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The map label for this gene is glyS

Identifier: 119947319

GI number: 119947319

Start: 4542763

End: 4544829

Strand: Reverse

Name: glyS

Synonym: Ping_3726

Alternate gene names: 119947319

Gene position: 4544829-4542763 (Counterclockwise)

Preceding gene: 119947320

Following gene: 119947318

Centisome position: 99.68

GC content: 41.41

Gene sequence:

>2067_bases
ATGGCACAAGAAAATTTATTGATTGAACTGGGAACTGAAGAGTTACCGCCCAAATCACTGCGTCAACTTGCTGAATCTTT
TGCAAGTAATGTTGAAGCTGAATTAAATAAAGCTGAATTATCCTTTGATAGCGTCCGATGGCTAGCATCTCCGCGTCGTT
TGGCATTAGTTATTGCTAACTTATCAGACTCGCAAGCGGATAAAATAGTAGAAAAACGTGGTCCTGCGGTAAATGTTGCC
TTTGATGCCGAAGGTCAGGCGACCAAAGCTGCACAGGGCTGGGCGCGTTCAAACGGTATTACCGTTGAACAAGCTGAGCG
TCTGATCACTGATAAAGGGGAATGGTTATTATTCAAATCCGAGGTTAAAGGGTTATCCGTGGCGGAGCTGATTCCTGAAA
TCGCCGCAAATGCACTGGCCAAGTTGCCTATTTCCAAGCCGATGCGCTGGGGATCGAGCAGTACGCAATTTATCCGCCCT
GTTCATACTGTGACTATGTTATTTGGTTCGCGGTTAATTCAAGGCGAGTTATTAGGTGTTGCATCGGATCGCATTATTCG
TGGCCATCGTTTTCTAGGCGAAGCTGAATTGATTATTGATCATGCTGATCAATACGAAACATTATTAGATGATAGCGGTA
AAGTGATTGTTGATTATGAACGCCGTAAAGCGATTATTCGTGATCAGGTTGAAGCATTGGCGGCCCATGAAAATGGTGTT
GCAGATATTGATGAAAGTCTACTTGAAGAGGTGACCTCATTAGTTGAATGGCCAGTAACCTTGGTTGGGTCCTTTGAAGA
TAAATTTTTAGACGTCCCTTCCGAAGCCCTTATCTATACAATGAAAGATAATCAAAAATACTTCCCGGTATTAGATAAAG
ACGGCAAACTGTTACCGCGCTTTATTTTTGTTTCTAATATTGTCAGTCGAGATCCTGCACAAGTCATAAGCGGTAACGAA
AAAGTGATTCGTCCACGTTTAGCGGATGCTGAATTCTTTTTTGAGACAGATAAGAAAAAAACCTTAGCAAGCCGTTTAGA
ATCTTTATCCAGTGTTTTATTTCAACAAAAATTAGGGACGTTAAAAGAAAAATCTGAACGTATTGCTAATGTCGCTGAAG
ATATCGCTTTGCAAATTAAAGCCGATACAAAACATGCCCAACGTGCCGGTCTTTTATCTAAAACAGATTTAATGACCGAT
ATGGTGATGGAGTTCCCCGATGTGCAAGGCATTATGGGAATGCATTACGCGTTACATGATGGTGAAGAGCAGGAAGTTGC
TATTGCCTTAAATGAGCAATACTTACCGCGTTTTGCCGGTGATAAATTACCCACTTCTTTAGTGGCATGTGCTGTTTCTT
TAGCAGATAAACTCGATACTTTAGTGGGTATTTTTGGTATTGGTCAAGCACCCAAAGGTGCTGCTGATCCCTTTGCACTG
CGTCGTGCTGCAATCGGTTTATTACGTATTATTACTAATAAAAATCTGGATCTGGATCTGGTCGAATTAGTTGAAATTGC
GAAACTGCAATATGGTCATAAATTAACTAATGATAACGTGGTACAGGATGTTGTTGATTTCTTGTTTGCCCGATTCCGTG
CGACTTATCAAGCTAACGGTTATTCAGTTGAGCTGATTCAATCGGTACTGGTTAGAAGGCCTACCAAGCCTGTTGATTTT
GAGAAACGTCTTCAAGCTGTTGCCAAGTTCCAAACATTACCGGAAGCGGCTCCTATTGCCGCCGCCAATAAACGTATTAG
TAATATATTAGCTAAAGTCAAAGGGGAGATAAATGCACAGGTAGACCCTTCTTTACTGCAGGAGCCCGCTGAAATCAAAT
TATCGGAAATTTTAGGCTCTCTAGAATCAACACTAAGGCCTTTATTTGACAACTTAGACTACGAGTCAGCCTTGTTTGAA
CTCGCGTCTCTAAACGAGCCTGTGGACGAATTCTTTGATAACGTAATGGTAATGGCCGAAGATCCTGCTATCAAAGCGAA
CCGTCTTGCGATTTTAAATCGTTTGCGTAACTTATTTTTACAAATTGCCGATGTTTCTGTTTTGTAA

Upstream 100 bases:

>100_bases
TTCGTGCATTATCAAAAGCAGTCGCTGAAGCATATTACGGCAAGCGTGAAGCGCTTGGTTTCCCACTTTGCAAAAACCTA
GTTAAATAGAGGCAAGTTTC

Downstream 100 bases:

>100_bases
AAAATCATCGCAGTGCTGTGCAGATAAGTAATATATTGATTAAAAAGCTCAGTTTATACTGAGCTTTTTTGTTACAGATA
AAAACGTCTACTTCTTGCTC

Product: glycyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAQENLLIELGTEELPPKSLRQLAESFASNVEAELNKAELSFDSVRWLASPRRLALVIANLSDSQADKIVEKRGPAVNVA
FDAEGQATKAAQGWARSNGITVEQAERLITDKGEWLLFKSEVKGLSVAELIPEIAANALAKLPISKPMRWGSSSTQFIRP
VHTVTMLFGSRLIQGELLGVASDRIIRGHRFLGEAELIIDHADQYETLLDDSGKVIVDYERRKAIIRDQVEALAAHENGV
ADIDESLLEEVTSLVEWPVTLVGSFEDKFLDVPSEALIYTMKDNQKYFPVLDKDGKLLPRFIFVSNIVSRDPAQVISGNE
KVIRPRLADAEFFFETDKKKTLASRLESLSSVLFQQKLGTLKEKSERIANVAEDIALQIKADTKHAQRAGLLSKTDLMTD
MVMEFPDVQGIMGMHYALHDGEEQEVAIALNEQYLPRFAGDKLPTSLVACAVSLADKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGLLRIITNKNLDLDLVELVEIAKLQYGHKLTNDNVVQDVVDFLFARFRATYQANGYSVELIQSVLVRRPTKPVDF
EKRLQAVAKFQTLPEAAPIAAANKRISNILAKVKGEINAQVDPSLLQEPAEIKLSEILGSLESTLRPLFDNLDYESALFE
LASLNEPVDEFFDNVMVMAEDPAIKANRLAILNRLRNLFLQIADVSVL

Sequences:

>Translated_688_residues
MAQENLLIELGTEELPPKSLRQLAESFASNVEAELNKAELSFDSVRWLASPRRLALVIANLSDSQADKIVEKRGPAVNVA
FDAEGQATKAAQGWARSNGITVEQAERLITDKGEWLLFKSEVKGLSVAELIPEIAANALAKLPISKPMRWGSSSTQFIRP
VHTVTMLFGSRLIQGELLGVASDRIIRGHRFLGEAELIIDHADQYETLLDDSGKVIVDYERRKAIIRDQVEALAAHENGV
ADIDESLLEEVTSLVEWPVTLVGSFEDKFLDVPSEALIYTMKDNQKYFPVLDKDGKLLPRFIFVSNIVSRDPAQVISGNE
KVIRPRLADAEFFFETDKKKTLASRLESLSSVLFQQKLGTLKEKSERIANVAEDIALQIKADTKHAQRAGLLSKTDLMTD
MVMEFPDVQGIMGMHYALHDGEEQEVAIALNEQYLPRFAGDKLPTSLVACAVSLADKLDTLVGIFGIGQAPKGAADPFAL
RRAAIGLLRIITNKNLDLDLVELVEIAKLQYGHKLTNDNVVQDVVDFLFARFRATYQANGYSVELIQSVLVRRPTKPVDF
EKRLQAVAKFQTLPEAAPIAAANKRISNILAKVKGEINAQVDPSLLQEPAEIKLSEILGSLESTLRPLFDNLDYESALFE
LASLNEPVDEFFDNVMVMAEDPAIKANRLAILNRLRNLFLQIADVSVL
>Mature_687_residues
AQENLLIELGTEELPPKSLRQLAESFASNVEAELNKAELSFDSVRWLASPRRLALVIANLSDSQADKIVEKRGPAVNVAF
DAEGQATKAAQGWARSNGITVEQAERLITDKGEWLLFKSEVKGLSVAELIPEIAANALAKLPISKPMRWGSSSTQFIRPV
HTVTMLFGSRLIQGELLGVASDRIIRGHRFLGEAELIIDHADQYETLLDDSGKVIVDYERRKAIIRDQVEALAAHENGVA
DIDESLLEEVTSLVEWPVTLVGSFEDKFLDVPSEALIYTMKDNQKYFPVLDKDGKLLPRFIFVSNIVSRDPAQVISGNEK
VIRPRLADAEFFFETDKKKTLASRLESLSSVLFQQKLGTLKEKSERIANVAEDIALQIKADTKHAQRAGLLSKTDLMTDM
VMEFPDVQGIMGMHYALHDGEEQEVAIALNEQYLPRFAGDKLPTSLVACAVSLADKLDTLVGIFGIGQAPKGAADPFALR
RAAIGLLRIITNKNLDLDLVELVEIAKLQYGHKLTNDNVVQDVVDFLFARFRATYQANGYSVELIQSVLVRRPTKPVDFE
KRLQAVAKFQTLPEAAPIAAANKRISNILAKVKGEINAQVDPSLLQEPAEIKLSEILGSLESTLRPLFDNLDYESALFEL
ASLNEPVDEFFDNVMVMAEDPAIKANRLAILNRLRNLFLQIADVSVL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=688, Percent_Identity=61.6279069767442, Blast_Score=863, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_PSYIN (A1T0Y5)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944999.1
- ProteinModelPortal:   A1T0Y5
- STRING:   A1T0Y5
- GeneID:   4624875
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3726
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   A1T0Y5
- BioCyc:   PING357804:PING_3726-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76177; Mature: 76046

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQENLLIELGTEELPPKSLRQLAESFASNVEAELNKAELSFDSVRWLASPRRLALVIAN
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCEEEEEEEC
LSDSQADKIVEKRGPAVNVAFDAEGQATKAAQGWARSNGITVEQAERLITDKGEWLLFKS
CCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHCHHHCCCCCHHHHHHHHCCCCCEEEEHH
EVKGLSVAELIPEIAANALAKLPISKPMRWGSSSTQFIRPVHTVTMLFGSRLIQGELLGV
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASDRIIRGHRFLGEAELIIDHADQYETLLDDSGKVIVDYERRKAIIRDQVEALAAHENGV
HHHHHHHHHHHCCCHHHEEECHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
ADIDESLLEEVTSLVEWPVTLVGSFEDKFLDVPSEALIYTMKDNQKYFPVLDKDGKLLPR
HHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCHHEEEEEECCCEECCEECCCCCHHHH
FIFVSNIVSRDPAQVISGNEKVIRPRLADAEFFFETDKKKTLASRLESLSSVLFQQKLGT
HHHHHHHHCCCCHHHHCCCCCEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LKEKSERIANVAEDIALQIKADTKHAQRAGLLSKTDLMTDMVMEFPDVQGIMGMHYALHD
HHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
GEEQEVAIALNEQYLPRFAGDKLPTSLVACAVSLADKLDTLVGIFGIGQAPKGAADPFAL
CCCCEEEEEECCHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGLLRIITNKNLDLDLVELVEIAKLQYGHKLTNDNVVQDVVDFLFARFRATYQANG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YSVELIQSVLVRRPTKPVDFEKRLQAVAKFQTLPEAAPIAAANKRISNILAKVKGEINAQ
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VDPSLLQEPAEIKLSEILGSLESTLRPLFDNLDYESALFELASLNEPVDEFFDNVMVMAE
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCEEEEEC
DPAIKANRLAILNRLRNLFLQIADVSVL
CCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AQENLLIELGTEELPPKSLRQLAESFASNVEAELNKAELSFDSVRWLASPRRLALVIAN
CCCCEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCEEEEEEEC
LSDSQADKIVEKRGPAVNVAFDAEGQATKAAQGWARSNGITVEQAERLITDKGEWLLFKS
CCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHCHHHCCCCCHHHHHHHHCCCCCEEEEHH
EVKGLSVAELIPEIAANALAKLPISKPMRWGSSSTQFIRPVHTVTMLFGSRLIQGELLGV
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASDRIIRGHRFLGEAELIIDHADQYETLLDDSGKVIVDYERRKAIIRDQVEALAAHENGV
HHHHHHHHHHHCCCHHHEEECHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
ADIDESLLEEVTSLVEWPVTLVGSFEDKFLDVPSEALIYTMKDNQKYFPVLDKDGKLLPR
HHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCHHEEEEEECCCEECCEECCCCCHHHH
FIFVSNIVSRDPAQVISGNEKVIRPRLADAEFFFETDKKKTLASRLESLSSVLFQQKLGT
HHHHHHHHCCCCHHHHCCCCCEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LKEKSERIANVAEDIALQIKADTKHAQRAGLLSKTDLMTDMVMEFPDVQGIMGMHYALHD
HHHHHHHHHHHHHHHEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
GEEQEVAIALNEQYLPRFAGDKLPTSLVACAVSLADKLDTLVGIFGIGQAPKGAADPFAL
CCCCEEEEEECCHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAAIGLLRIITNKNLDLDLVELVEIAKLQYGHKLTNDNVVQDVVDFLFARFRATYQANG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
YSVELIQSVLVRRPTKPVDFEKRLQAVAKFQTLPEAAPIAAANKRISNILAKVKGEINAQ
CHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
VDPSLLQEPAEIKLSEILGSLESTLRPLFDNLDYESALFELASLNEPVDEFFDNVMVMAE
CCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCEEEEEC
DPAIKANRLAILNRLRNLFLQIADVSVL
CCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA