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The map label for this gene is acnB [H]

Identifier: 119946522

GI number: 119946522

Start: 3576977

End: 3579577

Strand: Reverse

Name: acnB [H]

Synonym: Ping_2899

Alternate gene names: 119946522

Gene position: 3579577-3576977 (Counterclockwise)

Preceding gene: 119946523

Following gene: 119946521

Centisome position: 78.51

GC content: 42.75

Gene sequence:

>2601_bases
GTGCTAGAAGCTTATCGTAAACATATCGCTGAACGTGCCGAGCAAGGCATAGTCCCACAACCTTTGAATGCTGAACAAAC
TGCGGCATTAGTTGAATTGTTAAAAACTCCACCACAGGGCGAAGAAGCCTTTTTGGTTGATCTTTTAGAAAACCGTATTC
CTCCTGGTGTTGATGAAGCTGCCTATGTTAAAGCTGGGTTTTTGTCTGCCATTGCATCTGCTGAAACCATTTCTCCCATT
ATCTCTCGTGAGCATGCTGTTAAGCTGTTGGGTACGATGCAGGGTGGTTACAATATTCAACCGTTGATTAGCCAACTTGA
TAATGAAAAGCTTGCGCCGTTAGCCGTCACAGCTCTTTCCCATACATTATTGATGTTCGACGCTTTCTATGACGTTGAAG
AAAAAGCGAAAGCGGGTAACGTTTTTGCACAGGAAATAATACAGGCATGGGCTGATGCAAAATGGTTTTTAAAGAAACCT
GCTGTTGCAGAAAAAATGACCATGAGTGTATTTAAAGTGTCTGGGGAGACAAATACTGATGATTTGTCTCCAGCCCCCGA
TGCCTGGTCTCGTCCCGATATTCCATTACACGCTTTAGCGATGTTAAAACTTTCACGAGAAGGCATTGTTGCTGACGAGG
AAGGTGTCGTTGGACCGGTTAAACAAATTAATGCACTGAAAGATAAAGGTCACCCGTTAGTTTATGTTGGTGACGTTGTC
GGTACCGGCTCTTCGCGTAAATCTGCAACTAATTCAGTTTTATGGTTTATGGGTAACGATGTTCCATTTGTACCTAATAA
ACGTGCTGGTGGTGTGTGTATTGGTAATAAAATAGCCCCTATTTTCTTTAATACGATGGAAGATTCAGGCGCATTACCTA
TTGAGATGGATGTCAGTAAATTAAATACCGGTGATGTGATTGATATATATCCTCATCAAGGAGTTGTTAAAAACAACGCT
ACCGGTGAAGTTATTACTGAATTTCAATTAAAAACAAATATTATTCTTGATGAAGTACGTGCCGGTGGACGAATTCCACT
TATTATCGGTCGTGGTTTAACAGATCGTGCGCGTAAATCTTTAGGGCTTGAAACATCGACTGTCTTTCAATTGCCAGTTG
AAGTTAAACCTTCAACAAAAGGTTATACGCTGGCACAAAAAATGGTCGGTAAAGCTTGTGGTACTGAAGGCGTACGAGCA
GGTCAATATTGTGAGCCTAAAATGACCAGTGTTGGATCGCAAGATACAACAGGCCCAATGACACGTGATGAGCTCAAAGA
TCTTGCCTGCTTAGGTTTCTCCGCTGATTTGGTTATGCAATCATTCTGCCATACGGGTGCATACCCTAAACCCATTGATG
TTATTACGCATCACACTCTGCCTGATTTCATCCGAAATCGTGGTGGTGTTTCTCTGCGCCCTGGTGACGGTATTATCCAC
TCGTGGTTAAATCGTATGTTATTACCGGATACGGTAGGAACAGGTGGTGATTCTCATACACGTTTCCCGATCGGCATCTC
TTTCCCAGCCGGTTCAGGCTTAGTGGCATTTGCTGCAGCGACAGGTGTTATGCCGTTGGATATGCCAGAATCTGTGTTGG
TGCGTTTTAAAGGCGAAATGCAGCCAGGCATTACATTACGTGATTTAGTGAATGCAATTCCCCACCAGGCGATAAAAGAG
GGCCACTTAACGGTTGAAAAAGCGGGCAAAGTAAATGTGTTCTCTGGCCGTACTTTAGAGATTGAAGGGTTACCAAAACT
TAAAGTTGAGCAAGCATTTGAATTATCAGATGCCTCAGCAGAGCGTAGTGCTGCGGGTTGTTCGATCAAGCTTGATAAAG
AACCTATTATTGAATACTTAAACTCTAACATTGTGATGCTTAAGTGGATGATAGCCGAAGGTTATGGTGATGTTCGTACT
ATTTCTCGTCGTATTGAAGAGATGGAAGCCTGGATTGCAAAGCCTGAGTTAATGGAAGCGGATGAAGATGCAGAATATGC
CGCTATTATTGAGATTAATCTGAACGATGTTAAAGAGCCTCTTTTAGCTTGTCCAAATGATCCCGATGATGTGAAATTAC
TGTCAGAGGTTGCTGGCCAATCAATTGATGAAGTCTTCATTGGTTCTTGCATGACCAATATAGGTCATTTCCGTGCAGCA
GGTAATTTGCTCCAACAATTCCCTGGTCAATTACCGACCAAGTTATGGATTGCCCCACCAACAAAAATGGATGCTGCGCA
ACTTTCTGATGAAGGTTATTATGGCATCTTTGGTAAAGTGGGTGCACGAACTGAAATGCCGGGTTGCTCTCTTTGTATGG
GTAATCAAGCGCGAGTGGGTGATAATACCACTGTGGTTTCAACTTCAACACGTAACTTCCCTAACCGTTTAGGTAATAAT
GCTAATGTGTACCTGAGTTCAGCTGAACTCGCTGCAGTTGCCGCTATCGAAGGGAAACTGCCAACGGTTGATCAATATAT
GAAGTATGCTCAATCCTTGAATGCGACTGCCGCAGATACCTACCGTTACCTGAACTTTGATCAAATAAAAAGTTATATGG
ATAAAGCAAATAGTATTATTCCGGTGAAAGCTGCCAGCTAA

