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The map label for this gene is greB [H]

Identifier: 120596941

GI number: 120596941

Start: 133111

End: 133614

Strand: Direct

Name: greB [H]

Synonym: Sputw3181_0108

Alternate gene names: 120596941

Gene position: 133111-133614 (Clockwise)

Preceding gene: 120596937

Following gene: 120596942

Centisome position: 2.83

GC content: 44.84

Gene sequence:

>504_bases
ATGAAAGCGCATTTGATCACCCGCCAAGGTTGGTTAGCCTTAGATAAAGAATTGAAATATTTGTGGAAAGAATATCGGCC
GCAGATCACTCTTAAAGTCCAAGAGGCCGCGGCACAGGGCGATCGAAGTGAGAACGCGGATTATACTTATAACAAGCGTT
TGTTAAGACAAATTGATAGCCGTGTCAGATACTTAGTTAAGCGATTAGAAGAGTTAAAGATTGTCGACTATTCGCCGCAG
CAGGAAGGTAAAATTTACTTTGGCGCTTGGTTTGAGCTTGAAAACGACGCGGGCGAGCGGGTGCGTTATCGGATCGTCGG
TAAAGATGAGCTCGATACTAAACTGGGTTATATCACTATCGACTCGCCTATGGCGCGCGCTTTAATTGGCAAGCAAGTAG
ATGATGAAGTCGTTGTGCAAACACCATCTGGCCCAAAAGAGTGGTATATCAATGAGATCCGTTATACACCATTTGAAAAT
CCTTCGGCCAGCGTAACGGATTAA

Upstream 100 bases:

>100_bases
TTTTCTGTAGGTGGTTAACTTTAGCTGTTAGTCTGAGTATCATCTGTCCATATTTGCCCTTATCAATTGCGGCAGTTTTA
TTTAATTATCGAGTCAGAAC

Downstream 100 bases:

>100_bases
CCCTCGATGGTTATATGTAGGATGGCTTAGGTGTCCGATTATCATTGCGCAGAAGATCAAACTCTGTGATTTGATGCTGG
CTTTAGCATGAAATTGATCA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MKAHLITRQGWLALDKELKYLWKEYRPQITLKVQEAAAQGDRSENADYTYNKRLLRQIDSRVRYLVKRLEELKIVDYSPQ
QEGKIYFGAWFELENDAGERVRYRIVGKDELDTKLGYITIDSPMARALIGKQVDDEVVVQTPSGPKEWYINEIRYTPFEN
PSASVTD

Sequences:

>Translated_167_residues
MKAHLITRQGWLALDKELKYLWKEYRPQITLKVQEAAAQGDRSENADYTYNKRLLRQIDSRVRYLVKRLEELKIVDYSPQ
QEGKIYFGAWFELENDAGERVRYRIVGKDELDTKLGYITIDSPMARALIGKQVDDEVVVQTPSGPKEWYINEIRYTPFEN
PSASVTD
>Mature_167_residues
MKAHLITRQGWLALDKELKYLWKEYRPQITLKVQEAAAQGDRSENADYTYNKRLLRQIDSRVRYLVKRLEELKIVDYSPQ
QEGKIYFGAWFELENDAGERVRYRIVGKDELDTKLGYITIDSPMARALIGKQVDDEVVVQTPSGPKEWYINEIRYTPFEN
PSASVTD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=62.1794871794872, Blast_Score=198, Evalue=2e-52,
Organism=Escherichia coli, GI87082225, Length=157, Percent_Identity=35.6687898089172, Blast_Score=98, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 19563; Mature: 19563

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAHLITRQGWLALDKELKYLWKEYRPQITLKVQEAAAQGDRSENADYTYNKRLLRQIDS
CCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLVKRLEELKIVDYSPQQEGKIYFGAWFELENDAGERVRYRIVGKDELDTKLGYITI
HHHHHHHHHHHCEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEE
DSPMARALIGKQVDDEVVVQTPSGPKEWYINEIRYTPFENPSASVTD
CCHHHHHHHCCCCCCCEEEECCCCCHHEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKAHLITRQGWLALDKELKYLWKEYRPQITLKVQEAAAQGDRSENADYTYNKRLLRQIDS
CCCEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLVKRLEELKIVDYSPQQEGKIYFGAWFELENDAGERVRYRIVGKDELDTKLGYITI
HHHHHHHHHHHCEEECCCCCCCCEEEEEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEE
DSPMARALIGKQVDDEVVVQTPSGPKEWYINEIRYTPFENPSASVTD
CCHHHHHHHCCCCCCCEEEECCCCCHHEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]