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The map label for this gene is aaeR [H]

Identifier: 120597235

GI number: 120597235

Start: 445952

End: 446980

Strand: Direct

Name: aaeR [H]

Synonym: Sputw3181_0404

Alternate gene names: 120597235

Gene position: 445952-446980 (Clockwise)

Preceding gene: 120597233

Following gene: 120597236

Centisome position: 9.47

GC content: 42.27

Gene sequence:

>1029_bases
ATGTTACAATTGACTCAAGTTAGAATTTGGGTAATCACTTTTAAGAGTTACTTAAGAATGAATAAAAACATGATCAAGGG
GATGATCGTTTTTGCGACTATTGCGGAAAAGGGATCGATGAGTGCCGCTGCGCAGCAATTAAATCTCAGCTCGTCTGCGG
TAAGTCAGCAAGTGACCAAGCTCGAAATGGATATGGGGATAAGTCTATTTCACCGAAATACCCGCAACCTCACGCTCACC
GAGGCAGGCAGTTTGTTCTATGAGAATTGCCGCAAAGTGATCAGTATCGTTGATAGCACAGAGCAAAAACTTAATAACTT
AAAAGGTATTCCTTCTGGAGAATTGAAGATTGCGGCGCCTGTAGGATTTGGTGGTGGTTTATTAAGTGAACCTTTTCAGT
ATTTACTTGCAACTCATCCACAAATAAGTTTGAACCTCGTTATGCAGGATGGGCCCATAGATATAGTCAACGACGGTATC
GATCTGGCGATTTGCATTGGTCCACTCTCAGACTCAAACTTAATTGCCCGTCACTTAGCCGATTGGCGTATGTTGCTCTG
TACCTCACCTGATTACTTGCCTATGCATCATAATATCAAGCATCCATCTGAATTAGCGGCATTTAATCGAATCAATCACA
ATTACACTAAAGGCGAACTTTTAACCCATCATCCTAGTCTTGAGTGTTTTGAGCTCACGGGGCAGCGGATCAGCGTTAAC
AATATGCAGACCTTAATCCAGCTTACGCTTGATGGGTTAGGATATGCCGCCTTACCAGAACCTGAAGTGCTTGATTATCT
TGATAAAGGTGTATTAGTCCCCTTGTTACCCGAATGGTCATTCAATACTTACAGTGTTTACAGTGTGACATCTGCACGGG
ATAGGCAGCCTGCTAAAGTCAAAGAAGCCTTAAACGCGCTCAGCCAATATTTTAGTCAAACTGATTTTAACCATGCCGAT
CAATTGAAAGGTGGGAGTTATCAAACAGAGCATATTAAAAGTGGTAAAGAGCACAGGTTAGGCACTTAG

Upstream 100 bases:

>100_bases
TTGGGCGAGATACTTCTTACGACTCAACAACATAACCAACAAGTCTCCATAGAGAACATTTTGCAAATGAGAACAATTTT
CAATACGGAAATGTATTCCT

Downstream 100 bases:

>100_bases
CCTACTCGTTATGATGAGAGTGTAAGTTTTGTCACTATGTTGCCAGCGAAGGTGTAGAGTTTGCACATTCATTTCTTACT
CAAGCTGGGGTATTCTGCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MLQLTQVRIWVITFKSYLRMNKNMIKGMIVFATIAEKGSMSAAAQQLNLSSSAVSQQVTKLEMDMGISLFHRNTRNLTLT
EAGSLFYENCRKVISIVDSTEQKLNNLKGIPSGELKIAAPVGFGGGLLSEPFQYLLATHPQISLNLVMQDGPIDIVNDGI
DLAICIGPLSDSNLIARHLADWRMLLCTSPDYLPMHHNIKHPSELAAFNRINHNYTKGELLTHHPSLECFELTGQRISVN
NMQTLIQLTLDGLGYAALPEPEVLDYLDKGVLVPLLPEWSFNTYSVYSVTSARDRQPAKVKEALNALSQYFSQTDFNHAD
QLKGGSYQTEHIKSGKEHRLGT

Sequences:

>Translated_342_residues
MLQLTQVRIWVITFKSYLRMNKNMIKGMIVFATIAEKGSMSAAAQQLNLSSSAVSQQVTKLEMDMGISLFHRNTRNLTLT
EAGSLFYENCRKVISIVDSTEQKLNNLKGIPSGELKIAAPVGFGGGLLSEPFQYLLATHPQISLNLVMQDGPIDIVNDGI
DLAICIGPLSDSNLIARHLADWRMLLCTSPDYLPMHHNIKHPSELAAFNRINHNYTKGELLTHHPSLECFELTGQRISVN
NMQTLIQLTLDGLGYAALPEPEVLDYLDKGVLVPLLPEWSFNTYSVYSVTSARDRQPAKVKEALNALSQYFSQTDFNHAD
QLKGGSYQTEHIKSGKEHRLGT
>Mature_342_residues
MLQLTQVRIWVITFKSYLRMNKNMIKGMIVFATIAEKGSMSAAAQQLNLSSSAVSQQVTKLEMDMGISLFHRNTRNLTLT
EAGSLFYENCRKVISIVDSTEQKLNNLKGIPSGELKIAAPVGFGGGLLSEPFQYLLATHPQISLNLVMQDGPIDIVNDGI
DLAICIGPLSDSNLIARHLADWRMLLCTSPDYLPMHHNIKHPSELAAFNRINHNYTKGELLTHHPSLECFELTGQRISVN
NMQTLIQLTLDGLGYAALPEPEVLDYLDKGVLVPLLPEWSFNTYSVYSVTSARDRQPAKVKEALNALSQYFSQTDFNHAD
QLKGGSYQTEHIKSGKEHRLGT

Specific function: Activates transcription of the aaeXAB operon [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=31.5068493150685, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=30.5369127516779, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI145693193, Length=280, Percent_Identity=29.6428571428571, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786401, Length=310, Percent_Identity=29.3548387096774, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=27.7966101694915, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787128, Length=264, Percent_Identity=26.8939393939394, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787589, Length=268, Percent_Identity=26.1194029850746, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788706, Length=178, Percent_Identity=29.2134831460674, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI157672245, Length=149, Percent_Identity=28.1879194630872, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1790783, Length=266, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=129, Percent_Identity=24.031007751938, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789173, Length=213, Percent_Identity=26.7605633802817, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1788297, Length=235, Percent_Identity=23.4042553191489, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=24.9056603773585, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=129, Percent_Identity=28.6821705426357, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1788748, Length=183, Percent_Identity=29.5081967213115, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 38058; Mature: 38058

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLTQVRIWVITFKSYLRMNKNMIKGMIVFATIAEKGSMSAAAQQLNLSSSAVSQQVTK
CCEEEEEEEEEEEHHHHHHHCHHHHHHEEEEEEHHCCCCHHHHHHHHCCCHHHHHHHHHH
LEMDMGISLFHRNTRNLTLTEAGSLFYENCRKVISIVDSTEQKLNNLKGIPSGELKIAAP
HHHHCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
VGFGGGLLSEPFQYLLATHPQISLNLVMQDGPIDIVNDGIDLAICIGPLSDSNLIARHLA
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCEEEEEEECCCCCHHHHHHHH
DWRMLLCTSPDYLPMHHNIKHPSELAAFNRINHNYTKGELLTHHPSLECFELTGQRISVN
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCEEEHH
NMQTLIQLTLDGLGYAALPEPEVLDYLDKGVLVPLLPEWSFNTYSVYSVTSARDRQPAKV
HHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHH
KEALNALSQYFSQTDFNHADQLKGGSYQTEHIKSGKEHRLGT
HHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MLQLTQVRIWVITFKSYLRMNKNMIKGMIVFATIAEKGSMSAAAQQLNLSSSAVSQQVTK
CCEEEEEEEEEEEHHHHHHHCHHHHHHEEEEEEHHCCCCHHHHHHHHCCCHHHHHHHHHH
LEMDMGISLFHRNTRNLTLTEAGSLFYENCRKVISIVDSTEQKLNNLKGIPSGELKIAAP
HHHHCCEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
VGFGGGLLSEPFQYLLATHPQISLNLVMQDGPIDIVNDGIDLAICIGPLSDSNLIARHLA
CCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCEEEEEEECCCCCHHHHHHHH
DWRMLLCTSPDYLPMHHNIKHPSELAAFNRINHNYTKGELLTHHPSLECFELTGQRISVN
HHEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEECCCCCEEEEECCCEEEHH
NMQTLIQLTLDGLGYAALPEPEVLDYLDKGVLVPLLPEWSFNTYSVYSVTSARDRQPAKV
HHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCEEEEEECCCCCCCCHHH
KEALNALSQYFSQTDFNHADQLKGGSYQTEHIKSGKEHRLGT
HHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]