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The map label for this gene is cysI

Identifier: 119947038

GI number: 119947038

Start: 4246721

End: 4248409

Strand: Direct

Name: cysI

Synonym: Ping_3435

Alternate gene names: 119947038

Gene position: 4246721-4248409 (Clockwise)

Preceding gene: 119947037

Following gene: 119947039

Centisome position: 93.14

GC content: 42.92

Gene sequence:

>1689_bases
ATGAGCAAGAAATTACACGATAACGAACGTATTAAAATTGAAAGTAACTTTCTTCGCGGTAAATTAGTGGAAGGACTGAA
TGATAATACTACCGGTGCCTTTATACCTGATGATGTTCAGTTGGTTAAGTTTCATGGTTTATATCAACAGGATCATCGCG
ACTATCGCGCTGAGCGTAAAAAACAGAAATTAGAGCCTTTATACTCTTTTCTGTTGCGCGCCCGTTTACCCGGTGGGGTT
GTTACTCCACAGCAGTTATTAGAGATTGACCGGATTGCCAGTGAATTAACCGATGCCGGCAGTATCCGCTTGACCACCCG
TCAAACGTTCCAGTATCACGGCATCTATAAACGCAATCTAAAGTCAGTTTTTCAGGAACTTAAGTTCAAAGCGAACATTG
ATTCTATCTTTACGGCGGGTGATGTTAACCGTAATGTTATTTGTACCTCTAACCCAATGCAGTCTGAACTGCATGCAGAG
GTATATGACTGGTCGACGAAAATAAGTGAAATGTTATTACCCCACAGTAATGCCTTTGCTGAAATTTGGTTAGACGGTGA
AAAAGTGCATGAAACCGAACAAGAACCAATTTATGGCGAAACCATGTTACCGCGTAAATTTAAAACAACCGTTGTTTTGC
CTCCCTTTAACGATGTGGATGTGCACGCCAATGATCTTAACTTTGTGGCGATCGTTGAAAACGAAAAAATAGTGGGTTTT
AACGTATTAGTGGGTGGTGGTTTAGGAATAACCCACGGTGATAAAACTACTTTCCCGCGTAAAGCAGATGATTTTGGGTT
TATTGCGGCAGAAGATATTTTAGAAATTGCCAAGGCAGTGGTCACCACCCAACGTGATTTAGGTGGACGTACTAGCCGTT
CTCACGCACGTACAAAATACACTTTAGAAACTCATGGTGTAGAAAAATTTAAGGGTGAAGTGGAATCACGCTCTGGCATT
AAATTTTTGCCATCACGCCCCTATGAATTTACCGAACGAGGCGATCGCATCGGTTGGATAAAAGGGACAGATAAGAAATG
GCATTTAACAGTATTTATTGAAAATGGCCGTATTCTGGATTACCCGGGTAAACCGCTTAAATCCGGTTTAGTTGAAATTG
CAAAAGTTCATCAGGGTGATTTCCGCATGACAGCGAATCAAAACCTGATCATTGCTAGTGTACCGGAATCTGAAAAAGAA
AAAATTGAAGCATTGGCGCGTAAATGCGGTCTGATCAATGATGCTCACTCTAAGCAGCGTTTTAATTCTATGGCCTGTGT
AGCATTACCGACCTGCCCGCTGGCGATGGCGGAAGCTGAGCGCTACCTGCCGGGGTTAGTCACGGATGTGGAGGCATTGC
TCACTAAGCATGGTATTGGCGACGACCATATTATTTTAAGGGTGACGGGTTGCCCGAATGGTTGTGCTCGTCCTCTGCTT
GCTGAAGTCGGCTTAATAGGTAAAGCGCCGGGCCGTTATAACCTTTATCTTGGCGGTAATACCAACGGTACCCGCTTGCC
TAAGATGTACAAAGAAAATATTGATGAAGCAGCCATTATAATGACCATGGATGAGTTAATTGGTCGTTGGGCAAATGAGC
GTGAAAGCGGTGAATGTTTTGGTGATTTTGTTATCCGCGCTGCTATCATAGCTGAAGTTATTATCGCTTCTAAGGATTTT
CATGACTAA

Upstream 100 bases:

>100_bases
TCGAAATTATCGGCTCTCAAGGCAAGAAAAGCCTCGAAGATGCAGAGCAATATTTAACTGAATTACGTCAAGCAAAACGC
TACCAAAGGGATGTCTACTA

Downstream 100 bases:

>100_bases
CAGATTGAATCTAACCCAGCTACTTAGTTTAGATAAAGCGCAACAGACTGAAGCCTTACAAGAAGCCAATGACCTGCTTG
AAAATAAAACCGCCCGTGAA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MSKKLHDNERIKIESNFLRGKLVEGLNDNTTGAFIPDDVQLVKFHGLYQQDHRDYRAERKKQKLEPLYSFLLRARLPGGV
VTPQQLLEIDRIASELTDAGSIRLTTRQTFQYHGIYKRNLKSVFQELKFKANIDSIFTAGDVNRNVICTSNPMQSELHAE
VYDWSTKISEMLLPHSNAFAEIWLDGEKVHETEQEPIYGETMLPRKFKTTVVLPPFNDVDVHANDLNFVAIVENEKIVGF
NVLVGGGLGITHGDKTTFPRKADDFGFIAAEDILEIAKAVVTTQRDLGGRTSRSHARTKYTLETHGVEKFKGEVESRSGI
KFLPSRPYEFTERGDRIGWIKGTDKKWHLTVFIENGRILDYPGKPLKSGLVEIAKVHQGDFRMTANQNLIIASVPESEKE
KIEALARKCGLINDAHSKQRFNSMACVALPTCPLAMAEAERYLPGLVTDVEALLTKHGIGDDHIILRVTGCPNGCARPLL
AEVGLIGKAPGRYNLYLGGNTNGTRLPKMYKENIDEAAIIMTMDELIGRWANERESGECFGDFVIRAAIIAEVIIASKDF
HD

Sequences:

>Translated_562_residues
MSKKLHDNERIKIESNFLRGKLVEGLNDNTTGAFIPDDVQLVKFHGLYQQDHRDYRAERKKQKLEPLYSFLLRARLPGGV
VTPQQLLEIDRIASELTDAGSIRLTTRQTFQYHGIYKRNLKSVFQELKFKANIDSIFTAGDVNRNVICTSNPMQSELHAE
VYDWSTKISEMLLPHSNAFAEIWLDGEKVHETEQEPIYGETMLPRKFKTTVVLPPFNDVDVHANDLNFVAIVENEKIVGF
NVLVGGGLGITHGDKTTFPRKADDFGFIAAEDILEIAKAVVTTQRDLGGRTSRSHARTKYTLETHGVEKFKGEVESRSGI
KFLPSRPYEFTERGDRIGWIKGTDKKWHLTVFIENGRILDYPGKPLKSGLVEIAKVHQGDFRMTANQNLIIASVPESEKE
KIEALARKCGLINDAHSKQRFNSMACVALPTCPLAMAEAERYLPGLVTDVEALLTKHGIGDDHIILRVTGCPNGCARPLL
AEVGLIGKAPGRYNLYLGGNTNGTRLPKMYKENIDEAAIIMTMDELIGRWANERESGECFGDFVIRAAIIAEVIIASKDF
HD
>Mature_561_residues
SKKLHDNERIKIESNFLRGKLVEGLNDNTTGAFIPDDVQLVKFHGLYQQDHRDYRAERKKQKLEPLYSFLLRARLPGGVV
TPQQLLEIDRIASELTDAGSIRLTTRQTFQYHGIYKRNLKSVFQELKFKANIDSIFTAGDVNRNVICTSNPMQSELHAEV
YDWSTKISEMLLPHSNAFAEIWLDGEKVHETEQEPIYGETMLPRKFKTTVVLPPFNDVDVHANDLNFVAIVENEKIVGFN
VLVGGGLGITHGDKTTFPRKADDFGFIAAEDILEIAKAVVTTQRDLGGRTSRSHARTKYTLETHGVEKFKGEVESRSGIK
FLPSRPYEFTERGDRIGWIKGTDKKWHLTVFIENGRILDYPGKPLKSGLVEIAKVHQGDFRMTANQNLIIASVPESEKEK
IEALARKCGLINDAHSKQRFNSMACVALPTCPLAMAEAERYLPGLVTDVEALLTKHGIGDDHIILRVTGCPNGCARPLLA
EVGLIGKAPGRYNLYLGGNTNGTRLPKMYKENIDEAAIIMTMDELIGRWANERESGECFGDFVIRAAIIAEVIIASKDFH
D

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=559, Percent_Identity=64.7584973166368, Blast_Score=768, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=589, Percent_Identity=44.3123938879457, Blast_Score=502, Evalue=1e-143,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_PSYIN (A1T054)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944718.1
- ProteinModelPortal:   A1T054
- SMR:   A1T054
- STRING:   A1T054
- GeneID:   4625589
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3435
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   ESERVFP
- PhylomeDB:   A1T054
- ProtClustDB:   PRK13504
- BioCyc:   PING357804:PING_3435-MONOMER
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 63022; Mature: 62891

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLHDNERIKIESNFLRGKLVEGLNDNTTGAFIPDDVQLVKFHGLYQQDHRDYRAERK
CCCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KQKLEPLYSFLLRARLPGGVVTPQQLLEIDRIASELTDAGSIRLTTRQTFQYHGIYKRNL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHH
KSVFQELKFKANIDSIFTAGDVNRNVICTSNPMQSELHAEVYDWSTKISEMLLPHSNAFA
HHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
EIWLDGEKVHETEQEPIYGETMLPRKFKTTVVLPPFNDVDVHANDLNFVAIVENEKIVGF
EEEECCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCEEEEEEECCEEEEE
NVLVGGGLGITHGDKTTFPRKADDFGFIAAEDILEIAKAVVTTQRDLGGRTSRSHARTKY
EEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
TLETHGVEKFKGEVESRSGIKFLPSRPYEFTERGDRIGWIKGTDKKWHLTVFIENGRILD
EEECCCHHHHHCCCCCCCCCEECCCCCCCHHHCCCEEEEEECCCCEEEEEEEEECCEEEE
YPGKPLKSGLVEIAKVHQGDFRMTANQNLIIASVPESEKEKIEALARKCGLINDAHSKQR
CCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHH
FNSMACVALPTCPLAMAEAERYLPGLVTDVEALLTKHGIGDDHIILRVTGCPNGCARPLL
HCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHH
AEVGLIGKAPGRYNLYLGGNTNGTRLPKMYKENIDEAAIIMTMDELIGRWANERESGECF
HHHCCEECCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCHH
GDFVIRAAIIAEVIIASKDFHD
HHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKKLHDNERIKIESNFLRGKLVEGLNDNTTGAFIPDDVQLVKFHGLYQQDHRDYRAERK
CCCCCCCCEEEEECCHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KQKLEPLYSFLLRARLPGGVVTPQQLLEIDRIASELTDAGSIRLTTRQTFQYHGIYKRNL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCCCHHHHHH
KSVFQELKFKANIDSIFTAGDVNRNVICTSNPMQSELHAEVYDWSTKISEMLLPHSNAFA
HHHHHHHHHHCCCCCEEECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEE
EIWLDGEKVHETEQEPIYGETMLPRKFKTTVVLPPFNDVDVHANDLNFVAIVENEKIVGF
EEEECCHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEECCCEEEEEEECCEEEEE
NVLVGGGLGITHGDKTTFPRKADDFGFIAAEDILEIAKAVVTTQRDLGGRTSRSHARTKY
EEEEECCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
TLETHGVEKFKGEVESRSGIKFLPSRPYEFTERGDRIGWIKGTDKKWHLTVFIENGRILD
EEECCCHHHHHCCCCCCCCCEECCCCCCCHHHCCCEEEEEECCCCEEEEEEEEECCEEEE
YPGKPLKSGLVEIAKVHQGDFRMTANQNLIIASVPESEKEKIEALARKCGLINDAHSKQR
CCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHH
FNSMACVALPTCPLAMAEAERYLPGLVTDVEALLTKHGIGDDHIILRVTGCPNGCARPLL
HCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHH
AEVGLIGKAPGRYNLYLGGNTNGTRLPKMYKENIDEAAIIMTMDELIGRWANERESGECF
HHHCCEECCCCEEEEEECCCCCCCCCCHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCHH
GDFVIRAAIIAEVIIASKDFHD
HHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA