Click here to switch to the map view.

The map label for this gene is fliF

Identifier: 120597294

GI number: 120597294

Start: 507547

End: 509223

Strand: Direct

Name: fliF

Synonym: Sputw3181_0463

Alternate gene names: 120597294

Gene position: 507547-509223 (Clockwise)

Preceding gene: 120597293

Following gene: 120597295

Centisome position: 10.78

GC content: 45.8

Gene sequence:

>1677_bases
ATGTCTACCACTTTTGTTCAATCAGGTCAGGATATTACGGGAACTGAGCGTTCGTCAGGCCTCATTGCTGATATGACCCA
GAAATGGCATAAGTTTAACCGTGGAGATCGTCAGGTCATTGCCTTAGCGCTACTCGCTGTGGTTGTTGCCAGCGTAATAG
TGTTAATGCTCTGGACGGCAACGGCGGGGTATCGACCTTTATACGGTAGCCAAGAAAATGTCGATACCTCTCAAGTGCTT
AATGTATTAGAAGCTGAAGGGATTCATTACCGTCTTGAGGCAAATACAGGGGCAGTGCTGGTGGCTGAGGAGCAAGTCGG
TAATGCACGTATGCTCTTGGCGGCGAAAGGCGTGAAAGCGAAAGTACCTTCAGGTATGGAGGCGCTCGATAATAATGCGC
TTGGTACGAGCCAATTTATGGAGCAAGCCAAATATCGCCATAGTTTAGAAGGTGAGTTAGCCCGCACTATTATGTCATTA
AAGTTAGTGAGAGCGGCCAGAGTTCATTTAGCCATACCTAAACAGAGTCTATTTATTCGTCAGGAGCCGGAATTACCCAC
GGCCTCTGTGATGTTGCAGCTTGACCCCAATACACGTTTATCTGAATCACAGGTTGAGGCCATTGTTAACTTAGTGGCTG
GCAGTGTAACTGGACTCACCGCAAGTAATATTAAAGTCGTTGATCAAGATGGTCGGTATTTAAGTGAAAACATTTCGGGT
AATCAAGATCTTAGCCAGTCTAGGAATAAGCAATTACAATACACTCGTGAGTTGGAACATAGTTTAGTCGCCAATGCTGC
CTCTATGCTGGAGCCTGTGCTTGGACACGATAATTTCCAAGTACGAGTGACGGCAAAAGTTAACTTCAATCAGGTTGAAG
AAACCAAGGAGTCACTCGATCCACAAAATGTCGTAACCCAAGAGCGCACCAGTATTGATGACTCGAGTAATAGTATTGCG
GCAGGGATCCCTGGTGCTTTAAGCAATAAGCCTCCTCAGGTCGGGACCGCGGCAACGGATGACAAAACACGTAATCTGAA
GCAAGAGGAAAGTCGTCAATATGATGTTGGACGTTCTGTGCGTCATGTGCGTTACCAACAAATGCAGCTTGAAAACCTCT
CTGTCTCAGTACTCATCAATAGCGCGGCGGGTCAAGGGGTATTTAATAATGAGGCTCAGCTTGTTAAGTTTGGCAACATG
GTGAAAGATGCCATTGGTTTTTCAGCGGCAAGAGGTGACAGTTTTACCATCAATGCTTTTGAATTTACGCCGACAGTGGT
GGCTGAAATACCGACTTCTCCTTGGTGGCAATCCGAAAATTATCAATCCTATTTACGCTATATCATAGGTGGGATCCTCG
GTTTTGGGCTTATTTTGTTTGTCTTACGTCCTTTGGTTAAACACTTAACTCGTACGGCACAAATCACTGCCCCTAGTATA
GAACCAGCAGCATTAAGTGCGGCATCAGCCAATGCCCTTGATGGGCCAACGGTTGATTATGCACCTAATCAAGCCCATCA
ATTACCGAGTGCTGATTGGCTAGGCAGCCAAGGTTTGCCTGAACCAGGATCACCTTTAACGGTGAAGATGGAACATTTAG
CATTATTGGCAAATAAAGAACCCGCACGTGTTGCAGAAGTGATAGCTCATTGGATAGGCGATAAAGACGGTGAATAA

Upstream 100 bases:

>100_bases
AAAAGCCAGTTTGTCCTTTGCGACTATGCTTCAAATTCGTAATCGTCTTGTGCAAGCATTTGACGATGTAATGAAGATGC
CTATTTAAGGAATTGTGTGA

Downstream 100 bases:

>100_bases
CTCAATCGAAATTAAGATGGATAATTACGCCCAAGCAGCCATGTTATTGCTGAGTATGGGAGAAGAAGGTGCAGCTAGGG
TGATGGCGCATTTAGACCGT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MSTTFVQSGQDITGTERSSGLIADMTQKWHKFNRGDRQVIALALLAVVVASVIVLMLWTATAGYRPLYGSQENVDTSQVL
NVLEAEGIHYRLEANTGAVLVAEEQVGNARMLLAAKGVKAKVPSGMEALDNNALGTSQFMEQAKYRHSLEGELARTIMSL
KLVRAARVHLAIPKQSLFIRQEPELPTASVMLQLDPNTRLSESQVEAIVNLVAGSVTGLTASNIKVVDQDGRYLSENISG
NQDLSQSRNKQLQYTRELEHSLVANAASMLEPVLGHDNFQVRVTAKVNFNQVEETKESLDPQNVVTQERTSIDDSSNSIA
AGIPGALSNKPPQVGTAATDDKTRNLKQEESRQYDVGRSVRHVRYQQMQLENLSVSVLINSAAGQGVFNNEAQLVKFGNM
VKDAIGFSAARGDSFTINAFEFTPTVVAEIPTSPWWQSENYQSYLRYIIGGILGFGLILFVLRPLVKHLTRTAQITAPSI
EPAALSAASANALDGPTVDYAPNQAHQLPSADWLGSQGLPEPGSPLTVKMEHLALLANKEPARVAEVIAHWIGDKDGE

Sequences:

>Translated_558_residues
MSTTFVQSGQDITGTERSSGLIADMTQKWHKFNRGDRQVIALALLAVVVASVIVLMLWTATAGYRPLYGSQENVDTSQVL
NVLEAEGIHYRLEANTGAVLVAEEQVGNARMLLAAKGVKAKVPSGMEALDNNALGTSQFMEQAKYRHSLEGELARTIMSL
KLVRAARVHLAIPKQSLFIRQEPELPTASVMLQLDPNTRLSESQVEAIVNLVAGSVTGLTASNIKVVDQDGRYLSENISG
NQDLSQSRNKQLQYTRELEHSLVANAASMLEPVLGHDNFQVRVTAKVNFNQVEETKESLDPQNVVTQERTSIDDSSNSIA
AGIPGALSNKPPQVGTAATDDKTRNLKQEESRQYDVGRSVRHVRYQQMQLENLSVSVLINSAAGQGVFNNEAQLVKFGNM
VKDAIGFSAARGDSFTINAFEFTPTVVAEIPTSPWWQSENYQSYLRYIIGGILGFGLILFVLRPLVKHLTRTAQITAPSI
EPAALSAASANALDGPTVDYAPNQAHQLPSADWLGSQGLPEPGSPLTVKMEHLALLANKEPARVAEVIAHWIGDKDGE
>Mature_557_residues
STTFVQSGQDITGTERSSGLIADMTQKWHKFNRGDRQVIALALLAVVVASVIVLMLWTATAGYRPLYGSQENVDTSQVLN
VLEAEGIHYRLEANTGAVLVAEEQVGNARMLLAAKGVKAKVPSGMEALDNNALGTSQFMEQAKYRHSLEGELARTIMSLK
LVRAARVHLAIPKQSLFIRQEPELPTASVMLQLDPNTRLSESQVEAIVNLVAGSVTGLTASNIKVVDQDGRYLSENISGN
QDLSQSRNKQLQYTRELEHSLVANAASMLEPVLGHDNFQVRVTAKVNFNQVEETKESLDPQNVVTQERTSIDDSSNSIAA
GIPGALSNKPPQVGTAATDDKTRNLKQEESRQYDVGRSVRHVRYQQMQLENLSVSVLINSAAGQGVFNNEAQLVKFGNMV
KDAIGFSAARGDSFTINAFEFTPTVVAEIPTSPWWQSENYQSYLRYIIGGILGFGLILFVLRPLVKHLTRTAQITAPSIE
PAALSAASANALDGPTVDYAPNQAHQLPSADWLGSQGLPEPGSPLTVKMEHLALLANKEPARVAEVIAHWIGDKDGE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=443, Percent_Identity=30.0225733634311, Blast_Score=219, Evalue=5e-58,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60630; Mature: 60499

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTFVQSGQDITGTERSSGLIADMTQKWHKFNRGDRQVIALALLAVVVASVIVLMLWTA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGYRPLYGSQENVDTSQVLNVLEAEGIHYRLEANTGAVLVAEEQVGNARMLLAAKGVKA
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCCC
KVPSGMEALDNNALGTSQFMEQAKYRHSLEGELARTIMSLKLVRAARVHLAIPKQSLFIR
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHEEEE
QEPELPTASVMLQLDPNTRLSESQVEAIVNLVAGSVTGLTASNIKVVDQDGRYLSENISG
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCHHHCCCC
NQDLSQSRNKQLQYTRELEHSLVANAASMLEPVLGHDNFQVRVTAKVNFNQVEETKESLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHCC
PQNVVTQERTSIDDSSNSIAAGIPGALSNKPPQVGTAATDDKTRNLKQEESRQYDVGRSV
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
RHVRYQQMQLENLSVSVLINSAAGQGVFNNEAQLVKFGNMVKDAIGFSAARGDSFTINAF
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EFTPTVVAEIPTSPWWQSENYQSYLRYIIGGILGFGLILFVLRPLVKHLTRTAQITAPSI
EECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EPAALSAASANALDGPTVDYAPNQAHQLPSADWLGSQGLPEPGSPLTVKMEHLALLANKE
CHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCC
PARVAEVIAHWIGDKDGE
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STTFVQSGQDITGTERSSGLIADMTQKWHKFNRGDRQVIALALLAVVVASVIVLMLWTA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TAGYRPLYGSQENVDTSQVLNVLEAEGIHYRLEANTGAVLVAEEQVGNARMLLAAKGVKA
CCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEEEECCCCCCEEEEEEECCCCC
KVPSGMEALDNNALGTSQFMEQAKYRHSLEGELARTIMSLKLVRAARVHLAIPKQSLFIR
CCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHEEEE
QEPELPTASVMLQLDPNTRLSESQVEAIVNLVAGSVTGLTASNIKVVDQDGRYLSENISG
CCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCEEEEECCCCCHHHCCCC
NQDLSQSRNKQLQYTRELEHSLVANAASMLEPVLGHDNFQVRVTAKVNFNQVEETKESLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECHHHHHHHHHHCC
PQNVVTQERTSIDDSSNSIAAGIPGALSNKPPQVGTAATDDKTRNLKQEESRQYDVGRSV
HHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHH
RHVRYQQMQLENLSVSVLINSAAGQGVFNNEAQLVKFGNMVKDAIGFSAARGDSFTINAF
HHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EFTPTVVAEIPTSPWWQSENYQSYLRYIIGGILGFGLILFVLRPLVKHLTRTAQITAPSI
EECCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
EPAALSAASANALDGPTVDYAPNQAHQLPSADWLGSQGLPEPGSPLTVKMEHLALLANKE
CHHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCC
PARVAEVIAHWIGDKDGE
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]