Upstream 100 bases:

>100_bases
CCTGCTGATGCGGTATAATTTTTGGGAAATTTTTATATCAAACTTATATCAAACGATTCCAATAACGCGAATTATTTCGC
TGCAACAAGAGGTTACTATT

Downstream 100 bases:

>100_bases
CAAGTATTATTTTTTTATAACTTAAAAAATTAGGCCTCTCTTCTGGTTATTCGTTGAGGGGCTTTTTTTATGATGGTAAA
TATATTATTCATTTATAATA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 866; Mature: 866

Protein sequence:

>866_residues
MLEAYRKHIAERAEQGIVPQPLNAEQTAALVELLKTPPQGEEAFLVDLLENRIPPGVDEAAYVKAGFLSAIASAETISPI
ISREHAVKLLGTMQGGYNIQPLISQLDNEKLAPLAVTALSHTLLMFDAFYDVEEKAKAGNVFAQEIIQAWADAKWFLKKP
AVAEKMTMSVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKLSREGIVADEEGVVGPVKQINALKDKGHPLVYVGDVV
GTGSSRKSATNSVLWFMGNDVPFVPNKRAGGVCIGNKIAPIFFNTMEDSGALPIEMDVSKLNTGDVIDIYPHQGVVKNNA
TGEVITEFQLKTNIILDEVRAGGRIPLIIGRGLTDRARKSLGLETSTVFQLPVEVKPSTKGYTLAQKMVGKACGTEGVRA
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTGAYPKPIDVITHHTLPDFIRNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVNAIPHQAIKE
GHLTVEKAGKVNVFSGRTLEIEGLPKLKVEQAFELSDASAERSAAGCSIKLDKEPIIEYLNSNIVMLKWMIAEGYGDVRT
ISRRIEEMEAWIAKPELMEADEDAEYAAIIEINLNDVKEPLLACPNDPDDVKLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GNLLQQFPGQLPTKLWIAPPTKMDAAQLSDEGYYGIFGKVGARTEMPGCSLCMGNQARVGDNTTVVSTSTRNFPNRLGNN
ANVYLSSAELAAVAAIEGKLPTVDQYMKYAQSLNATAADTYRYLNFDQIKSYMDKANSIIPVKAAS

