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The map label for this gene is btuB [H]

Identifier: 120597598

GI number: 120597598

Start: 852810

End: 854771

Strand: Direct

Name: btuB [H]

Synonym: Sputw3181_0767

Alternate gene names: 120597598

Gene position: 852810-854771 (Clockwise)

Preceding gene: 120597597

Following gene: 120597600

Centisome position: 18.11

GC content: 46.74

Gene sequence:

>1962_bases
ATGGGAACAAATCCAACTAAAATCGCGCTGCTGATCGGCAGCCTTTGCAGTTTTTCAATTATCGCGCCCGCTCTGGCGGC
CGACGATACCCATACCGCTAGAGTCGATGAGCATCTTGTCGTGATTGGCCGTACAGACAATACCCCGCTAAACATTGCTG
CCAATGTGAATGTGATTGATGCCGCTGCAATTGAAATGAGCGGTGCAACGAGTTTGACTGATGTACTACGCGGTCGAAGC
GGTATTCAAATTTCGGATAATAATATTGGGACTAGCTTTGCGATGCGCGGTTTTAGCGCCAGCACTGCTGTTAACAATAC
ACTGATTTTACTCGATGGCCGTCGTCTGAATAATATCGATATCGCTGCGCCAAGCCTCAATGCGATTCCGCTTAACCTTG
TTGAACGTATTGAGATTTTATCGGGCAGTGCGGGTGTGCTTTATGGCGACCAAGCTGTCGGTGGGGTGATTAACATTGTG
ACTAAGTCACCCGAAAACACTAGCGGCAGTGTGCAATTATCGGGTGGCAGCTTTGATACCTATGAAGGTCGCGGTGATAT
ATCTGGCGCCATCAATAAAGACTGGCGTTACTTTTTTACAGGTAGCTACAACCAAGGTGATAACTATCGCAAGCATAATG
CCAATGAAACTGGCTCAATTTTAGGCCGTATTCAATATAAAACTGATACAGATGCTTTTTTTGTTGAAACCAGTTATTAC
GATGATGACCGTGAAAATCCCGGTTCACTTACCAAAGATCAATTTAAACAAAACCCTCGTCAATCTTCCAATAAAAGCGA
ATATGTCCATGAGATGACCACAGCGGCGCGCAGTGGTTATGAGCATCAGCTTAACCCAAATTGGGCCTTAGCGGCGGATC
TGGATTATAGCGATACCTTAGTGACCTCGCTGAACTGGGGGGCAGGTAGTCATAATACCCGTTCTCTCTTAATGTTTAGT
CCTAAGGCCTTAGCAAATTACACCACTCGCCAAGGTGAGCTAAATCTAGTGGCAGGTTTAGATCTTAGCCGTGGTAAGGC
CGACTATAACACTATGGCGCGTAGCAATGTGCAAGAAATGCAAAGTGCTTATGTACAGGCCACAGTGCCATTAAGCCACA
GCTTAAGCTATGTGGTAGGTGGACGTTATGCGCGGGTGACCGACGAGTTAGTCGATGGCAATGTCTATCCAAATGGTATT
GATTTAGATGAAGATGCCACTGCATTCGAGTTCGGTCTAAACTATCGCCCAAGCGCCGAGCACCGATTCTATCTACGCGG
CGATGAAAACTTCCGTTTCGCTAAGGTCGATGAGCAAGCCTACACCCCGAAAGATGTCTATGGCCTAAAGCCGCAGACGG
GCCGCTCCTATGAGGCGGGTTGGGATTGGACTGTTGCTAGCCAAAGCCTGCGCGTAAGCTTATACCGCCTCGATTTAGAA
GATGAAATTGTCTTCGACCCCAGTGCGGAAAAACCTGTTGGTGGTAGTTTCCAAGGCGCGAACGTCAATGCCGATGCCTC
ACGCCGTTATGGTGCGAGCGCAGACTGGGATTGGCAGATCACTCAAGCACTGCAATTAGGGCTTGAATATAACTATATCG
ATGCCGAATTTACCGATGGGGTGAATGATGGTAAAGCACTGTCTTGGGTGGCAAAACATACTGGACGTGGTTATATCAGT
ATGGATTTCGCCGAGCATTTCCAAGTGTTTGCCGAGGCGATTTATACGGGTAATCGTTTTATTGAAGGCGATAATGCTAA
TGAAGGCGATAAGCTGGCAAGTTATGTGCTCGGTAATTTAGCCTTAAATTATAATCGTGATGCTTGGTTAGCGAGTCTAC
GCGTCGATAATTTGTTCGATAAAGAATATGTTAGCTCAGGTTATTATGGTGGTGGTTTGTGGGATAGTTATTATTCTGGC
CGAGGCCGTGATATCCGTCTAACCTTAGGTTATCGCTTCTAA

Upstream 100 bases:

>100_bases
GGGCAGATCTCAAAATGCGATAATCACCGTCAGTATTTACGCTTAATCAACGGTATCGTGGCGTCAATGCCCCGTTTTCC
TTGTAGGAGTTAAAGGTAAA

Downstream 100 bases:

>100_bases
TTACTTAGAAAATATATTTAGCCACCTTTAAATAATTTTTAAGGTGGCTTTTTTACGCTTAAATGATGCCTATTACTTAA
GTGCAGATTGATTGTCTATA

Product: TonB-dependent siderophore receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 653; Mature: 652

Protein sequence:

>653_residues
MGTNPTKIALLIGSLCSFSIIAPALAADDTHTARVDEHLVVIGRTDNTPLNIAANVNVIDAAAIEMSGATSLTDVLRGRS
GIQISDNNIGTSFAMRGFSASTAVNNTLILLDGRRLNNIDIAAPSLNAIPLNLVERIEILSGSAGVLYGDQAVGGVINIV
TKSPENTSGSVQLSGGSFDTYEGRGDISGAINKDWRYFFTGSYNQGDNYRKHNANETGSILGRIQYKTDTDAFFVETSYY
DDDRENPGSLTKDQFKQNPRQSSNKSEYVHEMTTAARSGYEHQLNPNWALAADLDYSDTLVTSLNWGAGSHNTRSLLMFS
PKALANYTTRQGELNLVAGLDLSRGKADYNTMARSNVQEMQSAYVQATVPLSHSLSYVVGGRYARVTDELVDGNVYPNGI
DLDEDATAFEFGLNYRPSAEHRFYLRGDENFRFAKVDEQAYTPKDVYGLKPQTGRSYEAGWDWTVASQSLRVSLYRLDLE
DEIVFDPSAEKPVGGSFQGANVNADASRRYGASADWDWQITQALQLGLEYNYIDAEFTDGVNDGKALSWVAKHTGRGYIS
MDFAEHFQVFAEAIYTGNRFIEGDNANEGDKLASYVLGNLALNYNRDAWLASLRVDNLFDKEYVSSGYYGGGLWDSYYSG
RGRDIRLTLGYRF

Sequences:

>Translated_653_residues
MGTNPTKIALLIGSLCSFSIIAPALAADDTHTARVDEHLVVIGRTDNTPLNIAANVNVIDAAAIEMSGATSLTDVLRGRS
GIQISDNNIGTSFAMRGFSASTAVNNTLILLDGRRLNNIDIAAPSLNAIPLNLVERIEILSGSAGVLYGDQAVGGVINIV
TKSPENTSGSVQLSGGSFDTYEGRGDISGAINKDWRYFFTGSYNQGDNYRKHNANETGSILGRIQYKTDTDAFFVETSYY
DDDRENPGSLTKDQFKQNPRQSSNKSEYVHEMTTAARSGYEHQLNPNWALAADLDYSDTLVTSLNWGAGSHNTRSLLMFS
PKALANYTTRQGELNLVAGLDLSRGKADYNTMARSNVQEMQSAYVQATVPLSHSLSYVVGGRYARVTDELVDGNVYPNGI
DLDEDATAFEFGLNYRPSAEHRFYLRGDENFRFAKVDEQAYTPKDVYGLKPQTGRSYEAGWDWTVASQSLRVSLYRLDLE
DEIVFDPSAEKPVGGSFQGANVNADASRRYGASADWDWQITQALQLGLEYNYIDAEFTDGVNDGKALSWVAKHTGRGYIS
MDFAEHFQVFAEAIYTGNRFIEGDNANEGDKLASYVLGNLALNYNRDAWLASLRVDNLFDKEYVSSGYYGGGLWDSYYSG
RGRDIRLTLGYRF
>Mature_652_residues
GTNPTKIALLIGSLCSFSIIAPALAADDTHTARVDEHLVVIGRTDNTPLNIAANVNVIDAAAIEMSGATSLTDVLRGRSG
IQISDNNIGTSFAMRGFSASTAVNNTLILLDGRRLNNIDIAAPSLNAIPLNLVERIEILSGSAGVLYGDQAVGGVINIVT
KSPENTSGSVQLSGGSFDTYEGRGDISGAINKDWRYFFTGSYNQGDNYRKHNANETGSILGRIQYKTDTDAFFVETSYYD
DDRENPGSLTKDQFKQNPRQSSNKSEYVHEMTTAARSGYEHQLNPNWALAADLDYSDTLVTSLNWGAGSHNTRSLLMFSP
KALANYTTRQGELNLVAGLDLSRGKADYNTMARSNVQEMQSAYVQATVPLSHSLSYVVGGRYARVTDELVDGNVYPNGID
LDEDATAFEFGLNYRPSAEHRFYLRGDENFRFAKVDEQAYTPKDVYGLKPQTGRSYEAGWDWTVASQSLRVSLYRLDLED
EIVFDPSAEKPVGGSFQGANVNADASRRYGASADWDWQITQALQLGLEYNYIDAEFTDGVNDGKALSWVAKHTGRGYISM
DFAEHFQVFAEAIYTGNRFIEGDNANEGDKLASYVLGNLALNYNRDAWLASLRVDNLFDKEYVSSGYYGGGLWDSYYSGR
GRDIRLTLGYRF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG1629

COG function: function code P; Outer membrane receptor proteins, mostly Fe transport

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787723, Length=701, Percent_Identity=25.2496433666191, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1788478, Length=182, Percent_Identity=34.0659340659341, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1790405, Length=232, Percent_Identity=29.7413793103448, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1790743, Length=290, Percent_Identity=23.1034482758621, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010916 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 71804; Mature: 71672

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTNPTKIALLIGSLCSFSIIAPALAADDTHTARVDEHLVVIGRTDNTPLNIAANVNVID
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCEEEEECCEEEE
AAAIEMSGATSLTDVLRGRSGIQISDNNIGTSFAMRGFSASTAVNNTLILLDGRRLNNID
EEEEEECCCCHHHHHHCCCCCCEEECCCCCCHHEECCCCCCCCCCCEEEEEECCCCCCEE
IAAPSLNAIPLNLVERIEILSGSAGVLYGDQAVGGVINIVTKSPENTSGSVQLSGGSFDT
EECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCEEEECCCCEEC
YEGRGDISGAINKDWRYFFTGSYNQGDNYRKHNANETGSILGRIQYKTDTDAFFVETSYY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEEC
DDDRENPGSLTKDQFKQNPRQSSNKSEYVHEMTTAARSGYEHQLNPNWALAADLDYSDTL
CCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEE
VTSLNWGAGSHNTRSLLMFSPKALANYTTRQGELNLVAGLDLSRGKADYNTMARSNVQEM
EEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCHHHH
QSAYVQATVPLSHSLSYVVGGRYARVTDELVDGNVYPNGIDLDEDATAFEFGLNYRPSAE
HHHHEEEEECHHCCCEEEECCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCC
HRFYLRGDENFRFAKVDEQAYTPKDVYGLKPQTGRSYEAGWDWTVASQSLRVSLYRLDLE
CEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCC
DEIVFDPSAEKPVGGSFQGANVNADASRRYGASADWDWQITQALQLGLEYNYIDAEFTDG
CCEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCC
VNDGKALSWVAKHTGRGYISMDFAEHFQVFAEAIYTGNRFIEGDNANEGDKLASYVLGNL
CCCCCCEEHHHHHCCCCEEEECHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHE
ALNYNRDAWLASLRVDNLFDKEYVSSGYYGGGLWDSYYSGRGRDIRLTLGYRF
EEECCCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCC
>Mature Secondary Structure 
GTNPTKIALLIGSLCSFSIIAPALAADDTHTARVDEHLVVIGRTDNTPLNIAANVNVID
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCEEEEECCEEEE
AAAIEMSGATSLTDVLRGRSGIQISDNNIGTSFAMRGFSASTAVNNTLILLDGRRLNNID
EEEEEECCCCHHHHHHCCCCCCEEECCCCCCHHEECCCCCCCCCCCEEEEEECCCCCCEE
IAAPSLNAIPLNLVERIEILSGSAGVLYGDQAVGGVINIVTKSPENTSGSVQLSGGSFDT
EECCCCCCCCHHHHHHHHHHCCCCCEEECCCCCCCEEEEEECCCCCCCCEEEECCCCEEC
YEGRGDISGAINKDWRYFFTGSYNQGDNYRKHNANETGSILGRIQYKTDTDAFFVETSYY
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEEEEC
DDDRENPGSLTKDQFKQNPRQSSNKSEYVHEMTTAARSGYEHQLNPNWALAADLDYSDTL
CCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCEE
VTSLNWGAGSHNTRSLLMFSPKALANYTTRQGELNLVAGLDLSRGKADYNTMARSNVQEM
EEEECCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHHCCHHHH
QSAYVQATVPLSHSLSYVVGGRYARVTDELVDGNVYPNGIDLDEDATAFEFGLNYRPSAE
HHHHEEEEECHHCCCEEEECCEEEEEHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCC
HRFYLRGDENFRFAKVDEQAYTPKDVYGLKPQTGRSYEAGWDWTVASQSLRVSLYRLDLE
CEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEEEECCC
DEIVFDPSAEKPVGGSFQGANVNADASRRYGASADWDWQITQALQLGLEYNYIDAEFTDG
CCEEECCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCC
VNDGKALSWVAKHTGRGYISMDFAEHFQVFAEAIYTGNRFIEGDNANEGDKLASYVLGNL
CCCCCCEEHHHHHCCCCEEEECHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHE
ALNYNRDAWLASLRVDNLFDKEYVSSGYYGGGLWDSYYSGRGRDIRLTLGYRF
EEECCCCCEEEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA