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The map label for this gene is gatA [H]

Identifier: 119946560

GI number: 119946560

Start: 3623599

End: 3624834

Strand: Reverse

Name: gatA [H]

Synonym: Ping_2939

Alternate gene names: 119946560

Gene position: 3624834-3623599 (Counterclockwise)

Preceding gene: 119946561

Following gene: 119946559

Centisome position: 79.5

GC content: 40.78

Gene sequence:

>1236_bases
ATGGATACAAGTGTTTATTGTCGACAAGGGCCTAAAAGCATTGCTGCAATCAAGACACGTGGCGTACTCTCAGGTTATCG
GTTTGTGTTTAAGGATGTTTTTGATGTACAAGGGTATGTGACAGGTGCGGGAAATCCAACCTGGTTAATGAGTCATGAGC
CAGCGCTTCACACTTCTCCTATTATTACACAATTATTAATACAGGGAGCACATTGCTCGGGTCGAGTGCAATCCTCTGAA
TTAGCTTGTGGTTTTGATGGTATGAATACCCATTATGGTACTGCTATTAATCACTTAGCACCTAAATGCCAGTCTGGCGG
TTCATCAAGCTGCTCAGCAGCTGTTGCTCGTGGTGATGGTGATTTTGCTTTAGGAATTGATGCTGCTGGTGAAATAAGAA
TATCTGCAAGTTACTGCGGGCTTTTTGGATTAAGGGCAACTTATGCCGCATTGCCGCTTGATCATAGCTTTTCGCTGGCA
AAAAGCTTTGATACAGCAGGCCTTCTTACGCGTAACCTCACTGTAATGAGCAATGTTGTTACCAGTTTATTCCGGGAGCA
ACCACCCGTGGATCAATTTGAGGATGTTTTTATTTTTGTGGAAAAAAGAGCCTTAGAATTGATGGATTCGGAAGTGAAAG
AGGAATTAACTAACAAGTTAAATAGACTGGCGCGTAATCGTTATGTTGTCTCAACTGATTCGTTACTTGATATTTGCGAG
GTCAGTTTAGAGGAGTTAGCGGAGTGGTTCAGCATTATTCAAGGGTATGAATTGATTCAAGAGCATAGTGAGTGGTTGGC
AGAACATCAAAGTGCTGTTACTAAGGATGTCTCCAGACGGTTTGATAAATCAAAAAATATAAGCATCGATGAGTACGTTG
AAGCGAAACAAAAAATGGCTAATTTTAGCACTTTGTTTATTTATTTATTAAAGAGCAAAGGAGGCTGGTTATGTCTTCCA
ACAACACCTGGGTTGCCACCGGAGCTGAAACAATCTGTTAATTCATTAATTTATTATCGTAAAAGGTTATTGGGCCTGAC
CGCGTTAGCCAGTTTATGCGGTTTACCCCAGCTGCATTTACCCTTAGGTAAAATCAATGGAAAATCCTATGGTCTATCCT
TGATTGGCATGCCTAATAGTGAAAAAAAATTGATAAAACAGGGGCAATATCTGCTGCAAAGCCTGGCTGTCAATGAGTCT
GTTAATAGCAAGAGTTGGTTAAATAACAAACTCTGA

Upstream 100 bases:

>100_bases
GCAAGACCCTATAAACATTTGTGTCGAGCGAGCAATTTCTATTTTTTATCAAAGACGCAAATGACAGTCTTAAATTTCCA
ACTATCAGGTAAAATCAATT

Downstream 100 bases:

>100_bases
GCGAATCAGCGAGGGATATACGTCATAATCAGCAGTCTTTATTAGGTAAAATTAAGCAACAACTTTAATATACTTATTTA
AATAAGGTTGTGAATAAGGT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MDTSVYCRQGPKSIAAIKTRGVLSGYRFVFKDVFDVQGYVTGAGNPTWLMSHEPALHTSPIITQLLIQGAHCSGRVQSSE
LACGFDGMNTHYGTAINHLAPKCQSGGSSSCSAAVARGDGDFALGIDAAGEIRISASYCGLFGLRATYAALPLDHSFSLA
KSFDTAGLLTRNLTVMSNVVTSLFREQPPVDQFEDVFIFVEKRALELMDSEVKEELTNKLNRLARNRYVVSTDSLLDICE
VSLEELAEWFSIIQGYELIQEHSEWLAEHQSAVTKDVSRRFDKSKNISIDEYVEAKQKMANFSTLFIYLLKSKGGWLCLP
TTPGLPPELKQSVNSLIYYRKRLLGLTALASLCGLPQLHLPLGKINGKSYGLSLIGMPNSEKKLIKQGQYLLQSLAVNES
VNSKSWLNNKL

Sequences:

>Translated_411_residues
MDTSVYCRQGPKSIAAIKTRGVLSGYRFVFKDVFDVQGYVTGAGNPTWLMSHEPALHTSPIITQLLIQGAHCSGRVQSSE
LACGFDGMNTHYGTAINHLAPKCQSGGSSSCSAAVARGDGDFALGIDAAGEIRISASYCGLFGLRATYAALPLDHSFSLA
KSFDTAGLLTRNLTVMSNVVTSLFREQPPVDQFEDVFIFVEKRALELMDSEVKEELTNKLNRLARNRYVVSTDSLLDICE
VSLEELAEWFSIIQGYELIQEHSEWLAEHQSAVTKDVSRRFDKSKNISIDEYVEAKQKMANFSTLFIYLLKSKGGWLCLP
TTPGLPPELKQSVNSLIYYRKRLLGLTALASLCGLPQLHLPLGKINGKSYGLSLIGMPNSEKKLIKQGQYLLQSLAVNES
VNSKSWLNNKL
>Mature_411_residues
MDTSVYCRQGPKSIAAIKTRGVLSGYRFVFKDVFDVQGYVTGAGNPTWLMSHEPALHTSPIITQLLIQGAHCSGRVQSSE
LACGFDGMNTHYGTAINHLAPKCQSGGSSSCSAAVARGDGDFALGIDAAGEIRISASYCGLFGLRATYAALPLDHSFSLA
KSFDTAGLLTRNLTVMSNVVTSLFREQPPVDQFEDVFIFVEKRALELMDSEVKEELTNKLNRLARNRYVVSTDSLLDICE
VSLEELAEWFSIIQGYELIQEHSEWLAEHQSAVTKDVSRRFDKSKNISIDEYVEAKQKMANFSTLFIYLLKSKGGWLCLP
TTPGLPPELKQSVNSLIYYRKRLLGLTALASLCGLPQLHLPLGKINGKSYGLSLIGMPNSEKKLIKQGQYLLQSLAVNES
VNSKSWLNNKL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6319685, Length=406, Percent_Identity=23.3990147783251, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6323950, Length=399, Percent_Identity=25.5639097744361, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 45235; Mature: 45235

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTSVYCRQGPKSIAAIKTRGVLSGYRFVFKDVFDVQGYVTGAGNPTWLMSHEPALHTSP
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHH
IITQLLIQGAHCSGRVQSSELACGFDGMNTHYGTAINHLAPKCQSGGSSSCSAAVARGDG
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEECCCC
DFALGIDAAGEIRISASYCGLFGLRATYAALPLDHSFSLAKSFDTAGLLTRNLTVMSNVV
CEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
TSLFREQPPVDQFEDVFIFVEKRALELMDSEVKEELTNKLNRLARNRYVVSTDSLLDICE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHH
VSLEELAEWFSIIQGYELIQEHSEWLAEHQSAVTKDVSRRFDKSKNISIDEYVEAKQKMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NFSTLFIYLLKSKGGWLCLPTTPGLPPELKQSVNSLIYYRKRLLGLTALASLCGLPQLHL
HHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PLGKINGKSYGLSLIGMPNSEKKLIKQGQYLLQSLAVNESVNSKSWLNNKL
CHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
>Mature Secondary Structure
MDTSVYCRQGPKSIAAIKTRGVLSGYRFVFKDVFDVQGYVTGAGNPTWLMSHEPALHTSP
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCEEEECCCCCCCCHH
IITQLLIQGAHCSGRVQSSELACGFDGMNTHYGTAINHLAPKCQSGGSSSCSAAVARGDG
HHHHHHHHCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHEECCCC
DFALGIDAAGEIRISASYCGLFGLRATYAALPLDHSFSLAKSFDTAGLLTRNLTVMSNVV
CEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
TSLFREQPPVDQFEDVFIFVEKRALELMDSEVKEELTNKLNRLARNRYVVSTDSLLDICE
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHH
VSLEELAEWFSIIQGYELIQEHSEWLAEHQSAVTKDVSRRFDKSKNISIDEYVEAKQKMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
NFSTLFIYLLKSKGGWLCLPTTPGLPPELKQSVNSLIYYRKRLLGLTALASLCGLPQLHL
HHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEC
PLGKINGKSYGLSLIGMPNSEKKLIKQGQYLLQSLAVNESVNSKSWLNNKL
CHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA