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The map label for this gene is ilvI [H]

Identifier: 119947033

GI number: 119947033

Start: 4241074

End: 4242798

Strand: Direct

Name: ilvI [H]

Synonym: Ping_3430

Alternate gene names: 119947033

Gene position: 4241074-4242798 (Clockwise)

Preceding gene: 119947026

Following gene: 119947034

Centisome position: 93.01

GC content: 40.93

Gene sequence:

>1725_bases
ATGGAGATGTTATCCGGCGCCGAAATGGTTGTCCGATCACTACAAGACGAAGGTATTGAACATATTTTCGGTTACCCTGG
TGGATCAGTATTAGATATTTACGATGCGATTTTTCAATCTGATAAAATTGAACATTATTTAGTACGTCATGAGCAAGCTG
CTGTACATATGGCAGATGCATACTCACGTGCAACCGGTAAAGTGGGTTGTGTACTAGTAACAGCCGGACCCGGTGCAACA
AACTGTATTACAGGTCTTGTAACAGCTTACATGGACTCTATTCCATTAGTTGTGTTATCTGGTCAAGTACCAACCAATTG
GATCGGCGATGATGCTTTCCAGGAAGCAGATATGATCGGCATATCGCGTCCCGTGGTAAAACACAGTTTTTCATGCCGTA
CCGCTGAAGACATTCCAATTGCGATCAAAAAAGCATTTTATATCGCATCAACAGGCCGACCAGGTCCAGTGGTTATCGAT
CTTCCAAAAGACGTACAAAGCCCCCTTCATAAATTTCCTTATGAATACCCTAAATCCGTTGAACTGCGTTCTTATAAACC
AACGCTAAGCGGTCATAAAGGACAAATTAAACGCGCATTAGCGGCATTATTAGGCGCTAAAAAGCCCGTTTTTTATGTCG
GTGGCGGTGCGATTATTGCCAATGCATCAAAACAAGTACTAGAGCTAGCTGAGAAATTAAATTTACCAGTAACTAATACC
TTAATGGGTCTTGGTGCATTTCCTGGTGAACACAAACAATTTATCGGTATGTTAGGCATGCACGGTACTTATGAAGCTAA
CTTATCAATGCATAACGCAGATCTTATCTTCTCTGCCGGTGCTCGTTTTGACGATCGAGTAACCAATAATGTTGATAAAT
TCTGCCCGAATGCAAAAATTATGCAGATAGATATCGACCCAACATCAATTTCTAAAAACATTCATGCGGACCTGCCAATT
GTAGGATCGATTGAAGTTGTACTTCAGCAGATGTTAGACCTACTTGAAGAAACTAAAGCAACGAATGACAGTGAAAGTAT
TAATGCTTGGTGGGATCAAATTAATGAATGGCGTGCTAAAGACTGCCTTGCTTACCAAACATCTGAAACACACATCAAGC
CACAACAAGTGATTCAATGTTTATATAAAGTAACCAAAGGCGATGCTATCGTTACTTCTGACGTTGGTCAGCATCAAATG
GTTGCCGCACAATATTACCCATTTAAAGAACCTCGCCAATGGATTAACTCTGGTGGAGCGGGCACAATGGGCTTTGGTTT
ACCAGCTGCAATGGGTTGTAAGATAGCATTCCCAGATCGCCCTGTTGTATGTGTAACGGGTGATGGCTCTATTCAAATGA
ATATTCAAGAGTTATCAACCTGCATGCAATACAACATTCCTGTTGTTATCTTGCTATTAAACAACCGTTCATTAGGTATG
GTAAAACAGTGGCAAAAAATGTTTTATGACGGTCGCCAATCTCACTCTTATATGGATAGCGTGCCGGATTTCGTTAAATT
ATCTGAAGCATATAACCATATTGGTATCAAAGTAGATAAGATTGAAGAGCTTGAGCCAGCATTAGAACGTGCATTTGAAT
TAAAAGATCGCGTGGTATTTATTGATGTAGCTATAGACCCTGAAGAACATGTTTACCCGATGCAAGTTAAGTTCGGCAGT
ATGCAAGACATGTACCTGAGTCAAACGGAGAGAACCGATGCGTAG

Upstream 100 bases:

>100_bases
AATGCAGGTCATGAATCATGGTCACATGTAATCAAGGTTCACCCTCACCCAATACAGGTGGGAAGTGACGCAATTTACCA
ATTGTATAAAGGAGAAAGTT

Downstream 100 bases:

>100_bases
TATTATTTCAGTATTATTAGAAAATGAACCAGGTTCGCTTGCTCGTGTTGTTGGCTTATTTGCACAACGCGCATATAACA
TCGAGTCATTAACCGTTTCA

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEMLSGAEMVVRSLQDEGIEHIFGYPGGSVLDIYDAIFQSDKIEHYLVRHEQAAVHMADAYSRATGKVGCVLVTAGPGAT
NCITGLVTAYMDSIPLVVLSGQVPTNWIGDDAFQEADMIGISRPVVKHSFSCRTAEDIPIAIKKAFYIASTGRPGPVVID
LPKDVQSPLHKFPYEYPKSVELRSYKPTLSGHKGQIKRALAALLGAKKPVFYVGGGAIIANASKQVLELAEKLNLPVTNT
LMGLGAFPGEHKQFIGMLGMHGTYEANLSMHNADLIFSAGARFDDRVTNNVDKFCPNAKIMQIDIDPTSISKNIHADLPI
VGSIEVVLQQMLDLLEETKATNDSESINAWWDQINEWRAKDCLAYQTSETHIKPQQVIQCLYKVTKGDAIVTSDVGQHQM
VAAQYYPFKEPRQWINSGGAGTMGFGLPAAMGCKIAFPDRPVVCVTGDGSIQMNIQELSTCMQYNIPVVILLLNNRSLGM
VKQWQKMFYDGRQSHSYMDSVPDFVKLSEAYNHIGIKVDKIEELEPALERAFELKDRVVFIDVAIDPEEHVYPMQVKFGS
MQDMYLSQTERTDA

Sequences:

>Translated_574_residues
MEMLSGAEMVVRSLQDEGIEHIFGYPGGSVLDIYDAIFQSDKIEHYLVRHEQAAVHMADAYSRATGKVGCVLVTAGPGAT
NCITGLVTAYMDSIPLVVLSGQVPTNWIGDDAFQEADMIGISRPVVKHSFSCRTAEDIPIAIKKAFYIASTGRPGPVVID
LPKDVQSPLHKFPYEYPKSVELRSYKPTLSGHKGQIKRALAALLGAKKPVFYVGGGAIIANASKQVLELAEKLNLPVTNT
LMGLGAFPGEHKQFIGMLGMHGTYEANLSMHNADLIFSAGARFDDRVTNNVDKFCPNAKIMQIDIDPTSISKNIHADLPI
VGSIEVVLQQMLDLLEETKATNDSESINAWWDQINEWRAKDCLAYQTSETHIKPQQVIQCLYKVTKGDAIVTSDVGQHQM
VAAQYYPFKEPRQWINSGGAGTMGFGLPAAMGCKIAFPDRPVVCVTGDGSIQMNIQELSTCMQYNIPVVILLLNNRSLGM
VKQWQKMFYDGRQSHSYMDSVPDFVKLSEAYNHIGIKVDKIEELEPALERAFELKDRVVFIDVAIDPEEHVYPMQVKFGS
MQDMYLSQTERTDA
>Mature_574_residues
MEMLSGAEMVVRSLQDEGIEHIFGYPGGSVLDIYDAIFQSDKIEHYLVRHEQAAVHMADAYSRATGKVGCVLVTAGPGAT
NCITGLVTAYMDSIPLVVLSGQVPTNWIGDDAFQEADMIGISRPVVKHSFSCRTAEDIPIAIKKAFYIASTGRPGPVVID
LPKDVQSPLHKFPYEYPKSVELRSYKPTLSGHKGQIKRALAALLGAKKPVFYVGGGAIIANASKQVLELAEKLNLPVTNT
LMGLGAFPGEHKQFIGMLGMHGTYEANLSMHNADLIFSAGARFDDRVTNNVDKFCPNAKIMQIDIDPTSISKNIHADLPI
VGSIEVVLQQMLDLLEETKATNDSESINAWWDQINEWRAKDCLAYQTSETHIKPQQVIQCLYKVTKGDAIVTSDVGQHQM
VAAQYYPFKEPRQWINSGGAGTMGFGLPAAMGCKIAFPDRPVVCVTGDGSIQMNIQELSTCMQYNIPVVILLLNNRSLGM
VKQWQKMFYDGRQSHSYMDSVPDFVKLSEAYNHIGIKVDKIEELEPALERAFELKDRVVFIDVAIDPEEHVYPMQVKFGS
MQDMYLSQTERTDA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=27.7777777777778, Blast_Score=199, Evalue=8e-51,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=27.5929549902153, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=65.6794425087108, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=554, Percent_Identity=41.5162454873646, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=546, Percent_Identity=34.0659340659341, Blast_Score=318, Evalue=5e-88,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=28.9285714285714, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=25.2236135957066, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=566, Percent_Identity=27.5618374558304, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17531301, Length=566, Percent_Identity=27.5618374558304, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17542570, Length=519, Percent_Identity=26.5895953757225, Blast_Score=156, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=41.9520547945205, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.113475177305, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922626, Length=580, Percent_Identity=27.4137931034483, Blast_Score=200, Evalue=2e-51,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63421; Mature: 63421

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLQDEGIEHIFGYPGGSVLDIYDAIFQSDKIEHYLVRHEQAAVHMADA
CCCCCHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YSRATGKVGCVLVTAGPGATNCITGLVTAYMDSIPLVVLSGQVPTNWIGDDAFQEADMIG
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHC
ISRPVVKHSFSCRTAEDIPIAIKKAFYIASTGRPGPVVIDLPKDVQSPLHKFPYEYPKSV
CCCHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCC
ELRSYKPTLSGHKGQIKRALAALLGAKKPVFYVGGGAIIANASKQVLELAEKLNLPVTNT
EEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHCCCCHHHH
LMGLGAFPGEHKQFIGMLGMHGTYEANLSMHNADLIFSAGARFDDRVTNNVDKFCPNAKI
HHHCCCCCCCHHHHHHHHCCCCEEECCEEEECCCEEEECCCCCCHHHHCCHHHHCCCCEE
MQIDIDPTSISKNIHADLPIVGSIEVVLQQMLDLLEETKATNDSESINAWWDQINEWRAK
EEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DCLAYQTSETHIKPQQVIQCLYKVTKGDAIVTSDVGQHQMVAAQYYPFKEPRQWINSGGA
HHEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCHHHHHHHHCCCC
GTMGFGLPAAMGCKIAFPDRPVVCVTGDGSIQMNIQELSTCMQYNIPVVILLLNNRSLGM
CCCCCCCHHHCCCEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCH
VKQWQKMFYDGRQSHSYMDSVPDFVKLSEAYNHIGIKVDKIEELEPALERAFELKDRVVF
HHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEE
IDVAIDPEEHVYPMQVKFGSMQDMYLSQTERTDA
EEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLQDEGIEHIFGYPGGSVLDIYDAIFQSDKIEHYLVRHEQAAVHMADA
CCCCCHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
YSRATGKVGCVLVTAGPGATNCITGLVTAYMDSIPLVVLSGQVPTNWIGDDAFQEADMIG
HHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHC
ISRPVVKHSFSCRTAEDIPIAIKKAFYIASTGRPGPVVIDLPKDVQSPLHKFPYEYPKSV
CCCHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEECCHHHHHHHHHCCCCCCCCC
ELRSYKPTLSGHKGQIKRALAALLGAKKPVFYVGGGAIIANASKQVLELAEKLNLPVTNT
EEECCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEEEECCHHHHHHHHHHCCCCHHHH
LMGLGAFPGEHKQFIGMLGMHGTYEANLSMHNADLIFSAGARFDDRVTNNVDKFCPNAKI
HHHCCCCCCCHHHHHHHHCCCCEEECCEEEECCCEEEECCCCCCHHHHCCHHHHCCCCEE
MQIDIDPTSISKNIHADLPIVGSIEVVLQQMLDLLEETKATNDSESINAWWDQINEWRAK
EEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
DCLAYQTSETHIKPQQVIQCLYKVTKGDAIVTSDVGQHQMVAAQYYPFKEPRQWINSGGA
HHEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCEEEECCCCCHHHHHHHHCCCC
GTMGFGLPAAMGCKIAFPDRPVVCVTGDGSIQMNIQELSTCMQYNIPVVILLLNNRSLGM
CCCCCCCHHHCCCEEECCCCCEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCH
VKQWQKMFYDGRQSHSYMDSVPDFVKLSEAYNHIGIKVDKIEELEPALERAFELKDRVVF
HHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCEEE
IDVAIDPEEHVYPMQVKFGSMQDMYLSQTERTDA
EEEEECCCCCEEEEEEECCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]