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The map label for this gene is lafS [H]

Identifier: 119947183

GI number: 119947183

Start: 4397265

End: 4397996

Strand: Reverse

Name: lafS [H]

Synonym: Ping_3581

Alternate gene names: 119947183

Gene position: 4397996-4397265 (Counterclockwise)

Preceding gene: 119947184

Following gene: 119947182

Centisome position: 96.46

GC content: 40.57

Gene sequence:

>732_bases
ATGCTGCACTCAACTAAGCAAAAAAAATATAATCAGACGGCTGAGCTGACAGGAACAAGAAATAAGCAGGAAGAATCTTT
TCTGGTGAACAAATATCTCTATCTGGTACATGCGGTGATAAAAAAAATGGGCTATATCAACTGCATTTCAGACAACGATG
ATATGAAGCAAGTGGGGCTGATTGCCCTATTGAAGATGATTCGCAGATTCCCTGTTATGGATGACGAAAAAGAATTTATC
CGCCTCACTTATATCCGTATACGGGGCTCGATACTCGATGAGATCCGAAGCCGTGACTGGCGCTCCGAGGAAAGCCGAAA
AAATGCCAGTACATTTTTGGCGCAGGAAAAAGCCCTGATGCAGTCCTTGGGTCGATTACCAAACAAGTCGGAAATGCTAG
AGAATAAGGTACTCAGTGAACAACAATACGAAGAAGCCCGTTGCGCTATAAACGGCAAAGTTTTTTCCGAGCTGAGTGAT
CAACTGCTAAAGGACAATGCTCAGAGTGTAGACATCTGCACCGAAATTGATAATACACAATGTCTGAGTGTGGCTTTAAA
ACAGCTTACGGAAGAGGAAGTTTTGATTTTACATCTGATGTATGAAAAAGATATGTCGTTTAAAGAGATGGCATTAGCGA
CTAATAATAATTTCTCCCGAATCAATAAAATCCAGCGAGAGGCTCATCGAAAATTGACATATTTAGTTGTAAATAAAGGG
GGTTTATTATGA

Upstream 100 bases:

>100_bases
TTTAATAGCGATGTAAATCCAATACTCAAAAAAATCTCATCAGCTGGTGCATATTCTCAAGTGCATGTGACCAAGCTAAT
TATTCAGTAGGTGATAGGAG

Downstream 100 bases:

>100_bases
TCCGGTTAGCAGGCCTAGTTTTTATTATGTTTTGCCTATTTGGGGGTTTCATGATTGTTGGCGGGAATCTAAAGTTGTTA
TGGAAACCCACTGAAATTTT

Product: sigma-28 (sigma F) factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MLHSTKQKKYNQTAELTGTRNKQEESFLVNKYLYLVHAVIKKMGYINCISDNDDMKQVGLIALLKMIRRFPVMDDEKEFI
RLTYIRIRGSILDEIRSRDWRSEESRKNASTFLAQEKALMQSLGRLPNKSEMLENKVLSEQQYEEARCAINGKVFSELSD
QLLKDNAQSVDICTEIDNTQCLSVALKQLTEEEVLILHLMYEKDMSFKEMALATNNNFSRINKIQREAHRKLTYLVVNKG
GLL

Sequences:

>Translated_243_residues
MLHSTKQKKYNQTAELTGTRNKQEESFLVNKYLYLVHAVIKKMGYINCISDNDDMKQVGLIALLKMIRRFPVMDDEKEFI
RLTYIRIRGSILDEIRSRDWRSEESRKNASTFLAQEKALMQSLGRLPNKSEMLENKVLSEQQYEEARCAINGKVFSELSD
QLLKDNAQSVDICTEIDNTQCLSVALKQLTEEEVLILHLMYEKDMSFKEMALATNNNFSRINKIQREAHRKLTYLVVNKG
GLL
>Mature_243_residues
MLHSTKQKKYNQTAELTGTRNKQEESFLVNKYLYLVHAVIKKMGYINCISDNDDMKQVGLIALLKMIRRFPVMDDEKEFI
RLTYIRIRGSILDEIRSRDWRSEESRKNASTFLAQEKALMQSLGRLPNKSEMLENKVLSEQQYEEARCAINGKVFSELSD
QLLKDNAQSVDICTEIDNTQCLSVALKQLTEEEVLILHLMYEKDMSFKEMALATNNNFSRINKIQREAHRKLTYLVVNKG
GLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=220, Percent_Identity=26.8181818181818, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 28251; Mature: 28251

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSTKQKKYNQTAELTGTRNKQEESFLVNKYLYLVHAVIKKMGYINCISDNDDMKQVGL
CCCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
IALLKMIRRFPVMDDEKEFIRLTYIRIRGSILDEIRSRDWRSEESRKNASTFLAQEKALM
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QSLGRLPNKSEMLENKVLSEQQYEEARCAINGKVFSELSDQLLKDNAQSVDICTEIDNTQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEHHHCCCCHH
CLSVALKQLTEEEVLILHLMYEKDMSFKEMALATNNNFSRINKIQREAHRKLTYLVVNKG
HHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCC
GLL
CCC
>Mature Secondary Structure
MLHSTKQKKYNQTAELTGTRNKQEESFLVNKYLYLVHAVIKKMGYINCISDNDDMKQVGL
CCCCCHHHHHCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
IALLKMIRRFPVMDDEKEFIRLTYIRIRGSILDEIRSRDWRSEESRKNASTFLAQEKALM
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
QSLGRLPNKSEMLENKVLSEQQYEEARCAINGKVFSELSDQLLKDNAQSVDICTEIDNTQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEHHHCCCCHH
CLSVALKQLTEEEVLILHLMYEKDMSFKEMALATNNNFSRINKIQREAHRKLTYLVVNKG
HHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEECCC
GLL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]