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The map label for this gene is flbD [H]

Identifier: 120597292

GI number: 120597292

Start: 505859

End: 507169

Strand: Direct

Name: flbD [H]

Synonym: Sputw3181_0461

Alternate gene names: 120597292

Gene position: 505859-507169 (Clockwise)

Preceding gene: 120597278

Following gene: 120597293

Centisome position: 10.74

GC content: 45.61

Gene sequence:

>1311_bases
GTGAGAAGCCTAAGTATACGGATTGAAGGGCTAAATCTATCGGCGGATCTTATATGCAAAATAATGCATGAAAATTTTTG
CCTTTGGAATGATAAAATGGACTTGCCTTGGCTCACAATCGTCAATCTTTCTGATTGTAGTCCTAGTGACGTAAAGGATT
ATCTCGCTCGTTTCCCTAGTCGTAACCAGCTTGCTTTGTTGGCGCCAGAACAAGGTGAGTTAGCCGCCGCAGCGATGCGT
GCTGGCGTTCAAGACTATTTATTAATTCCCGTAGAAAGTGAGCAATTGCTCGCGTCTATTCATCGATTACGACGGTTAGA
GTTACCTGACTCTAGTTTGGTGGTATCGGCATCTGTCAGTCGGCAGTTGTTGATGCTCGCCCATCGCGCAGCAACCACTG
AAGCCAGCGTTCTATTACTGGGCGAAAGTGGTACAGGCAAAGAACCTCTCGCTCGTTATATTCACCGTCATTCTTCGCGT
TCACATAAACCTTTTATTGCGATTAATTGTGCTGCAATTCCAGAAAGTATCCTTGAGTCCGTATTGTTTGGGCACGTAAA
AGGCGCCTTTACAGGTGCAATTTGTGATAAGGCCGGGAAATTTGAGCAAGCCAATGGCGGTACGTTATTGCTCGATGAAA
TTGGTGAAATGCCTTTGACGTTGCAGGCGAAATTACTGCGAGTTTTGCAGGAAAGAGAAGTGGAAAGGCTGGGTGGGCAG
CATGCAATAGCGTTAGATATCAGGATAATTGCGTCTACTAATCGTGACTTAAGGCAGGCAGTAGAGCTCGGCCATTTCAG
AGAAGACTTATTTTATCGTTTAGATGTTTTACCGTTGAAAATTTCTCCTTTAAGAGATCGAAAGGCAGATATTTTGCCTC
TTGCTGAGCACTTCTTGGACTTATATCACCAGCCAGATACCACCAGTACTTGTTATTTTAGTGAGCACGCAAAGCAAGCC
CTTGTGTCCTATGATTGGCCCGGAAACGTAAGGGAATTAGAAAATTGTATCCAGCGGGCATTGGTGATGCGTCGTGGGCT
TGCAATACAAGCTGCCGATCTTGGCTTAAACATTCAGCTAGAAGTGCAGGCGGTTGAGCATCCAGAAGTGACTGATGGAC
TTAGGGCTTCAAAGCAGCAGGCGGAGTTCCAATATATTATCGACGTACTTAAGCGTTTCAATGGGCAACGTACCTTAAGT
GCACAGGCGTTAGGGATGACGACGAGAGCGTTAAGATATCGACTCGTACAAATGCGGGAAGCAGGGATTGATATTGAAAT
GTTATTAGAACAAGCGGGCCGCGCTGCTTAG

Upstream 100 bases:

>100_bases
GGTTAGTTGTAATAAATTTTATTTAAACTCAATTACACAAAAAATTAGTCCATTAACTAAGGTGTTATTTCATGTGCGGT
AAACAAGAGGGTGTTGGCGA

Downstream 100 bases:

>100_bases
CGTGATACAAAGTAATAGGAAAAAATATGTCTTCTGGAGCGATAGTTAGCTCGAATATGCTAATGGACACCTTAAGTATT
CATCGGGAAGTGGCTAAAGG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MRSLSIRIEGLNLSADLICKIMHENFCLWNDKMDLPWLTIVNLSDCSPSDVKDYLARFPSRNQLALLAPEQGELAAAAMR
AGVQDYLLIPVESEQLLASIHRLRRLELPDSSLVVSASVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSR
SHKPFIAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLTLQAKLLRVLQEREVERLGGQ
HAIALDIRIIASTNRDLRQAVELGHFREDLFYRLDVLPLKISPLRDRKADILPLAEHFLDLYHQPDTTSTCYFSEHAKQA
LVSYDWPGNVRELENCIQRALVMRRGLAIQAADLGLNIQLEVQAVEHPEVTDGLRASKQQAEFQYIIDVLKRFNGQRTLS
AQALGMTTRALRYRLVQMREAGIDIEMLLEQAGRAA

Sequences:

>Translated_436_residues
MRSLSIRIEGLNLSADLICKIMHENFCLWNDKMDLPWLTIVNLSDCSPSDVKDYLARFPSRNQLALLAPEQGELAAAAMR
AGVQDYLLIPVESEQLLASIHRLRRLELPDSSLVVSASVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSR
SHKPFIAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLTLQAKLLRVLQEREVERLGGQ
HAIALDIRIIASTNRDLRQAVELGHFREDLFYRLDVLPLKISPLRDRKADILPLAEHFLDLYHQPDTTSTCYFSEHAKQA
LVSYDWPGNVRELENCIQRALVMRRGLAIQAADLGLNIQLEVQAVEHPEVTDGLRASKQQAEFQYIIDVLKRFNGQRTLS
AQALGMTTRALRYRLVQMREAGIDIEMLLEQAGRAA
>Mature_436_residues
MRSLSIRIEGLNLSADLICKIMHENFCLWNDKMDLPWLTIVNLSDCSPSDVKDYLARFPSRNQLALLAPEQGELAAAAMR
AGVQDYLLIPVESEQLLASIHRLRRLELPDSSLVVSASVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSR
SHKPFIAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLTLQAKLLRVLQEREVERLGGQ
HAIALDIRIIASTNRDLRQAVELGHFREDLFYRLDVLPLKISPLRDRKADILPLAEHFLDLYHQPDTTSTCYFSEHAKQA
LVSYDWPGNVRELENCIQRALVMRRGLAIQAADLGLNIQLEVQAVEHPEVTDGLRASKQQAEFQYIIDVLKRFNGQRTLS
AQALGMTTRALRYRLVQMREAGIDIEMLLEQAGRAA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=372, Percent_Identity=45.4301075268817, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=45.3257790368272, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1790437, Length=380, Percent_Identity=39.4736842105263, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI1788905, Length=352, Percent_Identity=40.625, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI87082152, Length=241, Percent_Identity=50.207468879668, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=44.2006269592476, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1790299, Length=292, Percent_Identity=42.8082191780822, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=42.5867507886435, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1786524, Length=309, Percent_Identity=42.7184466019417, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=36.9426751592357, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI87081872, Length=217, Percent_Identity=44.7004608294931, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=36.6812227074236, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=31.0457516339869, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48708; Mature: 48708

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLSIRIEGLNLSADLICKIMHENFCLWNDKMDLPWLTIVNLSDCSPSDVKDYLARFPS
CCCEEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
RNQLALLAPEQGELAAAAMRAGVQDYLLIPVESEQLLASIHRLRRLELPDSSLVVSASVS
CCCEEEECCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEHHHH
RQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSRSHKPFIAINCAAIPESILES
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHH
VLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLTLQAKLLRVLQEREVERLGGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
HAIALDIRIIASTNRDLRQAVELGHFREDLFYRLDVLPLKISPLRDRKADILPLAEHFLD
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHH
LYHQPDTTSTCYFSEHAKQALVSYDWPGNVRELENCIQRALVMRRGLAIQAADLGLNIQL
HHCCCCCCCEEEEHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
EVQAVEHPEVTDGLRASKQQAEFQYIIDVLKRFNGQRTLSAQALGMTTRALRYRLVQMRE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AGIDIEMLLEQAGRAA
CCCCHHHHHHHCCCCC
>Mature Secondary Structure
MRSLSIRIEGLNLSADLICKIMHENFCLWNDKMDLPWLTIVNLSDCSPSDVKDYLARFPS
CCCEEEEEEECCCCHHHHHHHHHCCEEEECCCCCCCEEEEEEECCCCCHHHHHHHHHCCC
RNQLALLAPEQGELAAAAMRAGVQDYLLIPVESEQLLASIHRLRRLELPDSSLVVSASVS
CCCEEEECCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCCEEEEHHHH
RQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSRSHKPFIAINCAAIPESILES
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHH
VLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLTLQAKLLRVLQEREVERLGGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
HAIALDIRIIASTNRDLRQAVELGHFREDLFYRLDVLPLKISPLRDRKADILPLAEHFLD
EEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCHHHHHHHHHH
LYHQPDTTSTCYFSEHAKQALVSYDWPGNVRELENCIQRALVMRRGLAIQAADLGLNIQL
HHCCCCCCCEEEEHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEE
EVQAVEHPEVTDGLRASKQQAEFQYIIDVLKRFNGQRTLSAQALGMTTRALRYRLVQMRE
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AGIDIEMLLEQAGRAA
CCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]