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The map label for this gene is pleD [H]

Identifier: 120597606

GI number: 120597606

Start: 865846

End: 866934

Strand: Direct

Name: pleD [H]

Synonym: Sputw3181_0775

Alternate gene names: 120597606

Gene position: 865846-866934 (Clockwise)

Preceding gene: 120597605

Following gene: 120597617

Centisome position: 18.39

GC content: 40.77

Gene sequence:

>1089_bases
ATGCAGAGCTTGATTACAACGACTGGAGCAAAAGATGACGAGCTTAGTGCCCATTCTGCTGCGGCATCCCAAGAAGCACT
GCAACTACTCAATGGTATTAGGCCATTTTCTATTACCATAAATGGCATTTTTGCCATCCTGTTTGTATTTTTATCCGTAT
GGCCTTTAGCATTTATTTATACCTTAGGGTTAAGCTATCACCTTTGGTTAAGCTACTGTGCTCACTCTCACCCTAAACAA
TTACCACCAATTATTCAGTGGTCTTGGGTGGTGGTATTTAGTCAATCTCTATCGTCAGTACTCATTTTAGGTGCTGATGC
TGGATTTCAATATTATCTTCTTGCGACTATACCACCATCATTTGCAAGTATTTTTAAGCCATTATTTTATAAACTAATAC
AAGCTTTGATCATCATAGTCTTCTTTCTTATTTGTGATATTTGGTTTATTGATTACGAGCCTTATTATCTTTATTCCGAT
GAAATGATTAGTTTATTTCGCCATATCAATGTGCTCGGTGCGTGTGCTTTGCTCGCCGGTGTATCCTATGCGTATTCACA
AATTGTAATGTCAGCCATTACTGCGCTACGTAATGTGGCTAATACAGATGAGTTAACAGGGCTGATTAACCGCCGGAGCT
TAACTGCACTTATTGATCGAGAAGCGGCAAGAACATTACGTACGCACGCATCGATGAGCTTAGTTTTATGTGATATTGAT
TATTTTAAACGTATTAATGATAGCTATGGTCATGCTGCTGGCGATCATGTGTTGGTTAATGTCGCTAATTTATTACAGGG
AGTGTTGAGGGAATATGATGTAGTTGCACGTTGGGGAGGAGAGGAGTTTTTATTGCTGTTACCCAATACGGATATAATAG
AAGCGATGAAAGTCGCTGAGCGATTACGCGAAATTGTTGCTTGTAGCCACCTCAGCTTTGAAGAGCAAACTATACCTGTG
ACGATGACACTCGGTGTTGCCGTTTTAGGGGATGAAAATTGGCACAGCACGTTAGCACGTGCTGATGAAGCGCTTTATCG
TGGTAAAAGACAAGGTCGAAATTGTGTTGTGCAGGCATCAGCCCCGTGA

Upstream 100 bases:

>100_bases
ATTGGTCAGTGAGGTTATTAACGAATATGGTTTCATCTGATTTGCCAAACACTTGCTACTATATAATGTAGAGGTAAATT
ACAATCAGTTGGGGGCTAGT

Downstream 100 bases:

>100_bases
CCACACGGGGCGGTGCAGATATTAGCTTTGCAGTATCTTAAGTGGCAGGCTAAGCATATCGTCACCTGTTCCAGCTAAGT
GCCAGATAATACCTACTAAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MQSLITTTGAKDDELSAHSAAASQEALQLLNGIRPFSITINGIFAILFVFLSVWPLAFIYTLGLSYHLWLSYCAHSHPKQ
LPPIIQWSWVVVFSQSLSSVLILGADAGFQYYLLATIPPSFASIFKPLFYKLIQALIIIVFFLICDIWFIDYEPYYLYSD
EMISLFRHINVLGACALLAGVSYAYSQIVMSAITALRNVANTDELTGLINRRSLTALIDREAARTLRTHASMSLVLCDID
YFKRINDSYGHAAGDHVLVNVANLLQGVLREYDVVARWGGEEFLLLLPNTDIIEAMKVAERLREIVACSHLSFEEQTIPV
TMTLGVAVLGDENWHSTLARADEALYRGKRQGRNCVVQASAP

Sequences:

>Translated_362_residues
MQSLITTTGAKDDELSAHSAAASQEALQLLNGIRPFSITINGIFAILFVFLSVWPLAFIYTLGLSYHLWLSYCAHSHPKQ
LPPIIQWSWVVVFSQSLSSVLILGADAGFQYYLLATIPPSFASIFKPLFYKLIQALIIIVFFLICDIWFIDYEPYYLYSD
EMISLFRHINVLGACALLAGVSYAYSQIVMSAITALRNVANTDELTGLINRRSLTALIDREAARTLRTHASMSLVLCDID
YFKRINDSYGHAAGDHVLVNVANLLQGVLREYDVVARWGGEEFLLLLPNTDIIEAMKVAERLREIVACSHLSFEEQTIPV
TMTLGVAVLGDENWHSTLARADEALYRGKRQGRNCVVQASAP
>Mature_362_residues
MQSLITTTGAKDDELSAHSAAASQEALQLLNGIRPFSITINGIFAILFVFLSVWPLAFIYTLGLSYHLWLSYCAHSHPKQ
LPPIIQWSWVVVFSQSLSSVLILGADAGFQYYLLATIPPSFASIFKPLFYKLIQALIIIVFFLICDIWFIDYEPYYLYSD
EMISLFRHINVLGACALLAGVSYAYSQIVMSAITALRNVANTDELTGLINRRSLTALIDREAARTLRTHASMSLVLCDID
YFKRINDSYGHAAGDHVLVNVANLLQGVLREYDVVARWGGEEFLLLLPNTDIIEAMKVAERLREIVACSHLSFEEQTIPV
TMTLGVAVLGDENWHSTLARADEALYRGKRQGRNCVVQASAP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=42.1686746987952, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=37.037037037037, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1787802, Length=200, Percent_Identity=31, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=36.4161849710983, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=40.251572327044, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787816, Length=158, Percent_Identity=37.9746835443038, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=37.5, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=32.7777777777778, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=33.1288343558282, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=37.1428571428571, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87081974, Length=155, Percent_Identity=27.0967741935484, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787056, Length=174, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=160, Percent_Identity=33.75, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40375; Mature: 40375

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSLITTTGAKDDELSAHSAAASQEALQLLNGIRPFSITINGIFAILFVFLSVWPLAFIY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
TLGLSYHLWLSYCAHSHPKQLPPIIQWSWVVVFSQSLSSVLILGADAGFQYYLLATIPPS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEEECCHH
FASIFKPLFYKLIQALIIIVFFLICDIWFIDYEPYYLYSDEMISLFRHINVLGACALLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VSYAYSQIVMSAITALRNVANTDELTGLINRRSLTALIDREAARTLRTHASMSLVLCDID
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHH
YFKRINDSYGHAAGDHVLVNVANLLQGVLREYDVVARWGGEEFLLLLPNTDIIEAMKVAE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
RLREIVACSHLSFEEQTIPVTMTLGVAVLGDENWHSTLARADEALYRGKRQGRNCVVQAS
HHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
AP
CC
>Mature Secondary Structure
MQSLITTTGAKDDELSAHSAAASQEALQLLNGIRPFSITINGIFAILFVFLSVWPLAFIY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
TLGLSYHLWLSYCAHSHPKQLPPIIQWSWVVVFSQSLSSVLILGADAGFQYYLLATIPPS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEEECCHH
FASIFKPLFYKLIQALIIIVFFLICDIWFIDYEPYYLYSDEMISLFRHINVLGACALLAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
VSYAYSQIVMSAITALRNVANTDELTGLINRRSLTALIDREAARTLRTHASMSLVLCDID
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECHH
YFKRINDSYGHAAGDHVLVNVANLLQGVLREYDVVARWGGEEFLLLLPNTDIIEAMKVAE
HHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
RLREIVACSHLSFEEQTIPVTMTLGVAVLGDENWHSTLARADEALYRGKRQGRNCVVQAS
HHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA