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The map label for this gene is SucB [H]

Identifier: 119946547

GI number: 119946547

Start: 3608602

End: 3610233

Strand: Reverse

Name: SucB [H]

Synonym: Ping_2926

Alternate gene names: 119946547

Gene position: 3610233-3608602 (Counterclockwise)

Preceding gene: 119946548

Following gene: 119946546

Centisome position: 79.18

GC content: 45.71

Gene sequence:

>1632_bases
ATGTCTGAATTAAAAGAATTTTTACTGCCTGATATTGGTGCCGATGCGGCTGATATTACTGATATTTTAGTATCTGTTGG
TGACACTATCGCCGTGGAGCAAGATGTATTAACCATTGAAGGTGACAAAGCCTCGATGGATGTACCCTCATCGGTTGCCG
GTGTGGTTAAAGAAATTAAAGTGAAAGTAGGTGACAGTGTTTCTGAAGGTAACCTAGTACTGATGGTTGAAGTTGAAGTT
GCTGATGCTGATGCGCCTGCTGCCGATGCACCTGCTGCTGAGGCACCTGCTTCACCTGTGGAAGAAGCGAGCCCTGCGGT
TGAAGCGCCTGCTGCTGCAACGACTCAATTAAAAGAAATATCTGTTCCCGATATCGGTGGTGACGAAGTTGAAGTGACGG
CAATCCTTGTTTCTGTTGGAGATAGCATAGCGGAAGAGCAAGATATCCTAACGGTTGAAGGCGATAAAGCGTCAATGGAT
GTCCCTGCACCCTTTGCGGGTGTAGTTAAAGAAATTAAAGCCGCTGTGGGTGATAAAGTTTCTGAAGGTTCATTAATTTT
AGTGGTTGAAGTACAAGGTGCTGCTCCTGCTCCTGCTCCCGCTGCTGCTGAACCGGCTCCTACTCCTGCTGAACCCGCTC
CTGCAGCCGCTGCGCCTGTTGCTGCCGCTGCTGAAGCACCAAAAGCTGCTGCTCAACTTAGCCCCTCACAGGTTTCTGTT
GCCGGCTCAATTAAAGCTTCTCCTTCGGTGCGCCGTACTGCGCGTGAGTTTAATTTGGATCTTTCGGTTATTCCAGCAAC
GGGTATTAAAGGTCGTACGACCAAAGAAGACGTACAGACTTATGTTAAAGCACAGCTATTGCTGGCTAAATCTGGCGGCG
GTGGTGGCTTGCAAGTGCTTGCTTCTCCAAAAGTTGATTTCGCTAAATTTGGCGAAGTGGAAGTTAAGCCACTTTCACGT
ATCCAAAAAATATCAGGTCCGACTCTACACCGTAACTGGGTAACTATCCCGCATGTTACACAATTTGATGAAGTCGATAT
CACTGAACTTGAAGCGTTCCGTAAAGAACAAAATGCGATTGCAGTTAAGCGCGACTTAGGTCTGAAAATCAGTCCGTTAG
TCTTTATGATGAAAGCAGTGGCTAAAGCACTGCAACAATATCCTGATTTTAACTCTTCATTATCAGCCGATGGTGAAAGT
TTAATCCTGAAAAAATACATCAATATCGGTATTGCGGTCGATACACCAAACGGTCTGGTTGTCCCCGTAGTAAAAGATGT
CATAAACAAAGGGATCTATGATCTGTCGCGTGAACTCGGAGAAATTTCGAAAAAAGCGCGTGCCGGTAAATTAACCACCA
GAGATATGCAGGGTGGCAGTATGACTATCTCCAGTCTTGGTGGTATTGGCGGTACACAGTTTACCCCTATCGTAAATGCG
CCTGAAGTTGCTATTTTAGGTGTTTCTAAATCGGCAATGAAACCATTATGGAACGGTAAAGAGTTTGAGCCTCGTCTGAT
GGTTCCACTTGCACTTTCCTATGATCACCGTGTCATCGATGGTGCAGAGGGTGCTCGCTTCATCACTGCGATTAATAATT
ACTTGTCTGACTTACGGACATTAATCTTATAA

Upstream 100 bases:

>100_bases
ATTTGCTATCGATGTAAACAAAACCAACCCATTATTTGCGTAAATTAAATGCGCTGTTATTAAACAATAACAGCGTCTTT
TATTGATTAGGAAAATTATT

Downstream 100 bases:

