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The map label for this gene is hldE [H]

Identifier: 120597706

GI number: 120597706

Start: 993008

End: 994438

Strand: Direct

Name: hldE [H]

Synonym: Sputw3181_0876

Alternate gene names: 120597706

Gene position: 993008-994438 (Clockwise)

Preceding gene: 120597704

Following gene: 120597707

Centisome position: 21.09

GC content: 49.69

Gene sequence:

>1431_bases
ATGAAGGTTTCTCTGCCAGCATTTGAAAAAGCCAGAGTGTTAGTCGTCGGCGATGTGATGTTAGACCGTTATTGGGTTGG
CCCTACGGGACGTATCTCCCCTGAGGCACCCGTACCTGTAGTGAAGATTAATCAGGTTGAAGACAGACCGGGTGGCGCGG
CCAACGTGGCCCTCAATATCGCAACCTTAGGTGGCCAAGTCCAACTTGCAGGGCTCGTTGGCCAAGATGATACCGCCATA
GCCTTAACACTCGGTGTGCAAACCTTAGGGGTTGAACCCCAATGGTTAAGTATTGCGGATAAACCAACTATCACTAAGTT
GCGGGTATTATCACGTAATCAGCAGCTTATTCGCCTCGATTTCGAAGAAGCCTTCGACAAGGCCGATAGCGTGCGTTTAC
TTAAGCAATCCGAAGCCTTACTCGACTCTATCGATGTGGTGGTGTTGTCCGACTATGCCAAGGGCGCTATCGATCAGCCG
CGAGATTTTATTGCCTTAGCGCGTTCTAAAGGTGTGATGGTATTAGTCGATCCTAAGGGCAGTGACTTTGGTCGTTATCA
TGGCGCGTCACTCATTACCCCAAATATGAGCGAGTTTGAAGCTGTTGTGGGTACGGTGACCTCTGAAGCCGATCTACTAG
AAAAAGCCCGTGGTTTACTCAAAGAACATCATTTCGATGCCATTTTAGTGACCCGCTCTGAAAAGGGCATGACCTTAGTG
ACTGCTAATGCGCCTGAATTACACATCCCAACCGTTGCCCGCGAAGTCTATGATGTGACGGGGGCTGGCGACACTGTGAT
TTCGGCCTTAGCAACCTCATTAGCCGCAGGGGCCGATTTACCCCAAGCTTGTGCTATTGCAAATACGGCTGCGGGTGTGG
TGGTCGGAAAACTTGGCACTTCAACCGTGAGTCGTATCGAGCTTATCGAAGCCTTAGCTTTGCACCATGGAGAATCGGGT
TTTGGTGTGGTGAGTGAAGATCAGCTTGCCTACGCCTTAGAGCAAGCCAAACTGCGCGGTGAACGTGTGGTGATGACCAA
TGGTTGTTTCGATATTTTGCATGCGGGTCACGTGAGTTACCTAAAGCAGGCTAAAGCTTTAGGTGATCGTTTAATTGTCG
CAGTAAACGATGATGCCTCGGTTAAACGCCTCAAGGGTGAAGGCCGTCCAGTTAATCAGGTTGATCGTCGTATGGCAGTG
CTCGCAGGCTTAGCTTCTGTCGATTGGGTGGTGCCTTTTAGTGAAGATACGCCGCAGCGGATCATCGCCAGATTGCTGCC
GGATCTCTTGGTCAAAGGTGGCGATTATAAAATTGAAGATATTGCGGGTGGCGCTGAAGTGATTGCCGCTGGTGGTCAAG
TACAAGTACTTGGGTTTGAAGATGGTATTTCAACAACGGCCATTATCCAAAATATTATGGCTAATCAGTGA

Upstream 100 bases:

>100_bases
AATACTCACTCGTTTTGAAAATTACTGCTTATTCTAACTCAATTTTTACTGAAAATTAGGTACAATCACGGTTAAATTTC
TGCATGAAGATAAGAGCGCT

Downstream 100 bases:

>100_bases
GTTTAATTGTTCTGACATTGGTTTCAAGCCTGTCTTGGGTGCCTATCGCGGATAAGCAGGCTTTAATTTGTCCTTTGTCG
GTGTTAAACAAATGTCTCGA

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKVSLPAFEKARVLVVGDVMLDRYWVGPTGRISPEAPVPVVKINQVEDRPGGAANVALNIATLGGQVQLAGLVGQDDTAI
ALTLGVQTLGVEPQWLSIADKPTITKLRVLSRNQQLIRLDFEEAFDKADSVRLLKQSEALLDSIDVVVLSDYAKGAIDQP
RDFIALARSKGVMVLVDPKGSDFGRYHGASLITPNMSEFEAVVGTVTSEADLLEKARGLLKEHHFDAILVTRSEKGMTLV
TANAPELHIPTVAREVYDVTGAGDTVISALATSLAAGADLPQACAIANTAAGVVVGKLGTSTVSRIELIEALALHHGESG
FGVVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLKQAKALGDRLIVAVNDDASVKRLKGEGRPVNQVDRRMAV
LAGLASVDWVVPFSEDTPQRIIARLLPDLLVKGGDYKIEDIAGGAEVIAAGGQVQVLGFEDGISTTAIIQNIMANQ

Sequences:

>Translated_476_residues
MKVSLPAFEKARVLVVGDVMLDRYWVGPTGRISPEAPVPVVKINQVEDRPGGAANVALNIATLGGQVQLAGLVGQDDTAI
ALTLGVQTLGVEPQWLSIADKPTITKLRVLSRNQQLIRLDFEEAFDKADSVRLLKQSEALLDSIDVVVLSDYAKGAIDQP
RDFIALARSKGVMVLVDPKGSDFGRYHGASLITPNMSEFEAVVGTVTSEADLLEKARGLLKEHHFDAILVTRSEKGMTLV
TANAPELHIPTVAREVYDVTGAGDTVISALATSLAAGADLPQACAIANTAAGVVVGKLGTSTVSRIELIEALALHHGESG
FGVVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLKQAKALGDRLIVAVNDDASVKRLKGEGRPVNQVDRRMAV
LAGLASVDWVVPFSEDTPQRIIARLLPDLLVKGGDYKIEDIAGGAEVIAAGGQVQVLGFEDGISTTAIIQNIMANQ
>Mature_476_residues
MKVSLPAFEKARVLVVGDVMLDRYWVGPTGRISPEAPVPVVKINQVEDRPGGAANVALNIATLGGQVQLAGLVGQDDTAI
ALTLGVQTLGVEPQWLSIADKPTITKLRVLSRNQQLIRLDFEEAFDKADSVRLLKQSEALLDSIDVVVLSDYAKGAIDQP
RDFIALARSKGVMVLVDPKGSDFGRYHGASLITPNMSEFEAVVGTVTSEADLLEKARGLLKEHHFDAILVTRSEKGMTLV
TANAPELHIPTVAREVYDVTGAGDTVISALATSLAAGADLPQACAIANTAAGVVVGKLGTSTVSRIELIEALALHHGESG
FGVVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSYLKQAKALGDRLIVAVNDDASVKRLKGEGRPVNQVDRRMAV
LAGLASVDWVVPFSEDTPQRIIARLLPDLLVKGGDYKIEDIAGGAEVIAAGGQVQVLGFEDGISTTAIIQNIMANQ

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=476, Percent_Identity=61.9747899159664, Blast_Score=583, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 50481; Mature: 50481

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSLPAFEKARVLVVGDVMLDRYWVGPTGRISPEAPVPVVKINQVEDRPGGAANVALNI
CCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
ATLGGQVQLAGLVGQDDTAIALTLGVQTLGVEPQWLSIADKPTITKLRVLSRNQQLIRLD
EEECCEEEEEEEECCCCCEEEEEECEEEECCCCCEEEECCCCCHHHHHHHCCCCEEEEEE
FEEAFDKADSVRLLKQSEALLDSIDVVVLSDYAKGAIDQPRDFIALARSKGVMVLVDPKG
HHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCC
SDFGRYHGASLITPNMSEFEAVVGTVTSEADLLEKARGLLKEHHFDAILVTRSEKGMTLV
CCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
TANAPELHIPTVAREVYDVTGAGDTVISALATSLAAGADLPQACAIANTAAGVVVGKLGT
ECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCC
STVSRIELIEALALHHGESGFGVVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSY
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
LKQAKALGDRLIVAVNDDASVKRLKGEGRPVNQVDRRMAVLAGLASVDWVVPFSEDTPQR
HHHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHH
IIARLLPDLLVKGGDYKIEDIAGGAEVIAAGGQVQVLGFEDGISTTAIIQNIMANQ
HHHHHHHHHHHCCCCEEEEECCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKVSLPAFEKARVLVVGDVMLDRYWVGPTGRISPEAPVPVVKINQVEDRPGGAANVALNI
CCCCCCCCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEEEE
ATLGGQVQLAGLVGQDDTAIALTLGVQTLGVEPQWLSIADKPTITKLRVLSRNQQLIRLD
EEECCEEEEEEEECCCCCEEEEEECEEEECCCCCEEEECCCCCHHHHHHHCCCCEEEEEE
FEEAFDKADSVRLLKQSEALLDSIDVVVLSDYAKGAIDQPRDFIALARSKGVMVLVDPKG
HHHHHCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHCCCCEEEEECCCC
SDFGRYHGASLITPNMSEFEAVVGTVTSEADLLEKARGLLKEHHFDAILVTRSEKGMTLV
CCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEE
TANAPELHIPTVAREVYDVTGAGDTVISALATSLAAGADLPQACAIANTAAGVVVGKLGT
ECCCCCEECCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECCCC
STVSRIELIEALALHHGESGFGVVSEDQLAYALEQAKLRGERVVMTNGCFDILHAGHVSY
HHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
LKQAKALGDRLIVAVNDDASVKRLKGEGRPVNQVDRRMAVLAGLASVDWVVPFSEDTPQR
HHHHHHCCCEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHH
IIARLLPDLLVKGGDYKIEDIAGGAEVIAAGGQVQVLGFEDGISTTAIIQNIMANQ
HHHHHHHHHHHCCCCEEEEECCCCCCEEECCCEEEEEEECCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA