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The map label for this gene is proB [H]

Identifier: 119946574

GI number: 119946574

Start: 3640310

End: 3641446

Strand: Reverse

Name: proB [H]

Synonym: Ping_2953

Alternate gene names: 119946574

Gene position: 3641446-3640310 (Counterclockwise)

Preceding gene: 119946575

Following gene: 119946573

Centisome position: 79.86

GC content: 42.66

Gene sequence:

>1137_bases
ATGAACTTCTTGATAAACGAAACCAGCATGAAAACCAGTAAAAAAACCATTGTCGTAAAATTAGGAACAAGTGTGCTGAC
CAGCGGCACGACAAAGTTAGATCGCGCACAGATGGTTGAAATTGTACGTCAATGTGCGCTATTGCACCATCAGGGTCATC
ATATTATTGTGGTAACCTCGGGCGCTATTGCTGCTGGTAAAGAGCATTTAGGGTTGCATAATATTGAGCCTACGATAGCA
AATAAACAGATGCTTGCGGCAGTTGGGCAGGGGCAATTAGTGCGTGAGTGGGAAAATCTGTTCAAAATATACGGGTTACA
TGTTGGACAAATGTTATTAACCCGTGCTGATTTAGATGATCGCCTGCGTTTTTTGAATGCGCGAGATACGATAAAAACAC
TGTTAAAATACCGAATTATTCCTTTAGTTAATGAAAATGATGCAGTCGCTACCAGTGAAATTAAAGTGGGTGATAATGAT
AATCTGTCGGCATTAGTGGCTTTATTAGCCGATGCTGATCAGTTACTATTATTGACCGATCAACTGGGTCTGTTTACCGG
TGATCCGCGTAATAACCCCGATGCGCAGCTAATCCCCGAAATCCAAAAAATCACTCAGGAAATTCACGAGCTGGCAAGTG
GCAGTATAAGCGGCCTGGGTACGGGAGGCATGGCGACGAAAATTGAATCAGCAATGATTGCCAATAGAGCGGGCATTGAA
GTGGTCATAGCATCGGGTCATAAAAAAGATGTGATTATCAAGGCCGCTAATAATCAGTCGGTAGGAACGCGTTTCAGCTC
CGAAAATACCCGTTTAGAAGCGCGTAAAAACTGGATTTTTGCCGCGTCGCGCAGCAGTGGTAAAATTATTGTTGATGCGG
GGGCGGAGAAAGCCATTGTACATAACGGCAGCAGTTTGTTAGCAAAAGGCATTGTAGCAACAACGGAAAATTTTTCGCGC
GGTTATGTTGTTAGTATCGTTAATCAGGCCGGCCAAGAGTTAGCCCGAGGGCTGGTACGTTATAAAAAAGTCGATATAGA
AAAAATAAAAGGGTTACACTCAGAACAAATTGAAGCAGTATTAGGTTTTGAACATGGTAGAGTCGTTATACATCGAGATG
ATTTAGTACTCTTATAA

Upstream 100 bases:

>100_bases
ATAAGATCAGCGCTATTGAAAACGCAGATTTACTATATTAAATTGAATAATGTTATAGTCACAAACAGTAACGAGTCGCT
TGTTGCTGTTTTTTTGGTTA

Downstream 100 bases:

>100_bases
ACGATAATTAATACTTAATTAAGGAAAAGTTCATGGATTTACAATCATTAGGTAAATTAGCAAAAGAAGCAAGTTATGAA
CTTGCAATTACTGGCAGCGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MNFLINETSMKTSKKTIVVKLGTSVLTSGTTKLDRAQMVEIVRQCALLHHQGHHIIVVTSGAIAAGKEHLGLHNIEPTIA
NKQMLAAVGQGQLVREWENLFKIYGLHVGQMLLTRADLDDRLRFLNARDTIKTLLKYRIIPLVNENDAVATSEIKVGDND
NLSALVALLADADQLLLLTDQLGLFTGDPRNNPDAQLIPEIQKITQEIHELASGSISGLGTGGMATKIESAMIANRAGIE
VVIASGHKKDVIIKAANNQSVGTRFSSENTRLEARKNWIFAASRSSGKIIVDAGAEKAIVHNGSSLLAKGIVATTENFSR
GYVVSIVNQAGQELARGLVRYKKVDIEKIKGLHSEQIEAVLGFEHGRVVIHRDDLVLL

Sequences:

>Translated_378_residues
MNFLINETSMKTSKKTIVVKLGTSVLTSGTTKLDRAQMVEIVRQCALLHHQGHHIIVVTSGAIAAGKEHLGLHNIEPTIA
NKQMLAAVGQGQLVREWENLFKIYGLHVGQMLLTRADLDDRLRFLNARDTIKTLLKYRIIPLVNENDAVATSEIKVGDND
NLSALVALLADADQLLLLTDQLGLFTGDPRNNPDAQLIPEIQKITQEIHELASGSISGLGTGGMATKIESAMIANRAGIE
VVIASGHKKDVIIKAANNQSVGTRFSSENTRLEARKNWIFAASRSSGKIIVDAGAEKAIVHNGSSLLAKGIVATTENFSR
GYVVSIVNQAGQELARGLVRYKKVDIEKIKGLHSEQIEAVLGFEHGRVVIHRDDLVLL
>Mature_378_residues
MNFLINETSMKTSKKTIVVKLGTSVLTSGTTKLDRAQMVEIVRQCALLHHQGHHIIVVTSGAIAAGKEHLGLHNIEPTIA
NKQMLAAVGQGQLVREWENLFKIYGLHVGQMLLTRADLDDRLRFLNARDTIKTLLKYRIIPLVNENDAVATSEIKVGDND
NLSALVALLADADQLLLLTDQLGLFTGDPRNNPDAQLIPEIQKITQEIHELASGSISGLGTGGMATKIESAMIANRAGIE
VVIASGHKKDVIIKAANNQSVGTRFSSENTRLEARKNWIFAASRSSGKIIVDAGAEKAIVHNGSSLLAKGIVATTENFSR
GYVVSIVNQAGQELARGLVRYKKVDIEKIKGLHSEQIEAVLGFEHGRVVIHRDDLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=285, Percent_Identity=34.3859649122807, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI21361368, Length=287, Percent_Identity=34.1463414634146, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=58.0821917808219, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17569967, Length=280, Percent_Identity=32.8571428571429, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17569969, Length=280, Percent_Identity=32.8571428571429, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6321822, Length=421, Percent_Identity=33.9667458432304, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320506, Length=418, Percent_Identity=33.7320574162679, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21357643, Length=299, Percent_Identity=29.7658862876254, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 41041; Mature: 41041

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFLINETSMKTSKKTIVVKLGTSVLTSGTTKLDRAQMVEIVRQCALLHHQGHHIIVVTS
CCEEECCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GAIAAGKEHLGLHNIEPTIANKQMLAAVGQGQLVREWENLFKIYGLHVGQMLLTRADLDD
CCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RLRFLNARDTIKTLLKYRIIPLVNENDAVATSEIKVGDNDNLSALVALLADADQLLLLTD
HHHHHCHHHHHHHHHHHEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEC
QLGLFTGDPRNNPDAQLIPEIQKITQEIHELASGSISGLGTGGMATKIESAMIANRAGIE
CCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VVIASGHKKDVIIKAANNQSVGTRFSSENTRLEARKNWIFAASRSSGKIIVDAGAEKAIV
EEEECCCCCEEEEEECCCCCCCCEECCCCCEEEECCCEEEEEECCCCEEEEECCCCEEEE
HNGSSLLAKGIVATTENFSRGYVVSIVNQAGQELARGLVRYKKVDIEKIKGLHSEQIEAV
ECCHHHHHHCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHH
LGFEHGRVVIHRDDLVLL
CCCCCCEEEEEECCEEEC
>Mature Secondary Structure
MNFLINETSMKTSKKTIVVKLGTSVLTSGTTKLDRAQMVEIVRQCALLHHQGHHIIVVTS
CCEEECCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GAIAAGKEHLGLHNIEPTIANKQMLAAVGQGQLVREWENLFKIYGLHVGQMLLTRADLDD
CCCCCCHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
RLRFLNARDTIKTLLKYRIIPLVNENDAVATSEIKVGDNDNLSALVALLADADQLLLLTD
HHHHHCHHHHHHHHHHHEEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEC
QLGLFTGDPRNNPDAQLIPEIQKITQEIHELASGSISGLGTGGMATKIESAMIANRAGIE
CCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VVIASGHKKDVIIKAANNQSVGTRFSSENTRLEARKNWIFAASRSSGKIIVDAGAEKAIV
EEEECCCCCEEEEEECCCCCCCCEECCCCCEEEECCCEEEEEECCCCEEEEECCCCEEEE
HNGSSLLAKGIVATTENFSRGYVVSIVNQAGQELARGLVRYKKVDIEKIKGLHSEQIEAV
ECCHHHHHHCCEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHH
LGFEHGRVVIHRDDLVLL
CCCCCCEEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]