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The map label for this gene is ampD [H]

Identifier: 119946561

GI number: 119946561

Start: 3625393

End: 3625938

Strand: Reverse

Name: ampD [H]

Synonym: Ping_2940

Alternate gene names: 119946561

Gene position: 3625938-3625393 (Counterclockwise)

Preceding gene: 119946562

Following gene: 119946560

Centisome position: 79.52

GC content: 42.49

Gene sequence:

>546_bases
GTGATTAATAAACTCGGCTTTTATCAAAATGCCAGACAGATATCCAGTCCCTTTTATAATCAACGACCTGCAGGCAGCGA
GATTAGCTTATTGGTTATTCATTGTATTAGTCTGCCCGAAGGTTGTTATGGCGGCCCGGAAATTGAGTCTTTATTTACCG
GATGTTTAGATTGTTATGCTCACCCTGATTTTAGCAGCCTTGAAGGGCTTGAAGTCTCTGCACATTGTGTGATTCGCCGT
AATGGTGAGATTGAACAATATGTGCCTTTTGAAAAAGGTGCCTGGCATGCCGGGGCATCCTATTTTGCCGGAGTTGATCG
ATGCAATGATTATTCCATTGGTATTGAGCTTGAGGGCACAGATAAAAGCGCCTATACAGAAAGACAATATCGTTCACTTG
CAACCTTGACTCAAAAAATACAGCAACGTTATCCTGCTATCAGTAAACAACGAATAGTCGCACATAGTGAGATAGCGCCG
GGCAGAAAAACGGATCCCGGCAGTGGATTTGACTGGGTTTATTATTTGAGTTTGTTAAGTGATTGA

Upstream 100 bases:

>100_bases
CAGCCGTCAGCCGTCAGCCGTCAGCCGTCAGCTCGCAGCCTTCTAGCTTTAACGCCTTTCAGCATTCTGGCCTTTTCGCC
TTTTTTAAGGAGCATTATTT

Downstream 100 bases:

>100_bases
ACCCTTGTCTTAGTATTATCTTCTCTGGCGCTGAGGCGCTAATATATGAGTCTGCCAACCTACCCCATATTATACTTTGG
TTATTGCCGCACCTTATTAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MINKLGFYQNARQISSPFYNQRPAGSEISLLVIHCISLPEGCYGGPEIESLFTGCLDCYAHPDFSSLEGLEVSAHCVIRR
NGEIEQYVPFEKGAWHAGASYFAGVDRCNDYSIGIELEGTDKSAYTERQYRSLATLTQKIQQRYPAISKQRIVAHSEIAP
GRKTDPGSGFDWVYYLSLLSD

Sequences:

>Translated_181_residues
MINKLGFYQNARQISSPFYNQRPAGSEISLLVIHCISLPEGCYGGPEIESLFTGCLDCYAHPDFSSLEGLEVSAHCVIRR
NGEIEQYVPFEKGAWHAGASYFAGVDRCNDYSIGIELEGTDKSAYTERQYRSLATLTQKIQQRYPAISKQRIVAHSEIAP
GRKTDPGSGFDWVYYLSLLSD
>Mature_181_residues
MINKLGFYQNARQISSPFYNQRPAGSEISLLVIHCISLPEGCYGGPEIESLFTGCLDCYAHPDFSSLEGLEVSAHCVIRR
NGEIEQYVPFEKGAWHAGASYFAGVDRCNDYSIGIELEGTDKSAYTERQYRSLATLTQKIQQRYPAISKQRIVAHSEIAP
GRKTDPGSGFDWVYYLSLLSD

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=180, Percent_Identity=53.8888888888889, Blast_Score=201, Evalue=2e-53,
Organism=Escherichia coli, GI1787092, Length=125, Percent_Identity=41.6, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20216; Mature: 20216

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKLGFYQNARQISSPFYNQRPAGSEISLLVIHCISLPEGCYGGPEIESLFTGCLDCYA
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHC
HPDFSSLEGLEVSAHCVIRRNGEIEQYVPFEKGAWHAGASYFAGVDRCNDYSIGIELEGT
CCCCCCCCCCEEEEEEEEEECCCHHEECCCCCCCCCCCCHHHHHCCCCCCEEEEEEEECC
DKSAYTERQYRSLATLTQKIQQRYPAISKQRIVAHSEIAPGRKTDPGSGFDWVYYLSLLS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
D
C
>Mature Secondary Structure
MINKLGFYQNARQISSPFYNQRPAGSEISLLVIHCISLPEGCYGGPEIESLFTGCLDCYA
CCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHC
HPDFSSLEGLEVSAHCVIRRNGEIEQYVPFEKGAWHAGASYFAGVDRCNDYSIGIELEGT
CCCCCCCCCCEEEEEEEEEECCCHHEECCCCCCCCCCCCHHHHHCCCCCCEEEEEEEECC
DKSAYTERQYRSLATLTQKIQQRYPAISKQRIVAHSEIAPGRKTDPGSGFDWVYYLSLLS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]