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The map label for this gene is rfaB [H]

Identifier: 120596888

GI number: 120596888

Start: 68961

End: 70046

Strand: Direct

Name: rfaB [H]

Synonym: Sputw3181_0055

Alternate gene names: 120596888

Gene position: 68961-70046 (Clockwise)

Preceding gene: 120596887

Following gene: 120596889

Centisome position: 1.46

GC content: 41.44

Gene sequence:

>1086_bases
ATGCGGATAGCCATTGCCATCGACAGTTTAGCGGGTGGCGGAGCCGAAAAAGTCATGATTACCTTAGCCAAGCAATTTAT
TCGGCTAGGGCATGAACCGCATTTTTTAGTCATGGAAGATAATCGGTATTATGAAACGCCGGAAGACTTACCCATTCATC
AATGTTTTGCCCCTAAGGATAAAGATTTCGACCATTTTGGGCGGTTAAATGCTTCGGTACACAAACTCAGCGCAAAAATC
GCTGAAATCGAATCTCAAGTGGGTAAGTTCGACTTGTTTTTATCTAACTTAGATAAAACCAATTTGATGATGACCAAAAC
AGATGTTTCACCACTTTATGTTATAGTCCATTCTTCAGTAGAAGAAGAGCTTAGTCGCCAATTCAAATTGGGGCCGTTTG
CTTATTTTAAGAAGTTAAGAGCTAAAAAAGCGCTCAATGGTCAGCATTTGATTACAGTCTCAAATGGCATAGCAAAAGAG
ATCAAAGATAAAGGCCGCTTGCATCCAGCATCTATGCAGACTATCTATAATCCATTTGAATTTAATGAAATCACATCTCA
ATCACAGCAAGATAATCCACAAATTCCTAAGGGTGATTATCTTATTCATGTCGGCCGCTTTGCCAAGCAAAAACGCCATG
ATGTGTTGTTTGAAGCCCTTAAGCTAACGCGAAATCAACTTCCTGTAGTGCTCTTGTGTCATAACCACAAAAAAGCGATT
AAAGCGGCGAAAAAGTTCGGTATTGAAAAACGTTTGATTATCCCCGGTTTTCAAAGCAATCCCTTTCCATGGATTAAGCA
GGCTAAGGCATTAGTGCTCAGCTCAGATTTTGAAGGTCTACCGACAGTCTTGATTGAATCTTTAGCTTGTGGGACTCCTG
TTGTGAGTACGGATTGCCCTCATGGGCCGAATGAAATTTTAACTGGAAATTTAGTGTCTTATCTCGTACCGCGTCGCGAT
CCAAAAGCGTTGTCCAAAATGATAGACGAGGTTGTTAGCCAGCCGCCATCCCTCGAGCAGGTTGAGATTTTGGCCAAAGT
CGATGCGACCTTAATTGCCGAACAATATTTAGCGTTGGCAAAATAA

Upstream 100 bases:

>100_bases
GCACTCAGATAAGACATGTTAGTGTAGTGCATTCTTCAGCACTCTAGATGTATCGCTGCATTAGCGGTTGGTATTTAGAA
TATCGTTTAGGAGATAAATC

Downstream 100 bases:

>100_bases
TTTATTCGTTTGTGTTTTAGGAGCATGCTTGTGGAAGAAGTTAAACAAGCCAAAAGACGTTACTTATTGTACATAGCGCA
GAACTATTCCTATGCGATGT

Product: glycosyl transferase, group 1

Products: NA

Alternate protein names: UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRIAIAIDSLAGGGAEKVMITLAKQFIRLGHEPHFLVMEDNRYYETPEDLPIHQCFAPKDKDFDHFGRLNASVHKLSAKI
AEIESQVGKFDLFLSNLDKTNLMMTKTDVSPLYVIVHSSVEEELSRQFKLGPFAYFKKLRAKKALNGQHLITVSNGIAKE
IKDKGRLHPASMQTIYNPFEFNEITSQSQQDNPQIPKGDYLIHVGRFAKQKRHDVLFEALKLTRNQLPVVLLCHNHKKAI
KAAKKFGIEKRLIIPGFQSNPFPWIKQAKALVLSSDFEGLPTVLIESLACGTPVVSTDCPHGPNEILTGNLVSYLVPRRD
PKALSKMIDEVVSQPPSLEQVEILAKVDATLIAEQYLALAK

Sequences:

>Translated_361_residues
MRIAIAIDSLAGGGAEKVMITLAKQFIRLGHEPHFLVMEDNRYYETPEDLPIHQCFAPKDKDFDHFGRLNASVHKLSAKI
AEIESQVGKFDLFLSNLDKTNLMMTKTDVSPLYVIVHSSVEEELSRQFKLGPFAYFKKLRAKKALNGQHLITVSNGIAKE
IKDKGRLHPASMQTIYNPFEFNEITSQSQQDNPQIPKGDYLIHVGRFAKQKRHDVLFEALKLTRNQLPVVLLCHNHKKAI
KAAKKFGIEKRLIIPGFQSNPFPWIKQAKALVLSSDFEGLPTVLIESLACGTPVVSTDCPHGPNEILTGNLVSYLVPRRD
PKALSKMIDEVVSQPPSLEQVEILAKVDATLIAEQYLALAK
>Mature_361_residues
MRIAIAIDSLAGGGAEKVMITLAKQFIRLGHEPHFLVMEDNRYYETPEDLPIHQCFAPKDKDFDHFGRLNASVHKLSAKI
AEIESQVGKFDLFLSNLDKTNLMMTKTDVSPLYVIVHSSVEEELSRQFKLGPFAYFKKLRAKKALNGQHLITVSNGIAKE
IKDKGRLHPASMQTIYNPFEFNEITSQSQQDNPQIPKGDYLIHVGRFAKQKRHDVLFEALKLTRNQLPVVLLCHNHKKAI
KAAKKFGIEKRLIIPGFQSNPFPWIKQAKALVLSSDFEGLPTVLIESLACGTPVVSTDCPHGPNEILTGNLVSYLVPRRD
PKALSKMIDEVVSQPPSLEQVEILAKVDATLIAEQYLALAK

Specific function: Adds a galactose goup to a glucose group of LPS [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=242, Percent_Identity=25.2066115702479, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 40455; Mature: 40455

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAIAIDSLAGGGAEKVMITLAKQFIRLGHEPHFLVMEDNRYYETPEDLPIHQCFAPKD
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
KDFDHFGRLNASVHKLSAKIAEIESQVGKFDLFLSNLDKTNLMMTKTDVSPLYVIVHSSV
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECHH
EEELSRQFKLGPFAYFKKLRAKKALNGQHLITVSNGIAKEIKDKGRLHPASMQTIYNPFE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHCCCHH
FNEITSQSQQDNPQIPKGDYLIHVGRFAKQKRHDVLFEALKLTRNQLPVVLLCHNHKKAI
HHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
KAAKKFGIEKRLIIPGFQSNPFPWIKQAKALVLSSDFEGLPTVLIESLACGTPVVSTDCP
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCCC
HGPNEILTGNLVSYLVPRRDPKALSKMIDEVVSQPPSLEQVEILAKVDATLIAEQYLALA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MRIAIAIDSLAGGGAEKVMITLAKQFIRLGHEPHFLVMEDNRYYETPEDLPIHQCFAPKD
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHCCCCC
KDFDHFGRLNASVHKLSAKIAEIESQVGKFDLFLSNLDKTNLMMTKTDVSPLYVIVHSSV
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEEEECHH
EEELSRQFKLGPFAYFKKLRAKKALNGQHLITVSNGIAKEIKDKGRLHPASMQTIYNPFE
HHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHCCCHH
FNEITSQSQQDNPQIPKGDYLIHVGRFAKQKRHDVLFEALKLTRNQLPVVLLCHNHKKAI
HHHHHCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHH
KAAKKFGIEKRLIIPGFQSNPFPWIKQAKALVLSSDFEGLPTVLIESLACGTPVVSTDCP
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCCC
HGPNEILTGNLVSYLVPRRDPKALSKMIDEVVSQPPSLEQVEILAKVDATLIAEQYLALA
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1624461; 8041620; 9278503 [H]