Sequences:

>Translated_866_residues
MLEAYRKHIAERAEQGIVPQPLNAEQTAALVELLKTPPQGEEAFLVDLLENRIPPGVDEAAYVKAGFLSAIASAETISPI
ISREHAVKLLGTMQGGYNIQPLISQLDNEKLAPLAVTALSHTLLMFDAFYDVEEKAKAGNVFAQEIIQAWADAKWFLKKP
AVAEKMTMSVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKLSREGIVADEEGVVGPVKQINALKDKGHPLVYVGDVV
GTGSSRKSATNSVLWFMGNDVPFVPNKRAGGVCIGNKIAPIFFNTMEDSGALPIEMDVSKLNTGDVIDIYPHQGVVKNNA
TGEVITEFQLKTNIILDEVRAGGRIPLIIGRGLTDRARKSLGLETSTVFQLPVEVKPSTKGYTLAQKMVGKACGTEGVRA
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTGAYPKPIDVITHHTLPDFIRNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVNAIPHQAIKE
GHLTVEKAGKVNVFSGRTLEIEGLPKLKVEQAFELSDASAERSAAGCSIKLDKEPIIEYLNSNIVMLKWMIAEGYGDVRT
ISRRIEEMEAWIAKPELMEADEDAEYAAIIEINLNDVKEPLLACPNDPDDVKLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GNLLQQFPGQLPTKLWIAPPTKMDAAQLSDEGYYGIFGKVGARTEMPGCSLCMGNQARVGDNTTVVSTSTRNFPNRLGNN
ANVYLSSAELAAVAAIEGKLPTVDQYMKYAQSLNATAADTYRYLNFDQIKSYMDKANSIIPVKAAS
>Mature_866_residues
MLEAYRKHIAERAEQGIVPQPLNAEQTAALVELLKTPPQGEEAFLVDLLENRIPPGVDEAAYVKAGFLSAIASAETISPI
ISREHAVKLLGTMQGGYNIQPLISQLDNEKLAPLAVTALSHTLLMFDAFYDVEEKAKAGNVFAQEIIQAWADAKWFLKKP
AVAEKMTMSVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKLSREGIVADEEGVVGPVKQINALKDKGHPLVYVGDVV
GTGSSRKSATNSVLWFMGNDVPFVPNKRAGGVCIGNKIAPIFFNTMEDSGALPIEMDVSKLNTGDVIDIYPHQGVVKNNA
TGEVITEFQLKTNIILDEVRAGGRIPLIIGRGLTDRARKSLGLETSTVFQLPVEVKPSTKGYTLAQKMVGKACGTEGVRA
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTGAYPKPIDVITHHTLPDFIRNRGGVSLRPGDGIIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEMQPGITLRDLVNAIPHQAIKE
GHLTVEKAGKVNVFSGRTLEIEGLPKLKVEQAFELSDASAERSAAGCSIKLDKEPIIEYLNSNIVMLKWMIAEGYGDVRT
ISRRIEEMEAWIAKPELMEADEDAEYAAIIEINLNDVKEPLLACPNDPDDVKLLSEVAGQSIDEVFIGSCMTNIGHFRAA
GNLLQQFPGQLPTKLWIAPPTKMDAAQLSDEGYYGIFGKVGARTEMPGCSLCMGNQARVGDNTTVVSTSTRNFPNRLGNN
ANVYLSSAELAAVAAIEGKLPTVDQYMKYAQSLNATAADTYRYLNFDQIKSYMDKANSIIPVKAAS

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=860, Percent_Identity=76.2790697674419, Blast_Score=1360, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=391, Percent_Identity=24.0409207161125, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=26.509186351706, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93631; Mature: 93631

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHIAERAEQGIVPQPLNAEQTAALVELLKTPPQGEEAFLVDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLSAIASAETISPIISREHAVKLLGTMQGGYNIQPLISQLDNEKLAPLAVTALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHH
HTLLMFDAFYDVEEKAKAGNVFAQEIIQAWADAKWFLKKPAVAEKMTMSVFKVSGETNTD
HHHHHHHHHHCHHHHHHHCHHHHHHHHHHHCCCHHHHCCCCHHHHHEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKLSREGIVADEEGVVGPVKQINALKDKGHPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCEE
GTGSSRKSATNSVLWFMGNDVPFVPNKRAGGVCIGNKIAPIFFNTMEDSGALPIEMDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEECEEEEEECCCCCCCEEEEEHHH
LNTGDVIDIYPHQGVVKNNATGEVITEFQLKTNIILDEVRAGGRIPLIIGRGLTDRARKS
CCCCCEEEECCCCCEECCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LGLETSTVFQLPVEVKPSTKGYTLAQKMVGKACGTEGVRAGQYCEPKMTSVGSQDTTGPM
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLVMQSFCHTGAYPKPIDVITHHTLPDFIRNRGGVSLRPGDGIIH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHCCCCEEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVNAIPHQAIKEGHLTVEKAGKVNVFSGRTLEIEGLPKLKVEQAFELSDASA
CCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCEEEECCCCCCCHHHHHCCCCCCC
ERSAAGCSIKLDKEPIIEYLNSNIVMLKWMIAEGYGDVRTISRRIEEMEAWIAKPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCC
DEDAEYAAIIEINLNDVKEPLLACPNDPDDVKLLSEVAGQSIDEVFIGSCMTNIGHFRAA
CCCCCEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GNLLQQFPGQLPTKLWIAPPTKMDAAQLSDEGYYGIFGKVGARTEMPGCSLCMGNQARVG
HHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCEECCCCCCCC
DNTTVVSTSTRNFPNRLGNNANVYLSSAELAAVAAIEGKLPTVDQYMKYAQSLNATAADT
CCCEEEECCCCCCHHHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
YRYLNFDQIKSYMDKANSIIPVKAAS
HHHCCHHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure
MLEAYRKHIAERAEQGIVPQPLNAEQTAALVELLKTPPQGEEAFLVDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLSAIASAETISPIISREHAVKLLGTMQGGYNIQPLISQLDNEKLAPLAVTALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHH
HTLLMFDAFYDVEEKAKAGNVFAQEIIQAWADAKWFLKKPAVAEKMTMSVFKVSGETNTD
HHHHHHHHHHCHHHHHHHCHHHHHHHHHHHCCCHHHHCCCCHHHHHEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKLSREGIVADEEGVVGPVKQINALKDKGHPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCEE
GTGSSRKSATNSVLWFMGNDVPFVPNKRAGGVCIGNKIAPIFFNTMEDSGALPIEMDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEECEEEEEECCCCCCCEEEEEHHH
LNTGDVIDIYPHQGVVKNNATGEVITEFQLKTNIILDEVRAGGRIPLIIGRGLTDRARKS
CCCCCEEEECCCCCEECCCCCCCEEEEEEHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
LGLETSTVFQLPVEVKPSTKGYTLAQKMVGKACGTEGVRAGQYCEPKMTSVGSQDTTGPM
CCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLVMQSFCHTGAYPKPIDVITHHTLPDFIRNRGGVSLRPGDGIIH
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHHCCCCEEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVNAIPHQAIKEGHLTVEKAGKVNVFSGRTLEIEGLPKLKVEQAFELSDASA
CCCCCHHHHHHHHHHHHHHCCCEEEECCCEEEEECCCEEEECCCCCCCHHHHHCCCCCCC
ERSAAGCSIKLDKEPIIEYLNSNIVMLKWMIAEGYGDVRTISRRIEEMEAWIAKPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCHHHCC
DEDAEYAAIIEINLNDVKEPLLACPNDPDDVKLLSEVAGQSIDEVFIGSCMTNIGHFRAA
CCCCCEEEEEEEEHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GNLLQQFPGQLPTKLWIAPPTKMDAAQLSDEGYYGIFGKVGARTEMPGCSLCMGNQARVG
HHHHHHCCCCCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCEECCCCCCCC
DNTTVVSTSTRNFPNRLGNNANVYLSSAELAAVAAIEGKLPTVDQYMKYAQSLNATAADT
CCCEEEECCCCCCHHHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCHHHH
YRYLNFDQIKSYMDKANSIIPVKAAS
HHHCCHHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]