTCGCCTTGCTGATCTTGAATTTCGCCGTATGTTCTC
TGGTCCACAGGATGCCTCAAACTGTTACTTAGACATTCAGTCGGGCAGTGGCGGTACTGAGGCTCAAGATTGGGCCAACA
TGGTACTGCGTATGTACCTACGTTGGGGTGAAGCCCATGGATTCAGCCCTGAATTGATGGAAGTGACCGATGGTGACGTG
GCGGGCATTAAGGGTGCGACCATTAAATTCACTGGTGAATATGCTTTCGGTTGGTTACGCACCGAAACTGGCGTGCACCG
TTTAGTGCGTAAATCGCCATTTGACTCTTCGGGTCGTCGCCACACGTCTTTCTGCTCGGTATTTGTTTACCCTGAGATTG
ATGATGATATCACCATCGATATAAACCCTGCTGACTTACGTATCGACGTATACCGCGCCTCGGGTGCGGGTGGTCAGCAC
GTTAACCGAACCGAATCAGCGGTACGTATTACCCACTTACCGACCAACACGGTAGTGCAGTGTCAGAACGATCGTTCACA
GCACAAGAACAAAGATTCGGCGATGAAACAGTTAAAAGCCAAACTGTTCGAATTAGAAATGCATAAGCAAAATGCTGAAA
AGCAAGCGGCCGAAGACGCTAAATCTGACATCGGCTGGGGCAGCCAAATTCGGTCCTATGTATTGGACGATTCCCGTATT
AAAGACTTACGTACTAGCGTGGAGACGCGCAATACCCAAGCGGTACTCGATGGCGATCTGGACAAGTTTATCGAAGCCAG
TTTGAAATCAGGGCTGTAA

Upstream 100 bases:

>100_bases
CACCGCCTCCTTTAATGGGAAATCAATTAAAGCGGCCAAATAAAGTGAAGTTGAAAATTCATGTTTGAAGTTAATCCAGT
AAAATTCAAAATTAAGGAGC

Downstream 100 bases:

>100_bases
TCAATTCAAAGGTATAGACGAGAAATAACATGACTGAACAAGTACAAGATGAAAACAAACTGATCGCCGAGCGTCGTGCC
AAGCTAGACAGCATTCGCCC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPSVRSFLGGIFDYDAKHERLEEVTRELESSDVWNEPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAVEEEDE
DTFNETTKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRI
KDLRTSVETRNTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_352_residues
MPSVRSFLGGIFDYDAKHERLEEVTRELESSDVWNEPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAVEEEDE
DTFNETTKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRI
KDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
>Mature_351_residues
PSVRSFLGGIFDYDAKHERLEEVTRELESSDVWNEPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAVEEEDED
TFNETTKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVA
GIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQHV
NRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRIK
DLRTSVETRNTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=32.8990228013029, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI34577120, Length=321, Percent_Identity=31.7757009345794, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=34.1121495327103, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=78.8405797101449, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1787462, Length=344, Percent_Identity=38.3720930232558, Blast_Score=207, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=29.5373665480427, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=31.8611987381703, Blast_Score=142, Evalue=6e-35,
Organism=Drosophila melanogaster, GI19921226, Length=207, Percent_Identity=41.5458937198068, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39573; Mature: 39442

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVRSFLGGIFDYDAKHERLEEVTRELESSDVWNEPERAQALGKERASLEAVVKTIDDM
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAVEEEDEDTFNETTKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCCCC
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIGWGSQIRSYVLDDSRIKDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PSVRSFLGGIFDYDAKHERLEEVTRELESSDVWNEPERAQALGKERASLEAVVKTIDDM
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAVEEEDEDTFNETTKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPADLRIDVYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCEEEEEEEEECCCCCCC
VNRTESAVRITHLPTNTVVQCQNDRSQHKNKDSAMKQLKAKLFELEMHKQNAEKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSDIGWGSQIRSYVLDDSRIKDLRTSVETRNTQAVLDGDLDKFIEASLKSGL
HHCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA