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The map label for this gene is hemN [H]

Identifier: 120597231

GI number: 120597231

Start: 441158

End: 442495

Strand: Direct

Name: hemN [H]

Synonym: Sputw3181_0400

Alternate gene names: 120597231

Gene position: 441158-442495 (Clockwise)

Preceding gene: 120597229

Following gene: 120597232

Centisome position: 9.37

GC content: 44.17

Gene sequence:

>1338_bases
ATGTCATCAGTTATTCAAACGCTCAACGGCGCTATCGCAACGCCTTATCAAGCGAATATTACCGTCCCTAACTGGATGCT
CGGCTCGATGGAACGGGTCATGCAGTACTATGTCGATAAGAATCTTCGCTTAGATACCTTGTCGGCAGAGATGATGCCTG
CACCTGTTGAGGGCAAAAAATACATGTTGTATGCTCATGTTCCATTTTGCCATACGCTTTGTTCCTACTGCACCTTCCAC
CGTTTTATGTTCAAGGAAGACAAGGCGCGTGCTTACTTCATCTCATTACGGAAAGAGATGGAAATGGTCAAATCCCTCGG
CTATGACTTTGAATCTATGTACATTGGTGGCGGAACCACCACAGTATTAGAAGACGAATTAGCCCGCACTATAGAACACG
CTAAAACCCTATTTCCTAGCATTAAAGAAGTCTCATGTGAGTCCGATCCGCAGCATTTAGACAGCCCAGGCTTTAAGCAA
CTCAAAGGCTTAGTGGATCGCATGTCTATCGGCGTGCAAAGCTTTAATGACGATATTTTAAAAATGACCGACCGACTTGA
TAAATTCGGTACAGGTCAGCAAACCTTCGACAAGATCATGGGCGCCAAAGAGTTGTTCCCTATTATCAATGTCGACCTGA
TTTTTGGTTTCCGCGGCCAAACTGATGAAGTGATCCAACACGATCTCGACATGGCATCGCGCTTAGATCCAAGACAAATT
ACCACCTATCCGCTGATGATCACTCATCAAACCCGTAAGAGCGTTAAAGGCAAATTAGCGGCGCCGCAAGCGGATATGGC
AAACCAATATCGCCAGATCTTAAACAGCCTCAATGGTCAATATAATCAGTTATCTGCATGGGCATTTGGTAAAGCGAATG
ACGAAGGTTTTGATGAGTATGTGATCGACTACGATGAGTATTTAGGGGTCGGCTCAGGTTCATTTAGCTTTTTGAATGAC
ACGCTTTATGTGAACACTTTCTCACTACGTAAGTACCAAGAGCGTATTGCAGCGGGTCAAATGGGTGTCGAGCAACAAAA
GAACTACAGTAAAAAAGACGTGATGCAATATCGCTTTTTGCTGGGCATGTTCTCAGGCCGTTTATCGCGTAAATATTTCC
GCGAAACCTTTGGCGTCAATTTAGATACCGCATTATTTAAAGAAATGACGTCAATGAAACTCATTGGTGCGATTAAAAAT
GACCCAACGGATCCCGATAATCTGATCGTAACCGATAACGGCAAGATGATGGGGCTATTGATGATGAAAGAGTTCTACGC
AGGTATGGATAACGTACGCGCTCAATTGCGTAAACCACTCAAACCTTGCGATATGTAA

Upstream 100 bases:

>100_bases
TTATCTTTTTCATACAGGGTAGTCTAATTGCGCTAGGTGGTCGTGAATTACCTAAAGCTATTTCGGTGTTTACGGTTAGC
ACTCTCAGATAGGAATCGTT

Downstream 100 bases:

>100_bases
GCACAGTTAACCACTAAAGGCCCTTTATGGGCCTTTTTGTTAGTGTGATTTCAGTTTATTCTTGCCACAACTCTCGCATG
AGCCACTTACCCAGTAATAA

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSSVIQTLNGAIATPYQANITVPNWMLGSMERVMQYYVDKNLRLDTLSAEMMPAPVEGKKYMLYAHVPFCHTLCSYCTFH
RFMFKEDKARAYFISLRKEMEMVKSLGYDFESMYIGGGTTTVLEDELARTIEHAKTLFPSIKEVSCESDPQHLDSPGFKQ
LKGLVDRMSIGVQSFNDDILKMTDRLDKFGTGQQTFDKIMGAKELFPIINVDLIFGFRGQTDEVIQHDLDMASRLDPRQI
TTYPLMITHQTRKSVKGKLAAPQADMANQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDYDEYLGVGSGSFSFLND
TLYVNTFSLRKYQERIAAGQMGVEQQKNYSKKDVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKLIGAIKN
DPTDPDNLIVTDNGKMMGLLMMKEFYAGMDNVRAQLRKPLKPCDM

Sequences:

>Translated_445_residues
MSSVIQTLNGAIATPYQANITVPNWMLGSMERVMQYYVDKNLRLDTLSAEMMPAPVEGKKYMLYAHVPFCHTLCSYCTFH
RFMFKEDKARAYFISLRKEMEMVKSLGYDFESMYIGGGTTTVLEDELARTIEHAKTLFPSIKEVSCESDPQHLDSPGFKQ
LKGLVDRMSIGVQSFNDDILKMTDRLDKFGTGQQTFDKIMGAKELFPIINVDLIFGFRGQTDEVIQHDLDMASRLDPRQI
TTYPLMITHQTRKSVKGKLAAPQADMANQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDYDEYLGVGSGSFSFLND
TLYVNTFSLRKYQERIAAGQMGVEQQKNYSKKDVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKLIGAIKN
DPTDPDNLIVTDNGKMMGLLMMKEFYAGMDNVRAQLRKPLKPCDM
>Mature_444_residues
SSVIQTLNGAIATPYQANITVPNWMLGSMERVMQYYVDKNLRLDTLSAEMMPAPVEGKKYMLYAHVPFCHTLCSYCTFHR
FMFKEDKARAYFISLRKEMEMVKSLGYDFESMYIGGGTTTVLEDELARTIEHAKTLFPSIKEVSCESDPQHLDSPGFKQL
KGLVDRMSIGVQSFNDDILKMTDRLDKFGTGQQTFDKIMGAKELFPIINVDLIFGFRGQTDEVIQHDLDMASRLDPRQIT
TYPLMITHQTRKSVKGKLAAPQADMANQYRQILNSLNGQYNQLSAWAFGKANDEGFDEYVIDYDEYLGVGSGSFSFLNDT
LYVNTFSLRKYQERIAAGQMGVEQQKNYSKKDVMQYRFLLGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKLIGAIKND
PTDPDNLIVTDNGKMMGLLMMKEFYAGMDNVRAQLRKPLKPCDM

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=286, Percent_Identity=26.5734265734266, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI87082341, Length=300, Percent_Identity=25.6666666666667, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789325, Length=266, Percent_Identity=25.5639097744361, Blast_Score=75, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 50840; Mature: 50709

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVIQTLNGAIATPYQANITVPNWMLGSMERVMQYYVDKNLRLDTLSAEMMPAPVEGKK
CCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCCE
YMLYAHVPFCHTLCSYCTFHRFMFKEDKARAYFISLRKEMEMVKSLGYDFESMYIGGGTT
EEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCH
TVLEDELARTIEHAKTLFPSIKEVSCESDPQHLDSPGFKQLKGLVDRMSIGVQSFNDDIL
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
KMTDRLDKFGTGQQTFDKIMGAKELFPIINVDLIFGFRGQTDEVIQHDLDMASRLDPRQI
HHHHHHHHCCCCHHHHHHHHCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCE
TTYPLMITHQTRKSVKGKLAAPQADMANQYRQILNSLNGQYNQLSAWAFGKANDEGFDEY
EEEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHH
VIDYDEYLGVGSGSFSFLNDTLYVNTFSLRKYQERIAAGQMGVEQQKNYSKKDVMQYRFL
HCCHHHHHCCCCCCCHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHH
LGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKLIGAIKNDPTDPDNLIVTDNGKMMGLL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHH
MMKEFYAGMDNVRAQLRKPLKPCDM
HHHHHHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSVIQTLNGAIATPYQANITVPNWMLGSMERVMQYYVDKNLRLDTLSAEMMPAPVEGKK
CHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCCE
YMLYAHVPFCHTLCSYCTFHRFMFKEDKARAYFISLRKEMEMVKSLGYDFESMYIGGGTT
EEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCH
TVLEDELARTIEHAKTLFPSIKEVSCESDPQHLDSPGFKQLKGLVDRMSIGVQSFNDDIL
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHH
KMTDRLDKFGTGQQTFDKIMGAKELFPIINVDLIFGFRGQTDEVIQHDLDMASRLDPRQI
HHHHHHHHCCCCHHHHHHHHCHHHHHHHEEEEEEEECCCCCHHHHHHHHHHHHCCCCCCE
TTYPLMITHQTRKSVKGKLAAPQADMANQYRQILNSLNGQYNQLSAWAFGKANDEGFDEY
EEEEEEEEECHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCCCHHH
VIDYDEYLGVGSGSFSFLNDTLYVNTFSLRKYQERIAAGQMGVEQQKNYSKKDVMQYRFL
HCCHHHHHCCCCCCCHHHCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHH
LGMFSGRLSRKYFRETFGVNLDTALFKEMTSMKLIGAIKNDPTDPDNLIVTDNGKMMGLL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHH
MMKEFYAGMDNVRAQLRKPLKPCDM
HHHHHHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]