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The map label for this gene is braD [H]

Identifier: 119946608

GI number: 119946608

Start: 3685152

End: 3686795

Strand: Direct

Name: braD [H]

Synonym: Ping_2987

Alternate gene names: 119946608

Gene position: 3685152-3686795 (Clockwise)

Preceding gene: 119946607

Following gene: 119946609

Centisome position: 80.82

GC content: 44.95

Gene sequence:

>1644_bases
ATGTTACGAATATTTTCCCTGTTGGTTTTCTCCTGCATGTCGTGGTTTGCCCTTGCCGAAAATGCGCCGGCAACAGAGAG
CAACACTCAATTCGAAACACTGCTTGCGCAGTTACCGTCAACCAAATTATCCAAAATGGCGCCTTTAGTAGATAAAATCG
CAGCGCAACCATCACCGCAAGTACCGGAACTGCTGCAGCACTTAATGGAGGGCGAGCTGTATTACCTGAAAGCCGCTAAC
GAGCTTGTCTATGCTCAGCCAAACCAGCAACAAACCTATGATTTAACGTCTGTTTTAAAGACCGAAGCATTAGCACCCGT
CAGTATTTATGCACTCAAAAAAATCAGAACTAATAACCGTATCCGCGGGCAAATTCGTGATTTGCTGGCCAATATGTATT
TATCCAATAGCTCTCCTGCCGTTCGCCTGCAGGCTATTAAGCAGCTTTTAGATAACCCGAGTAAAACCGGCATAACCAGC
ATTACGCGATTAATAAAAACAGAGCAGGAAAAAGAGATACGCGAACTGATGTCCGTGGTGCTGCTGATTGAGCAGTTAGA
GAAAGGTCAGACGGCTGAAAAACTCGCCGCTATTAGCCAGTTAGAAGGCAGATTGGAACCGACGGTTAAAAATGCACTGG
TAAAAGAGCTTAAAAAATTATCTGCTTCACAAGACTTATCTATTTCACAAGATAAGAGCGAACTTCATAAAGCACTCACC
CGTGCTATTGGTCAAATGGAACAGGATGGTGATTTCTACCAGCTGCTTGATTATCTCTTCTTTGGCTTAAGCATGGGCTC
GGTTTTATTACTGGCAGCCATCGGTTTGACTATTACCTTTGGAGTCATGGGCGTGATTAATATGGCCCACGGTGAAATGA
TGATGCTTGGTGCCTATACCACTTATGTGATTCAGCAAACCTTTCCAAATTTAATAGAGTATTCACTGCTGATGGCGGTG
CCCGCTGCCTTTTTGGTGTCAGGTTTAGTCGGTGTGTTAATTGAACGCGGCGTGATCCGTTTTCTAAAAGGACGCCCGTT
AGAAACGCTGTTGGCTACTTTTGGTATCAGCCTTATTTTACAGCAATTAGTAAGAACGGTTTTCTCACCGCTCAACCGTC
AAGTGGAAGCGCCACAGTGGATGAGCGGCTCACTGGAAATTAATCCTATTTTTTCATTAACCTATAACCGTTTATATATC
ATTATATTCGCGCTGATGGTGTTTTTCTCTTTATTAATGATTTTGAAAAAATCATCGCTGGGACTGCATGTGCGGGCCGT
TTCACAAAACCGCGATATGGCACGCGCGCTAAGCATAAAAAGCTCATGGGTTGATGCCATCACCTTTGGCTTAGGATCAG
GCGTTGCGGGCATCGCCGGTGTGGCATTAAGCCAGTTAACCAATGTTGGCCCTAACCTCGGACAGGACTATATTATTGAT
TCATTTTTGGTGGTGGTATTTGGTGGTGTCGGTAGCCTATGGGGCACATTAGTGGCCGCAATGACGCTGGGCACCTTTAA
CAAATTCTTAGAGCCGTCGATGGGGCCTGTTCTGGCTAATATCATCGTCCTGGTCGGGTTAGTCTTGTTTATTCAAAAAC
GACCTAAGGGCTTGTTTCCTCAAAAAGGGAGGTCTGCTGAATAA

Upstream 100 bases:

>100_bases
AATAAACCTCAGTTATAGTGAATATAAAGTGATTATCAGAGAGGTAACAATACCTCTCTTTTTTATAAGAAGTAGATTAT
TTAACATTAAGGAAAAGCTA

Downstream 100 bases:

>100_bases
TGAAAAATTTACTTCATTCACCTGGTTTTAAAAAACTTAACAGTTTACATTTTGTGATTGCGATTTTATTTATCACAACC
CTGTATGTGACAGTGTGTAA

Product: ABC transporter inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLRIFSLLVFSCMSWFALAENAPATESNTQFETLLAQLPSTKLSKMAPLVDKIAAQPSPQVPELLQHLMEGELYYLKAAN
ELVYAQPNQQQTYDLTSVLKTEALAPVSIYALKKIRTNNRIRGQIRDLLANMYLSNSSPAVRLQAIKQLLDNPSKTGITS
ITRLIKTEQEKEIRELMSVVLLIEQLEKGQTAEKLAAISQLEGRLEPTVKNALVKELKKLSASQDLSISQDKSELHKALT
RAIGQMEQDGDFYQLLDYLFFGLSMGSVLLLAAIGLTITFGVMGVINMAHGEMMMLGAYTTYVIQQTFPNLIEYSLLMAV
PAAFLVSGLVGVLIERGVIRFLKGRPLETLLATFGISLILQQLVRTVFSPLNRQVEAPQWMSGSLEINPIFSLTYNRLYI
IIFALMVFFSLLMILKKSSLGLHVRAVSQNRDMARALSIKSSWVDAITFGLGSGVAGIAGVALSQLTNVGPNLGQDYIID
SFLVVVFGGVGSLWGTLVAAMTLGTFNKFLEPSMGPVLANIIVLVGLVLFIQKRPKGLFPQKGRSAE

Sequences:

>Translated_547_residues
MLRIFSLLVFSCMSWFALAENAPATESNTQFETLLAQLPSTKLSKMAPLVDKIAAQPSPQVPELLQHLMEGELYYLKAAN
ELVYAQPNQQQTYDLTSVLKTEALAPVSIYALKKIRTNNRIRGQIRDLLANMYLSNSSPAVRLQAIKQLLDNPSKTGITS
ITRLIKTEQEKEIRELMSVVLLIEQLEKGQTAEKLAAISQLEGRLEPTVKNALVKELKKLSASQDLSISQDKSELHKALT
RAIGQMEQDGDFYQLLDYLFFGLSMGSVLLLAAIGLTITFGVMGVINMAHGEMMMLGAYTTYVIQQTFPNLIEYSLLMAV
PAAFLVSGLVGVLIERGVIRFLKGRPLETLLATFGISLILQQLVRTVFSPLNRQVEAPQWMSGSLEINPIFSLTYNRLYI
IIFALMVFFSLLMILKKSSLGLHVRAVSQNRDMARALSIKSSWVDAITFGLGSGVAGIAGVALSQLTNVGPNLGQDYIID
SFLVVVFGGVGSLWGTLVAAMTLGTFNKFLEPSMGPVLANIIVLVGLVLFIQKRPKGLFPQKGRSAE
>Mature_547_residues
MLRIFSLLVFSCMSWFALAENAPATESNTQFETLLAQLPSTKLSKMAPLVDKIAAQPSPQVPELLQHLMEGELYYLKAAN
ELVYAQPNQQQTYDLTSVLKTEALAPVSIYALKKIRTNNRIRGQIRDLLANMYLSNSSPAVRLQAIKQLLDNPSKTGITS
ITRLIKTEQEKEIRELMSVVLLIEQLEKGQTAEKLAAISQLEGRLEPTVKNALVKELKKLSASQDLSISQDKSELHKALT
RAIGQMEQDGDFYQLLDYLFFGLSMGSVLLLAAIGLTITFGVMGVINMAHGEMMMLGAYTTYVIQQTFPNLIEYSLLMAV
PAAFLVSGLVGVLIERGVIRFLKGRPLETLLATFGISLILQQLVRTVFSPLNRQVEAPQWMSGSLEINPIFSLTYNRLYI
IIFALMVFFSLLMILKKSSLGLHVRAVSQNRDMARALSIKSSWVDAITFGLGSGVAGIAGVALSQLTNVGPNLGQDYIID
SFLVVVFGGVGSLWGTLVAAMTLGTFNKFLEPSMGPVLANIIVLVGLVLFIQKRPKGLFPQKGRSAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=309, Percent_Identity=28.4789644012945, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 60111; Mature: 60111

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIFSLLVFSCMSWFALAENAPATESNTQFETLLAQLPSTKLSKMAPLVDKIAAQPSPQ
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
VPELLQHLMEGELYYLKAANELVYAQPNQQQTYDLTSVLKTEALAPVSIYALKKIRTNNR
HHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCH
IRGQIRDLLANMYLSNSSPAVRLQAIKQLLDNPSKTGITSITRLIKTEQEKEIRELMSVV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLIEQLEKGQTAEKLAAISQLEGRLEPTVKNALVKELKKLSASQDLSISQDKSELHKALT
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RAIGQMEQDGDFYQLLDYLFFGLSMGSVLLLAAIGLTITFGVMGVINMAHGEMMMLGAYT
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
TYVIQQTFPNLIEYSLLMAVPAAFLVSGLVGVLIERGVIRFLKGRPLETLLATFGISLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QQLVRTVFSPLNRQVEAPQWMSGSLEINPIFSLTYNRLYIIIFALMVFFSLLMILKKSSL
HHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLHVRAVSQNRDMARALSIKSSWVDAITFGLGSGVAGIAGVALSQLTNVGPNLGQDYIID
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SFLVVVFGGVGSLWGTLVAAMTLGTFNKFLEPSMGPVLANIIVLVGLVLFIQKRPKGLFP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QKGRSAE
CCCCCCC
>Mature Secondary Structure
MLRIFSLLVFSCMSWFALAENAPATESNTQFETLLAQLPSTKLSKMAPLVDKIAAQPSPQ
CHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
VPELLQHLMEGELYYLKAANELVYAQPNQQQTYDLTSVLKTEALAPVSIYALKKIRTNNR
HHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCH
IRGQIRDLLANMYLSNSSPAVRLQAIKQLLDNPSKTGITSITRLIKTEQEKEIRELMSVV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLIEQLEKGQTAEKLAAISQLEGRLEPTVKNALVKELKKLSASQDLSISQDKSELHKALT
HHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
RAIGQMEQDGDFYQLLDYLFFGLSMGSVLLLAAIGLTITFGVMGVINMAHGEMMMLGAYT
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHH
TYVIQQTFPNLIEYSLLMAVPAAFLVSGLVGVLIERGVIRFLKGRPLETLLATFGISLIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
QQLVRTVFSPLNRQVEAPQWMSGSLEINPIFSLTYNRLYIIIFALMVFFSLLMILKKSSL
HHHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GLHVRAVSQNRDMARALSIKSSWVDAITFGLGSGVAGIAGVALSQLTNVGPNLGQDYIID
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
SFLVVVFGGVGSLWGTLVAAMTLGTFNKFLEPSMGPVLANIIVLVGLVLFIQKRPKGLFP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
QKGRSAE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]