>100_bases
TTATGTTAACAGGTTGGCATTTGTCGACCTTTTATTTTGCATTTATTTGACAATAAAATAGACTCGCAAAACTTCAATCG
TTTTTCTGGCGTAACGGCTA

Product: 2-oxoglutarate dehydrogenase complex, dihydrolipoamide acetyltransferase E2 component

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MSELKEFLLPDIGADAADITDILVSVGDTIAVEQDVLTIEGDKASMDVPSSVAGVVKEIKVKVGDSVSEGNLVLMVEVEV
ADADAPAADAPAAEAPASPVEEASPAVEAPAAATTQLKEISVPDIGGDEVEVTAILVSVGDSIAEEQDILTVEGDKASMD
VPAPFAGVVKEIKAAVGDKVSEGSLILVVEVQGAAPAPAPAAAEPAPTPAEPAPAAAAPVAAAAEAPKAAAQLSPSQVSV
AGSIKASPSVRRTAREFNLDLSVIPATGIKGRTTKEDVQTYVKAQLLLAKSGGGGGLQVLASPKVDFAKFGEVEVKPLSR
IQKISGPTLHRNWVTIPHVTQFDEVDITELEAFRKEQNAIAVKRDLGLKISPLVFMMKAVAKALQQYPDFNSSLSADGES
LILKKYINIGIAVDTPNGLVVPVVKDVINKGIYDLSRELGEISKKARAGKLTTRDMQGGSMTISSLGGIGGTQFTPIVNA
PEVAILGVSKSAMKPLWNGKEFEPRLMVPLALSYDHRVIDGAEGARFITAINNYLSDLRTLIL

Sequences:

>Translated_543_residues
MSELKEFLLPDIGADAADITDILVSVGDTIAVEQDVLTIEGDKASMDVPSSVAGVVKEIKVKVGDSVSEGNLVLMVEVEV
ADADAPAADAPAAEAPASPVEEASPAVEAPAAATTQLKEISVPDIGGDEVEVTAILVSVGDSIAEEQDILTVEGDKASMD
VPAPFAGVVKEIKAAVGDKVSEGSLILVVEVQGAAPAPAPAAAEPAPTPAEPAPAAAAPVAAAAEAPKAAAQLSPSQVSV
AGSIKASPSVRRTAREFNLDLSVIPATGIKGRTTKEDVQTYVKAQLLLAKSGGGGGLQVLASPKVDFAKFGEVEVKPLSR
IQKISGPTLHRNWVTIPHVTQFDEVDITELEAFRKEQNAIAVKRDLGLKISPLVFMMKAVAKALQQYPDFNSSLSADGES
LILKKYINIGIAVDTPNGLVVPVVKDVINKGIYDLSRELGEISKKARAGKLTTRDMQGGSMTISSLGGIGGTQFTPIVNA
PEVAILGVSKSAMKPLWNGKEFEPRLMVPLALSYDHRVIDGAEGARFITAINNYLSDLRTLIL
>Mature_542_residues
SELKEFLLPDIGADAADITDILVSVGDTIAVEQDVLTIEGDKASMDVPSSVAGVVKEIKVKVGDSVSEGNLVLMVEVEVA
DADAPAADAPAAEAPASPVEEASPAVEAPAAATTQLKEISVPDIGGDEVEVTAILVSVGDSIAEEQDILTVEGDKASMDV
PAPFAGVVKEIKAAVGDKVSEGSLILVVEVQGAAPAPAPAAAEPAPTPAEPAPAAAAPVAAAAEAPKAAAQLSPSQVSVA
GSIKASPSVRRTAREFNLDLSVIPATGIKGRTTKEDVQTYVKAQLLLAKSGGGGGLQVLASPKVDFAKFGEVEVKPLSRI
QKISGPTLHRNWVTIPHVTQFDEVDITELEAFRKEQNAIAVKRDLGLKISPLVFMMKAVAKALQQYPDFNSSLSADGESL
ILKKYINIGIAVDTPNGLVVPVVKDVINKGIYDLSRELGEISKKARAGKLTTRDMQGGSMTISSLGGIGGTQFTPIVNAP
EVAILGVSKSAMKPLWNGKEFEPRLMVPLALSYDHRVIDGAEGARFITAINNYLSDLRTLIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=472, Percent_Identity=29.4491525423729, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI31711992, Length=341, Percent_Identity=32.5513196480938, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI19923748, Length=257, Percent_Identity=34.2412451361868, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI203098816, Length=440, Percent_Identity=27.5, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI203098753, Length=440, Percent_Identity=27.5, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=35.2201257861635, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=539, Percent_Identity=58.9981447124304, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=32.0987654320988, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=28.7735849056604, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=417, Percent_Identity=32.1342925659472, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=427, Percent_Identity=30.2107728337237, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=227, Percent_Identity=32.5991189427313, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=408, Percent_Identity=29.4117647058824, Blast_Score=153, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=28.8372093023256, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=433, Percent_Identity=30.9468822170901, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24645909, Length=237, Percent_Identity=35.0210970464135, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=28.8793103448276, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=28.8793103448276, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56416; Mature: 56285

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELKEFLLPDIGADAADITDILVSVGDTIAVEQDVLTIEGDKASMDVPSSVAGVVKEIK
CCHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHH
VKVGDSVSEGNLVLMVEVEVADADAPAADAPAAEAPASPVEEASPAVEAPAAATTQLKEI
HHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCC
SVPDIGGDEVEVTAILVSVGDSIAEEQDILTVEGDKASMDVPAPFAGVVKEIKAAVGDKV
CCCCCCCCCEEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
SEGSLILVVEVQGAAPAPAPAAAEPAPTPAEPAPAAAAPVAAAAEAPKAAAQLSPSQVSV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHCCCCCEEE
AGSIKASPSVRRTAREFNLDLSVIPATGIKGRTTKEDVQTYVKAQLLLAKSGGGGGLQVL
EECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEE
ASPKVDFAKFGEVEVKPLSRIQKISGPTLHRNWVTIPHVTQFDEVDITELEAFRKEQNAI
ECCCCCHHHCCCEEEHHHHHHHHHCCCCEECCEEECCCCCCCCCCCHHHHHHHHHHCCCE
AVKRDLGLKISPLVFMMKAVAKALQQYPDFNSSLSADGESLILKKYINIGIAVDTPNGLV
EEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCEEEEEEECCCCEE
VPVVKDVINKGIYDLSRELGEISKKARAGKLTTRDMQGGSMTISSLGGIGGTQFTPIVNA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHCCCCCCCCCCCCCC
PEVAILGVSKSAMKPLWNGKEFEPRLMVPLALSYDHRVIDGAEGARFITAINNYLSDLRT
CCEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHH
LIL
HHC
>Mature Secondary Structure 
SELKEFLLPDIGADAADITDILVSVGDTIAVEQDVLTIEGDKASMDVPSSVAGVVKEIK
CHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCEEEEECCCCCCCCCHHHHHHHHHHH
VKVGDSVSEGNLVLMVEVEVADADAPAADAPAAEAPASPVEEASPAVEAPAAATTQLKEI
HHCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHCC
SVPDIGGDEVEVTAILVSVGDSIAEEQDILTVEGDKASMDVPAPFAGVVKEIKAAVGDKV
CCCCCCCCCEEEEEEEEECCCHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCC
SEGSLILVVEVQGAAPAPAPAAAEPAPTPAEPAPAAAAPVAAAAEAPKAAAQLSPSQVSV
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHCCCCCEEE
AGSIKASPSVRRTAREFNLDLSVIPATGIKGRTTKEDVQTYVKAQLLLAKSGGGGGLQVL
EECCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEE
ASPKVDFAKFGEVEVKPLSRIQKISGPTLHRNWVTIPHVTQFDEVDITELEAFRKEQNAI
ECCCCCHHHCCCEEEHHHHHHHHHCCCCEECCEEECCCCCCCCCCCHHHHHHHHHHCCCE
AVKRDLGLKISPLVFMMKAVAKALQQYPDFNSSLSADGESLILKKYINIGIAVDTPNGLV
EEECCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCEEEEEEECCCCEE
VPVVKDVINKGIYDLSRELGEISKKARAGKLTTRDMQGGSMTISSLGGIGGTQFTPIVNA
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEHHHHCCCCCCCCCCCCCC
PEVAILGVSKSAMKPLWNGKEFEPRLMVPLALSYDHRVIDGAEGARFITAINNYLSDLRT
CCEEEEECCHHHHCCCCCCCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHH
LIL
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]