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The map label for this gene is proS

Identifier: 119946895

GI number: 119946895

Start: 4059526

End: 4061241

Strand: Direct

Name: proS

Synonym: Ping_3289

Alternate gene names: 119946895

Gene position: 4059526-4061241 (Clockwise)

Preceding gene: 119946893

Following gene: 119946896

Centisome position: 89.03

GC content: 43.82

Gene sequence:

>1716_bases
ATGCGCACGACCAACTACTTACTTTCAACTCAAAAAGAAGCACCCAGCGATGCGGTAATTGTAAGCCATCAGCTTATGTT
ACGTGCAGGCATGATCCGTAAATTAGCATCGGGTTTATATACCTGGTTACCGACGGGGTTACGGGTTTTACGTAAAGTTG
AAACTATCGTGCGTGAAGAGATGGAACGAGCCGGAGGTGTTGAAGTCTTAATGCCAATTGTGCAACCTGCAGATCTTTGG
CTAGAAACAGGTCGCTGGGATCAATACGGCGGTGAGCTGTTACGTATTAAAGATCGCCATACACGTGATTTTGTATTGGG
CCCAACACACGAGGAAGTGATCACTGAGCTTGTGCGCAAAGAAGTAAACAGTTACAAACAGCTACCACTCAATCTTTTCC
AAATTCAGACAAAATTCCGCGATGAAACTCGTCCGCGTTTTGGTGTTATGCGTGCGCGCGAATTTACCATGAAAGATGCG
TACTCTTTTCATATGGATCAAGCTTGTCTGGAAAAAACATATCAGAAAATGTTTGACGCTTATTGTCGTATCTTTGACCG
CTTAGGTCTTGAATACCGCCCTGTTATTGCCGATACTGGAAGTATTGGCGGCAGTGCATCTCATGAATTCCACGTACTTG
CGCAAAGCGGTGAAGATGCCATTGTTTTCTCAACTGAAAGTGATTACGCGGCAAATATTGAAAAAGCAGAAGCATTGGCA
CCGAGCACTGTTCTTGCAGCACCCACTGCTGAAATGGGACTGCTTGATACCCCAAATGCAAAAACTATCGCTGAACTCGT
TTCGAAACATGGCATTGCTATTGAAAAGACGGTCAAAACTTTATTTGTTAAAGCGTCTGATCAAATTGAAACGGAGCTGA
TCGCGCTTATCATCCGTGGTGATCACGAACTTAATGAGATTAAAGCTGAAAATTTAGCCTCTGTCGCTGCTCCACTTGAA
TTCGCCAGCGAAAGTGAAATCAGAGCATTGGTTAATGCAGGCCCGGGCTCACTGGGTCCAGTTTCTCTACCAGTGCCCTT
TATTGTTGACCGTTCAGTGGCTGTTATGAGCGACTTTAGTGCCGGTGCAAACATTGATAATAAACATTACTGCAACATTA
ACTGGGGCCGCGACGTTGAACTTGCTCAAGTTGAAGATCTGCGTAATGTCGTTGAAGGTGATCCAAGCCCCTGCGGTAGT
GGAACACTTTCAATGGCACGGGGTATCGAAGTGGGACATATTTTCCAACTGGGTGATACTTATACTAAAGCGATGAATGC
CGGCGTACTTAACCAGCAAGGTAAAAACCAGATATTAACCATGGGTTGTTACGGCATTGGTATCTCACGTATTGTTGCTG
CTGCTATTGAGCAAAACAATGATAAAAATGGCATTATTTGGAACAATACACTGGCGCCTTTTTCGGTAGTTATTGTCCCA
ATGAATATGCATAAATCACATCGTGTCGCAGAACTTGCTGAAAAATACTATGCAGAATTACAAGCAGCAGGTATCGAGGT
GTTATTTGACGATCGTAAAGAACGCCCTGGTATTATGTTTGCGGATGCAGAGCTTATGGGTATTCCACATACCCTGGTGA
TTGGAGATCGTAGTTTGGATAATGGCGTGATTGAATATAAAGATCGCCTATCGGGTGTAAAACAGGAAGTTGCTATTGAT
GAGGTTATTAACTTTATCAAAGCCCAACTCGCTTAA

Upstream 100 bases:

>100_bases
TAAAATTCAATTATATTTTCTAAATAGATGAAGAAACCTTTAGTCATTACGTTTCCACTGCTAAAATCAGTGCTGTTTAA
TTTATCAATTAGGGATAACT

Downstream 100 bases:

>100_bases
TCATTCGCGTATCCTACCATTAACAAAGTTAGTATTTTTTATTCCGGCGTGATCTTTGAAGAAAATATAAAGGATCTTGC
CGTCCCCAGTCTTAGCTAAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTTNYLLSTQKEAPSDAVIVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVETIVREEMERAGGVEVLMPIVQPADLW
LETGRWDQYGGELLRIKDRHTRDFVLGPTHEEVITELVRKEVNSYKQLPLNLFQIQTKFRDETRPRFGVMRAREFTMKDA
YSFHMDQACLEKTYQKMFDAYCRIFDRLGLEYRPVIADTGSIGGSASHEFHVLAQSGEDAIVFSTESDYAANIEKAEALA
PSTVLAAPTAEMGLLDTPNAKTIAELVSKHGIAIEKTVKTLFVKASDQIETELIALIIRGDHELNEIKAENLASVAAPLE
FASESEIRALVNAGPGSLGPVSLPVPFIVDRSVAVMSDFSAGANIDNKHYCNINWGRDVELAQVEDLRNVVEGDPSPCGS
GTLSMARGIEVGHIFQLGDTYTKAMNAGVLNQQGKNQILTMGCYGIGISRIVAAAIEQNNDKNGIIWNNTLAPFSVVIVP
MNMHKSHRVAELAEKYYAELQAAGIEVLFDDRKERPGIMFADAELMGIPHTLVIGDRSLDNGVIEYKDRLSGVKQEVAID
EVINFIKAQLA

Sequences:

>Translated_571_residues
MRTTNYLLSTQKEAPSDAVIVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVETIVREEMERAGGVEVLMPIVQPADLW
LETGRWDQYGGELLRIKDRHTRDFVLGPTHEEVITELVRKEVNSYKQLPLNLFQIQTKFRDETRPRFGVMRAREFTMKDA
YSFHMDQACLEKTYQKMFDAYCRIFDRLGLEYRPVIADTGSIGGSASHEFHVLAQSGEDAIVFSTESDYAANIEKAEALA
PSTVLAAPTAEMGLLDTPNAKTIAELVSKHGIAIEKTVKTLFVKASDQIETELIALIIRGDHELNEIKAENLASVAAPLE
FASESEIRALVNAGPGSLGPVSLPVPFIVDRSVAVMSDFSAGANIDNKHYCNINWGRDVELAQVEDLRNVVEGDPSPCGS
GTLSMARGIEVGHIFQLGDTYTKAMNAGVLNQQGKNQILTMGCYGIGISRIVAAAIEQNNDKNGIIWNNTLAPFSVVIVP
MNMHKSHRVAELAEKYYAELQAAGIEVLFDDRKERPGIMFADAELMGIPHTLVIGDRSLDNGVIEYKDRLSGVKQEVAID
EVINFIKAQLA
>Mature_571_residues
MRTTNYLLSTQKEAPSDAVIVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVETIVREEMERAGGVEVLMPIVQPADLW
LETGRWDQYGGELLRIKDRHTRDFVLGPTHEEVITELVRKEVNSYKQLPLNLFQIQTKFRDETRPRFGVMRAREFTMKDA
YSFHMDQACLEKTYQKMFDAYCRIFDRLGLEYRPVIADTGSIGGSASHEFHVLAQSGEDAIVFSTESDYAANIEKAEALA
PSTVLAAPTAEMGLLDTPNAKTIAELVSKHGIAIEKTVKTLFVKASDQIETELIALIIRGDHELNEIKAENLASVAAPLE
FASESEIRALVNAGPGSLGPVSLPVPFIVDRSVAVMSDFSAGANIDNKHYCNINWGRDVELAQVEDLRNVVEGDPSPCGS
GTLSMARGIEVGHIFQLGDTYTKAMNAGVLNQQGKNQILTMGCYGIGISRIVAAAIEQNNDKNGIIWNNTLAPFSVVIVP
MNMHKSHRVAELAEKYYAELQAAGIEVLFDDRKERPGIMFADAELMGIPHTLVIGDRSLDNGVIEYKDRLSGVKQEVAID
EVINFIKAQLA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=229, Percent_Identity=44.9781659388646, Blast_Score=218, Evalue=1e-56,
Organism=Escherichia coli, GI1786392, Length=570, Percent_Identity=68.0701754385965, Blast_Score=825, Evalue=0.0,
Organism=Escherichia coli, GI1788013, Length=159, Percent_Identity=27.0440251572327, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115532348, Length=244, Percent_Identity=37.7049180327869, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI193203271, Length=86, Percent_Identity=36.046511627907, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320931, Length=534, Percent_Identity=29.0262172284644, Blast_Score=239, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24656200, Length=238, Percent_Identity=39.0756302521008, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PSYIN (A1SZR1)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944575.1
- ProteinModelPortal:   A1SZR1
- SMR:   A1SZR1
- STRING:   A1SZR1
- GeneID:   4625775
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3289
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   A1SZR1
- ProtClustDB:   PRK09194
- BioCyc:   PING357804:PING_3289-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63136; Mature: 63136

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50862 AA_TRNA_LIGASE_II; PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTTNYLLSTQKEAPSDAVIVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVETIVREE
CCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERAGGVEVLMPIVQPADLWLETGRWDQYGGELLRIKDRHTRDFVLGPTHEEVITELVRK
HHHHCCCEEEHHHHCCHHHHEECCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLFQIQTKFRDETRPRFGVMRAREFTMKDAYSFHMDQACLEKTYQKMFDA
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YCRIFDRLGLEYRPVIADTGSIGGSASHEFHVLAQSGEDAIVFSTESDYAANIEKAEALA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHCC
PSTVLAAPTAEMGLLDTPNAKTIAELVSKHGIAIEKTVKTLFVKASDQIETELIALIIRG
CCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCHHHHEEEEEEEEC
DHELNEIKAENLASVAAPLEFASESEIRALVNAGPGSLGPVSLPVPFIVDRSVAVMSDFS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHCCC
AGANIDNKHYCNINWGRDVELAQVEDLRNVVEGDPSPCGSGTLSMARGIEVGHIFQLGDT
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCHH
YTKAMNAGVLNQQGKNQILTMGCYGIGISRIVAAAIEQNNDKNGIIWNNTLAPFSVVIVP
HHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEE
MNMHKSHRVAELAEKYYAELQAAGIEVLFDDRKERPGIMFADAELMGIPHTLVIGDRSLD
CCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCEEEEEECCCCC
NGVIEYKDRLSGVKQEVAIDEVINFIKAQLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTTNYLLSTQKEAPSDAVIVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVETIVREE
CCCCCCHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERAGGVEVLMPIVQPADLWLETGRWDQYGGELLRIKDRHTRDFVLGPTHEEVITELVRK
HHHHCCCEEEHHHHCCHHHHEECCCCCCCCCCEEEEECCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLFQIQTKFRDETRPRFGVMRAREFTMKDAYSFHMDQACLEKTYQKMFDA
HHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YCRIFDRLGLEYRPVIADTGSIGGSASHEFHVLAQSGEDAIVFSTESDYAANIEKAEALA
HHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCEEEEECCCCCCCCCHHHHHCC
PSTVLAAPTAEMGLLDTPNAKTIAELVSKHGIAIEKTVKTLFVKASDQIETELIALIIRG
CCCEEECCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEECCCHHHHEEEEEEEEC
DHELNEIKAENLASVAAPLEFASESEIRALVNAGPGSLGPVSLPVPFIVDRSVAVMSDFS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHHHCCC
AGANIDNKHYCNINWGRDVELAQVEDLRNVVEGDPSPCGSGTLSMARGIEVGHIFQLGDT
CCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCHH
YTKAMNAGVLNQQGKNQILTMGCYGIGISRIVAAAIEQNNDKNGIIWNNTLAPFSVVIVP
HHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEE
MNMHKSHRVAELAEKYYAELQAAGIEVLFDDRKERPGIMFADAELMGIPHTLVIGDRSLD
CCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEECCHHCCCCEEEEEECCCCC
NGVIEYKDRLSGVKQEVAIDEVINFIKAQLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA