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The map label for this gene is dacA [H]

Identifier: 119946648

GI number: 119946648

Start: 3725236

End: 3726456

Strand: Direct

Name: dacA [H]

Synonym: Ping_3027

Alternate gene names: 119946648

Gene position: 3725236-3726456 (Clockwise)

Preceding gene: 119946625

Following gene: 119946649

Centisome position: 81.7

GC content: 39.97

Gene sequence:

>1221_bases
ATGATAAGAAAATATTTTTATTCTAATCATTCACAGGTATCTTTCATGAAAAAAATAAGCCTTCTGGTTACCGCGCTCAT
TTTCAGCTTTAACCTATTTGCAAACCCAACAATAACGCCAAGTGCACCGGAAATTGGCGCAAAGGGATATCTGCTTATTG
ATTTTAATTCAGGAAAAGTACTTTCTGAAAATAATAGTGATGTAAGACTTAATCCAGCCAGCCTTACAAAAATGATGACC
AGTTATATCATTGGCCAGGAAATTAAATCCGGCAACTTATCACCCGACGATAAAGTGACTATTTCCGAAAAGGCGTGGTC
AAAAAATTTCCCTGAGTCTTCTAAGATGTTTATTGAAGTGGGTAAGGAAGTGAGCGTCGCCGACTTAAATCGTGGCATTA
TCATACAATCAGGTAATGATGCCTGTGTGGCGATGGCTGAATATATCGCCGGTAGTGAAGGTGCCTTTGCAGATTTAATG
AATTCTTGGGCAGCCCAGTTAGGAATGAATAATACGCAATTTAAAAATAGCCACGGTTTAACAGCTGAGGGTCACTACAC
AACACCGCATGATATGGCTATCTTGGCGCAAGCATTAATTAATGATGTGCCCGATGAATACCTTATATACAGTGAAAAAT
CATTTAAATTTAATGGTATTGATCAACCAAATCGTAACGCTTTATTATGGGACAAAAGTTTAGACGTAGATGGTATCAAA
ACAGGCCATACCAACGAGGCAGGTTACAGCTTGATTTCTTCGGCAACCAAAGATGGCATGCGTCTAATCAGTGTAGTAAT
GGGCACCCGTTCAACTTCTGCACGTCTAACCGAAAGTAAAAAATTACTCAATTGGGGATTCCGCTTTTTTGAAACGCTGA
CTCCTTACAATGCTGGCGACCAATTAGCGAATGAAAGAATTTGGATGGGTAGCCAGCCAATGATTCGATTAGGCGTAAAA
GATGATACGCCTATTACGCTGCCAAGAGGTGAGGCCAAAAATTTAACCGCTAATTTTATTATTGAAAATGAATTGCGCGC
CCCCATCCAAAAAGGTGATGTGGTCGGCAAGGTAAACTATTCAGTGACGGGTCAACAGACAGTGGATGACGAACCGATTG
CGACCTACGATCTGATCGCCTTAGAAACCGTGGAAGAAGGTGGTTTATTTAGTCGCTTGATAGATTATATTAAGTTATTA
TTTATTGGTTGGTTTAGCTGA

Upstream 100 bases:

>100_bases
ATTAATAAAATGAATAGGTGAATGTGACAGCCTGATTTTAAAGCATCCTTGATCTGTAGAAATCACTCTTCACCCCTTAG
CAAATAAAGGTTGATCTGCT

Downstream 100 bases:

>100_bases
TTTTAGCCCCACATTATAATAATAAGTGAGCTTTCTCCTGAGCGAGTTCGCCATAGAACATAAAAAATTCGATTAACATT
GCCCCTGGAGTTTAAAATGG

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MIRKYFYSNHSQVSFMKKISLLVTALIFSFNLFANPTITPSAPEIGAKGYLLIDFNSGKVLSENNSDVRLNPASLTKMMT
SYIIGQEIKSGNLSPDDKVTISEKAWSKNFPESSKMFIEVGKEVSVADLNRGIIIQSGNDACVAMAEYIAGSEGAFADLM
NSWAAQLGMNNTQFKNSHGLTAEGHYTTPHDMAILAQALINDVPDEYLIYSEKSFKFNGIDQPNRNALLWDKSLDVDGIK
TGHTNEAGYSLISSATKDGMRLISVVMGTRSTSARLTESKKLLNWGFRFFETLTPYNAGDQLANERIWMGSQPMIRLGVK
DDTPITLPRGEAKNLTANFIIENELRAPIQKGDVVGKVNYSVTGQQTVDDEPIATYDLIALETVEEGGLFSRLIDYIKLL
FIGWFS

Sequences:

>Translated_406_residues
MIRKYFYSNHSQVSFMKKISLLVTALIFSFNLFANPTITPSAPEIGAKGYLLIDFNSGKVLSENNSDVRLNPASLTKMMT
SYIIGQEIKSGNLSPDDKVTISEKAWSKNFPESSKMFIEVGKEVSVADLNRGIIIQSGNDACVAMAEYIAGSEGAFADLM
NSWAAQLGMNNTQFKNSHGLTAEGHYTTPHDMAILAQALINDVPDEYLIYSEKSFKFNGIDQPNRNALLWDKSLDVDGIK
TGHTNEAGYSLISSATKDGMRLISVVMGTRSTSARLTESKKLLNWGFRFFETLTPYNAGDQLANERIWMGSQPMIRLGVK
DDTPITLPRGEAKNLTANFIIENELRAPIQKGDVVGKVNYSVTGQQTVDDEPIATYDLIALETVEEGGLFSRLIDYIKLL
FIGWFS
>Mature_406_residues
MIRKYFYSNHSQVSFMKKISLLVTALIFSFNLFANPTITPSAPEIGAKGYLLIDFNSGKVLSENNSDVRLNPASLTKMMT
SYIIGQEIKSGNLSPDDKVTISEKAWSKNFPESSKMFIEVGKEVSVADLNRGIIIQSGNDACVAMAEYIAGSEGAFADLM
NSWAAQLGMNNTQFKNSHGLTAEGHYTTPHDMAILAQALINDVPDEYLIYSEKSFKFNGIDQPNRNALLWDKSLDVDGIK
TGHTNEAGYSLISSATKDGMRLISVVMGTRSTSARLTESKKLLNWGFRFFETLTPYNAGDQLANERIWMGSQPMIRLGVK
DDTPITLPRGEAKNLTANFIIENELRAPIQKGDVVGKVNYSVTGQQTVDDEPIATYDLIALETVEEGGLFSRLIDYIKLL
FIGWFS

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=400, Percent_Identity=52, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1787062, Length=371, Percent_Identity=49.0566037735849, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI87082022, Length=388, Percent_Identity=43.298969072165, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI87082054, Length=257, Percent_Identity=26.4591439688716, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44885; Mature: 44885

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKYFYSNHSQVSFMKKISLLVTALIFSFNLFANPTITPSAPEIGAKGYLLIDFNSGKV
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEE
LSENNSDVRLNPASLTKMMTSYIIGQEIKSGNLSPDDKVTISEKAWSKNFPESSKMFIEV
EECCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCCEEE
GKEVSVADLNRGIIIQSGNDACVAMAEYIAGSEGAFADLMNSWAAQLGMNNTQFKNSHGL
CCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TAEGHYTTPHDMAILAQALINDVPDEYLIYSEKSFKFNGIDQPNRNALLWDKSLDVDGIK
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEEEECCCCCCCCC
TGHTNEAGYSLISSATKDGMRLISVVMGTRSTSARLTESKKLLNWGFRFFETLTPYNAGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QLANERIWMGSQPMIRLGVKDDTPITLPRGEAKNLTANFIIENELRAPIQKGDVVGKVNY
HHHCCEEEECCCCEEEEECCCCCCEECCCCCCCCCEEEEEEECCHHCCHHCCCEEEEEEE
SVTGQQTVDDEPIATYDLIALETVEEGGLFSRLIDYIKLLFIGWFS
EECCCCCCCCCCCCEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRKYFYSNHSQVSFMKKISLLVTALIFSFNLFANPTITPSAPEIGAKGYLLIDFNSGKV
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCEE
LSENNSDVRLNPASLTKMMTSYIIGQEIKSGNLSPDDKVTISEKAWSKNFPESSKMFIEV
EECCCCCEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHCCCCCCCCCCCEEE
GKEVSVADLNRGIIIQSGNDACVAMAEYIAGSEGAFADLMNSWAAQLGMNNTQFKNSHGL
CCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
TAEGHYTTPHDMAILAQALINDVPDEYLIYSEKSFKFNGIDQPNRNALLWDKSLDVDGIK
CCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCEEEEECCCCCCCCC
TGHTNEAGYSLISSATKDGMRLISVVMGTRSTSARLTESKKLLNWGFRFFETLTPYNAGD
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QLANERIWMGSQPMIRLGVKDDTPITLPRGEAKNLTANFIIENELRAPIQKGDVVGKVNY
HHHCCEEEECCCCEEEEECCCCCCEECCCCCCCCCEEEEEEECCHHCCHHCCCEEEEEEE
SVTGQQTVDDEPIATYDLIALETVEEGGLFSRLIDYIKLLFIGWFS
EECCCCCCCCCCCCEEEEEEEEEHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]