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The map label for this gene is bla [H]

Identifier: 120597617

GI number: 120597617

Start: 878634

End: 879446

Strand: Direct

Name: bla [H]

Synonym: Sputw3181_0786

Alternate gene names: 120597617

Gene position: 878634-879446 (Clockwise)

Preceding gene: 120597606

Following gene: 120597619

Centisome position: 18.66

GC content: 44.53

Gene sequence:

>813_bases
ATGCGTTTGTTCGTCATCTCGGCGGTATTGGTGATGTCCTCCATCTCAGCTTCCCCTGTTTTTGCTGCTAGCTCGCCCCA
AAAAGAGTGGCAAGAAACCCGCAGTTTGGATGCCAGTTTTACTCAGCACCAAGCTAAAGGCGTGGTGGTGTTATGGAATG
AAAATAAACAACAGGGATTCACTAATAATCTTAAACGCGCCAATCAAGGTTTTTTACCTGCATCGACGTTTAAAATTCCC
AACAGTTTGATTGCTCTCGATTTAGGGATGGTGAAAGATGAGCACCAAGTTTTTAAGTGGGATGGTAAAAATCGTGATAT
TGCCGCCTGGAATCGTGACCATAATTTGATTAGCGCCATGAAGTATTCGGTTGTGCCCATCTACCAAGAGTTTGCTCGCC
AAATAGGTGATGCTCGCATGGGGAAGATGATCGCTGTTTTTGATTATGGCAATGAGGATATCTCAGGCAATTTAGACAGT
TTTTGGTTAGATGGAGGCATTCGGATCTCAGCCAAAGAGCAAATCGATTTCCTACGTCGGCTTTATCATAACAAGTTGCA
TGTTTCCGAGAGAAGTCAGCGTATTGTTAAGCAAGCCATGCTGACTGAAGCCAATAGTGACTATATTATCCGCGCAAAAA
CGGGTTATGCCGTAAGGGCCGAACCGAGCATTGGTTGGTGGGTCGGTTGGGTAGAACTCGATGATAATGTATGGTTTTTT
GCAATGAATATGGATATGCCATCAGCGGATGGTTTGGCACTGCGCCAAGCCATTACAAAAGAAGTACTCAGGCAGGAAAA
GATTATCCCCTAA

Upstream 100 bases:

>100_bases
ACAATTGGTCAGCAGTTTGCTAGGGCATGCTGTAGTGCTAAGTGATAGCCTTAATCACTTTTTTAGTTTGTTGGATTGTT
TGTTTTTTCAAGGAGATTTT

Downstream 100 bases:

>100_bases
TCAACTGTGTTATGGGTGACAGATGAAAACAAAAAGCCTAGTGGGATAATCTAGGCTTTTTGTTATTAATGGGTAAACCC
TTAGCCCATTAACGATTGCA

Product: Beta-lactamase

Products: NA

Alternate protein names: Beta-lactamase PSE-2 [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MRLFVISAVLVMSSISASPVFAASSPQKEWQETRSLDASFTQHQAKGVVVLWNENKQQGFTNNLKRANQGFLPASTFKIP
NSLIALDLGMVKDEHQVFKWDGKNRDIAAWNRDHNLISAMKYSVVPIYQEFARQIGDARMGKMIAVFDYGNEDISGNLDS
FWLDGGIRISAKEQIDFLRRLYHNKLHVSERSQRIVKQAMLTEANSDYIIRAKTGYAVRAEPSIGWWVGWVELDDNVWFF
AMNMDMPSADGLALRQAITKEVLRQEKIIP

Sequences:

>Translated_270_residues
MRLFVISAVLVMSSISASPVFAASSPQKEWQETRSLDASFTQHQAKGVVVLWNENKQQGFTNNLKRANQGFLPASTFKIP
NSLIALDLGMVKDEHQVFKWDGKNRDIAAWNRDHNLISAMKYSVVPIYQEFARQIGDARMGKMIAVFDYGNEDISGNLDS
FWLDGGIRISAKEQIDFLRRLYHNKLHVSERSQRIVKQAMLTEANSDYIIRAKTGYAVRAEPSIGWWVGWVELDDNVWFF
AMNMDMPSADGLALRQAITKEVLRQEKIIP
>Mature_270_residues
MRLFVISAVLVMSSISASPVFAASSPQKEWQETRSLDASFTQHQAKGVVVLWNENKQQGFTNNLKRANQGFLPASTFKIP
NSLIALDLGMVKDEHQVFKWDGKNRDIAAWNRDHNLISAMKYSVVPIYQEFARQIGDARMGKMIAVFDYGNEDISGNLDS
FWLDGGIRISAKEQIDFLRRLYHNKLHVSERSQRIVKQAMLTEANSDYIIRAKTGYAVRAEPSIGWWVGWVELDDNVWFF
AMNMDMPSADGLALRQAITKEVLRQEKIIP

Specific function: Hydrolyzes both carbenicillin and oxacillin [H]

COG id: COG2602

COG function: function code V; Beta-lactamase class D

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-D beta-lactamase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR002137
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00905 Transpeptidase [H]

EC number: =3.5.2.6 [H]

Molecular weight: Translated: 30759; Mature: 30759

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS00337 BETA_LACTAMASE_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFVISAVLVMSSISASPVFAASSPQKEWQETRSLDASFTQHQAKGVVVLWNENKQQGF
CEEHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCC
TNNLKRANQGFLPASTFKIPNSLIALDLGMVKDEHQVFKWDGKNRDIAAWNRDHNLISAM
HHHHHHHCCCCCCCHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEECCCCHHHHHH
KYSVVPIYQEFARQIGDARMGKMIAVFDYGNEDISGNLDSFWLDGGIRISAKEQIDFLRR
HHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCCCEEEECCCEEEEHHHHHHHHHH
LYHNKLHVSERSQRIVKQAMLTEANSDYIIRAKTGYAVRAEPSIGWWVGWVELDDNVWFF
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCEEEEEEEECCCEEEE
AMNMDMPSADGLALRQAITKEVLRQEKIIP
EEECCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLFVISAVLVMSSISASPVFAASSPQKEWQETRSLDASFTQHQAKGVVVLWNENKQQGF
CEEHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCHHHHHCCCCEEEEECCCCCCCC
TNNLKRANQGFLPASTFKIPNSLIALDLGMVKDEHQVFKWDGKNRDIAAWNRDHNLISAM
HHHHHHHCCCCCCCHHHCCCCCEEEEECCCCCCCCEEEEECCCCCCEEEECCCCHHHHHH
KYSVVPIYQEFARQIGDARMGKMIAVFDYGNEDISGNLDSFWLDGGIRISAKEQIDFLRR
HHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCCCCCCEEEECCCEEEEHHHHHHHHHH
LYHNKLHVSERSQRIVKQAMLTEANSDYIIRAKTGYAVRAEPSIGWWVGWVELDDNVWFF
HHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCCEEEEEEEECCCEEEE
AMNMDMPSADGLALRQAITKEVLRQEKIIP
EEECCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3126705; 11188693 [H]