Click here to switch to the map view.

The map label for this gene is tag [H]

Identifier: 120596841

GI number: 120596841

Start: 10808

End: 11410

Strand: Direct

Name: tag [H]

Synonym: Sputw3181_0008

Alternate gene names: 120596841

Gene position: 10808-11410 (Clockwise)

Preceding gene: 120596838

Following gene: 120596847

Centisome position: 0.23

GC content: 45.11

Gene sequence:

>603_bases
ATGGAAGAGATCCGCTGTGGCTGGGTAAGTGATGATCCCTTGTATCGTGAGTATCACGACAAAGTGTGGGGGCGGCCAGT
TTATGATTCTAAGGAGTTGTTTGCTAAGTTGTGTTTAGATGGTCAACAGGCCGGATTATCTTGGATTACGATCCTGAAGA
AGCAACAAAACTATGAGCAGGCCTTTGCCAATTTTGAACCTGAGATAATTGCCACCTTCGATGAGACAAAAGTAGAGGAG
CTTATGTTGAATCCCGGTATAGTGCGCAATCGCTTGAAGGTGAACTCTATCATCAAGAATGCCAAAGGTTATTTAGCCTA
TACTGCCGATGGGAAGGATTTCTCTGCATTTTTATGGAGCTTTGTGGGCGGTAAACCCATAGTTAATCAGATTAGGGCAA
TGTCGCAGGTTCCGGCACAAACACCAGAATCTGAAGCCATGTCAAAAGCGTTGAAGAAGCTCGGATTCAATTTTGTCGGC
CCAACCATTTGTTATGCCTTTATGCAGGCGGTGGGAATGGTGAACGATCATCTTGTCGAGTGTATTGCTTATCAGGCCTG
TGTCGATTCTTGTGCCGGACTGCATAATCACGAGCAACATTAA

Upstream 100 bases:

>100_bases
CGTCAGCCTGAATAGAAAATGGGTTCGATTATACCTTGTAGATATCTGCTTATGTAGGTTATTTTTTACCTTCTTAATTA
AAACAATAAGGTTATATACC

Downstream 100 bases:

>100_bases
TCGAGAGATTGCCGATGTTGAAGTAGGGTTTAGAACTTGTCGTGGTATTAGAAGTCTAGAGATAAAAAAAGCATGTTCCA
CGTGGAACATGCTTTTGGTT

Product: DNA-3-methyladenine glycosylase I

Products: NA

Alternate protein names: 3-methyladenine-DNA glycosylase I, constitutive; TAG I; DNA-3-methyladenine glycosidase I; DNA-3-methyladenine glycosylase I [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDSKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEIIATFDETKVEE
LMLNPGIVRNRLKVNSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQIRAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECIAYQACVDSCAGLHNHEQH

Sequences:

>Translated_200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDSKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEIIATFDETKVEE
LMLNPGIVRNRLKVNSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQIRAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECIAYQACVDSCAGLHNHEQH
>Mature_200_residues
MEEIRCGWVSDDPLYREYHDKVWGRPVYDSKELFAKLCLDGQQAGLSWITILKKQQNYEQAFANFEPEIIATFDETKVEE
LMLNPGIVRNRLKVNSIIKNAKGYLAYTADGKDFSAFLWSFVGGKPIVNQIRAMSQVPAQTPESEAMSKALKKLGFNFVG
PTICYAFMQAVGMVNDHLVECIAYQACVDSCAGLHNHEQH

Specific function: Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions [H]

COG id: COG2818

COG function: function code L; 3-methyladenine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789971, Length=180, Percent_Identity=55, Blast_Score=216, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005019
- InterPro:   IPR011257
- InterPro:   IPR004597 [H]

Pfam domain/function: PF03352 Adenine_glyco [H]

EC number: =3.2.2.20 [H]

Molecular weight: Translated: 22591; Mature: 22591

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEIRCGWVSDDPLYREYHDKVWGRPVYDSKELFAKLCLDGQQAGLSWITILKKQQNYEQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AFANFEPEIIATFDETKVEELMLNPGIVRNRLKVNSIIKNAKGYLAYTADGKDFSAFLWS
HHHCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
FVGGKPIVNQIRAMSQVPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHLVE
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CIAYQACVDSCAGLHNHEQH
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEEIRCGWVSDDPLYREYHDKVWGRPVYDSKELFAKLCLDGQQAGLSWITILKKQQNYEQ
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
AFANFEPEIIATFDETKVEELMLNPGIVRNRLKVNSIIKNAKGYLAYTADGKDFSAFLWS
HHHCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
FVGGKPIVNQIRAMSQVPAQTPESEAMSKALKKLGFNFVGPTICYAFMQAVGMVNDHLVE
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
CIAYQACVDSCAGLHNHEQH
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3536912; 3520491; 8041620; 9278503 [H]