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The map label for this gene is argA [H]

Identifier: 120597199

GI number: 120597199

Start: 394826

End: 396145

Strand: Direct

Name: argA [H]

Synonym: Sputw3181_0368

Alternate gene names: 120597199

Gene position: 394826-396145 (Clockwise)

Preceding gene: 120597197

Following gene: 120597200

Centisome position: 8.39

GC content: 47.95

Gene sequence:

>1320_bases
GTGCGTACCACTGAACTGGTTGATGGATTTCGTCATTCTGCCCCCTATGTTAATGCTCACAGAGGTAAAACTTTTGTGGT
CATGCTGGGTGGTGAAGCTTTGGCTCAAAATCAATTCCGCGCCATCTTAAATGATGTCGCCCTGCTGCACAGTTTAGGGA
TTAAAGTGGTGTTGGTATATGGCGCTCGGCCGCAGATTGATGCTGCATTGGCCGCCAACGGTATTGCGCCTGCCTACCAC
GAAGGTGTGCGTATCACCGATGAAGATTCGCTCAAAGTGATCAAGCAAGTGGCGGGTGCACTGCAATTTGATATCACGGC
GCGTTTATCCATGAGTTTAAGTAATACGCCGATGCAAGGTGCGCAAATTAACTTAGTGAGCGGCAACTTTGTGATTGCTC
AACCACTGGGTGTGGATAATGGTGTGGATTTTTGCTTGAGTGGCAAAGTGCGTCGTATCGATGTGCAAGGCTTAAAACGT
CAGTTAGATAATCACTGCATCGTATTGATGGGGCCGATTGCGGCTTCGGTTACTGGTGAAAGTTTTAATCTCACCGCTGA
GGAAATCGCGACTCAAGTGGCGATTAAGCTTAAAGCCGACAAGATGATAGGTTTTAGCTCACAGAACGGTATTCTCGATC
GTAACGGCGATGTGATTGCCGAGCTGATGCCAAATGATGCGCAAAACATTATGAACAAGCTGGCCGAACAAGGTTCTGCC
TGTGTGGGCACTATGGCCTTCTTGAAGGCGAGTATTGATGCCTGTCGTAATGGCGTGCCGCGTTGTCATTTAGTGAGTTA
TCTTGAAGATGGCGCCTTACTGCAAGAATTGTTTTCCCGTGAAGGCATAGGCACGCAAATAGTGACCGAGAGTGCCGAGC
GTTTACGCCGTGCTTCTATCGCCGATATTGGTGGCGTATTGAACCTTATTCGCCCATTGGAAGAGCAAGGTATTCTGGTG
CGTCGCAGCCGTGAGCAGCTCGAGATTGAAATCGAACAGTTTATGCTGATTGAACGTGATGGCTTAGTGATTGGCTGTGC
CGCGCTTTACCCTTTTGAAGAGGATAATGCCGGGGAGTTTGCTTGTCTTGTGGTACATCCTGATTATCGTGATGCCGACA
GAGGTAGCCTACTGCTGAAGAATATTATTGGCCAGGCTCGTAGCCGTGGTTATTCCCGTTTGTTTGCATTGACGACTCGC
AGCATTCACTGGTTTTTAGAACATGGCTTTGTGATTGAAGATGTTGATGCATTGCCGCAGAAGAAAAAGCAGTTGTATAA
CTATCAACGCCGCTCAAAAATTCTCGCACTCGATCTCTAA

Upstream 100 bases:

>100_bases
TATATGTGAATTAAAATGCAATACCATACGCGAAAATTAGCGATTCACTCTTGGCGCGTCATCGTGATGCTGCCAGCACA
AAATGAGGTGTAAATAACGC

Downstream 100 bases:

>100_bases
GTTATTCAACATTCAACGAATGCCAGCCTAGTACTCATATTAGCGCTGGCATTTTTATTGACATAATATGTCTTTGTTGG
CAGATTAAGGCTTCACTGCA

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MRTTELVDGFRHSAPYVNAHRGKTFVVMLGGEALAQNQFRAILNDVALLHSLGIKVVLVYGARPQIDAALAANGIAPAYH
EGVRITDEDSLKVIKQVAGALQFDITARLSMSLSNTPMQGAQINLVSGNFVIAQPLGVDNGVDFCLSGKVRRIDVQGLKR
QLDNHCIVLMGPIAASVTGESFNLTAEEIATQVAIKLKADKMIGFSSQNGILDRNGDVIAELMPNDAQNIMNKLAEQGSA
CVGTMAFLKASIDACRNGVPRCHLVSYLEDGALLQELFSREGIGTQIVTESAERLRRASIADIGGVLNLIRPLEEQGILV
RRSREQLEIEIEQFMLIERDGLVIGCAALYPFEEDNAGEFACLVVHPDYRDADRGSLLLKNIIGQARSRGYSRLFALTTR
SIHWFLEHGFVIEDVDALPQKKKQLYNYQRRSKILALDL

Sequences:

>Translated_439_residues
MRTTELVDGFRHSAPYVNAHRGKTFVVMLGGEALAQNQFRAILNDVALLHSLGIKVVLVYGARPQIDAALAANGIAPAYH
EGVRITDEDSLKVIKQVAGALQFDITARLSMSLSNTPMQGAQINLVSGNFVIAQPLGVDNGVDFCLSGKVRRIDVQGLKR
QLDNHCIVLMGPIAASVTGESFNLTAEEIATQVAIKLKADKMIGFSSQNGILDRNGDVIAELMPNDAQNIMNKLAEQGSA
CVGTMAFLKASIDACRNGVPRCHLVSYLEDGALLQELFSREGIGTQIVTESAERLRRASIADIGGVLNLIRPLEEQGILV
RRSREQLEIEIEQFMLIERDGLVIGCAALYPFEEDNAGEFACLVVHPDYRDADRGSLLLKNIIGQARSRGYSRLFALTTR
SIHWFLEHGFVIEDVDALPQKKKQLYNYQRRSKILALDL
>Mature_439_residues
MRTTELVDGFRHSAPYVNAHRGKTFVVMLGGEALAQNQFRAILNDVALLHSLGIKVVLVYGARPQIDAALAANGIAPAYH
EGVRITDEDSLKVIKQVAGALQFDITARLSMSLSNTPMQGAQINLVSGNFVIAQPLGVDNGVDFCLSGKVRRIDVQGLKR
QLDNHCIVLMGPIAASVTGESFNLTAEEIATQVAIKLKADKMIGFSSQNGILDRNGDVIAELMPNDAQNIMNKLAEQGSA
CVGTMAFLKASIDACRNGVPRCHLVSYLEDGALLQELFSREGIGTQIVTESAERLRRASIADIGGVLNLIRPLEEQGILV
RRSREQLEIEIEQFMLIERDGLVIGCAALYPFEEDNAGEFACLVVHPDYRDADRGSLLLKNIIGQARSRGYSRLFALTTR
SIHWFLEHGFVIEDVDALPQKKKQLYNYQRRSKILALDL

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1789181, Length=438, Percent_Identity=65.5251141552511, Blast_Score=622, Evalue=1e-179,
Organism=Saccharomyces cerevisiae, GI6320913, Length=288, Percent_Identity=22.2222222222222, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167 [H]

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.1 [H]

Molecular weight: Translated: 48139; Mature: 48139

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTELVDGFRHSAPYVNAHRGKTFVVMLGGEALAQNQFRAILNDVALLHSLGIKVVLVY
CCCHHHHHHHHHCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GARPQIDAALAANGIAPAYHEGVRITDEDSLKVIKQVAGALQFDITARLSMSLSNTPMQG
CCCCCCCHHHHCCCCCCHHHCCEEECCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCC
AQINLVSGNFVIAQPLGVDNGVDFCLSGKVRRIDVQGLKRQLDNHCIVLMGPIAASVTGE
CEEEEEECCEEEEECCCCCCCCCEEECCCEEEEEHHHHHHHHCCCEEEEEECEEEEECCC
SFNLTAEEIATQVAIKLKADKMIGFSSQNGILDRNGDVIAELMPNDAQNIMNKLAEQGSA
CCCCCHHHHHHHHHEEEEECCEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCH
CVGTMAFLKASIDACRNGVPRCHLVSYLEDGALLQELFSREGIGTQIVTESAERLRRASI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ADIGGVLNLIRPLEEQGILVRRSREQLEIEIEQFMLIERDGLVIGCAALYPFEEDNAGEF
HHHHHHHHHHHHHHHCCEEEECCCHHHHEEEEHEEEEECCCEEEEEEEECCCCCCCCCCE
ACLVVHPDYRDADRGSLLLKNIIGQARSRGYSRLFALTTRSIHWFLEHGFVIEDVDALPQ
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHCCCEEECHHHHHH
KKKQLYNYQRRSKILALDL
HHHHHHHHHHHCEEEEEEC
>Mature Secondary Structure
MRTTELVDGFRHSAPYVNAHRGKTFVVMLGGEALAQNQFRAILNDVALLHSLGIKVVLVY
CCCHHHHHHHHHCCCCEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
GARPQIDAALAANGIAPAYHEGVRITDEDSLKVIKQVAGALQFDITARLSMSLSNTPMQG
CCCCCCCHHHHCCCCCCHHHCCEEECCCHHHHHHHHHHHHHEEEEEEEEEEEECCCCCCC
AQINLVSGNFVIAQPLGVDNGVDFCLSGKVRRIDVQGLKRQLDNHCIVLMGPIAASVTGE
CEEEEEECCEEEEECCCCCCCCCEEECCCEEEEEHHHHHHHHCCCEEEEEECEEEEECCC
SFNLTAEEIATQVAIKLKADKMIGFSSQNGILDRNGDVIAELMPNDAQNIMNKLAEQGSA
CCCCCHHHHHHHHHEEEEECCEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCH
CVGTMAFLKASIDACRNGVPRCHLVSYLEDGALLQELFSREGIGTQIVTESAERLRRASI
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
ADIGGVLNLIRPLEEQGILVRRSREQLEIEIEQFMLIERDGLVIGCAALYPFEEDNAGEF
HHHHHHHHHHHHHHHCCEEEECCCHHHHEEEEHEEEEECCCEEEEEEEECCCCCCCCCCE
ACLVVHPDYRDADRGSLLLKNIIGQARSRGYSRLFALTTRSIHWFLEHGFVIEDVDALPQ
EEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHHHHHHHHCCCEEECHHHHHH
KKKQLYNYQRRSKILALDL
HHHHHHHHHHHCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]